Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F21 I1 R3 778 586.6 19747158 98.0% 19352214 137.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,728,808 (C)10→8 pseudogene (1254‑1255/1457 nt) xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,728,8000C.87.6% 371.0 / 49.6 106pseudogene (1263/1457 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base C (13/0);  new base . (52/40);  total (66/40)
Fisher's exact test for biased strand distribution p-value = 1.55e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NZ_CP0252683,728,8010C.87.9% 324.1 / 62.4 106pseudogene (1262/1457 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base . (47/40);  minor base T (12/0);  total (66/40)
Fisher's exact test for biased strand distribution p-value = 1.36e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC  >  NZ_CP025268/3728661‑3728932
                                                                                                                                           ||                                                                                                                                   
ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                      <  1:541300/140‑1 (MQ=255)
ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                      >  1:6787614/1‑140 (MQ=255)
ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                      <  1:1314788/140‑1 (MQ=255)
ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                      <  2:7462002/140‑1 (MQ=255)
  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                    <  2:415626/140‑1 (MQ=255)
      tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccc                                                                                                                                <  1:4934355/140‑1 (MQ=255)
       aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccccccc                                                                                                                               <  2:7604617/140‑1 (MQ=255)
        aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                              <  2:5965647/140‑2 (MQ=255)
          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                            <  2:5808644/140‑4 (MQ=255)
           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                           <  2:2433451/140‑5 (MQ=255)
           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                           <  2:8822480/140‑5 (MQ=255)
           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                           <  1:8707098/140‑5 (MQ=255)
           ggCACAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                           <  1:7072459/140‑5 (MQ=255)
             cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGgcgcccccgggcgg                                                                                                                         >  2:1449867/1‑134 (MQ=255)
              aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtgcggt                                                                                                                        >  1:3184984/1‑133 (MQ=255)
                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgct                                                                                                             >  2:4911926/1‑140 (MQ=255)
                         cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAgctgc                                                                                                           >  1:9712784/1‑140 (MQ=255)
                         cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGTAATCGAgctgc                                                                                                           >  1:7402033/1‑140 (MQ=255)
                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCTCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCAGa                                                                                                        >  2:5023747/1‑140 (MQ=255)
                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                        <  1:3024642/140‑1 (MQ=255)
                                 aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGACTCGAGCTGCTGACCGCt                                                                                                   >  2:7435754/1‑140 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑GCCCCCCCGTACGGTAATCGCGCTGCTGACCGCTgct                                                                                                >  2:9401857/1‑138 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGTAATCGAGCTGCGGACCggggat                                                                                                >  1:5241182/1‑135 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGACTCGAGCTGCGGACCGCGGag                                                                                                >  1:398923/1‑139 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAAGCGAGCTGCTGGCCGCTGat                                                                                                >  1:942750/1‑140 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGat                                                                                                >  1:3361666/1‑140 (MQ=255)
                                      cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGGGTCGTGCTGCTGACCGCTGgtat                                                                                              >  1:1496411/1‑140 (MQ=255)
                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGTGCTGCTGACCGCTGATATcc                                                                                            >  1:880970/1‑140 (MQ=255)
                                         cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGT‑CGGGAAGCGAGGTGCTGTGCGCTGGTATCCg                                                                                           >  2:5109011/1‑140 (MQ=255)
                                         cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTTCGGGCATCGGGCTGCTGGCCGCTGATATCCg                                                                                           >  1:2151667/1‑140 (MQ=255)
                                              cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGAGGTCCGCCAGc                                                                                      >  1:1800763/1‑140 (MQ=255)
                                              cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTGATCGAGCTGCTGTCCGCTGATATCCGCCAGc                                                                                      >  1:1801138/1‑140 (MQ=255)
                                                aaCGGTAGTTGCGGCAACAATTCAATGTGCGATGTCTCTGGGTGCGCCGCGATCTGCGCCCGCGTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GGCGCCCCCGGACGGTGGTCGGGCTGCGGACCGCTGATCTCCGcgcgcag                                                                                    >  2:717106/1‑134 (MQ=255)
                                                 aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGGAAGCGAGCTGCTGACCGCGGGTAGCCGCCAGCagc                                                                                   >  2:2597922/1‑138 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:4984585/140‑1 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  2:6061190/140‑1 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:4911926/140‑1 (MQ=255)
                                                    gTAGTTGCGGCAACAATTCCATGAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                <  1:8758156/140‑1 (MQ=255)
                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                <  1:6712847/140‑1 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCCGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGAGGGTAATCGGGCGGCGGAGCGCGGTGAGCCGCCCGCATcggtcg                                                                              >  2:9412856/1‑135 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCTC‑‑CTCCCCCCCCGTACGGTCCTCGAGGTGCTGACCGCTGATATCCGCCCGCGTGTGagg                                                                              >  2:3455929/1‑138 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACTT‑‑CGCCCCCCGGGCGGGAATCGAGCTGCGGAGCGCGGAGAGCCGCCAGCGTGTGACg                                                                              >  2:6579543/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACTT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGCCCGCTGATCTCCGCCAGCaggggtgg                                                                              >  1:1887453/1‑133 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CGCGCCCCGTACGGTGCTCGAGCTGCTGGGCGCTGATTTCCGCCGGCATGTGTCg                                                                              >  2:6579913/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTTCGGTAATCGAGGTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              >  1:4465299/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              <  2:941018/140‑1 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACg                                                                              >  1:8360302/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTGCGGGAAGCGAGCTGCTGGCCGCTGATAGCCGCCAGCATCTGCCg                                                                              >  1:7674346/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTACTCGAGCTGCTGTCCGCTGCTATCCGCCAGCATCTGACg                                                                              >  1:7949056/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCCCCCCCGTACGGGAGTCGAGCTGCTGTCCGCTGGTATCCGCCAGCAGCTGACg                                                                              >  1:3240095/1‑140 (MQ=255)
                                                       gTTGCGGCAACATTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                             <  1:3137452/140‑1 (MQ=255)
                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                             <  1:3136728/140‑1 (MQ=255)
                                                           cGGCAACAATTCAATGAGCGATTTCTCTNGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                         <  1:6291639/140‑1 (MQ=255)
                                                           cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCGCCGTGGGGTGATCGTGCTGCTGACGGCTGGTGTCCGCCGGGGTGTGACGCCGGt                                                                         >  2:4612657/1‑140 (MQ=255)
                                                             gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGAGATCCGCCAGCAGCGGACGCCAGTag                                                                       >  2:8991417/1‑139 (MQ=255)
                                                               aacaaTTCAATTAGCGATTTCTCTGGATTAGCAGCTATCTGCGCCATCCTTGCTGCGCCCAGTGCTGGCACCTTAT‑‑ACCCCCCAGTAAGGTAATCTAGATGCTGAACGCTGATATCCGCCATAATCTGACGCCATTACTc                                                                     <  2:8111777/140‑1 (MQ=255)
                                                               aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                                                     <  2:2513477/140‑1 (MQ=255)
                                                                   aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                 <  2:2423663/140‑1 (MQ=255)
                                                                         tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CGCCCCCCGGGCGGTGTGCGAGCTGCTGTCCGCTGGTTTCCGCCAGCAGCTGACGCCAGTACTCACTACGcgcc                                                           >  2:2809994/1‑140 (MQ=255)
                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                          <  1:9658010/140‑1 (MQ=255)
                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                          <  2:3119521/140‑1 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑GCCCCCCCGTACGGTAATCGAGCTGCTGAGCGGTGCTATCAGCCAGCTACTGACGCGCGTGGTCACTACGcgcgccg                                                        >  2:6504774/1‑140 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATGTCCGCCAGCATCTGACGCCAGGACTCACTACGcggcccg                                                        >  1:5216067/1‑140 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGGACTCGAGCTGCTGTCCGCGGGTATCCGCCAGCAGCTGACGCCAGTACTCACTACGcgccccg                                                        >  1:3799479/1‑140 (MQ=255)
                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                      <  1:2166694/140‑1 (MQ=255)
                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                <  1:64229/140‑1 (MQ=255)
                                                                                    gctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGCCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCAGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACAACGCGCCCCGCCCCCCAt                                                <  2:7017174/139‑1 (MQ=255)
                                                                                      tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCa                                              <  1:2322693/140‑1 (MQ=255)
                                                                                             gccgcGAGCTGCGCCAGCCTTGCTGCGCCCAGTGCAGGCCCCAC‑‑CTCCCCCCCCGGACGGGCCTCGACAGGCGCACCGCGGATATCCGCCACCAGCTGACGCGCGTACACACTACGCGCCCCGCCCCCGATCACCGAAac                                       >  2:6843611/1‑140 (MQ=255)
                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAaca                                      <  1:1474077/140‑1 (MQ=255)
                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCCGCAGCTGACGCCAGTACTCACGACGCGCCCCGCCCCCCATCAACGTcaca                                      >  1:4509611/1‑140 (MQ=255)
                                                                                               cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATGTCCGCCCGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCCACGTAacac                                     >  1:7734644/1‑140 (MQ=255)
                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGAGCTGCTGTCCGCTGATGTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTTACTCttt                                  >  1:8175049/1‑140 (MQ=255)
                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTTACACttt                                  >  1:5006608/1‑140 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 <  2:2311895/140‑1 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 <  2:6676700/140‑1 (MQ=255)
                                                                                                           cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                         <  2:1893643/140‑1 (MQ=255)
                                                                                                             gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTAATa                       >  1:4491731/1‑140 (MQ=255)
                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                      >  1:7712668/1‑140 (MQ=255)
                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTGACACTTTGCGGTTTAAttc                      >  1:9512598/1‑138 (MQ=255)
                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCATCGTAACACGTTGCGGGTTTATAc                      >  1:9512871/1‑140 (MQ=255)
                                                                                                                ttGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                    <  1:2597922/140‑1 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                >  2:6946723/1‑78 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa                  <  1:1114987/140‑1 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa                  <  1:1115297/140‑1 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAAGCGAGCTGCTGACCGCTGATGTCCGCCAGCATCTGCCGCCCGGACTCACTCCGCGCCCCGCCCCCACTCACCGTAACACTGTGCGGGGTAATACCGCa                  >  2:6440350/1‑140 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTTCGGGAAGCGCGCTGCTGTCCGCGGATGTCCGCCAGCATCTGACGCCCGTGCTCGCTACGCGGCCCGCGGCCAGGCGACGGAACACTTTGCGGGTTGGTGCCGCa                  >  2:1668021/1‑140 (MQ=255)
                                                                                                                     gcgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc               <  2:6291726/140‑1 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa              <  1:7359808/140‑1 (MQ=255)
                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgc                                                       <  2:4605211/97‑1 (MQ=255)
                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg            >  1:1216212/1‑140 (MQ=255)
                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAAGCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGTCGCCAGTACTCCCTACGCGGCCCGCCCCCCCGCCACGGAGCACGTTGCGGTGTGCTACCGCAGGCATg            >  1:7320833/1‑140 (MQ=255)
                                                                                                                            gTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGCTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAAAACTTTGCGGTTTAATACCGCAGGCATGCACg        <  2:3886121/140‑1 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCCTGCACGa       >  1:9326418/1‑140 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTGACACGTTGCGGGTTTATACCGCAGGCCTGCACGa       >  1:6496373/1‑140 (MQ=255)
                                                                                                                             tGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGGACGCACTACGCGCCCCGCCCCCACGCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGa       >  1:8345736/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTTCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc  >  1:1440441/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTTATTCCGCAGGCATGCACGGCATcc  >  2:2637012/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATcc  >  1:1440711/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACGGCACACTTTGCGGTGTAATACCGCAGGCATGCACGACATcc  >  2:22388/1‑140 (MQ=255)
                                                                                                                                           ||                                                                                                                                   
GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC  >  NZ_CP025268/3728661‑3728932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  NZ_CP025268/3728667‑3728958
                                                                                                                                             |                                                                                                                                                      
TAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAAT                                                                                                                                             <  SRR14920172.4972993/151‑1 (MQ=60)
  AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCG                                                                                                                                           <  SRR14920172.744827/151‑1 (MQ=60)
     GGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGC                                                                                                                                        <  SRR14920172.8776666/151‑1 (MQ=60)
     GGCACAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGC                                                                                                                                        <  SRR14920172.7128573/151‑1 (MQ=60)
        ATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGC                                                                                                                                     >  SRR14920172.9790233/1‑151 (MQ=60)
        ATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGTAATCGAGCTGC                                                                                                                                     >  SRR14920172.7460805/1‑151 (MQ=60)
          AACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTG                                                                                                                                   <  SRR14920172.7994761/151‑1 (MQ=60)
               TGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGC                                                                                                                              <  SRR14920172.2743485/151‑1 (MQ=60)
                   CATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGTAATCGAGCTGCGGACCGgggat                                                                                                                          >  SRR14920172.5282073/1‑146 (MQ=60)
                   CATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGACTCGAGCTGCGGACCGCggag                                                                                                                          >  SRR14920172.402106/1‑147 (MQ=60)
                   CATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAAGCGAGCTGCTGGCCGCTGAT                                                                                                                          >  SRR14920172.950057/1‑151 (MQ=60)
                   CATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑GGCCCCCCCCGGACGGTACTCGAGCTGCTGTCCGCTGTT                                                                                                                          >  SRR14920172.1638032/1‑151 (MQ=60)
                   CATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGAT                                                                                                                          >  SRR14920172.3388183/1‑151 (MQ=60)
                     TCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGGGTCGTGCTGCTGACCGCTGGTAT                                                                                                                        >  SRR14920172.1508175/1‑151 (MQ=60)
                      CTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATC                                                                                                                       <  SRR14920172.3048316/151‑1 (MQ=60)
                       TGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGTGCTGCTGACCGCTGATATCC                                                                                                                      >  SRR14920172.887760/1‑151 (MQ=60)
                        GGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTTCGGGCATCGGGCTGCTGGCCGCTGATATCCG                                                                                                                     >  SRR14920172.2168476/1‑151 (MQ=60)
                             ATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGAGCTGCTGCCCGCGGATAGCCGCCAGC                                                                                                                >  SRR14920172.8091256/1‑151 (MQ=60)
                             ATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGAGGTCCGCCAGC                                                                                                                >  SRR14920172.1814717/1‑151 (MQ=60)
                             ATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTGATCGAGCTGCTGTCCGCTGATATCCGCCAGC                                                                                                                >  SRR14920172.1815092/1‑151 (MQ=60)
                                     GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACTT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGCCCGCTGATCTCCGCCAGCAggggtgg                                                                                                        >  SRR14920172.1902084/1‑144 (MQ=60)
                                     GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTTCGGTAATCGAGGTGCTGACCGCTGATATCCGCCAGCATCTGACG                                                                                                        >  SRR14920172.4500374/1‑151 (MQ=60)
                                     GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACG                                                                                                        >  SRR14920172.8427006/1‑151 (MQ=60)
                                     GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCNGACCGCTGATATCCGCCAGCATCTGACG                                                                                                        >  SRR14920172.6341391/1‑151 (MQ=60)
                                     GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTGCGGGAAGCGAGCTGCTGGCCGCTGATAGCCGCCAGCATCTGCCG                                                                                                        >  SRR14920172.7735426/1‑151 (MQ=60)
                                     GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTACTCGAGCTGCTGTCCGCTGCTATCCGCCAGCATCTGACG                                                                                                        >  SRR14920172.8012265/1‑151 (MQ=60)
                                     GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCCCCCCCGTACGGGAGTCGAGCTGCTGTCCGCTGGTATCCGCCAGCAGCTGACG                                                                                                        >  SRR14920172.3265585/1‑151 (MQ=60)
                                          AACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTCATCGAGCTGCTGACCGCGGATATCCGCCAGCAGCTGACGGCAGT                                                                                                   >  SRR14920172.1329007/1‑151 (MQ=60)
                                            CGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAC                                                                                                 <  SRR14920172.5023572/151‑1 (MQ=60)
                                            CGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCATTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAC                                                                                                 <  SRR14920172.4950407/151‑1 (MQ=60)
                                              atacgagattactacgcgtctcgtgggctcggagatgtgtataagagacagGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTC                                                                                               <  SRR14920172.7001847/100‑1 (MQ=60)
                                              GTAGTTGCGGCAACAATTCCATGAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTC                                                                                               <  SRR14920172.8828117/151‑1 (MQ=60)
                                              GTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTC                                                                                               <  SRR14920172.6766052/151‑1 (MQ=60)
                                                 GTTGCGGCAACATTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACT                                                                                            <  SRR14920172.3162078/151‑1 (MQ=60)
                                                 GTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACT                                                                                            <  SRR14920172.3161350/151‑1 (MQ=60)
                                                  ccccccccccccttttcaagcagaagacggcatacgagattactacgcgtctcgtgggctcggagatgtgtataagagacagcatCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAC                                                                                             <  SRR14920172.96942/66‑1 (MQ=60)
                                                  TTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTA                                                                                           <  SRR14920172.8688649/151‑1 (MQ=60)
                                                     CGGCAACAATTCAATGAGCGATTTCTCTNGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGC                                                                                        <  SRR14920172.6341304/151‑1 (MQ=60)
                                                           CAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATGTCCGCCAGCATCTGACGCCAGGACTCACTACGCGGCCCG                                                                                  >  SRR14920172.5256765/1‑151 (MQ=60)
                                                           CAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGGACTCGAGCTGCTGTCCGCGGGTATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCG                                                                                  >  SRR14920172.3829441/1‑151 (MQ=60)
                                                                    GAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATC                                                                         <  SRR14920172.9735026/151‑1 (MQ=60)
                                                                        GATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACG                                                                     <  SRR14920172.2183496/151‑1 (MQ=60)
                                                                        GATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACG                                                                     <  SRR14920172.2183638/151‑1 (MQ=60)
                                                                             CTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCCGCAGCTGACGCCAGTACTCACGACGCGCCCCGCCCCCCATCAACGTCACA                                                                >  SRR14920172.4545013/1‑151 (MQ=60)
                                                                              TCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATGTCCGCCCGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCCACGTAACAC                                                               >  SRR14920172.7796209/1‑151 (MQ=60)
                                                                              TCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACAC                                                               <  SRR14920172.64820/151‑1 (MQ=60)
                                                                               CTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACT                                                              <  SRR14920172.4149686/151‑1 (MQ=60)
                                                                                TGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTT                                                             <  SRR14920172.2340941/151‑1 (MQ=60)
                                                                                 GGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGAGCTGCTGTCCGCTGATGTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTTACTCTTT                                                            >  SRR14920172.8240205/1‑151 (MQ=60)
                                                                                 GGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTTACACTTT                                                            >  SRR14920172.5045765/1‑151 (MQ=60)
                                                                                        CCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCGACGGAACACTTTGCGGTTT                                                     >  SRR14920172.9658370/1‑151 (MQ=60)
                                                                                        CCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTT                                                     <  SRR14920172.1485667/151‑1 (MQ=60)
                                                                                            GATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTAATA                                                 >  SRR14920172.4526995/1‑151 (MQ=60)
                                                                                             ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑‑CCCCCCCCGGACGGTGATCGGGCTGCTGACCGCGGCTATCCGCCCGCATCTGGCGCCGGTACTCGCTGCGCGCCCCGCCCCCctgccacgggacacgttgcggggtttgag                                                >  SRR14920172.5087784/1‑122 (MQ=60)
                                                                                             ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAC                                                >  SRR14920172.7774040/1‑151 (MQ=60)
                                                                                             ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTGACACTTTGCGGTTTAATTC                                                >  SRR14920172.9588418/1‑151 (MQ=60)
                                                                                             ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCATCGTAACACGTTGCGGGTTTATAC                                                >  SRR14920172.9588693/1‑151 (MQ=60)
                                                                                                 GCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTtcgggtgtagaccgcg                                            >  SRR14920172.9066921/1‑135 (MQ=60)
                                                                                                     CAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCA                                        <  SRR14920172.5253155/151‑1 (MQ=60)
                                                                                                       GCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGTGTTCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTTATACCGCAGGCATG                                      >  SRR14920172.9344113/1‑151 (MQ=60)
                                                                                                       GCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGGCGCCAGTACGCACTACGCGCCCCGCCCCCAATCAACGTTCCACTTTGCGGTTTAATACCGCAGGCCTG                                      >  SRR14920172.6538140/1‑151 (MQ=60)
                                                                                                       GCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATG                                      >  SRR14920172.1225870/1‑151 (MQ=60)
                                                                                                       GCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACctgtctcgtatacacatctccgagcccacgag                                      >  SRR14920172.4641378/1‑119 (MQ=60)
                                                                                                       GCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAAGCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGTCGCCAGTACTCCCTACGCGGCCCGCCCCCccgccacggagcacgttgcggtgtgctaccgcaggcatg                                      >  SRR14920172.7378939/1‑112 (MQ=60)
                                                                                                       GCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCGGATGTCCGCCCGCTTCTGACGCCCGTACTCACTACGCGCCCCGCCCCCACTCAACGGGACACGTTGCGGGGGAATTCCGCAGGCATG                                      >  SRR14920172.5147667/1‑151 (MQ=60)
                                                                                                          TTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAC                                   <  SRR14920172.2618324/151‑1 (MQ=60)
                                                                                                            GCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGA                                 <  SRR14920172.1123794/151‑1 (MQ=60)
                                                                                                            GCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGA                                 <  SRR14920172.1124106/151‑1 (MQ=60)
                                                                                                            GCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCCTGCACGA                                 >  SRR14920172.9400778/1‑151 (MQ=60)
                                                                                                            GCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTGACACGTTGCGGGTTTATACCGCAGGCCTGCACGA                                 >  SRR14920172.6547691/1‑151 (MQ=60)
                                                                                                            GCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGGACGCACTACGCGCCCCGCCCCCACGCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGA                                 >  SRR14920172.8412339/1‑151 (MQ=60)
                                                                                                                CGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATC                             <  SRR14920172.7418219/151‑1 (MQ=60)
                                                                                                                 GCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTTCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC                            >  SRR14920172.1451767/1‑151 (MQ=60)
                                                                                                                 GCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATCC                            >  SRR14920172.1452039/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCCGGCCTGCACGACAGCCATGCC                       >  SRR14920172.1026967/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACctgtctcgtatacacatctccgagcccacgagacgcgtag                       >  SRR14920172.2867003/1‑111 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATCCCGCAGGCATGCACGACATCCATGCC                       >  SRR14920172.8505082/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACCTCCATGCC                       >  SRR14920172.2209503/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920172.4621879/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920172.5693936/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920172.7740847/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920172.7740766/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTGAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920172.2658358/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTATGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920172.174435/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACATTGCGGGTTAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920172.2777590/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCCGTACTCACTCCGCGCCCCGCCCCCAATCACCGTAACACTGTGCGGTTTAATACCGCAGGCATGCCCGACAGCCATGCC                       >  SRR14920172.8248230/1‑151 (MQ=60)
                                                                                                                      GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAAGCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920172.393680/1‑151 (MQ=60)
                                                                                                                            GCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGC                 >  SRR14920172.6159446/1‑151 (MQ=60)
                                                                                                                             CCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCC                >  SRR14920172.3911697/1‑151 (MQ=60)
                                                                                                                             CCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCC                >  SRR14920172.7553072/1‑151 (MQ=60)
                                                                                                                              CCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCA               >  SRR14920172.5294947/1‑151 (MQ=60)
                                                                                                                              CCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCA               >  SRR14920172.3349711/1‑151 (MQ=60)
                                                                                                                              CCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCA               >  SRR14920172.2053345/1‑151 (MQ=60)
                                                                                                                              CCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCA               >  SRR14920172.3349726/1‑151 (MQ=60)
                                                                                                                              CCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCA               >  SRR14920172.1820927/1‑151 (MQ=60)
                                                                                                                                CACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAAGCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGC             >  SRR14920172.9512135/1‑151 (MQ=60)
                                                                                                                                  cacatCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGC             >  SRR14920172.2312969/6‑151 (MQ=60)
                                                                                                                                   acatCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCG            >  SRR14920172.8639625/5‑151 (MQ=60)
                                                                                                                                    catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTCCTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920172.4544186/4‑151 (MQ=60)
                                                                                                                                    catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCACCGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           <  SRR14920172.8129004/148‑1 (MQ=60)
                                                                                                                                    catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920172.4220104/4‑151 (MQ=60)
                                                                                                                                    catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920172.3888699/4‑151 (MQ=60)
                                                                                                                                    catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920172.4152171/4‑151 (MQ=60)
                                                                                                                                    catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920172.2997395/4‑151 (MQ=60)
                                                                                                                                    catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920172.5568999/4‑151 (MQ=60)
                                                                                                                                    catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920172.6695768/4‑151 (MQ=60)
                                                                                                                                     atCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCA          >  SRR14920172.12402/3‑151 (MQ=60)
                                                                                                                                     atCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAAGCCATCTGCCAGCGCA          >  SRR14920172.2550311/3‑151 (MQ=60)
                                                                                                                                        CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAA       >  SRR14920172.58385/1‑151 (MQ=60)
                                                                                                                                        CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAA       >  SRR14920172.1404895/1‑151 (MQ=60)
                                                                                                                                        CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAA       >  SRR14920172.6663691/1‑151 (MQ=60)
                                                                                                                                         CCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCATCATCTGACGCCAGTACTAACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAC      >  SRR14920172.2783878/1‑151 (MQ=60)
                                                                                                                                         CCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAC      <  SRR14920172.8612822/151‑1 (MQ=60)
                                                                                                                                          CCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACC     >  SRR14920172.2143563/1‑151 (MQ=60)
                                                                                                                                          CCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACC     >  SRR14920172.2143670/1‑151 (MQ=60)
                                                                                                                                           CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCC    <  SRR14920172.7494809/151‑1 (MQ=60)
                                                                                                                                           CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCC    <  SRR14920172.8068716/151‑1 (MQ=60)
                                                                                                                                             CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  SRR14920172.6579381/1‑151 (MQ=60)
                                                                                                                                             CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  SRR14920172.264403/1‑151 (MQ=60)
                                                                                                                                             CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  SRR14920172.5588709/1‑151 (MQ=60)
                                                                                                                                             CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  SRR14920172.7934562/1‑151 (MQ=60)
                                                                                                                                             |                                                                                                                                                      
TAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  NZ_CP025268/3728667‑3728958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑