Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F250 I1 R1 466 525.0 9216842 90.5% 8341242 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP043523 1,848,409 A→T T40S (ACA→TCA)  FTN78_chr1898 → Cold shock protein of CSP family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0435231,848,4090AT99.3% 709.5 / NA 291T40S (ACA→TCA) FTN78_chr1898Cold shock protein of CSP family
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base T (137/151);  total (137/154)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

GAAATAAATATTATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCTCAGCAATCCAATCTGATGGATTCAAAACACTTGATGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAGCTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAA  >  CP043523/1848280‑1848555
                                                                                                                                 |                                                                                                                                                  
                  aaTGGAACAGTAAAATGGTTTAAAGCAGATAAAGGATTTGGCTTTATAACTTCAGAAGAAGGAAAAGATTTGTTCGCTCACTTCTAAGAAATCAAATCTGATGGATTCAAAAAAAATAATGAAAATAAAAAAGTTGAATTTGATGTTgaa                                                                                                              <  2:2630883/150‑1 (MQ=11)
                                          gctactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCa                                                                                      <  1:685339/145‑1 (MQ=11)
                                          gctactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCa                                                                                      >  1:1488145/6‑150 (MQ=11)
                                           ctactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAg                                                                                     <  1:1779661/146‑1 (MQ=11)
                                           ctactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAg                                                                                     <  2:3659452/146‑1 (MQ=11)
                                           ctactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAg                                                                                     >  2:3694693/5‑150 (MQ=11)
                                            tactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGt                                                                                    <  1:2780538/147‑1 (MQ=11)
                                            tactAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGt                                                                                    >  1:223541/4‑150 (MQ=11)
                                             actAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGtt                                                                                   >  2:2402706/3‑150 (MQ=11)
                                             actAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGtt                                                                                   >  2:4456208/3‑150 (MQ=11)
                                             actAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGtt                                                                                   >  1:2934640/3‑150 (MQ=11)
                                              ctAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTa                                                                                  >  1:1374286/2‑150 (MQ=11)
                                              ctAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTa                                                                                  <  2:3809112/149‑1 (MQ=11)
                                              ctAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTGGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTa                                                                                  <  1:4179824/149‑1 (MQ=11)
                                               tAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACGTCAAGCAGTTaa                                                                                 >  1:3385310/1‑150 (MQ=11)
                                                aaaGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAat                                                                                >  1:112656/1‑150 (MQ=11)
                                                 aaGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAata                                                                               <  1:4295978/150‑1 (MQ=11)
                                                 aaGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAata                                                                               <  2:2264538/150‑1 (MQ=11)
                                                  aGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAatat                                                                              >  1:2875145/1‑150 (MQ=11)
                                                  aGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAatat                                                                              >  1:4013613/1‑150 (MQ=11)
                                                     aTTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATcac                                                                           <  1:1046277/150‑1 (MQ=11)
                                                     aTTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATcac                                                                           >  1:492590/1‑150 (MQ=11)
                                                          gttttATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAg                                                                      <  2:2002837/148‑1 (MQ=11)
                                                            tttATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCa                                                                    <  1:3569472/150‑1 (MQ=11)
                                                             ttattaCCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCAt                                                                   >  1:526567/1‑150 (MQ=11)
                                                                           gatggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCACAAAGTTGAAGTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGaaa                                                     <  2:4216863/143‑1 (MQ=14)
                                                                           gatggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGaaa                                                     >  1:3000273/8‑150 (MQ=14)
                                                                           gatggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGaaa                                                     <  1:3143518/143‑1 (MQ=14)
                                                                           gatggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGaaa                                                     <  1:864123/143‑1 (MQ=14)
                                                                             tggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAAt                                                   <  1:2624418/145‑1 (MQ=14)
                                                                              ggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATc                                                  >  2:1124176/5‑150 (MQ=14)
                                                                              ggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATc                                                  <  2:136886/146‑1 (MQ=14)
                                                                              ggtcaaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATc                                                  <  1:1056496/146‑1 (MQ=14)
                                                                                 caaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTg                                               <  1:2415529/149‑1 (MQ=255)
                                                                                 caaGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTg                                               <  1:2103984/149‑1 (MQ=255)
                                                                                     aTTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCaaa                                           <  1:967158/150‑1 (MQ=255)
                                                                                      tttGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCaaaa                                          >  1:1293173/1‑150 (MQ=255)
                                                                                       ttGTTCGCTCACTTCTCATCAATACAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGAaaaaa                                         <  2:1630965/150‑2 (MQ=14)
                                                                                          ttCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTg                                      <  1:1551902/150‑1 (MQ=14)
                                                                                           tCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTgg                                     >  2:3295019/1‑150 (MQ=255)
                                                                                            cGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGa                                    <  1:2349879/150‑1 (MQ=255)
                                                                                            cGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGa                                    <  1:257850/150‑1 (MQ=14)
                                                                                            cGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGa                                    >  1:197909/1‑150 (MQ=14)
                                                                                              cTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGt                                  >  1:1934713/1‑150 (MQ=14)
                                                                                              cTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGt                                  <  1:644541/150‑1 (MQ=14)
                                                                                              cTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGt                                  >  1:1615778/1‑150 (MQ=14)
                                                                                               tCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTc                                 >  2:3432492/1‑150 (MQ=14)
                                                                                               tCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTc                                 >  1:2563773/1‑150 (MQ=14)
                                                                                               tCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTc                                 >  1:1142866/1‑150 (MQ=14)
                                                                                                cACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCa                                >  1:4504761/1‑150 (MQ=14)
                                                                                                 aCTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCAt                               <  1:3117492/150‑1 (MQ=14)
                                                                                                 aCTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCAt                               >  1:2322506/1‑150 (MQ=14)
                                                                                                 aCTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCAt                               <  1:2452584/150‑1 (MQ=14)
                                                                                                  cTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATg                              >  2:2810786/1‑150 (MQ=14)
                                                                                                  cTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATg                              >  2:2806945/1‑150 (MQ=14)
                                                                                                   ttCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGa                             >  1:1076327/1‑150 (MQ=14)
                                                                                                    tctcATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGAc                            >  1:4589398/1‑150 (MQ=17)
                                                                                                    tctcATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGAc                            >  2:60103/1‑150 (MQ=17)
                                                                                                       catcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTc                         >  2:1868580/4‑150 (MQ=17)
                                                                                                       catcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTc                         >  2:3724401/4‑150 (MQ=17)
                                                                                                       catcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTAAAAAAGTTGAATTTGATGTTGAAGAAGGTCACCGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGAAAAAACTGGAGTCATGACTTc                         <  2:2579700/147‑1 (MQ=17)
                                                                                                        atcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCa                        <  1:1671943/148‑1 (MQ=255)
                                                                                                         tcaatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGATGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAAAAAAAAAAAAACTGGAGTCATGACTTCAg                       <  2:3779793/149‑1 (MQ=14)
                                                                                                           aatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGtt                     <  1:251932/150‑1 (MQ=255)
                                                                                                           aatccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGtt                     >  2:4172426/1‑150 (MQ=255)
                                                                                                            atccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttt                    <  1:372113/150‑1 (MQ=255)
                                                                                                            atccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttt                    >  2:645677/1‑150 (MQ=255)
                                                                                                            atccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttt                    <  1:372597/150‑1 (MQ=255)
                                                                                                            atccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttt                    >  1:933489/1‑150 (MQ=255)
                                                                                                              ccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttttt                  >  2:4403662/1‑150 (MQ=255)
                                                                                                              ccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttttt                  <  2:4015893/150‑1 (MQ=255)
                                                                                                              ccaatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttttt                  <  1:3300954/150‑1 (MQ=255)
                                                                                                               caatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGtttttt                 >  1:2093839/1‑150 (MQ=255)
                                                                                                               caatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGtttttt                 >  2:3061038/1‑150 (MQ=255)
                                                                                                               caatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGtttttt                 >  1:2659811/1‑150 (MQ=255)
                                                                                                                aatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttttttt                >  2:2217497/1‑150 (MQ=255)
                                                                                                                aatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttttttt                <  2:3596243/150‑1 (MQ=255)
                                                                                                                aatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttttttt                >  2:3188740/1‑150 (MQ=255)
                                                                                                                aatcTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGttttttt                <  2:145415/150‑1 (MQ=255)
                                                                                                                   ctcattgATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGCCAACGTGGCCCTCAAGCAGTTAATATCACAAAAGCATAATTGTCTCAGACAATCGTGCAAAAACTCGAGTCAGGACTCCAGTTTTTTtata             >  2:1831907/7‑150 (MQ=14)
                                                                                                                    tGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTatat            >  2:816832/1‑150 (MQ=255)
                                                                                                                    tGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTatat            <  1:191472/150‑1 (MQ=255)
                                                                                                                    tGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTAATGACTTGAGTTTTTTTatat            >  1:376717/1‑150 (MQ=17)
                                                                                                                     gATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATAtt           <  1:3495511/150‑1 (MQ=255)
                                                                                                                     gATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATAtt           <  2:2184288/150‑1 (MQ=255)
                                                                                                                     gATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATAtt           >  1:3912595/1‑150 (MQ=255)
                                                                                                                      aTGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTc          >  1:760908/1‑150 (MQ=255)
                                                                                                                        ggATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAg        >  2:3458858/1‑150 (MQ=255)
                                                                                                                        ggATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAg        >  1:2017344/1‑150 (MQ=255)
                                                                                                                        ggATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAg        <  1:3067388/150‑1 (MQ=255)
                                                                                                                        ggATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAg        >  2:432629/1‑150 (MQ=255)
                                                                                                                          aTTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGtt      <  1:2438704/150‑1 (MQ=255)
                                                                                                                           ttCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTg     <  2:4579778/150‑1 (MQ=255)
                                                                                                                            tCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGc    >  2:2107846/1‑150 (MQ=255)
                                                                                                                            tCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGc    <  2:1325229/150‑1 (MQ=255)
                                                                                                                            tCAAATCACTTGACGAAGGTCAAAAAGTTGAATTCGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCaatgtc    <  2:4480634/150‑6 (MQ=18)
                                                                                                                            tCAAAGCACTTGACGAAGGTAAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGc    <  2:1589374/150‑1 (MQ=255)
                                                                                                                             cAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCa   <  2:2742775/150‑1 (MQ=255)
                                                                                                                              aaatCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCaa  >  2:918876/1‑150 (MQ=255)
                                                                                                                              aaatCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCaa  >  1:4428286/1‑150 (MQ=255)
                                                                                                                                 |                                                                                                                                                  
GAAATAAATATTATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCTCAGCAATCCAATCTGATGGATTCAAAACACTTGATGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAGCTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAA  >  CP043523/1848280‑1848555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 212 total aligned reads displayed.
AGGAAATAA‑‑ATATTATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCTCAGCAATCCAATCTGATGGATTCAAAACACTTGATGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAGCTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAATGA  >  CP043523/1848278‑1848558
                                                                                                                                     |                                                                                                                                                     
AGGAAATAAATATATTATGGCAAATGGAACAGTAAAATGGTTTAACGCTACTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAAGCACTTGGCGAAGGTCA                                                                                                                                       >  SRR13214403.3694811/1‑150 (MQ=0)
                                          taacgctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCA                                                                                             >  SRR13214403.244273/45‑150 (MQ=0)
                                          taacgctactaaaggatttggttttattacctctgaagacggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCA                                                                                             <  SRR13214403.4506641/106‑1 (MQ=0)
                                            acgctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAG                                                                                           <  SRR13214403.967080/108‑1 (MQ=4)
                                            acgctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAG                                                                                           <  SRR13214403.4627034/108‑1 (MQ=4)
                                            acgctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAG                                                                                           <  SRR13214403.4627135/108‑1 (MQ=4)
                                             cgctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGC                                                                                          <  SRR13214403.1712926/109‑1 (MQ=6)
                                              gctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCA                                                                                         >  SRR13214403.3040696/41‑150 (MQ=8)
                                              gctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCA                                                                                         >  SRR13214403.4227825/41‑150 (MQ=8)
                                              gctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCA                                                                                         >  SRR13214403.1495115/41‑150 (MQ=8)
                                              gctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCA                                                                                         <  SRR13214403.689022/110‑1 (MQ=8)
                                              gctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCA                                                                                         >  SRR13214403.2728862/41‑150 (MQ=8)
                                              gctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCGCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCA                                                                                         >  SRR13214403.2978536/41‑150 (MQ=8)
                                               ctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAG                                                                                        <  SRR13214403.1143513/111‑1 (MQ=8)
                                               ctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAG                                                                                        <  SRR13214403.1143514/111‑1 (MQ=8)
                                               ctactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAG                                                                                        <  SRR13214403.1787939/111‑1 (MQ=8)
                                                tactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGT                                                                                       <  SRR13214403.2877643/112‑1 (MQ=8)
                                                tactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGT                                                                                       >  SRR13214403.224824/39‑150 (MQ=8)
                                                tactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGT                                                                                       <  SRR13214403.2877762/112‑1 (MQ=8)
                                                tactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGT                                                                                       >  SRR13214403.522635/39‑150 (MQ=8)
                                                tactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGT                                                                                       >  SRR13214403.3652269/39‑150 (MQ=8)
                                                tactaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGT                                                                                       <  SRR13214403.2793092/112‑1 (MQ=8)
                                                 actaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTT                                                                                      >  SRR13214403.2947818/38‑150 (MQ=10)
                                                 actaaaggatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTT                                                                                      >  SRR13214403.2947816/38‑150 (MQ=10)
                                                  CTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTA                                                                                     >  SRR13214403.1380818/1‑150 (MQ=8)
                                                  CTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTA                                                                                     <  SRR13214403.2061238/150‑1 (MQ=8)
                                                  CTAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTGGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTA                                                                                     <  SRR13214403.4200250/150‑1 (MQ=8)
                                                   TAAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACGTCAAGCAGTTAA                                                                                    >  SRR13214403.3400442/1‑150 (MQ=2)
                                                    AAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAAT                                                                                   >  SRR13214403.895704/1‑150 (MQ=15)
                                                    AAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAAT                                                                                   >  SRR13214403.113285/1‑150 (MQ=15)
                                                    AAAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAAT                                                                                   <  SRR13214403.3216210/150‑1 (MQ=15)
                                                     AAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATA                                                                                  <  SRR13214403.3259546/150‑1 (MQ=17)
                                                     AAGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATA                                                                                  <  SRR13214403.4317265/150‑1 (MQ=17)
                                                      AGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATAT                                                                                 >  SRR13214403.2888073/1‑150 (MQ=17)
                                                      AGGATTTGGTTTTATTACCTCTGAAGATGGTCAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATAT                                                                                 >  SRR13214403.4032702/1‑150 (MQ=17)
                                                        gatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCA                                                                               <  SRR13214403.1352552/120‑1 (MQ=21)
                                                        gatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCA                                                                               <  SRR13214403.1349558/120‑1 (MQ=21)
                                                        gatttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCA                                                                               <  SRR13214403.3339842/120‑1 (MQ=21)
                                                         atttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCAC                                                                              <  SRR13214403.2463012/121‑1 (MQ=21)
                                                         atttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCAC                                                                              <  SRR13214403.1051462/121‑1 (MQ=21)
                                                         atttggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCAC                                                                              >  SRR13214403.495267/30‑150 (MQ=21)
                                                            tggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAA                                                                           <  SRR13214403.719869/124‑1 (MQ=21)
                                                            tggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAA                                                                           <  SRR13214403.106668/124‑1 (MQ=21)
                                                            tggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAA                                                                           <  SRR13214403.896755/124‑1 (MQ=21)
                                                             ggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAA                                                                          >  SRR13214403.21816/26‑150 (MQ=21)
                                                             ggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAA                                                                          <  SRR13214403.2079542/125‑1 (MQ=21)
                                                             ggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAA                                                                          <  SRR13214403.3899332/125‑1 (MQ=21)
                                                             ggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAA                                                                          <  SRR13214403.4301668/125‑1 (MQ=21)
                                                             ggttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAA                                                                          <  SRR13214403.249798/125‑1 (MQ=21)
                                                              gttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAG                                                                         <  SRR13214403.463244/126‑1 (MQ=21)
                                                              gttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAG                                                                         >  SRR13214403.1338701/25‑150 (MQ=21)
                                                              gttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAG                                                                         <  SRR13214403.1640623/126‑1 (MQ=21)
                                                              gttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAG                                                                         <  SRR13214403.1627054/126‑1 (MQ=21)
                                                               ttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGC                                                                        <  SRR13214403.2807401/127‑1 (MQ=21)
                                                               ttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGC                                                                        <  SRR13214403.2457520/127‑1 (MQ=21)
                                                               ttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGC                                                                        >  SRR13214403.3274458/24‑150 (MQ=21)
                                                               ttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGC                                                                        <  SRR13214403.1118161/127‑1 (MQ=21)
                                                               ttttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGC                                                                        <  SRR13214403.1121873/127‑1 (MQ=21)
                                                                tttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCA                                                                       <  SRR13214403.3585638/128‑1 (MQ=21)
                                                                tttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCA                                                                       <  SRR13214403.138570/128‑1 (MQ=21)
                                                                 ttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCAT                                                                      >  SRR13214403.529416/22‑150 (MQ=21)
                                                                 ttattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCAT                                                                      <  SRR13214403.1079959/129‑1 (MQ=21)
                                                                  tattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATA                                                                     >  SRR13214403.2074479/21‑150 (MQ=21)
                                                                  tattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATA                                                                     >  SRR13214403.534240/21‑150 (MQ=21)
                                                                  tattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATA                                                                     <  SRR13214403.1442325/130‑1 (MQ=21)
                                                                  tattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATA                                                                     <  SRR13214403.1458505/130‑1 (MQ=21)
                                                                  tattacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATA                                                                     >  SRR13214403.3044054/21‑150 (MQ=21)
                                                                    ttacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAAT                                                                   >  SRR13214403.1539589/19‑150 (MQ=21)
                                                                     tacctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATT                                                                  <  SRR13214403.3860584/133‑1 (MQ=21)
                                                                       cctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATGTCACAAAAGCATAATTGT                                                                >  SRR13214403.3808113/16‑150 (MQ=24)
                                                                       cctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGT                                                                <  SRR13214403.3328701/135‑1 (MQ=25)
                                                                       cctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGT                                                                <  SRR13214403.294012/135‑1 (MQ=25)
                                                                        ctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTA                                                               <  SRR13214403.3774791/136‑1 (MQ=27)
                                                                        ctctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTA                                                               >  SRR13214403.4048049/15‑150 (MQ=27)
                                                                         tctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTAT                                                              <  SRR13214403.2347689/137‑1 (MQ=29)
                                                                         tctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTAT                                                              >  SRR13214403.3340819/14‑150 (MQ=29)
                                                                         tctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTAT                                                              >  SRR13214403.2023820/14‑150 (MQ=29)
                                                                         tctgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTAT                                                              >  SRR13214403.2023639/14‑150 (MQ=29)
                                                                           tgaagatggtcAAGATTTGTTCGCTCACTTCTCATCTATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCT                                                            <  SRR13214403.3784389/139‑1 (MQ=32)
                                                                           tgaagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCT                                                            >  SRR13214403.1105065/12‑150 (MQ=33)
                                                                             aagatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGA                                                          >  SRR13214403.2535347/10‑150 (MQ=37)
                                                                              agatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAA                                                         >  SRR13214403.3740055/9‑150 (MQ=38)
                                                                               gatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAA                                                        <  SRR13214403.3157589/143‑1 (MQ=40)
                                                                               gatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAA                                                        <  SRR13214403.868544/143‑1 (MQ=40)
                                                                               gatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAA                                                        >  SRR13214403.3013735/8‑150 (MQ=40)
                                                                               gatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAA                                                        >  SRR13214403.3016769/8‑150 (MQ=40)
                                                                               gatggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAA                                                        <  SRR13214403.3156992/143‑1 (MQ=40)
                                                                                 tggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAAT                                                      <  SRR13214403.4525727/145‑1 (MQ=40)
                                                                                 tggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAAT                                                      <  SRR13214403.2636424/145‑1 (MQ=40)
                                                                                 tggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAAT                                                      <  SRR13214403.2636383/145‑1 (MQ=40)
                                                                                  ggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATC                                                     <  SRR13214403.1061346/146‑1 (MQ=40)
                                                                                  ggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATC                                                     <  SRR13214403.1061729/146‑1 (MQ=40)
                                                                                  ggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATC                                                     <  SRR13214403.1433124/146‑1 (MQ=40)
                                                                                  ggtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATC                                                     <  SRR13214403.2277585/146‑1 (MQ=40)
                                                                                  ggtcAAGATTTGGTCGCTCCCTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAATAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATC                                                     <  SRR13214403.1065374/146‑1 (MQ=37)
                                                                                   gtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACACAAGCATAATTGTATCTGAAAATCG                                                    >  SRR13214403.4003650/4‑150 (MQ=39)
                                                                                   gtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACACAAGCATAATTGTATCTGAAAATCG                                                    >  SRR13214403.4000191/4‑150 (MQ=39)
                                                                                   gtcAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCG                                                    >  SRR13214403.3590900/4‑150 (MQ=40)
                                                                                     CAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTG                                                  <  SRR13214403.2113644/150‑1 (MQ=40)
                                                                                     CAAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTG                                                  <  SRR13214403.2426564/150‑1 (MQ=40)
                                                                                      AAGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGC                                                 <  SRR13214403.3117459/150‑1 (MQ=40)
                                                                                       AGATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCA                                                <  SRR13214403.1928953/150‑1 (MQ=40)
                                                                                        GATTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAA                                               >  SRR13214403.1228230/1‑150 (MQ=42)
                                                                                          TTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAA                                             >  SRR13214403.2507062/1‑150 (MQ=46)
                                                                                          TTTGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAA                                             >  SRR13214403.1299344/1‑150 (MQ=46)
                                                                                            TGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAAC                                           <  SRR13214403.2346338/150‑1 (MQ=45)
                                                                                            TGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAAC                                           >  SRR13214403.2068955/1‑150 (MQ=45)
                                                                                            TGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAAC                                           <  SRR13214403.2342877/150‑1 (MQ=45)
                                                                                            TGTTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAAC                                           <  SRR13214403.1086542/150‑1 (MQ=45)
                                                                                              TTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTG                                         <  SRR13214403.183360/150‑1 (MQ=41)
                                                                                              TTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTG                                         <  SRR13214403.1559130/150‑1 (MQ=41)
                                                                                              TTCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTG                                         <  SRR13214403.942174/150‑1 (MQ=41)
                                                                                               TCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGG                                        <  SRR13214403.2897056/150‑1 (MQ=39)
                                                                                               TCGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGG                                        <  SRR13214403.1746358/150‑1 (MQ=39)
                                                                                                CGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGA                                       >  SRR13214403.197813/1‑150 (MQ=39)
                                                                                                CGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGA                                       <  SRR13214403.259303/150‑1 (MQ=39)
                                                                                                CGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGA                                       <  SRR13214403.2360645/150‑1 (MQ=39)
                                                                                                CGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGA                                       <  SRR13214403.2360711/150‑1 (MQ=39)
                                                                                                CGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGA                                       >  SRR13214403.238177/1‑150 (MQ=39)
                                                                                                CGCTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGA                                       >  SRR13214403.664262/1‑150 (MQ=39)
                                                                                                  CTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGANT                                     <  SRR13214403.1841840/150‑1 (MQ=38)
                                                                                                  CTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGT                                     >  SRR13214403.1943662/1‑150 (MQ=41)
                                                                                                  CTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGT                                     >  SRR13214403.1623304/1‑150 (MQ=41)
                                                                                                  CTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGT                                     <  SRR13214403.649516/150‑1 (MQ=41)
                                                                                                  CTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGT                                     >  SRR13214403.1623710/1‑150 (MQ=41)
                                                                                                  CTCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGT                                     >  SRR13214403.389294/1‑150 (MQ=41)
                                                                                                   TCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTC                                    >  SRR13214403.1163959/1‑150 (MQ=43)
                                                                                                   TCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTC                                    >  SRR13214403.4325185/1‑150 (MQ=43)
                                                                                                   TCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTC                                    >  SRR13214403.3742829/1‑150 (MQ=43)
                                                                                                   TCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTC                                    >  SRR13214403.2575466/1‑150 (MQ=43)
                                                                                                   TCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTC                                    >  SRR13214403.1148473/1‑150 (MQ=43)
                                                                                                   TCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTC                                    >  SRR13214403.61853/1‑150 (MQ=43)
                                                                                                   TCACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCCCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTC                                    >  SRR13214403.3742122/1‑150 (MQ=41)
                                                                                                    CACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCA                                   >  SRR13214403.2112054/1‑150 (MQ=45)
                                                                                                    CACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCA                                   >  SRR13214403.2168868/1‑150 (MQ=45)
                                                                                                    CACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCA                                   >  SRR13214403.2168725/1‑150 (MQ=45)
                                                                                                    CACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCA                                   >  SRR13214403.4528834/1‑150 (MQ=45)
                                                                                                    CACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCA                                   <  SRR13214403.2693145/150‑1 (MQ=45)
                                                                                                    CACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCA                                   >  SRR13214403.3318810/1‑150 (MQ=45)
                                                                                                     ACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCAT                                  <  SRR13214403.3131470/150‑1 (MQ=46)
                                                                                                     ACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCAT                                  <  SRR13214403.854484/150‑1 (MQ=46)
                                                                                                     ACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCAT                                  <  SRR13214403.2463814/150‑1 (MQ=46)
                                                                                                     ACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCAT                                  <  SRR13214403.3131475/150‑1 (MQ=46)
                                                                                                     ACTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCAT                                  >  SRR13214403.2333162/1‑150 (MQ=46)
                                                                                                      CTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAGTCGTGCAAAAACTGGAGTCATG                                 >  SRR13214403.3433410/1‑150 (MQ=47)
                                                                                                      CTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATG                                 >  SRR13214403.452891/1‑150 (MQ=48)
                                                                                                      CTTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATG                                 <  SRR13214403.4214219/150‑1 (MQ=48)
                                                                                                       TTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGA                                >  SRR13214403.1081641/1‑150 (MQ=50)
                                                                                                       TTCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGA                                <  SRR13214403.2450304/150‑1 (MQ=50)
                                                                                                        TCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGAC                               <  SRR13214403.2611574/150‑1 (MQ=52)
                                                                                                        TCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGAC                               >  SRR13214403.4613859/1‑150 (MQ=52)
                                                                                                        TCTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGAC                               >  SRR13214403.4613884/1‑150 (MQ=52)
                                                                                                         CTCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACT                              <  SRR13214403.2891308/150‑1 (MQ=54)
                                                                                                          TCATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTT                             <  SRR13214403.74535/150‑1 (MQ=56)
                                                                                                           CATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTC                            <  SRR13214403.146726/150‑1 (MQ=59)
                                                                                                            ATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCA                           >  SRR13214403.2631379/1‑150 (MQ=60)
                                                                                                            ATCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCA                           <  SRR13214403.1679745/150‑1 (MQ=60)
                                                                                                             TCAATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAG                          <  SRR13214403.3459771/150‑1 (MQ=60)
                                                                                                               AATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTT                        <  SRR13214403.2580133/150‑1 (MQ=60)
                                                                                                               AATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTT                        <  SRR13214403.2579492/150‑1 (MQ=60)
                                                                                                               AATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTT                        >  SRR13214403.2435292/1‑150 (MQ=60)
                                                                                                               AATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTT                        <  SRR13214403.2666019/150‑1 (MQ=60)
                                                                                                               AATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTT                        >  SRR13214403.2000222/1‑150 (MQ=60)
                                                                                                               AATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTT                        <  SRR13214403.253355/150‑1 (MQ=60)
                                                                                                                ATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTT                       <  SRR13214403.374682/150‑1 (MQ=60)
                                                                                                                ATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTT                       <  SRR13214403.374197/150‑1 (MQ=60)
                                                                                                                ATCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTT                       >  SRR13214403.938187/1‑150 (MQ=60)
                                                                                                                 TCCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTT                      <  SRR13214403.3585160/150‑1 (MQ=60)
                                                                                                                  CCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTT                     <  SRR13214403.3762709/150‑1 (MQ=60)
                                                                                                                  CCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTT                     <  SRR13214403.3315715/150‑1 (MQ=60)
                                                                                                                  CCAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTT                     <  SRR13214403.4545981/150‑1 (MQ=60)
                                                                                                                   CAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTT                    >  SRR13214403.2671903/1‑150 (MQ=60)
                                                                                                                   CAATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTT                    >  SRR13214403.2103446/1‑150 (MQ=60)
                                                                                                                    AATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTT                   <  SRR13214403.194241/150‑1 (MQ=60)
                                                                                                                     ATCTGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTA                  <  SRR13214403.1064595/150‑1 (MQ=60)
                                                                                                                        TGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATAT               <  SRR13214403.192584/150‑1 (MQ=60)
                                                                                                                        TGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATAT               >  SRR13214403.1584140/1‑150 (MQ=60)
                                                                                                                        TGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATAT               >  SRR13214403.855032/1‑150 (MQ=60)
                                                                                                                        TGATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTAATGACTTGAGTTTTTTTATAT               >  SRR13214403.378818/1‑150 (MQ=60)
                                                                                                                         GATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATT              >  SRR13214403.3931161/1‑150 (MQ=60)
                                                                                                                         GATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATT              <  SRR13214403.2531477/150‑1 (MQ=60)
                                                                                                                          ATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTC             >  SRR13214403.764934/1‑150 (MQ=60)
                                                                                                                          ATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTC             >  SRR13214403.764725/1‑150 (MQ=60)
                                                                                                                          ATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTC             <  SRR13214403.4126299/150‑1 (MQ=60)
                                                                                                                          ATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTC             <  SRR13214403.547845/150‑1 (MQ=60)
                                                                                                                          ATGGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTC             <  SRR13214403.4125939/150‑1 (MQ=60)
                                                                                                                            GGATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAG           >  SRR13214403.2026658/1‑150 (MQ=60)
                                                                                                                             GATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGT          <  SRR13214403.2007484/150‑1 (MQ=60)
                                                                                                                             GATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGT          <  SRR13214403.1987210/150‑1 (MQ=60)
                                                                                                                              ATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTT         <  SRR13214403.2449082/150‑1 (MQ=60)
                                                                                                                              ATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTT         <  SRR13214403.2449850/150‑1 (MQ=60)
                                                                                                                              ATTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTT         <  SRR13214403.1541616/150‑1 (MQ=60)
                                                                                                                              ATTCAAATCACATGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTT         <  SRR13214403.1541727/150‑1 (MQ=60)
                                                                                                                               TTCAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTG        <  SRR13214403.917231/150‑1 (MQ=60)
                                                                                                                                 CAAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCA      >  SRR13214403.2531672/1‑150 (MQ=60)
                                                                                                                                  AAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAA     >  SRR13214403.3574906/1‑150 (MQ=60)
                                                                                                                                  AAATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTAAGTTTTTTTATATTCAGTTGCAA     >  SRR13214403.4451219/1‑150 (MQ=60)
                                                                                                                                    ATCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAATT   <  SRR13214403.3224156/150‑1 (MQ=60)
                                                                                                                                     TCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAATTA  <  SRR13214403.2058032/150‑1 (MQ=60)
                                                                                                                                     TCACTTGACGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAACTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAATTA  <  SRR13214403.364930/150‑1 (MQ=60)
                                                                                                                                     |                                                                                                                                                     
AGGAAATAA‑‑ATATTATGGCAAATGGAACAGTAAAATGGTTTAACGCAGATAAAGGATTTGGCTTTATCACTTCAGAAGAAGGCAAAGATTTGTTCGCTCACTTCTCAGCAATCCAATCTGATGGATTCAAAACACTTGATGAAGGTCAAAAAGTTGAATTTGATGTTGAAGAAGGTCAACGTGGACCTCAAGCAGTTAATATCACAAAAGCATAATTGTATCTGAAAATCGTGCAAAAGCTGGAGTCATGACTTCAGTTTTTTTATATTCAGTTGCAATGA  >  CP043523/1848278‑1848558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑