Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I1 R1	782	316.7	10427156	97.0%	10114341	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,743,290	+C	intergenic (‑66/‑116)	yiaJ ← / → galK	IclR family transcriptional regulator YiaJ/galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,743,289	1	.	C	98.1%	345.8 / ‑0.4	107	intergenic (‑65/‑117)	yiaJ/galK	IclR family transcriptional regulator YiaJ/galactokinase
Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (60/45); total (60/47)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑A‑CAATTGATTTTTATGTCTTTTGAAATTCATCAATCCTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCAGGAGGCAGATCATGAGTCTGAAAGAAAAA  >  NZ_CP025268/3743151‑3743423

ctctGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑A‑c                                                                                                                                       <  2:432225/140‑1 (MQ=255)

   tGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑A‑ccaa                                                                                                                                    <  2:1001339/140‑4 (MQ=255)

    ggCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑A‑ccaat                                                                                                                                   <  1:2551188/140‑5 (MQ=255)

    ggCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTCATGGTAGCGCACAGTGTGCCGTAGTTCACGATCTCGACCGATAATTTAT‑C‑A‑ccaat                                                                                                                                   <  2:3726028/140‑5 (MQ=255)

    ggCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTGCTTTCCCCATTTTGTCGCGTCCTGATTGTCGCGCAAAGTGGGCCGTAGTTCACGCTCTCGACAGATAATTTAT‑A‑A‑ccaat                                                                                                                                   >  2:3122230/1‑136 (MQ=255)

         ccGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGAtt                                                                                                                               >  1:4732977/1‑140 (MQ=255)

           ggCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCCTCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAC‑A‑ACCAATTGAtttt                                                                                                                             >  1:416916/1‑140 (MQ=255)

           ggCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGAtttt                                                                                                                             >  1:416956/1‑140 (MQ=255)

           ggCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGAtttt                                                                                                                             >  1:416698/1‑140 (MQ=255)

               ggACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATg                                                                                                                         <  2:4824287/140‑1 (MQ=255)

                gACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGt                                                                                                                        >  2:2968453/1‑140 (MQ=255)

                 aCGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTc                                                                                                                       <  1:2968453/140‑1 (MQ=255)

                     tctTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCtttt                                                                                                                   >  2:2847436/1‑140 (MQ=255)

                        ttttCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGCTTTTTATGTCTTTTGaa                                                                                                                <  1:421231/140‑1 (MQ=255)

                        ttttCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGaa                                                                                                                <  1:3300455/140‑1 (MQ=255)

                               tcgcCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTTAGGATGGTAGCGTAAAGTATGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTGTGTCTTGTGAAATtcatc                                                                                                         >  2:2603043/2‑140 (MQ=255)

                               gcgcCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATtcatc                                                                                                         >  2:1073101/1‑140 (MQ=255)

                                     tctcGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTGGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATCGATTTTTATGTCTTTGGAAATTCATCAATCag                                                                                                   >  2:3495727‑M1/1‑138 (MQ=255)

                                     tctcGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCag                                                                                                   >  1:4072774‑M1/1‑138 (MQ=255)

                                      ctcGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGGGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTTATTTTTATGTCTTTTGAAATTCATCAATCaga                                                                                                  <  2:2981075‑M1/140‑4 (MQ=255)

                                          ttCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgc                                                                                              >  2:375154‑M1/1‑133 (MQ=255)

                                           tctcTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACCGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  2:4448069‑M1/140‑9 (MQ=255)

                                           tctcTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑ACC‑CAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  2:1436871‑M1/140‑9 (MQ=255)

                                           tctcTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGACATTCATCAATCagattgcc                                                                                             <  2:306434‑M1/140‑9 (MQ=255)

                                           tctcTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  2:420815‑M1/140‑9 (MQ=255)

                                           tctcTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  1:273409‑M1/140‑9 (MQ=255)

                                           tctcTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  1:1688417‑M1/140‑9 (MQ=255)

                                           tctcTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  1:273120‑M1/140‑9 (MQ=255)

                                           tctcTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  1:3886608‑M1/140‑9 (MQ=255)

                                           tctcTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  2:420743‑M1/140‑9 (MQ=255)

                                           tctcTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  1:3488619‑M1/140‑9 (MQ=255)

                                           tctcTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  1:3732944‑M1/140‑9 (MQ=255)

                                           tctcTTTGTTTCCCCTCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTTGTTCACGATCTCGACAGATAATTTAT‑A‑ACCTTTTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  2:1204587‑M1/140‑9 (MQ=255)

                                               tttttttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgt                                                                                         >  2:1315024‑M1/1‑128 (MQ=255)

                                               tttttttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgt                                                                                         >  2:1315811‑M1/1‑128 (MQ=255)

                                               tttttttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgt                                                                                         >  1:1180148‑M1/1‑128 (MQ=255)

                                                   tttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaa                                                                                     <  2:1016377‑M1/140‑17 (MQ=255)

                                                   tttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaa                                                                                     <  2:1016475‑M1/140‑17 (MQ=255)

                                                     tCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaa                                                                                   <  1:441859‑M1/140‑19 (MQ=255)

                                                         aTCACTTCTTTCCCCATTTTGTCGCCTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtga                                                                               >  2:4661711‑M1/1‑118 (MQ=255)

                                                          tCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgat                                                                              <  1:2830690‑M1/140‑24 (MQ=255)

                                                          tCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgat                                                                              <  2:4046293‑M1/140‑24 (MQ=255)

                                                          tCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgat                                                                              <  2:4046337‑M1/140‑24 (MQ=255)

                                                          tCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgat                                                                              <  2:2820630‑M1/140‑24 (MQ=255)

                                                          tCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgat                                                                              <  1:2112725‑M1/140‑24 (MQ=255)

                                                                        aTTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGCTTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgctcgatatatttgaaa                                                                >  2:3312452‑M1/1‑103 (MQ=255)

                                                                        aTTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaa                                                                >  1:649955‑M1/1‑103 (MQ=255)

                                                                        aTTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATGTTTATGTCTTTTGAAATTCATCAATAagattcccttgttaaaaattgatcgatatatttgaaa                                                                >  1:4041084‑M1/1‑103 (MQ=255)

                                                                          tttGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatg                                                              >  1:2694878‑M1/1‑101 (MQ=255)

                                                                           ttGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatca                                                             >  2:4942961‑M1/1‑100 (MQ=255)

                                                                             gTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgtgaaaaagtgatcgatatatttgaaatcaag                                                           >  1:2453829‑M1/1‑98 (MQ=255)

                                                                               cgcgTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtt                                                         <  2:2187975‑M1/140‑45 (MQ=255)

                                                                               cgcgTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtt                                                         >  1:2351203‑M1/1‑96 (MQ=255)

                                                                                   tCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgc                                                     >  2:96708‑M1/1‑92 (MQ=255)

                                                                                   tCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgc                                                     >  1:4344292‑M1/1‑92 (MQ=255)

                                                                                        aTGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatatt                                                >  1:37497‑M1/1‑87 (MQ=255)

                                                                                            tAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaa                                            >  1:563441‑M1/1‑83 (MQ=255)

                                                                                            tAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaa                                            >  1:563890‑M1/1‑83 (MQ=255)

                                                                                             aGCGCAAAGTGTGCCGTAGTTCACGATGTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtggtcgatatatttgaaatcaagtttcgcatattgaaat                                           >  2:1094931‑M1/1‑82 (MQ=255)

                                                                                             aGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgtcttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaat                                           >  2:1567510‑M1/1‑82 (MQ=255)

                                                                                              gcgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaatt                                          >  2:3083021‑M1/1‑81 (MQ=255)

                                                                                              gcgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaatt                                          >  1:1642672‑M1/1‑81 (MQ=255)

                                                                                              gcgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaatt                                          >  1:2546050‑M1/1‑81 (MQ=255)

                                                                                              gcgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaatt                                          >  1:3192332‑M1/1‑81 (MQ=255)

                                                                                               cgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattt                                         >  1:1457406‑M1/1‑80 (MQ=255)

                                                                                               cgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattt                                         >  1:858152‑M1/1‑80 (MQ=255)

                                                                                               cgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             <  2:2365037‑M1/88‑9 (MQ=255)

                                                                                               cgcAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             >  1:2365037‑M1/1‑80 (MQ=255)

                                                                                                 ccaagtGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgctgtatttgaaatcaagtttcgcatattgaaatttta                                       <  1:4537782‑M1/138‑63 (MQ=255)

                                                                                                      tgtgCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagcca                                  >  2:431082‑M1/1‑73 (MQ=255)

                                                                                                        tgCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaa                                >  1:1003319‑M1/1‑71 (MQ=255)

                                                                                                        tgCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaa                                >  1:1003321‑M1/1‑71 (MQ=255)

                                                                                                        tgCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgaatattgaaattttaagccaaa                                >  1:1299919‑M1/1‑71 (MQ=255)

                                                                                                          ccGTAGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaa                              >  2:1924998‑M1/1‑69 (MQ=255)

                                                                                                              aGTTCACGATCTCGCCAGATAATTTAT‑A‑TCCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcg                          <  2:1190528‑M1/140‑76 (MQ=255)

                                                                                                              aGTTCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcg                          >  2:4861725‑M1/1‑65 (MQ=255)

                                                                                                              aGTGCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcg                          <  1:3508970‑M1/140‑76 (MQ=255)

                                                                                                                ttCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCGTCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgat                        >  2:96021‑M1/1‑63 (MQ=255)

                                                                                                                ttCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATTagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgat                        >  1:4864162‑M1/1‑63 (MQ=255)

                                                                                                                ttCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgat                        >  2:1215783‑M1/1‑63 (MQ=255)

                                                                                                                ttCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgat                        >  2:4599313‑M1/1‑63 (MQ=255)

                                                                                                                 tCACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatc                       <  2:2546050‑M1/140‑79 (MQ=255)

                                                                                                                  cACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatca                      >  2:1161162‑M1/1‑61 (MQ=255)

                                                                                                                  cACGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTATGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccgaaaaagcgatca                      >  1:4438899‑M1/1‑61 (MQ=255)

                                                                                                                   aagagctcgacagATAATTTATAA‑A‑CAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatctttgaaatcaagtttcgcatattgaaattttaagccaaaaaagctatcaa                     <  2:2396417‑M1/135‑81 (MQ=255)

                                                                                                                   aCGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaa                     <  1:3808801‑M1/140‑81 (MQ=255)

                                                                                                                   aCGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaa                     <  1:1294604‑M1/140‑81 (MQ=255)

                                                                                                                   aCGATCTCGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGACTTTTGAAATTCATCAATCagattgcattgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaa                     <  2:2396716‑M1/140‑81 (MQ=255)

                                                                                                                       tctcGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagcttgccttgttaaaaagtgatcgatatatttgaaatcaactttcgcatattgaaattgtatgccaaaaaaacgatcaaaaaa                 >  2:1044114‑M1/1‑56 (MQ=255)

                                                                                                                       tctcGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaaaaaa                 >  2:1042103‑M1/1‑56 (MQ=255)

                                                                                                                       tctcGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgcc                                                                                             >  1:1857655‑M1/1‑56 (MQ=255)

                                                                                                                          cGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaaaaaaaca              >  2:1410831‑M1/1‑53 (MQ=255)

                                                                                                                          cGACAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaaaaaaaca              >  1:4883645‑M1/1‑53 (MQ=255)

                                                                                                                            aCAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaaaaaaacaag            <  1:2802346‑M1/140‑90 (MQ=255)

                                                                                                                            aCAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaaaaaaacaag            <  1:723161‑M1/140‑90 (MQ=255)

                                                                                                                            aCAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgagcaaaaaaacaag            <  2:5131939‑M1/140‑90 (MQ=255)

                                                                                                                             cAGATAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaaaaaaacaagg           <  2:352558‑M1/140‑91 (MQ=255)

                                                                                                                                aTAATTTAT‑A‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatattttaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaaaaaaacaaggaag        >  2:5041577‑M1/1‑47 (MQ=255)

                                                                                                                                  aaTTTAT‑A‑ACCAATTGCTTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttacaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaaaaaaacaaggaagac      <  1:2887128‑M1/140‑96 (MQ=255)

                                                                                                                                      tat‑a‑ACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaaaaagcgatcaaaaaaacaaggaagcctggg  <  1:730072‑M1/140‑100 (MQ=255)

CTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTAT‑A‑A‑CAATTGATTTTTATGTCTTTTGAAATTCATCAATCCTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCAGGAGGCAGATCATGAGTCTGAAAGAAAAA  >  NZ_CP025268/3743151‑3743423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCGAAACAGACTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCATCAATCCTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCAGGAGGCAGATCATG  >  NZ_CP025268/3743140‑3743408

CGCGAAACAGACTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑C                                                                                                                        <  SRR14920191.1894455/151‑1 (MQ=60)

CGCGAAACAGACTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑C                                                                                                                        <  SRR14920191.1894100/151‑1 (MQ=60)

 GCGAAACAGACTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CC                                                                                                                       <  SRR14920191.2779271/151‑1 (MQ=60)

 GCGAAACAGACTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CC                                                                                                                       <  SRR14920191.2994302/151‑1 (MQ=60)

 GCGAAACAGACTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CC                                                                                                                       <  SRR14920191.1510549/151‑1 (MQ=60)

 GCGAAACAGACTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CC                                                                                                                       <  SRR14920191.2290/151‑1 (MQ=60)

 GCGAAACAGACTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CC                                                                                                                       <  SRR14920191.2186390/151‑1 (MQ=60)

         GACTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATT                                                                                                                >  SRR14920191.4746930/1‑151 (MQ=60)

          ACTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTT                                                                                                               >  SRR14920191.311254/1‑151 (MQ=60)

           CTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCCTCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTACAACCAATTGATTTT                                                                                                              >  SRR14920191.418116/1‑151 (MQ=60)

           CTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTT                                                                                                              >  SRR14920191.418156/1‑151 (MQ=60)

           CTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTT                                                                                                              >  SRR14920191.417898/1‑151 (MQ=60)

               GGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATG                                                                                                          <  SRR14920191.2558002/151‑1 (MQ=60)

                            ACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTC                                                                                             <  SRR14920191.2976879/151‑1 (MQ=60)

                                   TTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGCTTTTTATGTCTTTTGAAATTCATCAATC                                                                                      <  SRR14920191.422442/151‑1 (MQ=60)

                                   TTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      <  SRR14920191.3309844/151‑1 (MQ=60)

                                     TTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCag                                                                                    >  SRR14920191.4084498/1‑149 (MQ=60)

                                               ATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgt                                                                          >  SRR14920191.1183304/1‑139 (MQ=60)

                                                      TCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaag                                                                   <  SRR14920191.3897819/151‑20 (MQ=60)

                                                      TCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaag                                                                   <  SRR14920191.1692997/151‑20 (MQ=60)

                                                      TCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaag                                                                   <  SRR14920191.3498339/151‑20 (MQ=60)

                                                      TCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaag                                                                   <  SRR14920191.3743437/151‑20 (MQ=60)

                                                      TCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaag                                                                   <  SRR14920191.2576329/151‑20 (MQ=60)

                                                      TCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaag                                                                   <  SRR14920191.273916/151‑20 (MQ=60)

                                                      TCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaag                                                                   <  SRR14920191.274205/151‑20 (MQ=60)

                                                         CTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtga                                                                >  SRR14920191.3843156/1‑129 (MQ=60)

                                                                TCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatat                                                         <  SRR14920191.443126/151‑30 (MQ=60)

                                                                     TCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttg                                                    <  SRR14920191.2838264/151‑35 (MQ=60)

                                                                     TCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttg                                                    <  SRR14920191.2118479/151‑35 (MQ=60)

                                                                        CTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaa                                                 >  SRR14920191.651797/1‑114 (MQ=60)

                                                                        CTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATGTTTATGTCTTTTGAAATTCATCAATaagattcccttgttaaaaattgatcgatatatttgaaa                                                 >  SRR14920191.4052731/1‑113 (MQ=60)

                                                                          TCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatg                                               >  SRR14920191.2702048/1‑112 (MQ=60)

                                                                             TTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgtgaaaaagtgatcgatatatttgaaatcaag                                            >  SRR14920191.2460399/1‑109 (MQ=60)

                                                                               CCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtt                                          >  SRR14920191.2357516/1‑107 (MQ=60)

                                                                                   ATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgc                                      >  SRR14920191.4356968/1‑103 (MQ=60)

                                                                                        GTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatatt                                 >  SRR14920191.1810384/1‑98 (MQ=60)

                                                                                        GTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatatt                                 >  SRR14920191.37638/1‑98 (MQ=60)

                                                                                            CGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaa                             >  SRR14920191.565044/1‑94 (MQ=60)

                                                                                            CGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaa                             >  SRR14920191.565495/1‑94 (MQ=60)

                                                                                              TCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaatt                           >  SRR14920191.2552854/1‑92 (MQ=60)

                                                                                              TCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaatt                           >  SRR14920191.4227905/1‑92 (MQ=60)

                                                                                              TCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaatt                           >  SRR14920191.3201471/1‑92 (MQ=60)

                                                                                              TCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaatt                           >  SRR14920191.1647129/1‑92 (MQ=60)

                                                                                               CCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattt                          >  SRR14920191.860540/1‑91 (MQ=60)

                                                                                               CCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattt                          >  SRR14920191.1461336/1‑91 (MQ=60)

                                                                                               CCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagctgtctcttatacacatctccgagcccacgagaccgatcag                          >  SRR14920191.2371379/1‑91 (MQ=60)

                                                                                                        AGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaa                 >  SRR14920191.1006037/1‑82 (MQ=60)

                                                                                                        AGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgcatattgaaattttaagccaaa                 >  SRR14920191.1006039/1‑82 (MQ=60)

                                                                                                        AGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgatatatttgaaatcaagtttcgaatattgaaattttaagccaaa                 >  SRR14920191.1303343/1‑82 (MQ=60)

                                                                                                            CCAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCagattgccttgttaaaaagtgatcgctgtatttgaaatcaagtttcgcatattgaaattttaaaccaaaaaag             <  SRR14920191.4551101/151‑74 (MQ=60)

                                                                                                                GTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAAT                                                                                       >  SRR14920191.4878385/1‑73 (MQ=60)

                                                                                                                  GTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTATGAAATTCATCAATC                                                                                      >  SRR14920191.4451801/1‑72 (MQ=60)

                                                                                                                       GTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTGTGAAATTCATCAATCagattgccttgttaaaaagctgtctcttatacacatctccgagcccacgagaccgatcagtatctcgtatgccgtcttctgctt  >  SRR14920191.1862684/1‑67 (MQ=60)

                                                                                                                         AGTGCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      <  SRR14920191.3518729/65‑1 (MQ=60)

                                                                                                                          GTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      >  SRR14920191.4897902/1‑64 (MQ=60)

                                                                                                                              ACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      <  SRR14920191.1298022/60‑1 (MQ=60)

                                                                                                                              ACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      <  SRR14920191.514849/60‑1 (MQ=60)

                                                                                                                              ACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      <  SRR14920191.3819747/60‑1 (MQ=60)

                                                                                                                                       ACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      <  SRR14920191.725235/51‑1 (MQ=60)

                                                                                                                                       ACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      <  SRR14920191.2809828/51‑1 (MQ=60)

                                                                                                                                             AATTTATAACCAATTGCTTTTTATGTCTTTTGAAATTCATCAATC                                                                                      <  SRR14920191.2895306/45‑1 (MQ=60)

                                                                                                                                              ATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      >  SRR14920191.3034068/1‑44 (MQ=60)

                                                                                                                                              ATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      >  SRR14920191.4108993/1‑44 (MQ=60)

                                                                                                                                              ATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      >  SRR14920191.1817444/1‑44 (MQ=60)

                                                                                                                                              ATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      >  SRR14920191.4350960/1‑44 (MQ=60)

                                                                                                                                              ATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      >  SRR14920191.2105830/1‑44 (MQ=60)

                                                                                                                                              AGTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      >  SRR14920191.1932062/1‑44 (MQ=60)

                                                                                                                                               TTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      >  SRR14920191.4421081/1‑43 (MQ=60)

                                                                                                                                               TTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      >  SRR14920191.3880873/1‑43 (MQ=60)

                                                                                                                                               TTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      >  SRR14920191.3880372/1‑43 (MQ=60)

                                                                                                                                                 TATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      <  SRR14920191.732160/41‑1 (MQ=60)

                                                                                                                                                       CAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      <  SRR14920191.3388615/35‑1 (MQ=60)

                                                                                                                                                       CAATTGATTTTTATGTCTTTTGAAATTCATCAATC                                                                                      >  SRR14920191.2147954/1‑35 (MQ=60)

CGCGAAACAGACTCTGGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAA‑CAATTGATTTTTATGTCTTTTGAAATTCATCAATCCTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCCAGGAGGCAGATCATG  >  NZ_CP025268/3743140‑3743408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`