breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 8,261 T→G 5.7% L8L (CTT→CTG talB → transaldolase B
RA 8,276 C→T 12.6% T13T (ACC→ACT talB → transaldolase B
RA 8,279 A→C 12.0% V14V (GTA→GTC talB → transaldolase B
RA 8,282 G→C 12.3% V15V (GTG→GTC talB → transaldolase B
RA 8,285 C→T 12.5% A16A (GCC→GCT talB → transaldolase B
RA 8,291 T→C 11.7% T18T (ACT→ACC talB → transaldolase B
RA 8,294 G→A 13.0% G19G (GGG→GGA talB → transaldolase B
RA 8,297 C→T 11.9% D20D (GAC→GAT talB → transaldolase B
RA 8,300 C→T 12.0% I21I (ATC→ATT talB → transaldolase B
RA 8,321 A→G 15.0% Q28Q (CAA→CAG talB → transaldolase B
RA 8,324 G→T 15.1% P29P (CCG→CCT talB → transaldolase B
RA 8,336 A→C 21.3% T33T (ACA→ACC talB → transaldolase B
RA 8,339 C→T 22.6% T34T (ACC→ACT talB → transaldolase B
RA 8,348 T→C 26.0% S37S (TCT→TCC talB → transaldolase B
RA 8,351 C→G 26.0% L38L (CTC→CTG talB → transaldolase B
RA 8,357 T→C 26.0% L40L (CTT→CTC talB → transaldolase B
RA 8,358 A→G 26.0% N41D (AAC→GAC) ‡ talB → transaldolase B
RA 8,359 A→G 26.1% N41S (AAC→AGC) ‡ talB → transaldolase B
RA 8,363 A→G 26.6% A42A (GCA→GCG talB → transaldolase B
RA 8,366 G→T 26.6% A43A (GCG→GCT talB → transaldolase B
RA 8,372 T→C 30.2% I45I (ATT→ATC talB → transaldolase B
RA 8,375 G→T 29.7% P46P (CCG→CCT talB → transaldolase B
RA 8,378 A→G 30.5% E47E (GAA→GAG talB → transaldolase B
RA 8,388 T→C 33.4% L51L (TTG→CTG)  talB → transaldolase B
RA 8,396 T→C 34.2% D53D (GAT→GAC talB → transaldolase B
RA 8,399 T→C 35.9% D54D (GAT→GAC talB → transaldolase B
RA 8,414 G→T 30.8% A59A (GCG→GCT talB → transaldolase B
RA 8,415 A→C 31.4% K60Q (AAA→CAA) ‡ talB → transaldolase B
RA 8,416 A→G 32.2% K60R (AAA→AGA) ‡ talB → transaldolase B
RA 8,417 A→T 31.7% K60N (AAA→AAT) ‡ talB → transaldolase B
RA 8,419 A→G 32.2% Q61R (CAG→CGG) ‡ talB → transaldolase B
RA 8,420 G→C 32.2% Q61H (CAG→CAC) ‡ talB → transaldolase B
RA 8,428 A→G 35.8% N64S (AAC→AGC)  talB → transaldolase B
RA 8,448 G→C 41.4% V71L (GTG→CTG) ‡ talB → transaldolase B
RA 8,450 G→C 41.0% V71V (GTG→GTC) ‡ talB → transaldolase B
RA 8,453 C→T 41.3% D72D (GAC→GAT talB → transaldolase B
RA 8,456 G→T 43.5% A73A (GCG→GCT talB → transaldolase B
RA 8,457 A→T 43.0% T74S (ACC→TCC)  talB → transaldolase B
RA 8,462 C→T 43.0% D75D (GAC→GAT talB → transaldolase B
RA 8,465 A→G 43.6% K76K (AAA→AAG talB → transaldolase B
RA 8,471 A→G 44.8% A78A (GCA→GCG talB → transaldolase B
RA 8,474 A→G 44.0% V79V (GTA→GTG talB → transaldolase B
RA 8,477 T→C 42.7% N80N (AAT→AAC talB → transaldolase B
RA 8,480 T→C 41.8% I81I (ATT→ATC talB → transaldolase B
RA 8,486 G→C 43.2% L83L (CTG→CTC talB → transaldolase B
RA 8,492 C→T 44.3% I85I (ATC→ATT talB → transaldolase B
RA 8,502 G→A 48.2% V89I (GTT→ATT) ‡ talB → transaldolase B
RA 8,504 T→C 47.8% V89V (GTT→GTC) ‡ talB → transaldolase B
RA 8,519 A→C 47.2% S94S (TCA→TCC talB → transaldolase B
RA 8,522 T→C 47.2% T95T (ACT→ACC talB → transaldolase B
RA 8,528 T→G 51.7% V97V (GTT→GTG talB → transaldolase B
RA 8,531 T→C 50.0% D98D (GAT→GAC talB → transaldolase B
RA 8,534 G→A 49.7% A99A (GCG→GCA talB → transaldolase B
RA 8,540 T→C 51.0% L101L (CTT→CTC talB → transaldolase B
RA 8,561 A→T 60.2% S108S (TCA→TCT talB → transaldolase B
RA 8,564 T→C 60.2% I109I (ATT→ATC talB → transaldolase B
RA 8,573 A→C 63.0% A112A (GCA→GCC talB → transaldolase B
RA 8,576 A→G 62.4% K113K (AAA→AAG talB → transaldolase B
RA 8,580 C→A 62.3% L115M (CTG→ATG) ‡ talB → transaldolase B
RA 8,582 G→C 63.0% L115L (CTG→CTC) ‡ talB → transaldolase B
RA 8,588 A→G 65.2% K117K (AAA→AAG talB → transaldolase B
RA 8,591 C→G 63.8% L118L (CTC→CTG talB → transaldolase B
RA 8,600 T→C 67.2% D121D (GAT→GAC talB → transaldolase B
RA 8,603 T→G 66.9% A122A (GCT→GCG talB → transaldolase B
RA 8,606 T→C 66.4% G123G (GGT→GGC talB → transaldolase B
RA 8,609 T→C 66.5% I124I (ATT→ATC talB → transaldolase B
RA 8,621 T→C 67.6% R128R (CGT→CGC talB → transaldolase B
RA 8,639 T→C 68.7% A134A (GCT→GCC talB → transaldolase B
RA 8,642 T→C 68.6% S135S (TCT→TCC talB → transaldolase B
RA 8,654 T→C 70.9% G139G (GGT→GGC talB → transaldolase B
RA 8,657 C→T 71.1% I140I (ATC→ATT talB → transaldolase B
RA 8,660 T→C 71.9% R141R (CGT→CGC talB → transaldolase B
RA 8,663 T→C 71.5% A142A (GCT→GCC talB → transaldolase B
RA 8,666 A→T 70.8% A143A (GCA→GCT talB → transaldolase B
RA 8,690 C→T 73.3% I151I (ATC→ATT talB → transaldolase B
RA 8,705 C→G 72.6% T156T (ACC→ACG talB → transaldolase B
RA 8,732 T→C 72.6% R165R (CGT→CGC talB → transaldolase B
RA 8,738 T→C 72.7% C167C (TGT→TGC talB → transaldolase B
RA 8,741 G→C 72.9% A168A (GCG→GCC talB → transaldolase B
RA 8,753 G→C 75.3% V172V (GTG→GTC talB → transaldolase B
RA 8,762 C→T 76.2% I175I (ATC→ATT talB → transaldolase B
RA 8,771 T→C 77.0% F178F (TTT→TTC talB → transaldolase B
RA 8,783 T→C 79.0% I182I (ATT→ATC talB → transaldolase B
RA 8,786 T→C 78.4% L183L (CTT→CTC talB → transaldolase B
RA 8,801 G→C 79.4% A188A (GCG→GCC talB → transaldolase B
RA 8,804 T→C 79.2% N189N (AAT→AAC talB → transaldolase B
RA 8,810 T→C 80.0% D191D (GAT→GAC talB → transaldolase B
RA 8,825 T→A 79.6% A196A (GCT→GCA talB → transaldolase B
RA 8,852 T→C 78.5% S205S (TCT→TCC talB → transaldolase B
RA 8,855 A→C 77.7% V206V (GTA→GTC talB → transaldolase B
RA 8,856 T→A 77.7% S207T (TCT→ACT) ‡ talB → transaldolase B
RA 8,857 C→G 76.7% S207C (TCT→TGT) ‡ talB → transaldolase B
RA 8,858 T→C 76.8% S207S (TCT→TCC) ‡ talB → transaldolase B
RA 8,868 C→G 78.2% Q211E (CAG→GAG)  talB → transaldolase B
RA 8,873 C→T 78.4% Y212Y (TAC→TAT talB → transaldolase B
RA 8,879 A→G 76.6% K214K (AAA→AAG talB → transaldolase B
RA 8,880 G→C 76.6% E215Q (GAG→CAG)  talB → transaldolase B
RA 8,888 T→C 75.3% G217G (GGT→GGC talB → transaldolase B
RA 8,900 G→C 72.5% V221V (GTG→GTC talB → transaldolase B
RA 8,903 T→G 72.6% V222V (GTT→GTG talB → transaldolase B
RA 8,925 A→G 70.0% I230V (ATC→GTC) ‡ talB → transaldolase B
RA 8,927 C→T 70.0% I230I (ATC→ATT) ‡ talB → transaldolase B
RA 8,948 A→T 66.8% A237A (GCA→GCT talB → transaldolase B
RA 8,954 C→T 67.8% C239C (TGC→TGT talB → transaldolase B
RA 8,960 T→C 67.0% R241R (CGT→CGC talB → transaldolase B
RA 8,972 A→C 64.9% A245A (GCA→GCC talB → transaldolase B
RA 8,978 A→G 64.0% A247A (GCA→GCG talB → transaldolase B
RA 8,994 G→T 64.4% A253S (GCG→TCG) ‡ talB → transaldolase B
RA 8,996 G→C 63.9% A253A (GCG→GCC) ‡ talB → transaldolase B
RA 8,999 G→A 64.0% E254E (GAG→GAA talB → transaldolase B
RA 9,008 G→C 64.1% G257G (GGG→GGC talB → transaldolase B
RA 9,011 T→C 63.8% A258A (GCT→GCC talB → transaldolase B
RA 9,012 A→G 63.9% I259V (ATC→GTC) ‡ talB → transaldolase B
RA 9,014 C→T 63.9% I259I (ATC→ATT) ‡ talB → transaldolase B
RA 9,017 A→G 62.3% E260E (GAA→GAG talB → transaldolase B
RA 9,023 A→G 62.6% K262K (AAA→AAG talB → transaldolase B
RA 9,027 T→G 58.6% S264A (TCT→GCT) ‡ talB → transaldolase B
RA 9,028 C→T 57.8% S264F (TCT→TTT) ‡ talB → transaldolase B
RA 9,029 T→A 58.6% S264S (TCT→TCA) ‡ talB → transaldolase B
RA 9,032 C→T 60.3% Y265Y (TAC→TAT talB → transaldolase B
RA 9,038 C→T 64.3% G267G (GGC→GGT talB → transaldolase B
RA 9,058 C→A 65.1% A274E (GCG→GAG)  talB → transaldolase B
RA 9,065 C→T 62.1% I276I (ATC→ATT talB → transaldolase B
RA 9,068 T→C 62.6% T277T (ACT→ACC talB → transaldolase B
RA 9,071 G→A 62.6% E278E (GAG→GAA talB → transaldolase B
RA 9,074 C→T 61.8% S279S (TCC→TCT talB → transaldolase B
RA 9,092 C→T 57.1% H285H (CAC→CAT talB → transaldolase B
RA 9,104 A→G 56.2% P289P (CCA→CCG talB → transaldolase B
RA 9,110 A→G 55.1% A291A (GCA→GCG talB → transaldolase B
RA 9,116 T→C 54.6% D293D (GAT→GAC talB → transaldolase B
RA 9,146 T→G 46.4% A303A (GCT→GCG talB → transaldolase B
RA 9,149 T→C 44.1% I304I (ATT→ATC talB → transaldolase B
RA 9,178 G→A 32.2% G314D (GGC→GAC)  talB → transaldolase B
RA 9,182 T→A 31.0% D315E (GAT→GAA talB → transaldolase B
RA 360,147 G→C 24.6% intergenic (‑81/+514) lacZ ← / ← lacI pseudogene, truncated/DNA‑binding transcriptional repressor
JC 374,639 +GCCC 100% coding (389/1110 nt) frmA ← alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase
RA 374,648 Δ1 bp 25.5% coding (380/1110 nt) frmA ← alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase
RA 455,197 C→T 12.6% S285L (TCG→TTG)  lon → DNA‑binding ATP‑dependent protease La
RA 684,503:1 +A 14.3% intergenic (‑34/+296) insH1 ← / ← lnt IS5 transposase and trans‑activator/apolipoprotein N‑acyltransferase
JC 750,921 +GCTATCT 12.0% coding (289/390 nt) sdhC → succinate dehydrogenase, membrane subunit, binds cytochrome b556
JC JC 982,570 IS5 (–) +4 bp 24.4% intergenic (‑132/+468) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,191,676 C→T 32.4% H366H (CAC→CAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,688 C→T 30.3% T370T (ACC→ACT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,701 T→C 27.3% L375L (TTA→CTA) ‡ icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,703 A→G 25.0% L375L (TTA→TTG) ‡ icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,733 C→T 19.4% N385N (AAC→AAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,736 G→C 19.4% A386A (GCG→GCC icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,739 A→G 19.3% K387K (AAA→AAG icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,748 C→T 19.2% T390T (ACC→ACT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,230,349 T→C 39.7% intergenic (‑176/‑45) nhaB ← / → fadR sodium:proton antiporter/fatty acid metabolism regulon transcriptional regulator
RA 1,484,009 T→C 47.4% pseudogene (213/252 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,072 G→A 50.0% pseudogene (150/252 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,221:1 +T 100% pseudogene (1/252 nt)
pseudogene (750/750 nt)
gapC ←
gapC ←
pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,236 C→G 100% pseudogene (735/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,239 T→C 100% pseudogene (732/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,245 T→C 100% pseudogene (726/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,404 C→T 100% pseudogene (567/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,856 A→T 100% pseudogene (115/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,484,919 C→G 100% pseudogene (52/750 nt) gapC ← pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
RA 1,485,017 A→G 80.0% intergenic (‑47/‑142) gapC ← / → cybB pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)/cytochrome b561
RA 1,551,071 C→T 13.0% L64F (CTT→TTT)  osmC → lipoyl‑dependent Cys‑based peroxidase, hydroperoxide resistance; salt‑shock inducible membrane protein; peroxiredoxin
RA 1,551,119 A→C 18.8% T80P (ACC→CCC)  osmC → lipoyl‑dependent Cys‑based peroxidase, hydroperoxide resistance; salt‑shock inducible membrane protein; peroxiredoxin
RA 1,555,008 T→C 8.2% D60G (GAC→GGC)  ddpB ← D‑ala‑D‑ala transporter subunit
RA 1,837,194 T→A 22.9% N189K (AAT→AAA gdhA → glutamate dehydrogenase, NADP‑specific
RA 1,909,758 C→A 37.9% E12* (GAA→TAA)  proQ ← RNA chaperone, putative ProP translation regulator
RA 1,977,727 A→C 100% I275R (ATA→AGA)  araG ← fused L‑arabinose transporter subunits of ABC superfamily: ATP‑binding components
RA 2,033,608 T→C 16.1% L217P (CTC→CCC)  yedY → membrane‑anchored, periplasmic TMAO, DMSO reductase
MC JC 2,093,044 Δ2,186 bp 100% IS5‑mediated [ugd]wbbL [ugd], gnd, wbbL
RA 2,145,170 G→T 26.4% intergenic (+43/+22) yegJ → / ← yegK uncharacterized protein/ser/thr phosphatase‑related protein
JC JC 2,770,391 IS2 (+) +5 bp 47.0% coding (156‑160/201 nt) ypjJ → uncharacterized protein
RA 2,960,118:1 +C 66.0% coding (1676/2247 nt) ptsP ← fused PTS enzyme: PEP‑protein phosphotransferase (enzyme I)/GAF domain containing protein
RA 2,978,089 A→C 24.4% M288R (ATG→AGG)  yqeF ← short chain acyltransferase
JC JC 3,034,316 IS2 (+) +5 bp 41.1% coding (110‑114/267 nt) sdhE ← flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair
RA 3,074,446 A→G 6.7% D183D (GAT→GAC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,454 A→C 11.4% F181V (TTC→GTC)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,455 T→G 11.4% A180A (GCA→GCC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,467 A→G 15.2% G176G (GGT→GGC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,473 C→T 14.8% K174K (AAG→AAA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,478 G→T 14.5% L173M (CTG→ATG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,481 T→A 14.4% T172S (ACG→TCG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,482 A→G 14.6% G171G (GGT→GGC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,485 C→T 14.7% A170A (GCG→GCA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,490 G→A 15.0% L169L (CTG→TTG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,491 A→T 15.0% S168S (TCT→TCA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,498 A→G 15.6% V166A (GTT→GCT)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,503 G→A 17.4% H164H (CAC→CAT tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,506 G→C 18.0% S163S (TCC→TCG tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,521 C→T 24.6% M158I (ATG→ATA) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,523 T→A 24.6% M158L (ATG→TTG) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,538 T→G 28.9% M153L (ATG→CTG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,563 A→G 28.2% I144I (ATT→ATC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,566 G→T 28.4% D143E (GAC→GAA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,575 C→G 27.8% P140P (CCG→CCC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,578 A→G 27.4% R139R (CGT→CGC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,584 A→G 24.2% F137F (TTT→TTC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,587 C→T 24.0% Q136Q (CAG→CAA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,590 C→G 22.5% A135A (GCG→GCC) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,591 G→C 21.5% A135G (GCG→GGG) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,601 T→C 17.5% T132A (ACG→GCG)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,602 T→G 17.3% K131N (AAA→AAC) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,603 T→C 17.1% K131R (AAA→AGA) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,604 T→G 16.9% K131Q (AAA→CAA) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,608 T→G 15.4% A129A (GCA→GCC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,611 A→T 16.6% I128I (ATT→ATA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,617 C→G 17.1% M126I (ATG→ATC) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,619 T→A 17.0% M126L (ATG→TTG) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,623 G→C 16.9% V124V (GTC→GTG tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,626 T→G 12.7% A123A (GCA→GCC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,629 G→A 16.8% N122N (AAC→AAT tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,634 C→T 14.5% A121T (GCC→ACC)  tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,635 A→G 15.7% I120I (ATT→ATC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,638 A→G 15.7% G119G (GGT→GGC tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,641 C→T 14.9% Q118Q (CAG→CAA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,647 C→T 13.6% L116L (CTG→CTA) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,649 G→A 14.1% L116L (CTG→TTG) ‡ tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,653 A→T 12.8% G114G (GGT→GGA tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,659 G→C 8.0% T112T (ACC→ACG tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,074,662 G→C 7.8% T111T (ACC→ACG tktA ← transketolase 1, thiamine triphosphate‑binding
RA 3,303,385 G→T 8.9% N381K (AAC→AAA pnp ← polynucleotide phosphorylase/polyadenylase
RA 3,433,446 C→A 100% G315C (GGC→TGC)  rpoA ← RNA polymerase, alpha subunit
RA 3,553,857 G→A 8.6% R37C (CGC→TGC)  glpR ← GlpR DNA‑binding transcriptional repressor
RA 3,729,296 A→C 100% K320Q (AAG→CAG)  xylR → xylose divergent operon transcriptional activator
JC JC 4,053,617 IS4 (+) +12 bp 18.1% intergenic (+27/‑179) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator
RA 4,076,988 Δ1 bp 100% coding (2195/3051 nt) fdoG ← formate dehydrogenase‑O, large subunit
RA 4,097,480 Δ1 bp 100% coding (1/963 nt) pfkA → 6‑phosphofructokinase I
MC JC 4,097,486 Δ962 bp 100% [pfkA] [pfkA]
RA 4,141,257 T→G 15.8% A590A (GCA→GCC ppc ← phosphoenolpyruvate carboxylase
JC 4,224,391 Δ12 bp 47.2% coding (706‑717/1650 nt) pgi → glucosephosphate isomerase
MC JC 4,303,167 Δ450 bp 100% coding (1‑450/450 nt) rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
JC 4,629,587 +ATT 5.7% coding (537/717 nt) arcA ← response regulator in two‑component regulatory system with ArcB or CpxA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 1308899 1308955 57 30 [27] [0] 70 [ompW] [ompW]
* * ÷ CP009273 1857028 1858030 1003 63 [0] [0] 63 [gapA] [gapA]
* * ÷ CP009273 3099976 3100015 40 34 [27] [27] 30 nupG nucleoside transporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 15387 =NA (NA)7 (0.110) 7/268 NT 28.0% noncoding (1/1345 nt) IS186 repeat region
?CP009273 = 454251 18 (0.290)intergenic (+95/‑93) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
* ? CP009273 320127 =33 (0.510)30 (0.470) 29/276 NT 47.6% coding (659/696 nt) ykgG LutC family protein; putative electron transport chain YkgEFG component
?CP009273 376716 = NA (NA)intergenic (‑1/‑91) yaiX/insC1 pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase/IS2 repressor TnpA
* ? CP009273 = 32013133 (0.510)21 (0.330) 18/276 NT 38.9% coding (663/696 nt) ykgG LutC family protein; putative electron transport chain YkgEFG component
?CP009273 = 378046 NA (NA)intergenic (+11/+1) insD1/yaiX IS2 transposase TnpB/pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase
* ? CP009273 360104 =45 (0.700)62 (0.970) 49/276 NT 57.9% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 360180 =30 (0.470)41 (1.160)
+62 bp
37/152 NT 83.2% intergenic (‑114/+481) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 1308956 = 0 (0.000)intergenic (+41/+19) ompW/yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
* ? CP009273 454243 =18 (0.280)12 (0.190) 12/270 NT 40.5% intergenic (+87/‑101) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?CP009273 = 604803 NA (NA)intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? CP009273 454244 =18 (0.280)51 (0.820) 46/268 NT 74.5% intergenic (+88/‑100) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?CP009273 603467 = NA (NA)intergenic (+23/‑54) hokE/insL1 toxic polypeptide, small/IS186 transposase
* ? CP009273 = 45425518 (0.280)36 (0.580) 34/268 NT 67.3% intergenic (+99/‑89) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?CP009273 = 604803 NA (NA)intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? CP009273 = 604803NA (NA)7 (0.110) 7/270 NT 12.1% intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
?CP009273 1297568 = 52 (0.810)coding (413/921 nt) oppB oligopeptide transporter subunit
* ? CP009273 1092325 =72 (1.120)4 (0.060) 4/276 NT 5.3% intergenic (+40/+696) ycdU/serX putative inner membrane protein/tRNA‑Ser
?CP009273 = 1463497 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? CP009273 = 109233070 (1.090)11 (0.170) 11/274 NT 13.7% intergenic (+45/+691) ycdU/serX putative inner membrane protein/tRNA‑Ser
?CP009273 = 1645804 NA (NA)intergenic (+10/‑4) insD1/intQ IS2 transposase TnpB;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/pseudogene, Qin prophage; phage integrase family;Phage or Prophage Related
* ? CP009273 1203246 =10 (0.160)11 (0.190) 10/244 NT 52.6% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 11 (0.190)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326110 (0.160)15 (0.260) 13/244 NT 60.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 11 (0.190)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 129758054 (0.840)13 (0.210) 13/268 NT 19.9% coding (425/921 nt) oppB oligopeptide transporter subunit
?CP009273 2507756 = NA (NA)intergenic (+33/‑54) nupC/insL1 nucleoside (except guanosine) transporter/IS186 transposase
* ? CP009273 1342452 =57 (0.890)4 (0.070) 4/262 NT 6.7% coding (718/1935 nt) rnb ribonuclease II
?CP009273 = 1663663 57 (0.930)coding (187/894 nt) ynfL LysR family putative transcriptional regulator
* ? CP009273 = 1463497NA (NA)4 (0.060) 4/272 NT 6.7% noncoding (1/1331 nt) IS2 repeat region
?CP009273 1747090 = 56 (0.880)coding (998/2103 nt) ydhV putative oxidoreductase subunit
* ? CP009273 1483968 =NA (NA)55 (0.860) 46/276 NT 100% intergenic (+40/+2) aldA/gapC aldehyde dehydrogenase A, NAD‑linked/pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)
?CP009273 1858031 = 0 (0.000)intergenic (+8/‑76) gapA/yeaD glyceraldehyde‑3‑phosphate dehydrogenase A/D‑hexose‑6‑phosphate epimerase‑like protein
* ? CP009273 = 148508557 (0.890)38 (0.960)
+53 bp
32/170 NT 68.4% intergenic (‑115/‑74) gapC/cybB pseudogene, GAP dehydrogenase; glyceraldehyde‑3‑phosphate dehydrogenase (second fragment)/cytochrome b561
?CP009273 = 1857027 0 (0.000)intergenic (‑341/‑1) msrB/gapA methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A
* ? CP009273 1551001 =55 (0.860)186 (2.920) 126/274 NT 76.8% coding (120/432 nt) osmC lipoyl‑dependent Cys‑based peroxidase, hydroperoxide resistance; salt‑shock inducible membrane protein; peroxiredoxin
?CP009273 = 1558191 58 (0.910)coding (1800/2400 nt) dosP oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
* ? CP009273 3052022 =6 (0.090)645 (10.190)
+AG
176/272 NT 99.1% intergenic (‑253/+3) serA/rpiA D‑3‑phosphoglycerate dehydrogenase/ribose 5‑phosphate isomerase, constitutive
?CP009273 4161679 = NA (NA)noncoding (115/120 nt) rrfB 5S ribosomal RNA of rrnB operon
* ? CP009273 3060603 =48 (0.750)164 (2.550) 125/276 NT 77.4% intergenic (‑71/+96) ygfI/yggE putative DNA‑binding transcriptional regulator/oxidative stress defense protein
?CP009273 = 3124699 NA (NA)intergenic (+146/+1) insH1/yghQ IS5 transposase and trans‑activator/putative inner membrane polysaccharide flippase
* ? CP009273 3179455 =NA (NA)92 (1.430) 76/276 NT 100% intergenic (+7/‑91) yqiG/insC1 pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein/IS2 repressor TnpA
?CP009273 = 3426721 0 (0.000)coding (199/1125 nt) smf DNA recombination‑mediator A family protein
* ? CP009273 = 3180785NA (NA)65 (1.010) 56/276 NT 100% pseudogene (6/2445 nt) yqiG pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein
?CP009273 3426717 = 0 (0.000)coding (203/1125 nt) smf DNA recombination‑mediator A family protein
* ? CP009273 4532499 =65 (1.010)3 (0.050) 3/258 NT 5.1% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 4532813 = 50 (0.830)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? CP009273 = 453250765 (1.010)3 (0.050) 3/258 NT 5.1% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 = 4532803 50 (0.830)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)