Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F41 I0 R1	212	10.4	395026	92.3%	364608	148.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	8,810	T→C	81.4%	D191D (GAT→GAC)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	8,810	0	T	C	81.4%	156.5 / 25.1	69	D191D (GAT→GAC)	talB	transaldolase B
Reads supporting (aligned to +/- strand): ref base T (8/5); new base C (32/24); total (40/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGA  >  CP009273/8668‑8956

aaCAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATaaga                                                                                                                                                >  2:5890/1‑147 (MQ=255)

    gCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTa                                                                                                                                         <  2:19050/150‑1 (MQ=255)

        gAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGca                                                                                                                                     >  2:80813/1‑149 (MQ=255)

        gAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGca                                                                                                                                     <  1:134773/150‑2 (MQ=255)

         aaaaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGcac                                                                                                                                    >  1:52724/1‑148 (MQ=255)

          aaaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCAcc                                                                                                                                   >  2:191553/1‑150 (MQ=255)

           aaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCg                                                                                                                                  >  1:67635/1‑150 (MQ=255)

             agaagGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCgg                                                                                                                                 >  2:49892/1‑149 (MQ=255)

                 ggCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCag                                                                                                                              >  1:107487/1‑148 (MQ=255)

                   cATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCagaaga                                                                                                                          <  1:191553/150‑1 (MQ=255)

                     ttaaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATc                                                                                                                        <  2:194250/148‑1 (MQ=255)

                     tCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATc                                                                                                                        <  1:38708/150‑1 (MQ=255)

                         cTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCggg                                                                                                                    >  2:192567/1‑150 (MQ=255)

                         cTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCggg                                                                                                                    <  1:158248/150‑1 (MQ=255)

                               ccTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGAGTGGTACAAAGCCAACTCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGt                                                                                                              <  1:179167/150‑1 (MQ=255)

                                cTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGtt                                                                                                             <  1:8436/150‑1 (MQ=255)

                                cTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGtt                                                                                                             >  1:168965/1‑150 (MQ=255)

                                 tGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGttt                                                                                                            >  2:62865/1‑150 (MQ=255)

                                 tGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGttt                                                                                                            >  1:172228/1‑150 (MQ=255)

                                     ccTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGt                                                                                                        <  1:101798/150‑1 (MQ=255)

                                      ctgctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGTGCGTGGTTTCCGtc                                                                                                       <  2:145374/150‑2 (MQ=255)

                                      ctgctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtc                                                                                                       <  1:49892/150‑2 (MQ=255)

                                       tgctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGCTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGt                                                                                                        >  1:149479/1‑148 (MQ=255)

                                            ttCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGa                                                                                                  >  2:160980/1‑149 (MQ=255)

                                                 cTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTa                                                                                            <  1:160980/150‑1 (MQ=255)

                                                  ttCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTAc                                                                                           >  1:62505/1‑150 (MQ=255)

                                                      cTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGt                                                                                       <  1:142050/150‑1 (MQ=255)

                                                       tCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGta                                                                                      >  1:142210/1‑150 (MQ=255)

                                                            cTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGtactac                                                                                  >  2:147552/1‑149 (MQ=255)

                                                              cgcgCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACaag                                                                               >  1:124849/4‑149 (MQ=255)

                                                                cgCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACaag                                                                               >  2:162050/2‑147 (MQ=255)

                                                                 gCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACaagcag                                                                            <  1:162850/150‑5 (MQ=255)

                                                                      cgccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACgg                                                                       <  2:140583/146‑1 (MQ=255)

                                                                        ccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACggct                                                                     <  1:62865/148‑3 (MQ=255)

                                                                         cgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTa                                                                    >  1:148319/2‑150 (MQ=255)

                                                                          gAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGCAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGCCAGCGAACTCTACGAGTATTACAAGCAGCACGGCTAt                                                                   >  1:28535/1‑150 (MQ=255)

                                                                               gggCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCTGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAAc                                                              <  2:168965/150‑1 (MQ=255)

                                                                                 gCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCg                                                            <  2:126244/150‑1 (MQ=255)

                                                                                   gtgtTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTg                                                          >  1:167824/1‑150 (MQ=255)

                                                                                   gtgtTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTGCTGTATCTGAAATCTACCGGAAGAAGAAAGAGAACGGATAGGAAACCGTg                                                          >  1:53826/1‑150 (MQ=255)

                                                                                   gtcttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGtc                                                          >  1:19165/4‑149 (MQ=255)

                                                                                     gtTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGAACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGt                                                        >  1:35894/1‑150 (MQ=255)

                                                                                            atttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATgg                                                   <  2:38725/145‑1 (MQ=255)

                                                                                              ttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGgcg                                                >  2:173326/2‑149 (MQ=255)

                                                                                              ttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGgcg                                                >  1:156859/2‑149 (MQ=255)

                                                                                                cGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAACGAGCACGGTTATGAAAACGTGGTTATGGgcgc                                               >  1:13499/1‑148 (MQ=255)

                                                                                                   cgttcgttGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCt                                          <  2:145644/150‑1 (MQ=255)

                                                                                                   cgttcgttGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCt                                          >  2:54252/1‑150 (MQ=255)

                                                                                                   cgttcgttGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGAGCAAGCt                                          >  1:56432/1‑150 (MQ=255)

                                                                                                    gTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCtt                                         <  1:192567/150‑1 (MQ=255)

                                                                                                     ttcgttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTc                                        <  1:122670/147‑1 (MQ=255)

                                                                                                       cgttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTcc                                       <  2:47141/148‑1 (MQ=255)

                                                                                                           ggCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAc                                  >  1:62989/1‑150 (MQ=255)

                                                                                                            gCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcg                                 >  1:7906/1‑149 (MQ=255)

                                                                                                             ccGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgt                                >  1:157449/1‑148 (MQ=255)

                                                                                                             ccGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgt                                >  2:165241/1‑148 (MQ=255)

                                                                                                             ccGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgt                                >  1:48293/1‑148 (MQ=255)

                                                                                                              cGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgtt                               >  1:181283/1‑147 (MQ=255)

                                                                                                                 atcctcgACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGc                            >  2:139394/7‑150 (MQ=255)

                                                                                                                  tcctcgACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCg                           <  1:80813/145‑1 (MQ=255)

                                                                                                                            gTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGa                 <  1:115272/150‑1 (MQ=255)

                                                                                                                             tACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGaa                >  1:183717/1‑150 (MQ=255)

                                                                                                                               cAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACt              >  1:3713/1‑150 (MQ=255)

                                                                                                                                 aaGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTgg            <  1:54252/150‑1 (MQ=255)

                                                                                                                                     caacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAActggctgg        <  2:52724/146‑1 (MQ=255)

                                                                                                                                        tACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTg     >  2:132840/1‑150 (MQ=255)

                                                                                                                                          ccGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCGGGAACTGGCAGGCTGc    <  2:13499/149‑1 (MQ=255)

                                                                                                                                           cGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGa  <  2:65357/150‑1 (MQ=255)

                                                                                                                                           cGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTAGCGAAATTCTGGAACTGGCTGGCTGTGa  >  2:27188/1‑150 (MQ=255)

AACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGA  >  CP009273/8668‑8956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGG  >  CP009273/8675‑8946

GGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCA                                                                                                                           <  SRR12117902.137230/151‑1 (MQ=60)

  AAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACC                                                                                                                         >  SRR12117902.53683/1‑151 (MQ=60)

    AAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGG                                                                                                                       >  SRR12117902.68853/1‑151 (MQ=60)

          GGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGA                                                                                                                   >  SRR12117902.109447/1‑149 (MQ=60)

           GCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGA                                                                                                                <  SRR12117902.195034/151‑1 (MQ=60)

             ATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATC                                                                                                              <  SRR12117902.39427/151‑1 (MQ=60)

                 ACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGG                                                                                                          <  SRR12117902.161135/151‑1 (MQ=60)

                       ACCTGAGGCTGCTGTTCTCCTTCGCTCAGGCACGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGAGTGGTACAAAGCCAACTCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGT                                                                                                    <  SRR12117902.182408/151‑1 (MQ=60)

                        CCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTT                                                                                                   <  SRR12117902.8591/151‑1 (MQ=60)

                         CTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTT                                                                                                  >  SRR12117902.172055/1‑151 (MQ=60)

                          TGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTC                                                                                                 >  SRR12117902.175353/1‑151 (MQ=60)

                             CCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGT                                                                                              <  SRR12117902.103654/151‑1 (MQ=60)

                              GCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtc                                                                                             <  SRR12117902.50811/151‑5 (MQ=60)

                                TGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGCTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtc                                                                                             >  SRR12117902.152197/1‑145 (MQ=60)

                                         CCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTA                                                                                  <  SRR12117902.163914/151‑1 (MQ=60)

                                           TTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacg                                                                                >  SRR12117902.63642/1‑134 (MQ=60)

                                              GCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagt                                                                             <  SRR12117902.144632/151‑21 (MQ=60)

                                                TCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtat                                                                           >  SRR12117902.144796/1‑129 (MQ=60)

                                                       CGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtattacaagc                                                                    >  SRR12117902.127127/1‑122 (MQ=60)

                                                         cgcttgcgccGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtattacaagcag                                                                  <  SRR12117902.165827/141‑32 (MQ=60)

                                                                GCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtattacaagcagcacggct                                                           <  SRR12117902.64004/151‑39 (MQ=60)

                                                                  CGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtattacaagcagcacggctat                                                         >  SRR12117902.151021/1‑111 (MQ=60)

                                                                   GAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGCAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgccagcgaactctacgagtattacaagcagcacggctatg                                                        >  SRR12117902.29042/1‑110 (MQ=60)

                                                                            GTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGG                                               >  SRR12117902.170903/1‑151 (MQ=60)

                                                                            GTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTGCTGTATCTGAAATCTACCggaagaagaaagagaacggataggaaaccgtgg                                               >  SRR12117902.54809/1‑118 (MQ=60)

                                                                            GTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCcgtcagcgaaatctacgagtattacaagcagcacggctatgaaaccgtcg                                               >  SRR12117902.19490/1‑101 (MQ=60)

                                                                              GTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGAACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTT                                             >  SRR12117902.36543/1‑151 (MQ=60)

                                                                                       TTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGC                                     >  SRR12117902.159718/1‑150 (MQ=44)

                                                                                         CGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAACGAGCACGGCTATGAAAACGTGGTTATGGGCGCA                                    >  SRR12117902.13718/1‑149 (MQ=60)

                                                                                            CGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTT                               <  SRR12117902.196060/151‑1 (MQ=47)

                                                                                            CGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGAGCAAGCTT                               >  SRR12117902.57462/1‑151 (MQ=39)

                                                                                             GTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTC                              <  SRR12117902.124908/151‑1 (MQ=48)

                                                                                                    GGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACG                       >  SRR12117902.64128/1‑151 (MQ=53)

                                                                                                     GCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGT                      >  SRR12117902.8054/1‑151 (MQ=51)

                                                                                                      CCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACgtt                     >  SRR12117902.49185/1‑148 (MQ=50)

                                                                                                      CCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACgtt                     >  SRR12117902.160320/1‑148 (MQ=50)

                                                                                                       CGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACgttg                    >  SRR12117902.184564/1‑147 (MQ=51)

                                                                                                          ATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCG                 <  SRR12117902.82279/151‑1 (MQ=50)

                                                                                                                    GGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGA       <  SRR12117902.117369/151‑1 (MQ=54)

                                                                                                                      TACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAAC     >  SRR12117902.187068/1‑151 (MQ=54)

                                                                                                                        CAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTG   >  SRR12117902.3764/1‑151 (MQ=54)

                                                                                                                         AAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGG  <  SRR12117902.55250/151‑1 (MQ=56)

GGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGG  >  CP009273/8675‑8946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`