Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F25 I0 R1 244 64.6 2208384 98.0% 2164216 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 9,058 C→A 65.1% A274E (GCG→GAG)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092739,0580CA65.1% 185.9 / 197.9 163A274E (GCG→GAG) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base C (32/25);  new base A (44/62);  total (76/87)
Fisher's exact test for biased strand distribution p-value = 9.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.58e-02

Only 100 of 173 total aligned reads displayed.

CGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGAC  >  CP009273/8909‑9207
                                                                                                                                                     |                                                                                                                                                     
cgcAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGgc                                                                                                                                                       <  1:120129/150‑1 (MQ=255)
   aaGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGagcg                                                                                                                                                    >  2:621022/1‑150 (MQ=255)
    aGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGcgcg                                                                                                                                                    >  2:414501/1‑149 (MQ=255)
     gCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTa                                                                                                                                                  <  2:640212/150‑1 (MQ=255)
       ttCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGCAGGTGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAt                                                                                                                                                 >  2:42070/1‑149 (MQ=255)
       ttCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAt                                                                                                                                                 >  2:92136/1‑149 (MQ=255)
       ttCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAt                                                                                                                                                 >  2:586887/1‑149 (MQ=255)
        tCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAtta                                                                                                                                               >  1:177721/1‑148 (MQ=255)
         ccGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAtta                                                                                                                                               <  2:879361/149‑3 (MQ=255)
         ccGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAtt                                                                                                                                                <  1:660515/148‑2 (MQ=255)
         ccGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAtt                                                                                                                                                <  2:56540/148‑2 (MQ=255)
         ccGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCa                                                                                                                                               <  1:940576/149‑1 (MQ=255)
           gTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTAccg                                                                                                                                            <  2:322845/150‑3 (MQ=255)
            tAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACt                                                                                                                                             >  1:201735/1‑148 (MQ=255)
              aCATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAg                                                                                                                                          <  1:831130/149‑1 (MQ=255)
              aCATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGt                                                                                                                                         <  2:205845/150‑1 (MQ=255)
                gttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATct                                                                                                                                       <  2:1025013/147‑2 (MQ=255)
                gttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATct                                                                                                                                       <  2:788748/147‑2 (MQ=255)
                 ttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATctg                                                                                                                                      <  1:6628/148‑3 (MQ=255)
                   ggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAg                                                                                                                                    >  1:435991/1‑150 (MQ=255)
                   ggCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGa                                                                                                                                     <  1:299565/149‑1 (MQ=255)
                    gCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAg                                                                                                                                    <  2:250535/149‑1 (MQ=255)
                    gCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGt                                                                                                                                   <  2:714249/150‑1 (MQ=255)
                      gAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTc                                                                                                                                 >  1:963083/1‑150 (MQ=255)
                      gAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTc                                                                                                                                 >  1:674635/1‑150 (MQ=255)
                          ttCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCtgt                                                                                                                             >  1:999197/1‑150 (MQ=255)
                          ttCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCtgt                                                                                                                             >  2:938185/1‑150 (MQ=255)
                                  ctggctggCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGgcagca                                                                                                                      >  1:1079088/1‑149 (MQ=255)
                                     gctggCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGgcagcata                                                                                                                    <  1:983101/145‑3 (MQ=255)
                                       aGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAAc                                                                                                                  >  1:809919/1‑148 (MQ=255)
                                       aGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAAc                                                                                                                  >  1:561897/1‑148 (MQ=255)
                                          cTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGa                                                                                                             >  2:753028/1‑150 (MQ=255)
                                            gCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATc                                                                                                           >  1:602020/1‑150 (MQ=255)
                                              gACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATcc                                                                                                          >  2:774188/1‑149 (MQ=255)
                                                     tGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAg                                                                                                  >  1:420188/1‑150 (MQ=255)
                                                      gACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGcgg                                                                                                  >  1:333015/1‑147 (MQ=255)
                                                      gACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAg                                                                                                  >  1:561107/1‑149 (MQ=255)
                                                       aCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAg                                                                                                  <  1:172128/148‑1 (MQ=255)
                                                          aTCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGa                                                                                              >  2:867244/1‑149 (MQ=255)
                                                           tCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGaca                                                                                            <  1:762889/150‑3 (MQ=255)
                                                               cccGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACt                                                                                        <  2:281607/149‑1 (MQ=255)
                                                               aCCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACt                                                                                        >  1:449882/1‑150 (MQ=255)
                                                                  ggCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGc                                                                                     >  1:106115/1‑150 (MQ=255)
                                                                  ggCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGc                                                                                     >  1:372793/1‑150 (MQ=255)
                                                                  ggCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGc                                                                                     >  1:710250/1‑150 (MQ=255)
                                                                   gcgctgctgAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCg                                                                                    <  1:52762/147‑1 (MQ=255)
                                                                       tgctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAg                                                                                >  1:509273/1‑150 (MQ=255)
                                                                         ctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAg                                                                                <  1:152224/148‑1 (MQ=255)
                                                                          tgAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTa                                                                             >  1:38626/1‑150 (MQ=255)
                                                                            aaaGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATc                                                                           <  2:652504/150‑1 (MQ=255)
                                                                              agagCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCg                                                                         <  2:476809/150‑1 (MQ=255)
                                                                                    ggCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGtt                                                                   >  1:25737/1‑150 (MQ=255)
                                                                                     tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGt                                                                    <  1:462632/142‑1 (MQ=255)
                                                                                     tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACCGGCGGAAGGTATCCGTAAGtt                                                                   <  2:299776/143‑1 (MQ=255)
                                                                                        gaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGc                                                                >  1:714998/4‑149 (MQ=255)
                                                                                           agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                            >  1:245764/1‑149 (MQ=255)
                                                                                           agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                            <  2:272194/150‑2 (MQ=255)
                                                                                           agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                            <  2:494413/150‑2 (MQ=255)
                                                                                                    gCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGa                                                    >  1:172855/1‑149 (MQ=255)
                                                                                                         cGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACt                                              >  2:825004/1‑150 (MQ=255)
                                                                                                         cGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACt                                              >  2:553325/1‑150 (MQ=255)
                                                                                                          gAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTg                                             >  2:594444/1‑150 (MQ=255)
                                                                                                            aCGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTgg                                            >  1:361969/1‑149 (MQ=255)
                                                                                                            aCGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGa                                           <  1:361511/150‑1 (MQ=255)
                                                                                                             cGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGa                                           >  1:711518/1‑149 (MQ=255)
                                                                                                                  aCTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGCATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAAt                                     >  1:543278/1‑150 (MQ=255)
                                                                                                                   cTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATg                                    <  1:756897/150‑1 (MQ=255)
                                                                                                                     ggtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGAt                                  >  2:20037/8‑150 (MQ=255)
                                                                                                                      tCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATc                                 <  2:1026417/150‑1 (MQ=255)
                                                                                                                      gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGAt                                  <  2:125647/143‑1 (MQ=255)
                                                                                                                       tatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCg                                <  1:775855/145‑1 (MQ=255)
                                                                                                                        atatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCga                               >  2:291235/5‑149 (MQ=255)
                                                                                                                         tatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCga                               <  2:833806/146‑2 (MQ=255)
                                                                                                                         tatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCga                               <  1:888565/146‑2 (MQ=255)
                                                                                                                         tatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCga                               <  1:398176/146‑2 (MQ=255)
                                                                                                                         tatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGC‑GAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                             <  2:333015/147‑1 (MQ=255)
                                                                                                                          acacCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGc                              >  1:519305/1‑149 (MQ=255)
                                                                                                                           tacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                             >  1:101867/2‑149 (MQ=255)
                                                                                                                           cacCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGa                            <  1:414501/150‑1 (MQ=255)
                                                                                                                            acCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATcgacgaa                           >  1:761607/1‑149 (MQ=255)
                                                                                                                            acCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGAt                           >  2:1068327/1‑150 (MQ=255)
                                                                                                                             ccGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATcgacgaa                           <  2:573323/149‑2 (MQ=255)
                                                                                                                                 tgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgct                      >  2:168362/2‑150 (MQ=255)
                                                                                                                                   aaGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                    <  1:377873/150‑1 (MQ=255)
                                                                                                                                     gTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTaa                  >  1:26951/1‑150 (MQ=255)
                                                                                                                                      tGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAAt                 >  1:608719/1‑150 (MQ=255)
                                                                                                                                       gAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaa                  >  1:941018/1‑148 (MQ=255)
                                                                                                                                       gAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaa                  >  1:830696/1‑148 (MQ=255)
                                                                                                                                        aaaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagag               <  1:1007658/150‑4 (MQ=255)
                                                                                                                                         aaGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCAt              <  2:561107/150‑1 (MQ=255)
                                                                                                                                         aaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagag               >  1:248797/1‑146 (MQ=255)
                                                                                                                                         aaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagag               >  1:287084/1‑146 (MQ=255)
                                                                                                                                          aGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaa             <  2:231946/150‑6 (MQ=255)
                                                                                                                                           gcgcGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTc            >  2:119584/1‑150 (MQ=255)
                                                                                                                                           gcgcGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaat            <  2:670215/150‑7 (MQ=255)
                                                                                                                                            cgcgTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaata           <  2:696908/150‑8 (MQ=255)
                                                                                                                                            cgcgTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaata           <  2:145439/150‑8 (MQ=255)
                                                                                                                                            cgcgTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaa             >  1:1035324/1‑143 (MQ=255)
                                                                                                                                                     cgcgTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGAc  <  2:449882/150‑1 (MQ=255)
                                                                                                                                                     cgcgTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGAc  <  2:172855/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGAC  >  CP009273/8909‑9207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACC  >  CP009273/8908‑9208
                                                                                                                                                      |                                                                                                                                                      
GCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGC                                                                                                                                                        <  SRR12117904.120699/151‑1 (MQ=60)
 CGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCG                                                                                                                                                       <  SRR12117904.375268/151‑1 (MQ=60)
    AAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGT                                                                                                                                                    >  SRR12117904.1031572/1‑151 (MQ=60)
     AGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGT                                                                                                                                                    >  SRR12117904.717708/1‑150 (MQ=60)
      GCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAT                                                                                                                                                  >  SRR12117904.236836/1‑151 (MQ=31)
      GCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAT                                                                                                                                                  <  SRR12117904.258061/151‑1 (MQ=31)
         TCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCA                                                                                                                                                <  SRR12117904.945234/150‑1 (MQ=60)
          CCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACT                                                                                                                                              <  SRR12117904.1105848/151‑1 (MQ=60)
             TAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTG                                                                                                                                             >  SRR12117904.202703/1‑149 (MQ=60)
              AACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAG                                                                                                                                           <  SRR12117904.835262/150‑1 (MQ=60)
                   CGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGA                                                                                                                                      <  SRR12117904.300980/150‑1 (MQ=60)
                                        AGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACC                                                                                                                  >  SRR12117904.813942/1‑149 (MQ=60)
                                        AGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACC                                                                                                                  >  SRR12117904.564598/1‑149 (MQ=60)
                                         GGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAG                                                                                                                <  SRR12117904.727583/150‑1 (MQ=13)
                                             gtgaccgcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCC                                                                                                           >  SRR12117904.601166/81‑151 (MQ=33)
                                             gtgaccgcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCC                                                                                                           <  SRR12117904.689574/71‑1 (MQ=33)
                                             GCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCC                                                                                                           >  SRR12117904.604936/1‑151 (MQ=60)
                                              tgaccgcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCG                                                                                                          >  SRR12117904.496785/80‑151 (MQ=33)
                                              tgaccgcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCG                                                                                                          >  SRR12117904.1059381/80‑151 (MQ=33)
                                                 ccgcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATG                                                                                                       >  SRR12117904.1011038/77‑151 (MQ=32)
                                                  cgcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGG                                                                                                      >  SRR12117904.155878/76‑151 (MQ=31)
                                                   gcctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGC                                                                                                     <  SRR12117904.847241/77‑1 (MQ=33)
                                                    cctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCG                                                                                                    >  SRR12117904.837950/74‑151 (MQ=33)
                                                    cctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCG                                                                                                    <  SRR12117904.203097/78‑1 (MQ=33)
                                                     ctgaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGG                                                                                                   >  SRR12117904.95594/73‑151 (MQ=33)
                                                      TGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGT                                                                                                  >  SRR12117904.422178/1‑151 (MQ=60)
                                                       gaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGT                                                                                                  <  SRR12117904.713200/80‑1 (MQ=32)
                                                       gaccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGT                                                                                                  >  SRR12117904.334609/71‑150 (MQ=32)
                                                       GACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGT                                                                                                  >  SRR12117904.563803/1‑150 (MQ=60)
                                                       GACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAG                                                                                                   <  SRR12117904.172927/149‑1 (MQ=60)
                                                        accatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAG                                                                                                <  SRR12117904.843425/82‑1 (MQ=32)
                                                         ccatcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGT                                                                                                  >  SRR12117904.162173/69‑148 (MQ=32)
                                                           atcgccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACA                                                                                             <  SRR12117904.766663/85‑1 (MQ=33)
                                                              gccccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAAC                                                                                          <  SRR12117904.4147/88‑1 (MQ=32)
                                                                cccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTG                                                                                        >  SRR12117904.567356/62‑151 (MQ=35)
                                                                ACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTG                                                                                        >  SRR12117904.452028/1‑151 (MQ=60)
                                                                 ccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGG                                                                                       >  SRR12117904.126238/61‑151 (MQ=37)
                                                                 ccggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGG                                                                                       >  SRR12117904.354641/61‑151 (MQ=37)
                                                                  cggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGC                                                                                      <  SRR12117904.458595/92‑1 (MQ=38)
                                                                   ggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCG                                                                                     <  SRR12117904.53020/93‑1 (MQ=40)
                                                                   ggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCG                                                                                     >  SRR12117904.374589/59‑151 (MQ=40)
                                                                   ggcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCG                                                                                     >  SRR12117904.106605/59‑151 (MQ=40)
                                                                   GGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCG                                                                                     >  SRR12117904.713684/1‑151 (MQ=60)
                                                                    gcgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGG                                                                                    <  SRR12117904.84887/94‑1 (MQ=42)
                                                                     cgctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGG                                                                                    >  SRR12117904.981630/57‑150 (MQ=42)
                                                                      gctgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGA                                                                                   >  SRR12117904.19204/56‑150 (MQ=44)
                                                                        tgctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGG                                                                                >  SRR12117904.736478/54‑151 (MQ=49)
                                                                        TGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGG                                                                                >  SRR12117904.511713/1‑151 (MQ=60)
                                                                         GCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAG                                                                                 <  SRR12117904.152920/149‑1 (MQ=60)
                                                                          ctgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGT                                                                               >  SRR12117904.80986/52‑150 (MQ=50)
                                                                           tgaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTAT                                                                             >  SRR12117904.38810/51‑151 (MQ=54)
                                                                            gaaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTA                                                                              <  SRR12117904.983198/100‑1 (MQ=52)
                                                                             aaagagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCC                                                                           >  SRR12117904.165119/49‑151 (MQ=57)
                                                                                gagctgtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGT                                                                         >  SRR12117904.273499/46‑150 (MQ=60)
                                                                                     gtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTT                                                                   <  SRR12117904.384361/111‑1 (MQ=60)
                                                                                     gtccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGT                                                                     <  SRR12117904.464835/109‑1 (MQ=60)
                                                                                     GGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTT                                                                   >  SRR12117904.41481/1‑151 (MQ=60)
                                                                                     GGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTT                                                                   >  SRR12117904.25860/1‑151 (MQ=60)
                                                                                       ccgaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGC                                                                 <  SRR12117904.367386/113‑1 (MQ=60)
                                                                                         gaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGA                                                               <  SRR12117904.1043366/115‑1 (MQ=60)
                                                                                         gaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCG                                                                >  SRR12117904.718459/37‑150 (MQ=60)
                                                                                         gaaagcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCG                                                                >  SRR12117904.827519/37‑150 (MQ=60)
                                                                                            agcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCgatcg                                                            <  SRR12117904.194946/118‑6 (MQ=60)
                                                                                            agcgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCgatcg                                                            >  SRR12117904.246951/34‑146 (MQ=60)
                                                                                            AGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTG                                                            <  SRR12117904.349640/151‑1 (MQ=60)
                                                                                              cgaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGAC                                                          >  SRR12117904.545727/32‑151 (MQ=60)
                                                                                               gaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACC                                                         >  SRR12117904.737893/31‑151 (MQ=60)
                                                                                               gaaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGATTGCGATCGACC                                                         >  SRR12117904.413732/31‑151 (MQ=59)
                                                                                                aaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGGCAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCA                                                        <  SRR12117904.84150/122‑1 (MQ=59)
                                                                                                aaggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCA                                                        <  SRR12117904.245577/122‑1 (MQ=60)
                                                                                                 aggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAG                                                       <  SRR12117904.373127/123‑1 (MQ=60)
                                                                                                  ggcgccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGG                                                      >  SRR12117904.131409/28‑151 (MQ=60)
                                                                                                     gccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAA                                                   <  SRR12117904.79255/127‑1 (MQ=60)
                                                                                                     gccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAA                                                    >  SRR12117904.926563/25‑150 (MQ=60)
                                                                                                     gccgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAA                                                    >  SRR12117904.36114/25‑150 (MQ=60)
                                                                                                     GCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAA                                                    >  SRR12117904.173658/1‑150 (MQ=60)
                                                                                                       cgttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAA                                                  >  SRR12117904.301478/23‑150 (MQ=60)
                                                                                                        gttgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAAC                                                >  SRR12117904.786705/22‑151 (MQ=60)
                                                                                                          tgagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTG                                              >  SRR12117904.693323/20‑151 (MQ=60)
                                                                                                          CGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTG                                              <  SRR12117904.560129/151‑1 (MQ=60)
                                                                                                           gagcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGG                                             >  SRR12117904.59838/19‑151 (MQ=60)
                                                                                                            AACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGA                                            <  SRR12117904.363241/151‑1 (MQ=60)
                                                                                                             gcgtaagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAA                                           <  SRR12117904.516469/135‑1 (MQ=60)
                                                                                                             ACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGA                                            >  SRR12117904.363700/1‑150 (MQ=60)
                                                                                                              CGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAA                                           >  SRR12117904.714961/1‑150 (MQ=60)
                                                                                                                 aagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAA                                       <  SRR12117904.1049065/139‑1 (MQ=60)
                                                                                                                 aagctggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAA                                        <  SRR12117904.60802/138‑1 (MQ=60)
                                                                                                                   ACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATG                                     <  SRR12117904.760641/151‑1 (MQ=60)
                                                                                                                   ACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGCATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATG                                     >  SRR12117904.545899/1‑151 (MQ=60)
                                                                                                                      ggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAATTTTGCGATCGACCAGGAAAAACTGGAAAcactgctc                                  >  SRR12117904.390689/8‑143 (MQ=60)
                                                                                                                      ggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATC                                  <  SRR12117904.175138/144‑1 (MQ=60)
                                                                                                                      ggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATC                                  <  SRR12117904.260898/144‑1 (MQ=60)
                                                                                                                      ggtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGAT                                   <  SRR12117904.986734/143‑1 (MQ=60)
                                                                                                                      GTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATC                                  <  SRR12117904.778025/151‑1 (MQ=60)
                                                                                                                       gtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCG                                 <  SRR12117904.456206/145‑1 (MQ=60)
                                                                                                                       gtatatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCG                                 <  SRR12117904.779701/145‑1 (MQ=60)
                                                                                                                         atatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGAC                               <  SRR12117904.292605/147‑1 (MQ=60)
                                                                                                                         atatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGA                                <  SRR12117904.329289/146‑1 (MQ=60)
                                                                                                                         atatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGA                                <  SRR12117904.892970/146‑1 (MQ=60)
                                                                                                                         atatACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGA                                <  SRR12117904.400082/146‑1 (MQ=60)
                                                                                                                          TACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCG                                 <  SRR12117904.498589/148‑1 (MQ=60)
                                                                                                                           ATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGA                             <  SRR12117904.666524/151‑1 (MQ=60)
                                                                                                                           ACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGA                             <  SRR12117904.416470/151‑1 (MQ=60)
                                                                                                                           ACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCG                              >  SRR12117904.521816/1‑150 (MQ=60)
                                                                                                                           ACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCG                              <  SRR12117904.942828/150‑1 (MQ=60)
                                                                                                                            TACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGA                             >  SRR12117904.102342/1‑150 (MQ=60)
                                                                                                                             ACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAAC                           <  SRR12117904.713400/151‑1 (MQ=60)
                                                                                                                             ACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAAC                           >  SRR12117904.765369/1‑151 (MQ=60)
                                                                                                                             ACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAAC                           <  SRR12117904.609755/151‑1 (MQ=60)
                                                                                                                                   GAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGT                     <  SRR12117904.379689/151‑1 (MQ=60)
                                                                                                                                   GAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTG                      <  SRR12117904.417712/150‑1 (MQ=60)
                                                                                                                                      GTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAAT                  >  SRR12117904.27081/1‑151 (MQ=60)
                                                                                                                                       TGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATC                 >  SRR12117904.611660/1‑151 (MQ=60)
                                                                                                                                       TGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTA                    >  SRR12117904.764120/1‑148 (MQ=60)
                                                                                                                                        GAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgag                <  SRR12117904.1012706/151‑4 (MQ=60)
                                                                                                                                        GAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAG                  >  SRR12117904.834824/1‑149 (MQ=60)
                                                                                                                                        GAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAG                  >  SRR12117904.945683/1‑149 (MQ=60)
                                                                                                                                         AAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAG                  >  SRR12117904.997500/1‑148 (MQ=60)
                                                                                                                                          AAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgaga               >  SRR12117904.288434/1‑146 (MQ=60)
                                                                                                                                          AAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgaga               >  SRR12117904.249995/1‑146 (MQ=60)
                                                                                                                                           AGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaat             >  SRR12117904.253930/1‑145 (MQ=60)
                                                                                                                                            GCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaata            <  SRR12117904.447232/151‑8 (MQ=60)
                                                                                                                                             CGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTT           >  SRR12117904.527936/1‑151 (MQ=60)
                                                                                                                                             CGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaat             >  SRR12117904.1040518/1‑143 (MQ=60)
                                                                                                                                               CGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaatagaa         <  SRR12117904.1056787/151‑11 (MQ=60)
                                                                                                                                               CGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaataga          >  SRR12117904.874719/1‑141 (MQ=60)
                                                                                                                                                GTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaatagaaa        <  SRR12117904.507981/151‑12 (MQ=60)
                                                                                                                                                GTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaatagaaa        <  SRR12117904.289912/151‑12 (MQ=60)
                                                                                                                                                  CCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaatagaaagg      <  SRR12117904.647553/151‑14 (MQ=60)
                                                                                                                                                  CCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTAAgagaatagaaagg      <  SRR12117904.344180/151‑14 (MQ=60)
                                                                                                                                                      CGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACC  <  SRR12117904.913026/151‑1 (MQ=60)
                                                                                                                                                      |                                                                                                                                                      
GCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACC  >  CP009273/8908‑9208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑