Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F15 I1 R1 782 634.5 21863942 98.1% 21448527 138.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,269,697 G→A G200S (GGT→AGT)  yhaC → YhaC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,269,6970GA100.0% 488.3 / NA 129G200S (GGT→AGT) yhaCYhaC family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (62/67);  total (62/67)

Only 100 of 129 total aligned reads displayed.

CCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACAT  >  NZ_CP025268/3269560‑3269830
                                                                                                                                         |                                                                                                                                     
    tctGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCaa                                                                                                                                 >  1:10174121/1‑140 (MQ=255)
    tctGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCaa                                                                                                                                 >  1:4921836/1‑140 (MQ=255)
    tctGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCaa                                                                                                                                 >  1:2416418/1‑140 (MQ=255)
      tGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAaca                                                                                                                               <  1:5475018/140‑1 (MQ=255)
           ttACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACaaaa                                                                                                                          <  2:6121441/140‑1 (MQ=255)
             aCGTAATGTCGATTTAGATTATTATGATTTCACATATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACg                                                                                                                        >  1:3836645/1‑140 (MQ=255)
             aCGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACg                                                                                                                        >  1:9207521/1‑140 (MQ=255)
             aCGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACg                                                                                                                        >  2:7695812/1‑140 (MQ=255)
                  aTGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCcaca                                                                                                                   <  2:9307822/140‑1 (MQ=255)
                  aTGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCcaca                                                                                                                   <  1:1604181/140‑1 (MQ=255)
                  aTGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCcaca                                                                                                                   <  1:2864403/140‑1 (MQ=255)
                  aTGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCcaca                                                                                                                   <  1:4755741/140‑1 (MQ=255)
                  aTGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCcaca                                                                                                                   >  2:4535724/1‑140 (MQ=255)
                  aTGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAAAGCcaca                                                                                                                   >  2:9263335/1‑140 (MQ=255)
                   tGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACaa                                                                                                                  <  1:1335390/140‑1 (MQ=255)
                   tGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACaa                                                                                                                  <  1:1335316/140‑1 (MQ=255)
                                       aTTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCCACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTa                                                                                              >  2:7713263/1‑140 (MQ=255)
                                        tttCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCca                                                                                                                     >  2:1852602/1‑116 (MQ=255)
                                        tttCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCca                                                                                                                     <  1:1852602/116‑1 (MQ=255)
                                            acaGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAAt                                                                                         >  1:3846221/1‑140 (MQ=255)
                                               gATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAAc                                                                                      >  2:6680143/1‑140 (MQ=255)
                                                aTAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACg                                                                                     <  2:2058493/140‑1 (MQ=255)
                                                           gCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaat                                                                          <  1:6225568/140‑1 (MQ=255)
                                                              aaTACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAg                                                                       <  1:5221410/140‑1 (MQ=255)
                                                               aTACTATTTTAAATCCTTTTAGTTTGACTTCTAGCAATTTTACTAATGCGATCTTGTTTCAGGTGAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGCTTATGTACTAAGAATACCGACTGTGTTGATAAGc                                                                      >  2:8748171/1‑140 (MQ=255)
                                                               aTACTATTTTAAATCATTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCTGGTTAATTTTGTTAGTTCAACGCAAAACGCCACAATCTACTGGGATTATTTACTAAAAATAAAGCCTGTGTTAATAAGc                                                                      >  1:10725129/1‑140 (MQ=255)
                                                                 aCTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCAt                                                                    <  2:10174121/140‑1 (MQ=255)
                                                                 aCTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTACAACACAAACCGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCAt                                                                    <  1:10786063/140‑1 (MQ=255)
                                                                   tATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTa                                                                  <  1:1086805/140‑1 (MQ=255)
                                                                     ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                                <  1:8749022/140‑1 (MQ=255)
                                                                     ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                                >  2:10076262/1‑140 (MQ=255)
                                                                     ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                                >  1:7342428/1‑140 (MQ=255)
                                                                     ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                                <  1:9877311/140‑1 (MQ=255)
                                                                     ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                                >  2:3804069/1‑140 (MQ=255)
                                                                     ttttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                                <  2:5395206/140‑1 (MQ=255)
                                                                     ttttAAATCCTTTTAAATTGAATTCAACAAAGTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                                <  2:9182550/140‑1 (MQ=255)
                                                                      tttAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCg                                                               <  1:6367066/140‑1 (MQ=255)
                                                                        tAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGTTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGat                                                             <  2:3836645/140‑1 (MQ=255)
                                                                        tAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGat                                                             <  2:9207521/140‑1 (MQ=255)
                                                                             ccTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCg                                                               <  2:9192255/133‑1 (MQ=255)
                                                                             ccTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCg                                                               >  1:9192255/1‑133 (MQ=255)
                                                                             ccTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTa                                                        <  2:3495240/140‑1 (MQ=255)
                                                                               ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTAtt                                                      >  2:3671345/1‑140 (MQ=255)
                                                                               ttttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTAtt                                                      >  2:2400373/1‑140 (MQ=255)
                                                                                 ttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCt                                                    >  1:3610326/1‑140 (MQ=255)
                                                                                 ttAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCt                                                    >  1:4689343/1‑140 (MQ=255)
                                                                                         aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTAttt                                                                                                  <  2:4656892/86‑1 (MQ=255)
                                                                                         aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTAttt                                                                                                  >  1:4656892/1‑86 (MQ=255)
                                                                                         aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                                >  2:1357718/1‑120 (MQ=255)
                                                                                         aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGaaaa                                            >  2:10906130/1‑140 (MQ=255)
                                                                                         aaTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGaaaa                                            >  1:2121034/1‑140 (MQ=255)
                                                                                          aTTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGaaaaa                                           >  1:8553543/1‑140 (MQ=255)
                                                                                                   aTTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTg                                                                                                           >  2:2719380/1‑67 (MQ=255)
                                                                                                        aCTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCca                                                                                                                     <  2:844265/52‑1 (MQ=255)
                                                                                                        aCTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCca                                                                                                                     >  1:844215/1‑52 (MQ=255)
                                                                                                        aCTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                                >  1:5071755/1‑105 (MQ=255)
                                                                                                        aCTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                                <  2:5071755/105‑1 (MQ=255)
                                                                                                        aCTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGa                             >  1:2151053/1‑140 (MQ=255)
                                                                                                           aaTGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACaaaa                                                                                                                          >  2:4459472/1‑44 (MQ=255)
                                                                                                           aaTGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                          >  1:736532/1‑140 (MQ=255)
                                                                                                           aaTGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                          <  1:3804069/140‑1 (MQ=255)
                                                                                                            aTGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                    >  2:7744173/1‑81 (MQ=255)
                                                                                                            aTGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGc                                                                                    <  1:7744173/81‑1 (MQ=255)
                                                                                                             tGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTgaagaa                                              <  1:9192889/118‑1 (MQ=255)
                                                                                                             tGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAgtt                        >  2:4844890/1‑140 (MQ=255)
                                                                                                             tGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAgtt                        >  2:5404729/1‑140 (MQ=255)
                                                                                                              gCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaat                                                                          <  1:5463882/89‑1 (MQ=255)
                                                                                                              gCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTtaat                                                                          >  2:5463882/1‑89 (MQ=255)
                                                                                                               ccAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAgttgt                      <  2:6034555/140‑1 (MQ=255)
                                                                                                                    aTGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                 <  1:7713263/140‑1 (MQ=255)
                                                                                                                    aTGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                 <  2:8244650/140‑1 (MQ=255)
                                                                                                                    aTGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                 <  2:8553543/140‑1 (MQ=255)
                                                                                                                    aTGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                 <  2:4921836/140‑1 (MQ=255)
                                                                                                                    aTGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                 <  1:4535724/140‑1 (MQ=255)
                                                                                                                     tGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGTGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAAt                <  2:7953133/140‑1 (MQ=255)
                                                                                                                     tGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAAt                <  1:5555638/140‑1 (MQ=255)
                                                                                                                      gTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATg               <  2:5037861/140‑1 (MQ=255)
                                                                                                                      gTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATg               <  1:2948434/140‑1 (MQ=255)
                                                                                                                      gTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATg               <  1:7482880/140‑1 (MQ=255)
                                                                                                                        ttCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGc                                                                >  2:7090946/1‑89 (MQ=255)
                                                                                                                        ttCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                          >  1:7999291/1‑127 (MQ=255)
                                                                                                                        ttCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                          <  2:2488643/127‑1 (MQ=255)
                                                                                                                        ttCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                          <  2:7999291/127‑1 (MQ=255)
                                                                                                                        ttCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                          >  1:2488643/1‑127 (MQ=255)
                                                                                                                        ttCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAg             >  2:6077057/1‑140 (MQ=255)
                                                                                                                           aGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCt          <  1:10207365/140‑1 (MQ=255)
                                                                                                                            ggTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                 <  1:8617550/132‑1 (MQ=255)
                                                                                                                            ggTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                 >  2:8617550/1‑132 (MQ=255)
                                                                                                                             gTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTgg        >  1:9292235/1‑140 (MQ=255)
                                                                                                                             gTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTgg        >  2:2791339/1‑140 (MQ=255)
                                                                                                                              ttAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGga       <  2:2151053/140‑1 (MQ=255)
                                                                                                                                aaTTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGgaga     >  2:8795443/1‑140 (MQ=255)
                                                                                                                                 aTTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGAc    >  2:5811824/1‑140 (MQ=255)
                                                                                                                                 aTTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGAc    >  1:5506308/1‑140 (MQ=255)
                                                                                                                                  ttttGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                          <  1:3028203/117‑1 (MQ=255)
                                                                                                                                  ttttGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAg                          >  2:3028203/1‑117 (MQ=255)
                                                                                                                                  ttttGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                 <  2:7062018/126‑1 (MQ=255)
                                                                                                                                  ttttGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTaaa                 >  1:7062018/1‑126 (MQ=255)
                                                                                                                                  ttttGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACa   >  1:2343418/1‑140 (MQ=255)
                                                                                                                                   tttGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACAt  <  2:7057187/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
CCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACAT  >  NZ_CP025268/3269560‑3269830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGAAGAA  >  NZ_CP025268/3269549‑3269840
                                                                                                                                                    |                                                                                                                                               
CGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTAAGGTTAATTTTGTTAGT                                                                                                                                               >  SRR14920162.7622848/1‑151 (MQ=60)
    GATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAA                                                                                                                                           >  SRR14920162.10236173/1‑151 (MQ=60)
    GATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAA                                                                                                                                           >  SRR14920162.8981022/1‑151 (MQ=60)
    GATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAA                                                                                                                                           >  SRR14920162.4950723/1‑151 (MQ=60)
    GATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAA                                                                                                                                           >  SRR14920162.2430709/1‑151 (MQ=60)
       CTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACAC                                                                                                                                        <  SRR14920162.10226093/151‑1 (MQ=60)
          tttcacgcagacgacgccaaccgagattcgccttagtctcgtgggctcggagatgtgtataagagacagATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAA                                                                                                                                     <  SRR14920162.9343495/82‑1 (MQ=60)
          tttattgcatatttctgaatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacagATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAA                                                                                                                                     <  SRR14920162.9343506/82‑1 (MQ=60)
             TCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACATATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTAAACACAAAACG                                                                                                                                  >  SRR14920162.3859325/1‑151 (MQ=60)
             TCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACG                                                                                                                                  >  SRR14920162.9263515/1‑151 (MQ=60)
                 TGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCAC                                                                                                                              <  SRR14920162.5507267/151‑1 (MQ=60)
                      cccccccccccccccttaacacccaccacccgccaaacgagattcgccttagtctcgtgggctcggagatgtgtataagagacagCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCT                                                                                                                         <  SRR14920162.4485711/66‑1 (MQ=60)
                           gtataagagacagGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGG                                                                                                                    <  SRR14920162.1863310/138‑1 (MQ=60)
                             ATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGA                                                                                                                  <  SRR14920162.4783598/151‑1 (MQ=60)
                             ATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGA                                                                                                                  <  SRR14920162.2881297/151‑1 (MQ=60)
                             ATGTCGATTTAGATTATTATGATTTCAAAGATAAAAATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCTGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGA                                                                                                                  <  SRR14920162.1613457/151‑1 (MQ=60)
                              TGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGAT                                                                                                                 <  SRR14920162.1343110/151‑1 (MQ=60)
                              TGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGAT                                                                                                                 <  SRR14920162.1343185/151‑1 (MQ=60)
                                     cacaccccgccacccgcgattcgccttagtctcgtgggctcggagatgtgtataagagacagGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTAC                                                                                                          <  SRR14920162.2735425/89‑1 (MQ=60)
                                            ATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAAT                                                                                                   >  SRR14920162.3868955/1‑151 (MQ=60)
                                                            cgcgattcgccttagtctcgtgggctcggagatgtgtataagagacagAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATA                                                                                   <  SRR14920162.7790958/103‑1 (MQ=60)
                                                               ACATATGGCTAATACTATTTTAAATCATTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCTGGTTAATTTTGTTAGTTCAACGCAAAACGCCACAATCTACTGGGATTATTTACTAAAAATAAAGCCTGTGTTAATAAGC                                                                                >  SRR14920162.10791156/1‑151 (MQ=60)
                                                                     GGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGC                                                                          >  SRR14920162.7386662/1‑151 (MQ=60)
                                                                      gccttagtctcgtgggctcggagatgtgtataagagacagATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCG                                                                         <  SRR14920162.5496063/111‑1 (MQ=60)
                                                                      GCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCG                                                                         <  SRR14920162.6262595/151‑1 (MQ=60)
                                                                       ATAATAATATTTTAAATCATTTTAAATTTAATTCAACAAATTTTAATATTGCAAAAATTTTTCAGTTTAATTTTTTTAGTTAAACACAAAACGCCACAATCTCCTGGTATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGA                                                                        <  SRR14920162.2258230/151‑1 (MQ=60)
                                                                         AATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATA                                                                      <  SRR14920162.5252127/151‑1 (MQ=60)
                                                                            ACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTACAACACAAACCGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGT                                                                   <  SRR14920162.10852467/151‑1 (MQ=60)
                                                                             CTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTA                                                                  >  SRR14920162.9248159/1‑151 (MQ=60)
                                                                              cccgccaaccgcgattcgccttagtctcgtgggctcggagatgtgtataagagacagGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTAT                                                                 <  SRR14920162.318878/94‑1 (MQ=60)
                                                                              agaagccggaaaacgcgattcgccttagtctcgtgggctcggagatgtgtataagagacagAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTAT                                                                 <  SRR14920162.10719470/90‑1 (MQ=60)
                                                                              agaagacggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacagAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTAT                                                                 <  SRR14920162.10720118/90‑1 (MQ=60)
                                                                              TATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGTTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTAT                                                                 <  SRR14920162.1093065/151‑1 (MQ=60)
                                                                              TATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTAT                                                                 <  SRR14920162.1093196/151‑1 (MQ=60)
                                                                               ATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGcttattaaa                                                                >  SRR14920162.8208116/1‑142 (MQ=60)
                                                                                gccttagtctcgtgggctcggagatgtgtataagagacagTGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTC                                                               <  SRR14920162.7133585/111‑1 (MQ=60)
                                                                                aagagacagCTTTTAAATTGAATTCACCAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTC                                                               <  SRR14920162.1365622/142‑1 (MQ=60)
                                                                                TTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTC                                                               <  SRR14920162.8802267/151‑1 (MQ=60)
                                                                                TTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTC                                                               <  SRR14920162.9937508/151‑1 (MQ=60)
                                                                                 TTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCT                                                              >  SRR14920162.4716835/1‑151 (MQ=60)
                                                                                 TTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCT                                                              >  SRR14920162.3631713/1‑151 (MQ=60)
                                                                                 TTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCT                                                              <  SRR14920162.6405027/151‑1 (MQ=60)
                                                                                   TAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGA                                                            <  SRR14920162.9544967/151‑1 (MQ=60)
                                                                                         CTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACtgtctcttatacacatctccgagcccacgagactaaggcgaa                                                      >  SRR14920162.4684196/1‑109 (MQ=60)
                                                                                         CTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAA                                                      >  SRR14920162.2133512/1‑151 (MQ=60)
                                                                                          TTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAA                                                     >  SRR14920162.8605537/1‑151 (MQ=60)
                                                                                              AAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCA                                                 >  SRR14920162.6070326/1‑151 (MQ=60)
                                                                                                  ataagagacagAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTT                                             <  SRR14920162.9248803/140‑1 (MQ=60)
                                                                                                        CAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGctgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtttgccgggttgtgggtgtaaatgtgggg                                       >  SRR14920162.849148/1‑74 (MQ=60)
                                                                                                        CAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGctgtctcttatacacatctccgagcccacgagactaaggcgaatcgcggtttccggcttcggcgtggaaaagggggg                                       >  SRR14920162.849199/1‑74 (MQ=60)
                                                                                                        CAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCctgtctcttatacacatctccgag                                       >  SRR14920162.5101556/1‑127 (MQ=60)
                                                                                                        CAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGA                                       >  SRR14920162.2163729/1‑151 (MQ=60)
                                                                                                           CAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAG                                    >  SRR14920162.740851/1‑151 (MQ=60)
                                                                                                                      tataagagacaGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATG                         <  SRR14920162.3046163/140‑1 (MQ=60)
                                                                                                                      AATGCCAACATGTTTCAGTTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATG                         <  SRR14920162.3693102/151‑1 (MQ=60)
                                                                                                                      AATGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATG                         <  SRR14920162.3826564/151‑1 (MQ=60)
                                                                                                                        TGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGct                       >  SRR14920162.2503363/1‑149 (MQ=60)
                                                                                                                        TGCCAACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGct                       >  SRR14920162.8047864/1‑149 (MQ=60)
                                                                                                                             ACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCctgtctcttatacacatctccgagcccacgagactaagtcgaatctcggattgaggagtgagattttaaaatttgggggggggggggggggggggggggggggggggggg                  >  SRR14920162.3511822/1‑41 (MQ=60)
                                                                                                                             ACATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTATAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATAAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGG                  >  SRR14920162.9348759/1‑151 (MQ=60)
                                                                                                                               ATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAG                <  SRR14920162.4562373/151‑1 (MQ=60)
                                                                                                                               ATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAG                <  SRR14920162.7759858/151‑1 (MQ=60)
                                                                                                                               ATGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAG                <  SRR14920162.8669935/151‑1 (MQ=60)
                                                                                                                                ggagatgtgtataagagacaGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGA               <  SRR14920162.5698269/131‑1 (MQ=60)
                                                                                                                                TGTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGA               <  SRR14920162.5588357/151‑1 (MQ=60)
                                                                                                                                 GTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGAC              >  SRR14920162.5538744/1‑151 (MQ=60)
                                                                                                                                 GTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGAC              <  SRR14920162.2965853/151‑1 (MQ=60)
                                                                                                                                 GTTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGAC              <  SRR14920162.7528005/151‑1 (MQ=60)
                                                                                                                                  TTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGctg             >  SRR14920162.7104458/1‑148 (MQ=60)
                                                                                                                                  TTTCAGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACA             >  SRR14920162.2357222/1‑151 (MQ=60)
                                                                                                                                      AGGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTAC         <  SRR14920162.10269688/151‑1 (MQ=60)
                                                                                                                                       GGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACC        >  SRR14920162.5294248/1‑151 (MQ=60)
                                                                                                                                       GGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACC        >  SRR14920162.10679070/1‑151 (MQ=60)
                                                                                                                                       GGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACC        >  SRR14920162.10601355/1‑151 (MQ=60)
                                                                                                                                       GGTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGACTGGAGACATTACC        >  SRR14920162.5272936/1‑151 (MQ=60)
                                                                                                                                        GTTAATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCG       <  SRR14920162.6021596/151‑1 (MQ=60)
                                                                                                                                           AATTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCctgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtatgccgtcgtcggcttggaaagggggg    >  SRR14920162.7111634/1‑75 (MQ=60)
                                                                                                                                             TTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGAAGAA  <  SRR14920162.5436545/151‑1 (MQ=60)
                                                                                                                                             TTTTGTTAGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGAAGAA  <  SRR14920162.4873257/151‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                               
CGTTGATCTATCCTCTCTGGACTTACGTAATGTCGATTTAGATTATTATGATTTCACAGATAAACATATGGCTAATACTATTTTAAATCCTTTTAAATTGAATTCAACAAATTTTACTAATGCCAACATGTTTCAGGTTAATTTTGTTGGTTCAACACAAAACGCCACAATCTCCTGGGATTATTTACTAAAAATAACGCCTGTTTTAATAAGCATTAGCGATATGTATTCTGAAGAAAAAATCAAGTTTGTCGAAAGTTGTTTAAATGAGCCTGGAGACATTACCGAAGAA  >  NZ_CP025268/3269549‑3269840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑