Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F55 I0 R1	519	344.2	11360120	95.0%	10792114	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	766,563	G→A	11.1%	intergenic (+496/‑351)	mngB → / → cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	766,563	0	G	A	11.1%	980.3 / 85.8	317	intergenic (+496/‑351)	mngB/cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand): ref base G (167/113); new base A (12/23); total (179/138)
Fisher's exact test for biased strand distribution p-value = 6.08e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 321 total aligned reads displayed.

AAGAATTAAGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATT  >  CP009273/766414‑766712

aaGAATTAAGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAgg                                                                                                                                                       >  1:4832375/1‑150 (MQ=255)

  gAATTAAGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGaa                                                                                                                                                     <  1:5607675/150‑1 (MQ=255)

   aaTTAAGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAAt                                                                                                                                                    >  2:4904527/1‑150 (MQ=255)

         ggTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGatat                                                                                                                                              <  1:5433496/150‑1 (MQ=255)

          gTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATAtt                                                                                                                                             >  1:582630/1‑150 (MQ=255)

          gTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATAtt                                                                                                                                             >  2:352651/1‑150 (MQ=255)

             aaCCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATAAACATAATTTGTAGAAATTGATATTTAt                                                                                                                                          >  2:60325/1‑150 (MQ=255)

              aCCGTTCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATc                                                                                                                                         <  2:5164247/150‑1 (MQ=255)

               ccGTGCTGATTTTGCTTCGTCTCTTTTTATCTATAATTGCCAACCGACACCAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAACCTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCa                                                                                                                                        <  2:3057252/150‑1 (MQ=255)

                 gTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAAt                                                                                                                                      >  2:3239050/1‑150 (MQ=255)

                 gTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAAAATAATTTGTAGGAATTGATATTTATCAAt                                                                                                                                      >  2:2134863/1‑150 (MQ=255)

                  tGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTACCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATg                                                                                                                                     >  1:3418064/1‑150 (MQ=255)

                    cTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGta                                                                                                                                   >  2:3982728/1‑150 (MQ=255)

                        ttttGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAg                                                                                                                               >  2:3701750/1‑150 (MQ=255)

                        ttttGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGatat                                                                                                                                              <  2:3349708/135‑1 (MQ=255)

                        ttttGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGatat                                                                                                                                              >  1:3349708/1‑135 (MQ=255)

                         tttGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGt                                                                                                                              >  2:223952/1‑150 (MQ=255)

                          ttGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTc                                                                                                                             <  2:1851301/150‑1 (MQ=255)

                          ttGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTc                                                                                                                             <  2:2446089/150‑1 (MQ=255)

                          ttGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTc                                                                                                                             <  1:199383/150‑1 (MQ=255)

                            gCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCtt                                                                                                                           >  1:2073720/1‑150 (MQ=255)

                              ttCGTCTCTTTTTATCTTTAAGTGCCAACCGAAAATAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTgg                                                                                                                         <  1:3414820/150‑1 (MQ=255)

                               tCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAATTCTTGGa                                                                                                                        >  2:1081925/1‑150 (MQ=255)

                                 gTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACATTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGaaa                                                                                                                      >  1:3661425/1‑150 (MQ=255)

                                   ctctTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATg                                                                                                                    >  1:4277246/1‑150 (MQ=255)

                                    tctTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATgg                                                                                                                   <  2:3457607/150‑1 (MQ=255)

                                    tctTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATgg                                                                                                                   >  1:2159675/1‑150 (MQ=255)

                                     ctTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACTTAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATggg                                                                                                                  >  2:4629337/1‑150 (MQ=255)

                                     ctTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATggg                                                                                                                  <  1:3397091/150‑1 (MQ=255)

                                       ttttATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCa                                                                                                                >  2:2649395/1‑150 (MQ=255)

                                       ttttATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCa                                                                                                                >  2:5039630/1‑150 (MQ=255)

                                          tATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCa                                                                                                             >  2:316828/1‑150 (MQ=255)

                                           aTCATTTATGGCCACCCGAAACTAATTTCAGCCTTATAACTCACCCATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCac                                                                                                            <  1:2931186/150‑2 (MQ=255)

                                              tttAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCaaaaa                                                                                                         <  2:245891/150‑1 (MQ=255)

                                                 aaTTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAagag                                                                                                      >  1:1805644/1‑150 (MQ=255)

                                                   ttGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGAt                                                                                                    <  1:5345433/150‑1 (MQ=255)

                                                     gCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATCAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATaa                                                                                                  <  1:3051800/150‑1 (MQ=255)

                                                     gCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATaa                                                                                                  >  2:1261349/1‑150 (MQ=255)

                                                        aaCCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAAtt                                                                                               <  1:4096226/150‑1 (MQ=255)

                                                        aaCCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAAtt                                                                                               >  1:3134079/1‑150 (MQ=255)

                                                          ccGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGt                                                                                             >  2:160147/1‑150 (MQ=255)

                                                            gAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTtc                                                                                           <  2:4593970/150‑1 (MQ=255)

                                                             aaaCTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTtct                                                                                          >  1:742967/1‑150 (MQ=255)

                                                              aaCTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTtctc                                                                                         <  1:4019275/150‑1 (MQ=255)

                                                               aatAATTTAAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTTACATAATTTGTATGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCg                                                                                        <  1:3771550/148‑1 (MQ=255)

                                                               aCTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCg                                                                                        >  1:4891974/1‑150 (MQ=255)

                                                                  aaTTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATc                                                                                     >  2:4411907/1‑150 (MQ=255)

                                                                  aaTTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATc                                                                                     <  1:1557258/150‑1 (MQ=255)

                                                                       cAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAAtt                                                                                <  2:4678576/150‑1 (MQ=255)

                                                                        aGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTg                                                                               >  2:2001637/1‑150 (MQ=255)

                                                                        aGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTg                                                                               <  1:3097565/150‑1 (MQ=255)

                                                                          ccTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGc                                                                             <  1:5040831/150‑1 (MQ=255)

                                                                           cTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCt                                                                            >  1:4638804/1‑150 (MQ=255)

                                                                           cTTATAACTCACACAATTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCt                                                                            <  2:2744226/150‑1 (MQ=255)

                                                                            ttATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTg                                                                           >  1:116222/1‑150 (MQ=255)

                                                                             tataACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGa                                                                          >  1:4998074/1‑150 (MQ=255)

                                                                              ataACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGaa                                                                         >  1:2501682/1‑150 (MQ=255)

                                                                                aaCTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAg                                                                       >  1:1703658/1‑150 (MQ=255)

                                                                                 aCTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAgg                                                                      <  1:352651/150‑1 (MQ=255)

                                                                                  cTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTATCGATCAAATTGGCTGaaatga                                                                     >  2:333475/1‑147 (MQ=255)

                                                                                    cacacaTTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGATATAAATGGTTCTCGATCAAATTGGCTGAAAGGCgg                                                                   >  2:5226462/1‑150 (MQ=255)

                                                                                      cacaTTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTa                                                                 <  1:1480834/150‑1 (MQ=255)

                                                                                       acaTTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTaa                                                                >  1:766485/1‑150 (MQ=255)

                                                                                       acaTTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTaa                                                                <  1:2880577/150‑1 (MQ=255)

                                                                                          ttttAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAAttt                                                             <  1:4943665/150‑1 (MQ=255)

                                                                                            ttAAACATAAATGTCACTAAATTTACCTTATTGAAACATGATTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAg                                                           <  2:2826654/150‑1 (MQ=255)

                                                                                            ttAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAg                                                           >  2:5019054/1‑150 (MQ=255)

                                                                                            ttAAACATAAATGTAACTAAAGTTACATTATTTAAACATGATTAACATAATTTGTAGGAATTTATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGTCGGTAATTTAg                                                           <  2:4033653/150‑1 (MQ=255)

                                                                                              aaaCATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCt                                                         <  2:5427367/150‑1 (MQ=255)

                                                                                              aaaCATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCt                                                         >  2:645791/1‑150 (MQ=255)

                                                                                              aaaCATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCt                                                         <  2:4237631/150‑1 (MQ=255)

                                                                                               aaCATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCta                                                        <  2:2008759/150‑1 (MQ=255)

                                                                                               aaCATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCta                                                        >  1:1938565/1‑150 (MQ=255)

                                                                                                   tAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATaaa                                                    >  1:3966388/1‑150 (MQ=255)

                                                                                                   tAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATaaa                                                    >  1:4133755/1‑150 (MQ=255)

                                                                                                       tGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGa                                                <  1:1369344/150‑1 (MQ=255)

                                                                                                            cTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCAcc                                           <  2:4084348/150‑1 (MQ=255)

                                                                                                             tAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCg                                          >  2:1417802/1‑150 (MQ=255)

                                                                                                             tAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCg                                          >  2:4093081/1‑150 (MQ=255)

                                                                                                              aaaGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGt                                         <  2:3900972/150‑1 (MQ=255)

                                                                                                               aaGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTc                                        >  2:1194929/1‑150 (MQ=255)

                                                                                                                 gTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGa                                      >  1:4194965/1‑150 (MQ=255)

                                                                                                                     ccTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGaaaaa                                  >  1:3016856/1‑150 (MQ=255)

                                                                                                                     ccTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGaaaaa                                  >  1:3841846/1‑150 (MQ=255)

                                                                                                                             aaaCATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAAtt                          >  1:4495376/1‑150 (MQ=255)

                                                                                                                             aaaCATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAAtt                          >  2:2206021/1‑150 (MQ=255)

                                                                                                                               aCATTATTAACATAATTTGTAGGAATTGATATTTATCACTGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTg                        <  2:4770764/150‑1 (MQ=255)

                                                                                                                               aCATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTg                        <  2:1938889/150‑1 (MQ=255)

                                                                                                                               aCATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGAAAATTTg                        >  2:1800593/1‑150 (MQ=255)

                                                                                                                                 aTGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCt                      <  1:5186956/150‑1 (MQ=255)

                                                                                                                                   gATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTc                    >  2:173404/1‑150 (MQ=255)

                                                                                                                                   gATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTc                    >  1:2736888/1‑150 (MQ=255)

                                                                                                                                    aTTAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTca                   <  1:2293575/150‑1 (MQ=255)

                                                                                                                                     ttAACATAATTTGTAGAAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTcaa                  <  2:1048946/150‑1 (MQ=255)

                                                                                                                                       aaCATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAATGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAATATGCAAATTTGCTTcaaca                >  2:2814865/1‑150 (MQ=255)

                                                                                                                                        aCATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTcaacaa               <  2:575031/150‑1 (MQ=255)

                                                                                                                                         cATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACaaa              <  2:1867171/150‑1 (MQ=255)

                                                                                                                                                tGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGATATAAATTGTTCTCGATCAAATTGGCTGTAAGGAGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACATGt       >  2:2344769/1‑150 (MQ=255)

                                                                                                                                                 gTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGtt      <  1:2805465/150‑1 (MQ=255)

                                                                                                                                                  tAGTAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGttt     <  2:3632064/150‑1 (MQ=255)

AAGAATTAAGGTCAACCGTGCTGTTTTTGCTTCGTCTCTTTTTATCTTTAATTGCCAACCGAAACTAATTTCAGCCTTATAACTCACACATTTTAAACATAAATGTCACTAAAGTTACCTTATTGAAACATGATTAACATAATTTGTAGGAATTGATATTTATCAATGTATAAGTCTTGGAAATGGGCATCAAAAAGAGATAAATTGTTCTCGATCAAATTGGCTGAAAGGCGGTAATTTAGCTATAAATTGATCACCGTCGAAAAATGCAAATTTGCTTCAACAAAAACCTGTTTATT  >  CP009273/766414‑766712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A