Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I1 R2	870	341.9	11209456	97.2%	10895591	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,728,808	(C)_10→8	pseudogene (1254‑1255/1457 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,728,800	0	C	.	96.7%	416.7 / 4.5	92	pseudogene (1263/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (2/1); new base . (39/50); total (41/51)
Fisher's exact test for biased strand distribution p-value = 5.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	3,728,801	0	C	.	93.4%	379.1 / 19.8	92	pseudogene (1262/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (4/2); new base . (36/49); total (41/51)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933

||

ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                       <  2:390921/140‑1 (MQ=255)

ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                       <  2:1119836/140‑1 (MQ=255)

   tcaTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACTATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccc                                                                                                                                    <  2:4674101/138‑1 (MQ=255)

     aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccccc                                                                                                                                  >  1:4801085/1‑140 (MQ=255)

      tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccc                                                                                                                                 <  2:3689919/140‑1 (MQ=255)

        aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               <  1:3277407/140‑2 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtt                                                                                                                             >  1:5037561/1‑137 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                             <  2:696747/140‑4 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                             <  2:3697067/140‑4 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                             <  2:3697020/140‑4 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                             <  1:3682879/140‑4 (MQ=255)

           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                            <  1:4108643/140‑5 (MQ=255)

             cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGGCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtacgg                                                                                                                          >  2:3697653/1‑134 (MQ=255)

                  cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATc                                                                                                                   >  1:5099121/1‑140 (MQ=255)

                  cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATc                                                                                                                   >  1:5099183/1‑140 (MQ=255)

                    cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGa                                                                                                                 >  1:5038771/1‑140 (MQ=255)

                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGCACGGTAATCGAg                                                                                                                >  2:2346731/1‑140 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGGAATCGAgct                                                                                                              >  1:202493/1‑140 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgct                                                                                                              >  1:203187/1‑140 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgct                                                                                                              >  1:5581607/1‑140 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCCAgct                                                                                                              >  2:4175699/1‑140 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAACCGAgct                                                                                                              >  1:5123864/1‑140 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAgct                                                                                                              >  1:4339719/1‑140 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATCCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgct                                                                                                              >  2:4860937/1‑140 (MQ=255)

                         cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgc                                                                                                            <  2:2630191/140‑1 (MQ=255)

                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                           <  2:5425234/140‑1 (MQ=255)

                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                           <  2:5535965/140‑1 (MQ=255)

                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                         >  2:33453/1‑140 (MQ=255)

                                tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                     <  1:2438184/140‑1 (MQ=255)

                                  gATGCGACTGTTCTAACGGTAGTTGCGGCACCAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCCGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGAGCTGCTGCCCGCTg                                                                                                   <  1:1638171/140‑1 (MQ=255)

                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGa                                                                                                  >  1:4570135/1‑140 (MQ=255)

                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCCGat                                                                                                 >  1:3810842/1‑140 (MQ=255)

                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCC‑‑TACGTGAAGGGAGCTGCTTACCGCTGATATcc                                                                                             >  2:4714524/1‑140 (MQ=255)

                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTGATAGAGCTGCTGACCGCTGTTATcc                                                                                             >  2:425674/1‑140 (MQ=255)

                                            ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                         >  1:2092529/1‑140 (MQ=255)

                                             tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                        <  1:2400866/140‑1 (MQ=255)

                                                 aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                    >  1:3060325/1‑140 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGCGCGCTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCt                                                                                   <  2:3541221/140‑1 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:3541204/140‑1 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:3683372/140‑1 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:5271579/140‑1 (MQ=255)

                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  1:5290977/140‑1 (MQ=255)

                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  1:1161085/140‑1 (MQ=255)

                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  1:1686745/140‑1 (MQ=255)

                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCGTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  1:2899064/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                               >  1:3325804/1‑124 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               <  2:4562459/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               <  2:3631679/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               >  2:1724154/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCCCTGATATCCGCCAGCATCTGACg                                                                               >  1:1263175/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGGGCTGGCCCGACAT‑‑GCCCCCCCGGACGGGAATCGAACTTCTGGCCGCTGGTGTGCGGCGGCCTCTGACg                                                                               >  2:4808818/1‑140 (MQ=255)

                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  1:3641081/140‑1 (MQ=255)

                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:1695870/140‑1 (MQ=255)

                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCGAGTGCTGGCCCAACAT‑‑CCCCCCCCGTACGGTAATCCAGCTGCTGACCCCGGAGACCCGCCAGGCGCTGACGcc                                                                             >  2:2253903/1‑140 (MQ=255)

                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                             <  1:4860937/140‑1 (MQ=255)

                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                             <  2:1346426/140‑1 (MQ=255)

                                                          gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                           <  2:4517795/140‑1 (MQ=255)

                                                           cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                          >  2:3400018/1‑140 (MQ=255)

                                                             gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCC‑‑TACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                                                                        >  2:4763125/1‑140 (MQ=255)

                                                             gCAACAATTCAATGAGCGATTCCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                                                                        <  2:3169380/140‑1 (MQ=255)

                                                               aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:1767903/132‑1 (MQ=255)

                                                               aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              >  1:1767903/1‑132 (MQ=255)

                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CTCCCCCCGTACTGGTATCGAGCTGCTGACCGCTGATATCCGCCAGGATCTGACTGTAGTACTCACTACGcgctccg                                                         >  2:5085283/1‑140 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGCGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:260997/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:667566/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:1724154/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:4155994/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:3559409/140‑1 (MQ=255)

                                                                                            cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa                                         <  1:5018279/140‑1 (MQ=255)

                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac                                        >  1:3302623/1‑140 (MQ=255)

                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑GCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGGATGTCACGCCAGCACTCACTACGCGCCGCGCCCCCACTCAACGTAaca                                       >  2:4613779/1‑140 (MQ=255)

                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:3151193/140‑1 (MQ=255)

                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:4714524/140‑1 (MQ=255)

                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  2:2330749/140‑1 (MQ=255)

                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:1268595/140‑1 (MQ=255)

                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:297949/140‑1 (MQ=255)

                                                                                                    tCTGCGCCCGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  2:2278688/140‑1 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGGGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCTCCCGCAATCAACGTAACACTTTGCGGTTTAATAc                       >  1:2663555/1‑140 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCAGCTGACGCCATTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATAc                       >  1:3999830/1‑140 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAACTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGGCCCGCCCCCAATCAACGCAACACTGTGCGGTTTAAGAc                       >  2:4921008/1‑140 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACA‑‑CCCCCCCCCGTACGGTAAGCGAGGTGCTGACCGCTGATATTCGCCAGCAGCTGACGCCAGTACTCACTACGGGCCCCGCCCCCCATCAACGTAACACTTTTCGGTTTAATAc                       >  1:3377221/1‑140 (MQ=255)

                                                                                                                ttGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                     <  2:3543987/140‑1 (MQ=255)

                                                                                                                ttGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                     <  1:2813052/140‑1 (MQ=255)

                                                                                                                ttGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                     <  1:1516269/140‑1 (MQ=255)

                                                                                                                ttGCTGCGACCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAATACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                     <  2:2145612/140‑1 (MQ=255)

                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑ACCCCCCCCG‑ACGGTAAACGAGCTGGTGACCGCTGATATCCGCCAGCATATGACGCCAGTACTCACTACGCGCCCCGCCCCCCAACAACGTAACACTTTGTGGTTTAATACCGCa                   >  1:2166908/1‑140 (MQ=255)

                                                                                                                   ctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                  <  1:3697653/140‑1 (MQ=255)

                                                                                                                      cgcCCGGTGCGGGCCCCAC‑‑CCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  1:1675890/140‑1 (MQ=255)

                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  1:1419321/140‑1 (MQ=255)

                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  1:1419588/140‑1 (MQ=255)

                                                                                                                       gcCCAGTGCTGGCCCCTCTT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTGTAATACAGCAGGAAt              <  2:3296272/140‑1 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg             >  1:2276584/1‑140 (MQ=255)

                                                                                                                            gTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg         <  1:1208369/140‑1 (MQ=255)

                                                                                                                            gTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg         <  2:2508850/140‑1 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa        <  2:3634750/140‑1 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGCCATGCCCGa        >  1:3764210/1‑140 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACCCGCCACGCCCCCAATCAACGCAACACTGTCCGGTTTAATACCGCAGGCATGCACGa        >  2:2327053/1‑140 (MQ=255)

                                                                                                                              gCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGAc       <  1:68678/140‑1 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCTGGCGCCGACTCCCCCCCCCTCCCTCAATCAACGCGACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc   >  2:3476929/1‑140 (MQ=255)

                                                                                                                                   ccccACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa  <  2:2537584/140‑1 (MQ=255)

||

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA  >  NZ_CP025268/3728664‑3728957

TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCTATCTGCGCCAGCCTTGCTGCGCCGAGTCCTGGCCCCACAT‑CCCCCCCCCGTACGG                                                                                                                                                <  SRR14920167.3826548/151‑1 (MQ=60)

    AAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATC                                                                                                                                           >  SRR14920167.5110850/1‑151 (MQ=60)

    AAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATC                                                                                                                                           >  SRR14920167.5110788/1‑151 (MQ=60)

     AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCG                                                                                                                                          <  SRR14920167.3284503/151‑1 (MQ=60)

      GCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGA                                                                                                                                         >  SRR14920167.5050315/1‑151 (MQ=60)

       CGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAG                                                                                                                                        <  SRR14920167.3690861/151‑1 (MQ=60)

        GGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGC                                                                                                                                       <  SRR14920167.4117830/151‑1 (MQ=60)

         GCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGGAATCGAGCT                                                                                                                                      >  SRR14920167.203006/1‑151 (MQ=60)

         GCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCT                                                                                                                                      >  SRR14920167.203702/1‑151 (MQ=60)

         GCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCT                                                                                                                                      >  SRR14920167.5594006/1‑151 (MQ=60)

         GCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAACCGAGCT                                                                                                                                      >  SRR14920167.5135580/1‑151 (MQ=60)

         GCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCT                                                                                                                                      >  SRR14920167.4349403/1‑151 (MQ=60)

                      CATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGA                                                                                                                          >  SRR14920167.4580409/1‑151 (MQ=60)

                      CATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCCGAT                                                                                                                         >  SRR14920167.3819027/1‑151 (MQ=60)

                             TAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCC                                                                                                                  <  SRR14920167.2443242/151‑1 (MQ=60)

                              AGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCA                                                                                                                 >  SRR14920167.2096916/1‑151 (MQ=60)

                               GATGCGACTGTTCTAACGGTAGTTGCGGCACCAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCCGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGAGCTGCTGCCCGCTGATATCCGCCAG                                                                                                                <  SRR14920167.1641518/151‑1 (MQ=60)

                                   CGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATC                                                                                                            >  SRR14920167.3066661/1‑151 (MQ=60)

                                        GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCCCTGATATCCGCCAGCATCTGACG                                                                                                       >  SRR14920167.1265747/1‑151 (MQ=60)

                                        GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCGGCTGACCGCTGATATCCGCCAGCATCctgtc                                                                                                       >  SRR14920167.3333044/1‑146 (MQ=60)

                                          TCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCC                                                                                                     <  SRR14920167.2405852/151‑1 (MQ=60)

                                                 GTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTC                                                                                              <  SRR14920167.1163441/151‑1 (MQ=60)

                                                 GTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTC                                                                                              <  SRR14920167.1690205/151‑1 (MQ=60)

                                                 GTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTC                                                                                              >  SRR14920167.1771539/1‑151 (MQ=60)

                                                 GTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTC                                                                                              <  SRR14920167.5302954/151‑1 (MQ=60)

                                                 GTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCGTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTC                                                                                              <  SRR14920167.2905023/151‑1 (MQ=60)

                                                    GTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACT                                                                                           <  SRR14920167.3648997/151‑1 (MQ=60)

                                                     TTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTA                                                                                          <  SRR14920167.4872011/151‑1 (MQ=60)

                                                                               TCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAAC                                                                >  SRR14920167.3309757/1‑151 (MQ=60)

                                                                                 TCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACAC                                                              <  SRR14920167.1727697/151‑1 (MQ=60)

                                                                                         CGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGT                                                      <  SRR14920167.5029780/151‑1 (MQ=60)

                                                                                                ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGGGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCTCCCGCAATCAACGTAACACTTTGCGGTTTAATAC                                               >  SRR14920167.2669059/1‑151 (MQ=60)

                                                                                                ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCAGCTGACGCCATTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATAC                                               >  SRR14920167.4008528/1‑151 (MQ=60)

                                                                                                ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAC                                               <  SRR14920167.3158014/151‑1 (MQ=60)

                                                                                                ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAC                                               <  SRR14920167.1271177/151‑1 (MQ=60)

                                                                                                ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAC                                               <  SRR14920167.4725215/151‑1 (MQ=60)

                                                                                                ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAC                                               <  SRR14920167.298676/151‑1 (MQ=60)

                                                                                                ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑CCCCCCCCCGTACGGTAAGCGAGGTGCTGACCGCTGATATTCGCCAGCAGCTGACGCCAGTACTCACTACGGGCCCCGCCCCCCATCAACGTAACACTTTTCGGTTTAATAC                                               >  SRR14920167.3384736/1‑151 (MQ=60)

                                                                                                    GCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATACCCCCCCC‑‑GACGGTAAACGAGCTGGTGACCGCTGATATCCGCCAGCATATGACGCCAGTACTCACTACGCGCCCCGCCCCCCAACAACGTAACACTTTGTGGTTTAATACCGCA                                           >  SRR14920167.2171504/1‑151 (MQ=60)

                                                                                                          GCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGCGCTGCGGACCGCTGAGATCCGCCAGCCTCTGACGCCAGGACTCACTACGCGCGCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATG                                     >  SRR14920167.2281639/1‑151 (MQ=60)

                                                                                                          GCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATG                                     >  SRR14920167.2281374/1‑151 (MQ=60)

                                                                                                             TTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAC                                  <  SRR14920167.1519353/151‑1 (MQ=60)

                                                                                                             TTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAC                                  <  SRR14920167.2818855/151‑1 (MQ=60)

                                                                                                              TGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTTCTGACCGGCGGTATCCGCCAGCATCTGCCGCCAGTACTCACGACGCCCCCCCCCCCCCATCAATCTAAAACTTTGCGTGTTAATACCGCAGGCATGCACG                                 >  SRR14920167.2715543/1‑151 (MQ=60)

                                                                                                               GCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGCCATGCCCGA                                >  SRR14920167.3772333/1‑151 (MQ=60)

                                                                                                                CTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGAC                               <  SRR14920167.3705658/151‑1 (MQ=60)

                                                                                                                   CGCCCGGTGCGGGCCCCAC‑‑CCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATC                            <  SRR14920167.1679318/151‑1 (MQ=60)

                                                                                                                   CGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATC                            <  SRR14920167.1422433/151‑1 (MQ=60)

                                                                                                                   CGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATC                            <  SRR14920167.1422165/151‑1 (MQ=60)

                                                                                                                    GCCCAGTGCTGGCTCCACAT‑‑CCCCCCCCGGACGGGAATCGAGCGGCTGACCGCTGTTATTCGCCAGCATTTTACGCCCGTACTCACTACGCGCCCCGCCCCCAATCAACGgcacgcttttcggggtaagaccacacgcacgccccacaacc                           >  SRR14920167.525372/1‑110 (MQ=60)

                                                                                                                         GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGCTACCCGCCAGCATCTGACGCCAGTACTCACTACCCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATCCCGCAGGCATGCACCACATCCATGCC                      >  SRR14920167.5590281/1‑151 (MQ=60)

                                                                                                                         GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATTCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                      >  SRR14920167.4073068/1‑151 (MQ=60)

                                                                                                                         GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                      >  SRR14920167.2291055/1‑151 (MQ=60)

                                                                                                                         GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTCCGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                      >  SRR14920167.1266561/1‑151 (MQ=60)

                                                                                                                         GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATTCC                      >  SRR14920167.422443/1‑151 (MQ=60)

                                                                                                                         GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                      <  SRR14920167.1210830/151‑1 (MQ=60)

                                                                                                                         GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATCCATGCC                      >  SRR14920167.1697625/1‑151 (MQ=60)

                                                                                                                         GTGCTGGCCCCACAT‑‑CCCCCCCCATACGGTAATCGAGGTGCTGACCGCTGAAAACCGCCAAGAACTGACGCCAATACTCACCAAGCACCCCGCCCCCAATCAACGTAAAACTTTGCTGTTTAATACCGCAGGCAAGCCAGACATCCATTCC                      >  SRR14920167.3862157/1‑151 (MQ=54)

                                                                                                                           GCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCAT                    <  SRR14920167.68889/151‑1 (MQ=60)

                                                                                                                                CCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTACCCGCCCCGCCCCCCATCAACGTAACACTTTGCGGCTTAATACCCCAGGCATGCACGACATCCATGCCATCTGCC               >  SRR14920167.5244367/1‑151 (MQ=60)

                                                                                                                                CCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCC               <  SRR14920167.968807/151‑1 (MQ=60)

                                                                                                                                CCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCC               >  SRR14920167.1465236/1‑151 (MQ=60)

                                                                                                                                    ACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCG           <  SRR14920167.527560/151‑1 (MQ=60)

                                                                                                                                      acatCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCG           >  SRR14920167.1514030/5‑151 (MQ=60)

                                                                                                                                       catCCCCCCCCGTACGGTAATCGAGCTGCTGAACGCTGATATCCGCCAGCATCTGACGCCAGTACGCACTACGCGCCCCGCCCCCACTCAACGTAACACCTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTCCCAGCGC          >  SRR14920167.2412910/4‑151 (MQ=60)

                                                                                                                                         TCCCCCCCCGTACGGTAATCGAGCTGCCGACCGCTGATATCCCCCAGCATCTTACGCCCGTACTCACCACCCGCCCCGCCCCCGATCAACGTAACACTTTTCGGTTTAATACCCCAGGCCTGCCCCACATCCCTGCCCTCTGCCATCGCAG        >  SRR14920167.2513460/1‑151 (MQ=60)

                                                                                                                                             CCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACC    >  SRR14920167.2619624/1‑151 (MQ=60)

                                                                                                                                              CCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCC   >  SRR14920167.2211510/1‑151 (MQ=60)

                                                                                                                                               CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA  >  SRR14920167.1946344/1‑151 (MQ=60)

TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA  >  NZ_CP025268/3728664‑3728957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`