Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F83 I1 R1 393 530.9 9427126 91.1% 8588111 148.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,885,813 2 bp→CC coding (39‑40/351 nt) FTN78_chr1931 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,885,8130TC99.6% 499.9 / NA 266I13I (ATT→ATC) FTN78_chr1931hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (133/132);  minor base A (0/1);  total (133/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0435231,885,8140AC99.2% 503.6 / NA 266R14R (AGG→CGG) FTN78_chr1931hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base C (133/131);  total (133/133)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

AAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTAT‑TAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTAC  >  CP043523/1885689‑1885931
                                                                                                                             ||                                                                                                                      
  gtagtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGatga                                                                                               >  2:4354096/1‑148 (MQ=14)
     gtTTATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGAtgatga                                                                                            >  1:4094771/1‑148 (MQ=9)
      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                           <  1:2160498/148‑1 (MQ=9)
      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGaa                                                                                           <  1:3016658/148‑1 (MQ=9)
      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGaa                                                                                           <  2:2239899/148‑1 (MQ=14)
      tttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGaa                                                                                           >  1:530145/1‑148 (MQ=14)
       ttATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGaaa                                                                                          <  1:1696591/148‑1 (MQ=9)
        tATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAAt                                                                                         >  1:112008/1‑148 (MQ=9)
        tATCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAAt                                                                                         >  1:2548492/1‑148 (MQ=9)
         aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATg                                                                                        <  1:3465910/148‑1 (MQ=9)
         aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATg                                                                                        <  1:2633136/148‑1 (MQ=9)
         aTCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATg                                                                                        >  2:3957817/1‑148 (MQ=14)
          tCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgg                                                                                       >  2:411461/1‑148 (MQ=9)
          tCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgg                                                                                       <  1:1212382/148‑1 (MQ=9)
          tCCGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgg                                                                                       >  1:2920411/1‑148 (MQ=9)
            cgaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                     <  1:449889/141‑1 (MQ=9)
            cGAGGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATgggg                                                                                     <  1:206999/148‑1 (MQ=14)
             gaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATggggc                                                                                    >  2:1231262/7‑147 (MQ=9)
             gaggattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATggggc                                                                                    >  1:2313519/7‑147 (MQ=14)
                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 <  1:1062907/145‑1 (MQ=9)
                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 <  1:4459021/145‑1 (MQ=9)
                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  2:243568/4‑148 (MQ=14)
                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 <  1:1576746/145‑1 (MQ=9)
                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  1:1504845/4‑148 (MQ=14)
                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  2:2743521/4‑148 (MQ=14)
                gattCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCa                                                                                 >  1:1035787/4‑148 (MQ=14)
                 attCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAt                                                                                >  2:1154676/3‑148 (MQ=14)
                  ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                               <  1:4353908/147‑1 (MQ=14)
                  ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                               <  1:1761469/147‑1 (MQ=14)
                  ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                               >  1:3158717/2‑148 (MQ=14)
                  ttCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCAtt                                                                               >  1:4345688/2‑148 (MQ=14)
                    cACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTg                                                                             <  1:752226/148‑1 (MQ=14)
                     aCGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGc                                                                            >  2:3479348/1‑148 (MQ=14)
                      cGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCg                                                                           >  2:4221398/1‑148 (MQ=14)
                       gtgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGa                                                                          >  2:1415097/1‑148 (MQ=14)
                       gtgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGa                                                                          <  2:4062288/148‑1 (MQ=14)
                        tgtCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGAc                                                                         >  2:2986703/1‑148 (MQ=14)
                           cATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgg                                                                      >  1:964130/1‑148 (MQ=14)
                            aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgga                                                                     <  2:1714070/148‑2 (MQ=14)
                            aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTgga                                                                     <  1:4044225/148‑2 (MQ=14)
                            aTTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAATCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTTCGACTgga                                                                     >  2:808044/1‑147 (MQ=14)
                             tttATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                    <  1:2322851/148‑3 (MQ=9)
                             tttATCGGAACAAACTCATTGGAGGACATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTggag                                                                    >  2:3702487/1‑146 (MQ=255)
                                 tCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaact                                                                >  1:495335/1‑146 (MQ=14)
                                  cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                               <  2:4275548/148‑4 (MQ=9)
                                  cGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactc                                                               >  2:3189738/1‑145 (MQ=14)
                                   ggAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactca                                                              >  2:2870570/1‑144 (MQ=14)
                                     aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            <  1:35277/148‑7 (MQ=9)
                                     aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            <  2:3553693/148‑7 (MQ=9)
                                     aaCAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGaactcacg                                                            >  2:3764657/1‑142 (MQ=9)
                                                    ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             >  1:1363456/1‑148 (MQ=2)
                                                    ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             <  2:1993585/148‑1 (MQ=255)
                                                    ggTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACt                                             <  1:4088061/148‑1 (MQ=255)
                                                      tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           <  1:4681264/148‑1 (MQ=2)
                                                      tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           >  1:243556/1‑148 (MQ=2)
                                                      tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           <  1:3719186/148‑1 (MQ=2)
                                                      tCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACttt                                           >  2:2107114/1‑148 (MQ=2)
                                                       cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                          >  1:1121449/1‑148 (MQ=2)
                                                       cATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACtttt                                          >  1:3131464/1‑148 (MQ=9)
                                                        aTGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGGCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTg                                         <  1:3350338/148‑1 (MQ=255)
                                                          gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       <  2:961564/148‑1 (MQ=9)
                                                          gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       >  2:4071376/1‑148 (MQ=9)
                                                          gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       >  1:3268670/1‑148 (MQ=9)
                                                          gcagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaa                                       <  2:3030589/148‑1 (MQ=9)
                                                           cagcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaa                                      <  1:3052059/148‑1 (MQ=9)
                                                            agcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaa                                     <  1:1631205/148‑1 (MQ=255)
                                                            agcaAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaa                                     >  1:4285257/1‑148 (MQ=9)
                                                              caAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaag                                   >  1:707391/1‑148 (MQ=9)
                                                               aaGTCAAGACAAGGAGTTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaga                                  <  2:1526055/148‑1 (MQ=9)
                                                                aGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaa                                 >  2:857055/1‑148 (MQ=9)
                                                                 gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                <  1:1417187/148‑1 (MQ=9)
                                                                 gTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaa                                <  1:1314753/148‑1 (MQ=9)
                                                                  tCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTTCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaa                               >  1:700408/1‑148 (MQ=9)
                                                                  tCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaa                               <  1:387898/148‑1 (MQ=9)
                                                                   cAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCAT‑GCT‑TATAACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaaaaaaaa                              <  2:2577637/148‑1 (MQ=9)
                                                                   cAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaaa                              <  2:2049074/148‑1 (MQ=9)
                                                                   cAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTgaaaaagaaaaa                              >  1:3617556/1‑148 (MQ=9)
                                                                      gACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGaaaaaaaa                           >  2:4666855/1‑148 (MQ=9)
                                                                       aCAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaag                          >  2:1881852/1‑148 (MQ=9)
                                                                        cAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGGCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGTATGTTGAAATGGGGCGCAGTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAaaga                         <  1:4157984/148‑1 (MQ=255)
                                                                           ggaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAagatga                      <  1:1884460/148‑1 (MQ=12)
                                                                            gaggtAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAgatgat                     <  1:3827059/148‑1 (MQ=255)
                                                                              ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                   <  1:2410604/145‑1 (MQ=9)
                                                                              ggtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataa                   >  2:2323879/4‑148 (MQ=9)
                                                                               gtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                  >  1:2293771/3‑148 (MQ=9)
                                                                               gtaaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataag                  >  1:839984/3‑148 (MQ=9)
                                                                                taaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga                 >  2:1468276/2‑148 (MQ=9)
                                                                                taaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAAAGatgataaga                 <  2:1372082/147‑1 (MQ=255)
                                                                                taaaaGATGTCAGTTGACCGTTAATACAGCCAATATGAAGTAAT‑ACG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAAAGatgataaga                 <  2:618916/147‑1 (MQ=255)
                                                                                taaaaGATGGCAGTTGACCCTTCATACAGCCCCTATGAAGACAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTTGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataaga                 <  2:2794461/147‑1 (MQ=255)
                                                                                 aaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaa                <  1:1981747/148‑2 (MQ=9)
                                                                                 aaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaa                <  1:2648924/148‑2 (MQ=9)
                                                                                 aaaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGACAT‑CCG‑TGCTAATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAAAAAAAAAAGatgataagaa                <  2:4549686/148‑2 (MQ=9)
                                                                                  aaaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGatgataagaaa               <  2:1031804/148‑3 (MQ=9)
                                                                                   aaGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATgataagaaaa              <  1:2859944/148‑1 (MQ=9)
                                                                                    aGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAt             >  1:2720011/1‑148 (MQ=9)
                                                                                     gATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCGTGCT‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAtt            >  1:959565/1‑148 (MQ=255)
                                                                                      aTGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTc           >  2:461221/1‑148 (MQ=255)
                                                                                         tCAGTTGACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAg        <  2:1772092/148‑1 (MQ=255)
                                                                                               gACCGTTCATACAGCCCCTATGAAGTCAT‑CCG‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTAc  <  2:2389290/148‑1 (MQ=255)
                                                                                                                             ||                                                                                                                      
AAGTAGTTTATCCGAGGGATCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTAT‑TAG‑GATGTATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTAC  >  CP043523/1885689‑1885931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGAGGGA‑TCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTATTAGGATG‑‑TATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAAT  >  CP043523/1885699‑1885920
                                                                                                                   |                                                                                                             
TCCGAGGAT‑TCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGG                                                                          >  SRR13214404.2352385/1‑150 (MQ=37)
TCCGAGGAT‑TCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGG                                                                          >  SRR13214404.3028176/1‑150 (MQ=37)
TCCGAGGAT‑TCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGG                                                                          <  SRR13214404.1220950/150‑1 (MQ=37)
TCCGAGGAT‑TCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGG                                                                          >  SRR13214404.1609615/1‑150 (MQ=37)
      GGATTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCAT                                                                     <  SRR13214404.2281034/150‑1 (MQ=37)
       gatTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATT                                                                    <  SRR13214404.1071003/147‑1 (MQ=38)
       gatTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATT                                                                    <  SRR13214404.1070465/147‑1 (MQ=38)
       gatTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATT                                                                    >  SRR13214404.1913576/4‑150 (MQ=38)
       gatTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATT                                                                    >  SRR13214404.3178839/4‑150 (MQ=38)
       gatTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATT                                                                    <  SRR13214404.4486896/147‑1 (MQ=38)
       gatTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATT                                                                    <  SRR13214404.1587693/147‑1 (MQ=38)
       gatTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATT                                                                    >  SRR13214404.4373341/4‑150 (MQ=38)
       gatTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATT                                                                    >  SRR13214404.4372958/4‑150 (MQ=38)
        atTCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTT                                                                   <  SRR13214404.4230771/148‑1 (MQ=37)
        T‑TCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTG                                                                  <  SRR13214404.4139726/150‑1 (MQ=37)
        T‑TCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTG                                                                  <  SRR13214404.1773589/150‑1 (MQ=37)
        T‑TCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTG                                                                  <  SRR13214404.4381222/150‑1 (MQ=37)
           CACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCG                                                                <  SRR13214404.757550/150‑1 (MQ=40)
           CACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCG                                                                >  SRR13214404.2448391/1‑150 (MQ=40)
           CACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCG                                                                <  SRR13214404.27891/150‑1 (MQ=40)
           CACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCG                                                                <  SRR13214404.4281443/150‑1 (MQ=40)
              GTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACT                                                             >  SRR13214404.2328752/1‑150 (MQ=41)
                GTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGG                                                           >  SRR13214404.970777/1‑150 (MQ=41)
                  CATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAG                                                         >  SRR13214404.3331696/1‑150 (MQ=38)
                   ATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGA                                                        <  SRR13214404.4069739/150‑1 (MQ=35)
                   ATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGA                                                        >  SRR13214404.3063254/1‑150 (MQ=35)
                   ATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGA                                                        <  SRR13214404.2956205/150‑1 (MQ=35)
                    TTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAA                                                       <  SRR13214404.2338204/150‑1 (MQ=35)
                     TTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaac                                                      <  SRR13214404.1941374/150‑6 (MQ=35)
                      TATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACT                                                     >  SRR13214404.498715/1‑150 (MQ=33)
                      TATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACT                                                     >  SRR13214404.498826/1‑150 (MQ=33)
                       ATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactc                                                    >  SRR13214404.4570288/1‑143 (MQ=32)
                       ATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactc                                                    <  SRR13214404.967780/150‑8 (MQ=32)
                        TCGGAACAACCTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactca                                                   <  SRR13214404.3152961/150‑9 (MQ=24)
                        TCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactca                                                   <  SRR13214404.1619484/150‑9 (MQ=31)
                           GAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacgg                                                >  SRR13214404.968188/1‑139 (MQ=27)
                            AACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacgga                                               >  SRR13214404.1800400/1‑138 (MQ=25)
                            AACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacgga                                               <  SRR13214404.35541/150‑13 (MQ=25)
                              CAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggagg                                             >  SRR13214404.987434/1‑136 (MQ=22)
                              CAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggagg                                             <  SRR13214404.3169037/150‑15 (MQ=22)
                               AAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggc                                            <  SRR13214404.4629945/150‑16 (MQ=21)
                               AAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggc                                            >  SRR13214404.3601058/1‑135 (MQ=21)
                                AACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggct                                           <  SRR13214404.2115388/150‑17 (MQ=21)
                                 ACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctc                                          <  SRR13214404.326645/150‑18 (MQ=19)
                                 ACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctc                                          >  SRR13214404.1232644/1‑133 (MQ=19)
                                 ACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctc                                          >  SRR13214404.2883099/1‑133 (MQ=19)
                                  CTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctca                                         >  SRR13214404.231881/1‑132 (MQ=18)
                                  CTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctca                                         >  SRR13214404.71958/1‑132 (MQ=18)
                                  CTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctca                                         >  SRR13214404.3902941/1‑132 (MQ=18)
                                  CTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctca                                         <  SRR13214404.3972158/150‑19 (MQ=18)
                                   TCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaa                                        <  SRR13214404.2659960/150‑20 (MQ=16)
                                     ATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaa                                      <  SRR13214404.3578341/150‑22 (MQ=13)
                                     ATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaa                                      <  SRR13214404.3581102/150‑22 (MQ=13)
                                     ATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaa                                      >  SRR13214404.2667573/1‑129 (MQ=13)
                                      TTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaat                                     >  SRR13214404.527043/1‑128 (MQ=13)
                                      TTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaat                                     <  SRR13214404.1672447/150‑23 (MQ=13)
                                      TTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaat                                     <  SRR13214404.2130294/150‑23 (MQ=13)
                                      TTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaat                                     <  SRR13214404.4127353/150‑23 (MQ=13)
                                       TGGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaata                                    <  SRR13214404.2865856/150‑24 (MQ=13)
                                        GGAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGAGCATACAGCCCCTAAGAAGTCATCCGTACT‑‑TATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaatac                                   <  SRR13214404.1214735/150‑25 (MQ=28)
                                         GAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaatact                                  >  SRR13214404.1373051/1‑125 (MQ=13)
                                         GAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaatact                                  >  SRR13214404.1373847/1‑125 (MQ=13)
                                         GAGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTGGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGagaactcacggaggctcaaaatact                                  <  SRR13214404.2479824/150‑26 (MQ=13)
                                          AGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTT                                 >  SRR13214404.4372874/1‑150 (MQ=11)
                                          AGGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTT                                 <  SRR13214404.3494515/150‑1 (MQ=11)
                                           GGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTT                                <  SRR13214404.4113816/150‑1 (MQ=11)
                                           GGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTT                                <  SRR13214404.4442472/150‑1 (MQ=11)
                                           GGTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTT                                >  SRR13214404.245345/1‑150 (MQ=45)
                                            GTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTT                               >  SRR13214404.1129406/1‑150 (MQ=44)
                                            GTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTT                               >  SRR13214404.3561019/1‑150 (MQ=44)
                                            GTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTT                               >  SRR13214404.1493369/1‑150 (MQ=44)
                                            GTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTT                               >  SRR13214404.1129407/1‑150 (MQ=44)
                                            GTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTT                               >  SRR13214404.3151429/1‑150 (MQ=44)
                                            GTCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTT                               >  SRR13214404.3163433/1‑150 (MQ=44)
                                             TCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTG                              <  SRR13214404.1713935/150‑1 (MQ=42)
                                             TCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTG                              <  SRR13214404.4710483/150‑1 (MQ=42)
                                             TCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTG                              <  SRR13214404.3742060/150‑1 (MQ=42)
                                             TCATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTG                              <  SRR13214404.3742765/150‑1 (MQ=42)
                                               ATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAA                            >  SRR13214404.3289507/1‑150 (MQ=41)
                                               ATGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAA                            >  SRR13214404.4094862/1‑150 (MQ=41)
                                                TGCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTGCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGTCGCATTTGCGACTGAAGAACTCACTGAGGCTCAAAATACTTGTGAAA                           >  SRR13214404.1529009/1‑150 (MQ=23)
                                                 GCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAA                          >  SRR13214404.1078975/1‑150 (MQ=41)
                                                 GCAGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAA                          >  SRR13214404.4312165/1‑150 (MQ=41)
                                                   AGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAG                        <  SRR13214404.1642506/150‑1 (MQ=41)
                                                   AGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAG                        <  SRR13214404.3418326/150‑1 (MQ=41)
                                                   AGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAG                        <  SRR13214404.218216/150‑1 (MQ=41)
                                                   AGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAG                        >  SRR13214404.4072125/1‑150 (MQ=41)
                                                   AGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAG                        >  SRR13214404.712420/1‑150 (MQ=41)
                                                   AGCAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACGTTTGAAAAAG                        >  SRR13214404.711654/1‑150 (MQ=33)
                                                     CAAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAA                      >  SRR13214404.66031/1‑150 (MQ=20)
                                                      AAGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAA                     <  SRR13214404.2337010/150‑1 (MQ=41)
                                                       AGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTTCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAA                    >  SRR13214404.705390/1‑150 (MQ=33)
                                                       AGTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAA                    <  SRR13214404.2661490/150‑1 (MQ=41)
                                                        GTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAA                   >  SRR13214404.3640529/1‑150 (MQ=41)
                                                        GTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAA                   <  SRR13214404.1324032/150‑1 (MQ=41)
                                                        GTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAA                   <  SRR13214404.1427308/150‑1 (MQ=41)
                                                        GTCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAA                   <  SRR13214404.1427100/150‑1 (MQ=41)
                                                         TCAAGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAA                  <  SRR13214404.390645/150‑1 (MQ=41)
                                                            AGACAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAG               >  SRR13214404.4421726/1‑150 (MQ=41)
                                                               CAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGGCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGTATGTTGAAATGGGGCGCAGTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATG            <  SRR13214404.4184125/150‑1 (MQ=14)
                                                               CAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATG            <  SRR13214404.345607/150‑1 (MQ=17)
                                                               CAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATG            <  SRR13214404.3241421/150‑1 (MQ=17)
                                                               CAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATG            >  SRR13214404.1832329/1‑150 (MQ=38)
                                                               CAAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATG            <  SRR13214404.2411049/150‑1 (MQ=17)
                                                                AAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGA           <  SRR13214404.3942363/150‑1 (MQ=16)
                                                                AAGGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGA           <  SRR13214404.2481431/150‑1 (MQ=16)
                                                                  GGAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATA         <  SRR13214404.1897359/150‑1 (MQ=36)
                                                                   GAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAA        <  SRR13214404.1668553/150‑1 (MQ=38)
                                                                   GAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAA        <  SRR13214404.3851325/150‑1 (MQ=38)
                                                                   GAGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAA        >  SRR13214404.1164050/1‑150 (MQ=38)
                                                                    AGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAG       >  SRR13214404.1400885/1‑150 (MQ=39)
                                                                    AGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAG       >  SRR13214404.845866/1‑150 (MQ=39)
                                                                    AGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAG       <  SRR13214404.803760/150‑1 (MQ=39)
                                                                    AGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAG       <  SRR13214404.803712/150‑1 (MQ=39)
                                                                    AGGTAAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAG       >  SRR13214404.2308970/1‑150 (MQ=39)
                                                                        AAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAA   >  SRR13214404.726762/1‑150 (MQ=41)
                                                                        AAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAA   <  SRR13214404.2666078/150‑1 (MQ=41)
                                                                        AAAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAA   <  SRR13214404.1995212/150‑1 (MQ=41)
                                                                         AAAGATGTCAGTTGACCGTTCATACAGCCCCTATGAAGTCATCCG‑‑TGCTTATCACGATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAAT  >  SRR13214404.2737553/1‑150 (MQ=20)
                                                                                                                   |                                                                                                             
TCCGAGGGA‑TCACGTGTCATTTATCGGAACAAACTCATTGGAGGTCATGCAGCAAGTCAAGACAAGGAGGAAAAAGATGTCAGTTGACCGTTCATATAGTCCCTACGAGGTTATTAGGATG‑‑TATCACGATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAAT  >  CP043523/1885699‑1885920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑