Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F83 I1 R1 393 530.9 9427126 91.1% 8588111 148.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,885,972 T→A S66S (TCT→TCA)  FTN78_chr1931 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,885,9720TA98.5% 879.5 / ‑0.3 338S66S (TCT→TCA) FTN78_chr1931hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base A (164/168);  total (168/170)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 340 total aligned reads displayed.

GATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAAT  >  CP043523/1885826‑1886116
                                                                                                                                                  |                                                                                                                                                
 aTAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCaaa                                                                                                                                                >  1:1794593/1‑148 (MQ=255)
  tAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAt                                                                                                                                               >  2:3034124/1‑148 (MQ=255)
   agagGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATc                                                                                                                                              >  2:2017968/1‑148 (MQ=255)
   agagGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATc                                                                                                                                              >  2:532207/1‑148 (MQ=255)
    gagGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCa                                                                                                                                             <  1:3031594/148‑1 (MQ=255)
        gatgatgAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgcta                                                                                                                                         >  2:670624/2‑145 (MQ=255)
        gatgatgAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgcta                                                                                                                                         >  1:3663862/2‑145 (MQ=255)
         atgatgAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgctac                                                                                                                                        >  1:4301914/1‑144 (MQ=255)
          tgatgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgctaca                                                                                                                                       >  1:3203267/1‑143 (MQ=255)
            atgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAgctacaaa                                                                                                                                     <  2:71406/148‑8 (MQ=255)
            atgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACaaa                                                                                                                                     <  2:4533235/148‑1 (MQ=255)
             tgaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAt                                                                                                                                    <  1:201007/148‑1 (MQ=255)
              gaAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAtc                                                                                                                                   >  2:4215874/1‑147 (MQ=255)
                aaTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAtcaa                                                                                                                                 >  2:3855520/1‑145 (MQ=255)
                aaTGGGCCGCATTGGGAACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaa                                                                                                                                 <  1:243568/148‑1 (MQ=255)
                 aTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaa                                                                                                                                >  1:1473299/1‑148 (MQ=255)
                 aTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaa                                                                                                                                >  1:1360633/1‑148 (MQ=255)
                 aTGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaa                                                                                                                                >  1:3113949/1‑148 (MQ=255)
                    gggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGtt                                                                                                                             >  2:858818/5‑148 (MQ=255)
                     ggcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTATCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagtta                                                                                                                            <  1:1415097/145‑5 (MQ=255)
                      gcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaa                                                                                                                           >  2:1395826/3‑143 (MQ=255)
                      gcgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaa                                                                                                                           <  1:1433797/146‑6 (MQ=255)
                       cgCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGttaaa                                                                                                                          >  1:1537414/2‑145 (MQ=255)
                        gCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaaat                                                                                                                         <  1:2780743/148‑8 (MQ=255)
                        gCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCaaagttaaat                                                                                                                         <  2:3941984/148‑8 (MQ=255)
                         cATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAata                                                                                                                        >  1:3983045/1‑148 (MQ=255)
                            ttGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCa                                                                                                                     >  1:2913535/1‑148 (MQ=255)
                            ttGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCa                                                                                                                     >  2:555132/1‑148 (MQ=255)
                             tGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaa                                                                                                                    <  2:2920411/148‑1 (MQ=255)
                                 aCTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCaatcaa                                                                                                                <  1:2317594/148‑5 (MQ=255)
                                 aCTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCGCAATCAGTCTTTTTCAAATCAGCTACAAATCAAGGTTAAATATCaatcaa                                                                                                                >  2:4437787/1‑144 (MQ=255)
                                                gaggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATg                                                                                                 <  2:950458/142‑1 (MQ=255)
                                                  ggctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGaa                                                                                               >  1:1699494/5‑148 (MQ=255)
                                                   gctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAg                                                                                              <  2:4069490/145‑1 (MQ=255)
                                                   gctcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAg                                                                                              >  2:885035/4‑148 (MQ=255)
                                                     tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                            >  2:3416312/2‑148 (MQ=255)
                                                     tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                            >  2:4525032/2‑148 (MQ=255)
                                                     tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                            <  1:3542210/147‑1 (MQ=255)
                                                     tcAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTc                                                                                            >  1:2589944/2‑148 (MQ=255)
                                                       aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                                                          <  2:495335/148‑1 (MQ=255)
                                                       aaaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTa                                                                                          <  2:2313519/148‑1 (MQ=255)
                                                        aaaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAt                                                                                         >  1:2075871/1‑148 (MQ=255)
                                                         aaTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATg                                                                                        >  2:1190749/1‑148 (MQ=255)
                                                          aTACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATgg                                                                                       >  1:2280189/1‑148 (MQ=255)
                                                           tACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGt                                                                                      >  1:1871600/1‑148 (MQ=255)
                                                             cTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttt                                                                                    >  1:4145462/1‑146 (MQ=255)
                                                             cTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttt                                                                                    <  1:458571/148‑3 (MQ=255)
                                                               tttGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttttg                                                                                  >  2:1484547/1‑144 (MQ=255)
                                                                ttGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGttttgt                                                                                 >  2:1032865/1‑143 (MQ=255)
                                                                                 gatgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaata                                                                >  2:1970911/4‑148 (MQ=9)
                                                                                   tgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataat                                                              >  1:4556284/2‑148 (MQ=9)
                                                                                   tgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataat                                                              <  1:3469513/147‑1 (MQ=9)
                                                                                   tgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataat                                                              >  2:1787726/2‑148 (MQ=9)
                                                                                   tgataagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCaataat                                                              >  2:960757/2‑148 (MQ=9)
                                                                                      taagaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCaa                                                           <  2:822249/148‑1 (MQ=9)
                                                                                         gaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTa                                                        <  1:4069269/145‑1 (MQ=9)
                                                                                         gaaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTa                                                        <  2:1802738/145‑1 (MQ=9)
                                                                                          aaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTaa                                                       <  2:4285257/146‑1 (MQ=9)
                                                                                          aaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTaa                                                       >  2:48024/3‑148 (MQ=9)
                                                                                          aaaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTaa                                                       <  1:1281045/146‑1 (MQ=9)
                                                                                           aaaTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAag                                                      <  2:1121449/147‑1 (MQ=9)
                                                                                             aTTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAagag                                                    >  1:180807/1‑148 (MQ=9)
                                                                                               tCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGtt                                                  <  1:4692355/148‑1 (MQ=9)
                                                                                                 aaGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaa                                                >  2:4362629/1‑148 (MQ=9)
                                                                                                  aGCATTACCACACCATGTAGTCCCTTGTCTTCTCCATCAGTCTTTTTCAAATCAGCTCCAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTaaa                                               >  2:3250012/1‑148 (MQ=9)
                                                                                                   gctttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAt                                              <  2:3594891/145‑1 (MQ=9)
                                                                                                   gctttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAt                                              <  2:3462887/145‑1 (MQ=9)
                                                                                                     tttACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCa                                            <  1:2737532/147‑1 (MQ=9)
                                                                                                        aCCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACg                                         >  2:145549/1‑148 (MQ=9)
                                                                                                          cacaTCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGa                                       >  2:2874567/1‑148 (MQ=9)
                                                                                                              tcatgTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACACATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGTTaaa                                   <  1:1090854/148‑1 (MQ=9)
                                                                                                                   tagtccTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatcta                              <  2:65531/143‑1 (MQ=12)
                                                                                                                     gtcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatc                            >  1:3777867/8‑148 (MQ=12)
                                                                                                                     gtcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatc                            >  1:746774/8‑148 (MQ=9)
                                                                                                                     gtccTTTATCTTCTCAATCAGTCTTTTTCCAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatc                            <  2:4161714/145‑1 (MQ=12)
                                                                                                                      tcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGACGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatct                           >  1:941289/7‑148 (MQ=17)
                                                                                                                      tcctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAatctatct                           >  2:1657044/7‑148 (MQ=12)
                                                                                                                       cctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTAtctc                          >  1:102600/6‑148 (MQ=14)
                                                                                                                        ctttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCa                         >  2:2852058/5‑148 (MQ=12)
                                                                                                                        cTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCa                         <  2:3768568/148‑1 (MQ=12)
                                                                                                                         tttaTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCat                        <  1:2111934/145‑1 (MQ=17)
                                                                                                                           taTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCatat                      >  1:174873/2‑148 (MQ=12)
                                                                                                                            aTCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATc                     <  1:1315989/148‑1 (MQ=17)
                                                                                                                             tcttctCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATcc                    >  2:2439588/1‑148 (MQ=17)
                                                                                                                                tctcaatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAtt                 >  2:2704041/8‑148 (MQ=18)
                                                                                                                                  tcaatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttga               >  2:4678934/6‑146 (MQ=18)
                                                                                                                                  tcaatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttga               >  2:2596956/6‑146 (MQ=18)
                                                                                                                                   caatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaa              <  1:670624/144‑4 (MQ=18)
                                                                                                                                    aatcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaac             <  2:2189305/145‑5 (MQ=18)
                                                                                                                                      tcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaacaa           <  2:3521361/147‑7 (MQ=18)
                                                                                                                                      tcAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAttgaacaa           <  1:2803405/147‑7 (MQ=18)
                                                                                                                                          tctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAtta       <  2:2573369/145‑3 (MQ=18)
                                                                                                                                          tctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAtta       >  2:3157687/4‑146 (MQ=18)
                                                                                                                                           ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCATAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat      <  2:2389851/146‑4 (MQ=255)
                                                                                                                                           ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat      <  2:1343381/146‑4 (MQ=16)
                                                                                                                                           ctttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttat      <  1:3327189/146‑4 (MQ=16)
                                                                                                                                             ttttCAAATCATCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttataa    <  2:1301654/148‑6 (MQ=18)
                                                                                                                                             ttttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttataa    <  2:3326064/148‑6 (MQ=18)
                                                                                                                                              tttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttataaa   <  2:3663862/148‑7 (MQ=18)
                                                                                                                                              tttCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAAttataaa   <  1:4387449/148‑7 (MQ=16)
                                                                                                                                                  |                                                                                                                                                
GATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAAT  >  CP043523/1885826‑1886116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAATAT  >  CP043523/1885826‑1886118
                                                                                                                                                  |                                                                                                                                                  
GATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAT                                                                                                                                                 >  SRR13214404.1815130/1‑150 (MQ=51)
GATAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAAT                                                                                                                                                 <  SRR13214404.2691135/150‑1 (MQ=51)
  TAGAGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCA                                                                                                                                               <  SRR13214404.1893442/150‑1 (MQ=48)
     AGGGATGATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCT                                                                                                                                            <  SRR13214404.140797/150‑1 (MQ=45)
           GATGAAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAAT                                                                                                                                      <  SRR13214404.555990/150‑1 (MQ=42)
               aaagggggcgcatttgacactggacaactcccggaggctcaaaataatcTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                                                                  <  SRR13214404.2802332/101‑1 (MQ=23)
               AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                                                                  >  SRR13214404.1850991/1‑150 (MQ=36)
               AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                                                                  >  SRR13214404.1483548/1‑150 (MQ=36)
               AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                                                                  >  SRR13214404.1370206/1‑150 (MQ=36)
               AAATGGGGCGCATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAA                                                                                                                                  >  SRR13214404.3133814/1‑150 (MQ=36)
                         cgtttgcgactggcgatctcaagcagactCAAAATATTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATC                                                                                                                        <  SRR13214404.267649/121‑1 (MQ=28)
                         CATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATC                                                                                                                        >  SRR13214404.2309674/1‑150 (MQ=31)
                          ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                                                       <  SRR13214404.813066/150‑1 (MQ=31)
                          ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                                                       <  SRR13214404.813715/150‑1 (MQ=31)
                          ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                                                       >  SRR13214404.2932216/1‑150 (MQ=31)
                          ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                                                       >  SRR13214404.4451203/1‑150 (MQ=31)
                          ATTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCA                                                                                                                       <  SRR13214404.2761202/150‑1 (MQ=31)
                           TTTGCGACTGGAGAACTCACGGAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAA                                                                                                                      <  SRR13214404.2592017/150‑1 (MQ=31)
                                  ctggagaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGAGGATAAGAAAATTCAAGCTTTACCACATCATCTAGTGCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGctacaaatcaaagttaaatatcaatcaaaaa                                                                                                               >  SRR13214404.3943625/21‑119 (MQ=60)
                                   tggagaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGA                                                                                                              >  SRR13214404.1897565/20‑150 (MQ=34)
                                   tggagaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGA                                                                                                              <  SRR13214404.1602666/131‑1 (MQ=34)
                                    ggagaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGAT                                                                                                             <  SRR13214404.854949/132‑1 (MQ=34)
                                      agaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAA                                                                                                           >  SRR13214404.2619346/17‑150 (MQ=33)
                                       gaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAA                                                                                                          >  SRR13214404.1306766/16‑150 (MQ=33)
                                       gaactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAA                                                                                                          >  SRR13214404.68685/16‑150 (MQ=33)
                                        aactcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAAT                                                                                                         <  SRR13214404.4676339/136‑1 (MQ=34)
                                         actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                                                        <  SRR13214404.772582/137‑1 (MQ=36)
                                         actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                                                        <  SRR13214404.314325/137‑1 (MQ=36)
                                         actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                                                        >  SRR13214404.2470066/14‑150 (MQ=36)
                                         actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                                                        <  SRR13214404.3005812/137‑1 (MQ=36)
                                         actcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATT                                                                                                        <  SRR13214404.1347705/137‑1 (MQ=36)
                                          ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                                                       >  SRR13214404.2343004/13‑150 (MQ=37)
                                          ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                                                       <  SRR13214404.4253766/138‑1 (MQ=37)
                                          ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                                                       >  SRR13214404.3089837/13‑150 (MQ=37)
                                          ctcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTA                                                                                                       >  SRR13214404.3090629/13‑150 (MQ=37)
                                           tcacggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAA                                                                                                      <  SRR13214404.3982827/139‑1 (MQ=38)
                                             acggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAAT                                                                                                    >  SRR13214404.3320793/10‑150 (MQ=38)
                                               ggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGA                                                                                                  <  SRR13214404.464430/143‑1 (MQ=38)
                                               ggaggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGA                                                                                                  >  SRR13214404.173551/8‑150 (MQ=38)
                                                GAGGCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAA                                                                                                 >  SRR13214404.1711236/1‑150 (MQ=36)
                                                 aggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAG                                                                                                >  SRR13214404.133840/6‑150 (MQ=39)
                                                 aggctCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAG                                                                                                >  SRR13214404.4257529/6‑150 (MQ=39)
                                                   GCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTC                                                                                              >  SRR13214404.2606769/1‑150 (MQ=41)
                                                   GCTCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTC                                                                                              >  SRR13214404.3650790/1‑150 (MQ=41)
                                                     TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                                                            >  SRR13214404.4607660/1‑150 (MQ=44)
                                                     TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                                                            >  SRR13214404.1674855/1‑150 (MQ=44)
                                                     TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                                                            <  SRR13214404.3788542/150‑1 (MQ=44)
                                                     TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                                                            <  SRR13214404.3564777/150‑1 (MQ=44)
                                                     TCAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTA                                                                                            >  SRR13214404.1092920/1‑150 (MQ=44)
                                                      CAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAT                                                                                           >  SRR13214404.4639224/1‑150 (MQ=45)
                                                      CAAAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTAT                                                                                           >  SRR13214404.2089862/1‑150 (MQ=45)
                                                        AAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGG                                                                                         >  SRR13214404.2295313/1‑150 (MQ=45)
                                                        AAATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGG                                                                                         <  SRR13214404.4695986/150‑1 (MQ=45)
                                                         AATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGT                                                                                        >  SRR13214404.1884403/1‑150 (MQ=45)
                                                          ATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAatgcagtcaatggcc                                                                                       >  SRR13214404.4028529/1‑135 (MQ=33)
                                                          ATACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTT                                                                                       >  SRR13214404.4408080/1‑150 (MQ=44)
                                                           TACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtt                                                                                      >  SRR13214404.4171535/1‑148 (MQ=43)
                                                           TACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtt                                                                                      >  SRR13214404.1828689/1‑148 (MQ=43)
                                                            ACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTttt                                                                                     <  SRR13214404.4605558/150‑4 (MQ=41)
                                                            ACTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTttt                                                                                     >  SRR13214404.1318287/1‑147 (MQ=41)
                                                             CTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtttg                                                                                    >  SRR13214404.1537117/1‑146 (MQ=40)
                                                             CTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtttg                                                                                    <  SRR13214404.1441529/150‑5 (MQ=40)
                                                             CTTTTGAAAAAGAAAAAAAAGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTtttg                                                                                    <  SRR13214404.461769/150‑5 (MQ=40)
                                                                                AGATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAA                                                                 <  SRR13214404.4097042/150‑1 (MQ=12)
                                                                                 GATGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAAT                                                                >  SRR13214404.4584737/1‑150 (MQ=12)
                                                                                   TGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCA                                                              <  SRR13214404.3491650/150‑1 (MQ=12)
                                                                                   TGATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCA                                                              >  SRR13214404.1831760/1‑150 (MQ=12)
                                                                                    GATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAA                                                             >  SRR13214404.1440149/1‑150 (MQ=12)
                                                                                     ATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAG                                                            >  SRR13214404.4091704/1‑150 (MQ=11)
                                                                                     ATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAG                                                            >  SRR13214404.1378960/1‑150 (MQ=11)
                                                                                     ATAAGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAG                                                            >  SRR13214404.3427999/1‑150 (MQ=11)
                                                                                        AGAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAA                                                         >  SRR13214404.4506080/1‑150 (MQ=11)
                                                                                         GAAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAG                                                        <  SRR13214404.4094922/150‑1 (MQ=12)
                                                                                          AAAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGA                                                       <  SRR13214404.1290084/150‑1 (MQ=14)
                                                                                           AAATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAG                                                      >  SRR13214404.182156/1‑150 (MQ=15)
                                                                                            AATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGT                                                     <  SRR13214404.3740036/150‑1 (MQ=16)
                                                                                             ATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTT                                                    <  SRR13214404.3044395/150‑1 (MQ=16)
                                                                                             ATTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTT                                                    <  SRR13214404.1405613/150‑1 (MQ=16)
                                                                                              TTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTA                                                   >  SRR13214404.3060689/1‑150 (MQ=16)
                                                                                              TTCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTA                                                   <  SRR13214404.1728860/150‑1 (MQ=16)
                                                                                               TCAAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAA                                                  <  SRR13214404.4721638/150‑1 (MQ=16)
                                                                                                 AAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAT                                                >  SRR13214404.2280999/1‑150 (MQ=16)
                                                                                                 AAGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAAT                                                <  SRR13214404.203593/150‑1 (MQ=16)
                                                                                                  AGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATC                                               <  SRR13214404.3242940/150‑1 (MQ=18)
                                                                                                  AGCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATC                                               <  SRR13214404.1288322/150‑1 (MQ=18)
                                                                                                   GCTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCA                                              <  SRR13214404.3501532/150‑1 (MQ=19)
                                                                                                    CTTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAA                                             >  SRR13214404.1320393/1‑150 (MQ=20)
                                                                                                     TTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAAC                                            <  SRR13214404.2755181/150‑1 (MQ=22)
                                                                                                     TTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAAC                                            <  SRR13214404.2756362/150‑1 (MQ=22)
                                                                                                     TTTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAAC                                            <  SRR13214404.4326597/150‑1 (MQ=22)
                                                                                                      TTACCACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACG                                           <  SRR13214404.1752795/150‑1 (MQ=23)
                                                                                                          CACATCATGTAGTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATA                                       <  SRR13214404.969486/150‑1 (MQ=26)
                                                                                                                     GTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTC                            >  SRR13214404.103358/1‑150 (MQ=43)
                                                                                                                     GTCCTTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTC                            <  SRR13214404.2097836/150‑1 (MQ=43)
                                                                                                                         TTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATAT                        >  SRR13214404.176180/1‑150 (MQ=50)
                                                                                                                         TTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATAT                        <  SRR13214404.2813340/150‑1 (MQ=50)
                                                                                                                         TTTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATAT                        <  SRR13214404.2126149/150‑1 (MQ=50)
                                                                                                                          TTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATC                       <  SRR13214404.1459703/150‑1 (MQ=52)
                                                                                                                          TTATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATC                       >  SRR13214404.943894/1‑150 (MQ=52)
                                                                                                                           TATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCC                      >  SRR13214404.1937061/1‑150 (MQ=54)
                                                                                                                            ATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCA                     <  SRR13214404.1325226/150‑1 (MQ=55)
                                                                                                                            ATCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCA                     <  SRR13214404.1325274/150‑1 (MQ=55)
                                                                                                                             TCTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCAT                    <  SRR13214404.1478493/150‑1 (MQ=59)
                                                                                                                              CTTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATT                   <  SRR13214404.1488092/150‑1 (MQ=60)
                                                                                                                               TTCTCAATCAGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTG                  >  SRR13214404.3550908/1‑150 (MQ=60)
                                                                                                                                        AGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTgaacaaatta         >  SRR13214404.3218945/1‑140 (MQ=60)
                                                                                                                                        AGTCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTgaacaaatta         >  SRR13214404.3358615/1‑140 (MQ=60)
                                                                                                                                          TCTTTTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTgaacaaattata       <  SRR13214404.3539602/150‑13 (MQ=60)
                                                                                                                                              TTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAATA   <  SRR13214404.4414952/150‑1 (MQ=60)
                                                                                                                                              TTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAATA   <  SRR13214404.10001/150‑1 (MQ=60)
                                                                                                                                              TTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAATA   <  SRR13214404.2429117/150‑1 (MQ=60)
                                                                                                                                              TTTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAATA   >  SRR13214404.384137/1‑150 (MQ=60)
                                                                                                                                               TTCAAATCAGCTACAAATCAAAGTTAAATATCAATCAAAAGATAAATTAAATGAAGTCTATGGTTTTGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTGAACAAATTATAAATAT  <  SRR13214404.661680/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                  
GATAGAGGAATGATGAAATGGGGGGCATTTGCGACTGGGGAATTAACTGAAGCCCAAAATACTTTTGAAAAAGAAAAAAAAGAAGATAAGATAATTCAAGCATTACCACATCATCTCGTGCTTCATCTTCTTAACCAGTCCTTTTCTAATCAGGTGCAAATTAAAATTCAATATCAAGCCAAAGATAAATTAACTGAAGTCTATGGTAATGTATCAGAATTTATCAATAATCAAGTAAGAGTTAAATCAACGGATAAAATCTATCTCATATCCATTAAGCAAATCGTAAATAT  >  CP043523/1885826‑1886118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑