Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F21 I1 R6 776 393.9 12922803 98.2% 12690192 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,728,808 (C)10→8 pseudogene (1254‑1255/1457 nt) xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,728,8000C.89.2% 345.4 / 35.0 93pseudogene (1263/1457 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base C (7/3);  new base . (48/35);  total (55/38)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NZ_CP0252683,728,8010C.89.5% 293.6 / 49.2 93pseudogene (1262/1457 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base . (42/35);  minor base G (9/0);  total (55/38)
Fisher's exact test for biased strand distribution p-value = 9.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933
                                                                                                                                           ||                                                                                                                                    
ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                       <  1:2060953/140‑1 (MQ=255)
  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccg                                                                                                                                     >  1:283119/1‑139 (MQ=255)
      tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccc                                                                                                                                 <  2:2468919/140‑1 (MQ=255)
        aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               <  1:66278/140‑2 (MQ=255)
           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                            <  1:2042413/140‑5 (MQ=255)
           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                            <  2:3478954/140‑5 (MQ=255)
            gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccgccgccgggcg                                                                                                                           >  2:594662/1‑135 (MQ=255)
              aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtacggt                                                                                                                         <  1:601842/140‑8 (MQ=255)
                  cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATc                                                                                                                   >  1:2683367/1‑140 (MQ=255)
                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                                <  2:2726798/140‑1 (MQ=255)
                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑T‑GGCCCCCCCGTACGGtctgggtg                                                                                                                >  1:3719690/1‑133 (MQ=255)
                     tGCGCATCTGGAAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGAACGGGAATCGAg                                                                                                                >  1:845925/1‑140 (MQ=255)
                        gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                             <  1:2153321/140‑1 (MQ=255)
                        gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                             <  1:2154597/140‑1 (MQ=255)
                         cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTGCGGTACTCGAgctgc                                                                                                            >  2:4179919/1‑140 (MQ=255)
                         cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCACCAT‑‑CCCCCCCCGGCCGGTCATCGAgctgc                                                                                                            >  2:4179970/1‑140 (MQ=255)
                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                         <  2:6189442/140‑1 (MQ=255)
                                tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                     <  2:4996827/140‑1 (MQ=255)
                                tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCACCCGTACGGTAATCGAGCTTCTGACCGc                                                                                                     <  2:4058691/140‑1 (MQ=255)
                                   aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑‑CCGCCCCCGGCCGGTACTCGAGCTGCGGAgcgcggt                                                                                                  >  1:5298436/1‑137 (MQ=255)
                                     gCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                <  1:5265872/140‑1 (MQ=255)
                                       gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGAtctc                                                                                              >  2:6178623/1‑140 (MQ=255)
                                       gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCGCCCCCGGACGGGCAGCGGGCTGCTGACCGCGGAGATc                                                                                              >  2:6178832/1‑140 (MQ=255)
                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTGCGGTTATCGAGCTGGTGACCGCGGATGTcc                                                                                             >  2:598115/1‑140 (MQ=255)
                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTGCGGTGGGCGTGCTGCTGGCCGCTggggtgc                                                                                             >  1:5010870/1‑134 (MQ=255)
                                         cTGGTCTAACGGTAGTTGCGGCACCACTTCAATGAGCGATTGCTCTGGCTGCGCCGCGAGCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCA‑‑GGCGCCCCCGTACGGGAGGCGAGCGGCGGACCGCGGAGGTCCg                                                                                            >  2:2744405/1‑140 (MQ=255)
                                                 aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCGGCGCCCGGACGGGTATGGGGCTGCTGGCCGCTGATTGCCGCCAGCCTc                                                                                    >  1:166655/1‑140 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:2060475/140‑1 (MQ=255)
                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑‑CCCCCCCCGGACGGTGAGCGCGCTGCTGGCCGCTGGTGTCCGCCGGCAGCTgg                                                                                 >  1:4428449/1‑139 (MQ=255)
                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  2:2147524/140‑1 (MQ=255)
                                                      aGTTGCGGCAACAATTCATTGAGCGATTTCTCTGTATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCACCCCCGTCCGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               >  2:1723679/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CGCCCCCCGGAGGGTAATCGAGCTGCTGACCGCTGATTTCCGCCAGGTTCTGACg                                                                               >  1:4196454/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCGGATATCCGCCCGCCGCTGACg                                                                               >  1:2797293/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               <  1:6088016/140‑1 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAAGCGGGCTGCGGACCGCGGATATCCGCCAGCATCTGACg                                                                               >  2:2530639/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACg                                                                               >  1:2796862/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGGCATCGAGCTGGGGACCGCTGATGTCCGGCAGCATCTGACg                                                                               >  1:6069459/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCACCAGCCTTGCTGCGCCCAGTGCTGGCCCATCAT‑‑CCCCCCCCGTTCGGGATTCGAGCTGCTGACCGATGATATCAGCAAGCATCTGACg                                                                               >  2:4334170/1‑140 (MQ=255)
                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:4259421/140‑1 (MQ=255)
                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTATGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:1263016/140‑1 (MQ=255)
                                                       gTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:195809/140‑1 (MQ=255)
                                                       gTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:5998939/140‑1 (MQ=255)
                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  2:661602/136‑1 (MQ=255)
                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 >  1:661602/1‑136 (MQ=255)
                                                          gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCAAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                           <  1:1780546/140‑1 (MQ=255)
                                                           cGGCAACAATTCAATGAGCGAATTCTCAGGATTCGCCGCGAACTGCGCCAGCCTTGATGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGCCCGCTGATCGCCGCCAGCAGCGGACGcccgg                                                                          >  2:105648/1‑137 (MQ=255)
                                                             gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGTAATCGAGCTGCTGTCCGCTGATGTCCGCCAGCATCTGACGCCGGTAc                                                                        >  2:5846334/1‑140 (MQ=255)
                                                                          taGCGATTTCTCTGGATTCGCAGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGTTAATCGAGCTGCTTAACGCTGATATCCGCCAGCATCTGACGCCATTACTCACTACGcgccc                                                           <  2:1432569/139‑1 (MQ=255)
                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGCGCGGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  2:1432552/140‑1 (MQ=255)
                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  2:4389696/140‑1 (MQ=255)
                                                                             cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgc                                                        >  1:5349298/1‑140 (MQ=255)
                                                                             cGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAACCCCCCCCC‑‑TACGGCAATCGCGCGGCGGACCGCTGAGCTCCGCCAGCATCTGACGCCAGTACTCACGACGcgccccgc                                                        <  2:282811/140‑1 (MQ=255)
                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                       <  1:6366134/140‑1 (MQ=255)
                                                                               cgtactctCGAGTCGCCCCGATCTGCGCCCGCCTTGCAGCCCCCAGTGCGGGCCCCCCAT‑‑CCCCCCACGAACGGCAATCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACGCCAGTACACACCACCcgccccgccc                                                      <  2:5762075/136‑1 (MQ=255)
                                                                               aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgccc                                                      <  2:5761858/140‑1 (MQ=255)
                                                                                tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGCGCTGCTGAGCGCTGATATCCGCCAGCATCTGACGCGAGTACTCACTACGcgccccgccgc                                                     >  1:1192203/1‑138 (MQ=255)
                                                                                  tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                   <  1:2031714/140‑1 (MQ=255)
                                                                                  tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                   <  1:2031744/140‑1 (MQ=255)
                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:3461363/140‑1 (MQ=255)
                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:4199401/140‑1 (MQ=255)
                                                                                    tctGGATTCGCCGCGATATGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:1076908/140‑1 (MQ=255)
                                                                                      tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGGTGACCGCTGTTATCCGCCCGCATCTGACGCCAGTACTCACGACGCGGCCCGCCCCCAATCa                                               >  2:2311624/1‑140 (MQ=255)
                                                                                        gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGGACGGTACTCGAGCTGCTGGCCGCTGATATCCGCCAGCCTCTGACGCCAGTCCGCACTACGCGCCCCGccccccagcgac                                             >  1:1287744/1‑133 (MQ=255)
                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGGACTCGAGCGGCGGACCGCTGATCTGCGCCCGCGTCTGTCGCCCGTTCTCCCTACGCGGCCCGCCCCCAGTCGACGGAac                                        >  1:2195079/1‑140 (MQ=255)
                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGCCGGTAATCGAGCTGCTGACCGCGGGTTTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCGCTCCACGTacg                                        >  1:2367067/1‑138 (MQ=255)
                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACggacc                                        >  1:1118548/1‑136 (MQ=255)
                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTGCGGTAAGCGGGCTGCTGACCGCTGATGTCCGCCAGCATCTGACGCCAGTCCGCACTACGCGCCCCGCCCCCAATCAACGTCac                                        >  1:1478134/1‑140 (MQ=255)
                                                                                             gcagcgATCTGCGCCAGCCTTGCAGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTAGGGAAATGGAGCTGCTGACCGCTGATATACGCCAGCAACTGACGCCAGTACTCACTACGCGCCCCGCCCCCAGTCAACGTAac                                        >  2:2858680/4‑140 (MQ=255)
                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGGTGATATCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCAGTCAACGTAacg                                       >  1:3952984/1‑139 (MQ=255)
                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATGGAGCTGCTGTGCGCTGTTATGCGCCTGCATCTGTCGCCTGGACTCACTACGCGCCCCGCGCCCAATCAACGTAACACttt                                   >  2:3762699/1‑140 (MQ=255)
                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCAACGGAACACttt                                   >  1:2359511/1‑140 (MQ=255)
                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  >  1:555087/1‑140 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGGTGCTGACCGCTGCTATGGGGCAGCATCTGACGCCCGTGGTCACTACGCGCCCCGCCCGCAAGCGACGGAacggttgg                                  >  2:2205178/1‑134 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:2564490/140‑1 (MQ=255)
                                                                                                   aTCTGCGCCAGCCGTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGGAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTACCACTTTg                                  <  2:6068008/140‑1 (MQ=255)
                                                                                                           cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCGGCTGACCGCTGATATCCGCCAGCTTCTGACGCCAGTACTCACTTCGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                          <  2:2179825/140‑1 (MQ=255)
                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCC‑ATCAACGTACCACTTTGCGGTTTAATAcc                      >  2:1054952/1‑140 (MQ=255)
                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAttc                       >  1:984466/1‑138 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGGTTAATACCGCa                   >  2:3764061/1‑140 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGTTTTCGGCCTGCATCTGACGCCCGGACTCACTACGCGCCCCGCCCCCAAGCAACGTAACACGTTGCGGTTTAAGACCGCa                   >  1:1464569/1‑140 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGTAATCGAGCTGGTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa                   >  2:3347760/1‑140 (MQ=255)
                                                                                                                   ctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCACCCGTACGGTAATCGAGCTGATGACCGCTGATATCCGCCAGCAACTGACTCCAGTACTCACTACGCGCACCGCCCCCAATCAACTTAACACTTTGCGTTTTAATACCTCAg                  <  2:3863486/140‑1 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCCCAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  2:222717/140‑1 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  1:594662/140‑1 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  1:4333150/140‑1 (MQ=255)
                                                                                                                           aGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGGACTCGTGCTGCTGGCCGCGGATCTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTGGCGGTTTAATACCGCAGGCATGCAc          >  1:6242713/1‑140 (MQ=255)
                                                                                                                           aGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAAGACCGCAGGCATGccc          <  2:4936049/140‑3 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGTGCTGCTGACCGCTGATATCCGCCAGCATATGACGCCAGTACTCACTCCGCGCCCCGCCCCCATTCAACGTAACACTTTGCGGGTTATTGCCGCAGGCATGCACGa        >  1:1899180/1‑140 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGGTATCCGCCAGCATCTGTCGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTAATACCGCAGGCATGCACGa        >  1:2349610/1‑140 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCGGATATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa        >  1:2349466/1‑140 (MQ=255)
                                                                                                                               cgggCCCCACAT‑‑CCCCCCCCGTACGGTAATCGTGCTGCTGACCGCGGTTATCCGCCAGCATATGACGCCAGTACTCACTACGCGCCCCGCCCCGATTCAACGGACCACTTTGCGGTTTAATACCGCAGGCATGCACGACa      <  2:1548436/138‑1 (MQ=255)
                                                                                                                                 ggCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATc    >  1:3132526/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTACTCGCGCGGCTGTCCGCTGATAGCCGCCAGCAGGTGACGCCTGGACTCACTACGCGCCCCGCCCCCACTCAACGGAACGCTTTGCGGGTTAATACCGCAGGCATGCACGACCTcc   >  2:6429823/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCAGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATca   >  2:2706091/1‑139 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc   >  2:1468584/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAAACCGCAGGAATGCACGACATcc   >  2:2432287/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCGGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTAACACATTGCGGTTTAATACCGCAGGCATGCACGACATcc   >  2:525557/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCGGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTTCGCGCCCCGCCCCCAAGCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc   >  2:2632198/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc   >  1:449752/1‑140 (MQ=255)
                                                                                                                                   ccccACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa  <  2:3402561/140‑1 (MQ=255)
                                                                                                                                           ||                                                                                                                                    
GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  NZ_CP025268/3728668‑3728958
                                                                                                                                            |                                                                                                                                                      
AAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATC                                                                                                                                            >  SRR14920171.2695590/1‑151 (MQ=60)
 AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCG                                                                                                                                           <  SRR14920171.66630/151‑1 (MQ=60)
   CGGCATAACGCTGCGCATCTGGAAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGAACGGGAATCGAG                                                                                                                                         >  SRR14920171.849721/1‑151 (MQ=60)
    GGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGC                                                                                                                                        <  SRR14920171.2051741/151‑1 (MQ=60)
       ATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGC                                                                                                                                     <  SRR14920171.604549/151‑1 (MQ=60)
                 GCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGA                                                                                                                           <  SRR14920171.2164452/151‑1 (MQ=60)
                 GCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGA                                                                                                                           <  SRR14920171.2163167/151‑1 (MQ=60)
                              GCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAT                                                                                                              <  SRR14920171.5290095/151‑1 (MQ=60)
                                    GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CGCCCCCCGGAGGGTAATCGAGCTGCTGACCGCTGATTTCCGCCAGGTTCTGACG                                                                                                        >  SRR14920171.4215646/1‑151 (MQ=60)
                                    GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCGGATATCCGCCCGCCGCTGACG                                                                                                        >  SRR14920171.2810066/1‑151 (MQ=60)
                                    GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACG                                                                                                        >  SRR14920171.2809631/1‑151 (MQ=60)
                                    GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGGCATCGAGCTGGGGACCGCTGATGTCCGGCAGCATCTGACG                                                                                                        >  SRR14920171.6099076/1‑151 (MQ=60)
                                      TCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCC                                                                                                      >  SRR14920171.664581/1‑151 (MQ=60)
                                               AGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAC                                                                                             <  SRR14920171.6117784/151‑1 (MQ=60)
                                                   GCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCAAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACG                                                                                         <  SRR14920171.1788547/151‑1 (MQ=60)
                                                           AATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGC                                                                                 >  SRR14920171.5373916/1‑151 (MQ=60)
                                                              TCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGCGCTGCTGAGCGCTGATATCCGCCAGCATCTGACGCGAGTACTCACTACGCGCCCCGCCGC                                                                              >  SRR14920171.1197675/1‑151 (MQ=60)
                                                                      CGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGGACGGTACTCGAGCTGCTGGCCGCTGATATCCGCCAGCCTCTGACGCCAGTCCGCACTACGCGCCCCGCCCCCcagcgac                                                                      >  SRR14920171.1293632/1‑144 (MQ=60)
                                                                       GATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACG                                                                     <  SRR14920171.6397716/151‑1 (MQ=60)
                                                                           TCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAAC                                                                 <  SRR14920171.2040994/151‑1 (MQ=60)
                                                                           TCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAAC                                                                 <  SRR14920171.2041025/151‑1 (MQ=60)
                                                                           TCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGCCGGTAATCGAGCTGCTGACCGCGGGTTTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCgctccacgtacg                                                                 >  SRR14920171.2377825/1‑139 (MQ=60)
                                                                           TCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACGgacc                                                                 >  SRR14920171.1123674/1‑147 (MQ=60)
                                                                           TCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTGCGGTAAGCGGGCTGCTGACCGCTGATGTCCGCCAGCATCTGACGCCAGTCCGCACTACGCGCCCCGCCCCCAATCAACGTCAC                                                                 >  SRR14920171.1484859/1‑151 (MQ=60)
                                                                            CTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGGTGATATCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCAGTCAACGTAACG                                                                >  SRR14920171.3971108/1‑151 (MQ=60)
                                                                             TCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACAC                                                               <  SRR14920171.4218607/151‑1 (MQ=60)
                                                                                GGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCAACGGAACACTTT                                                            >  SRR14920171.2370234/1‑151 (MQ=60)
                                                                                GGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTG                                                           >  SRR14920171.557569/1‑151 (MQ=60)
                                                                                            ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAC                                                <  SRR14920171.2576142/151‑1 (MQ=60)
                                                                                            ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATTC                                                >  SRR14920171.988931/1‑151 (MQ=60)
                                                                                            ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGTAATCGAGCTGCTGAGCGCTGATATGCGCCAGCATCTGACGGCAGTACGGACTACGCGCCCCGCCCCCCATCAACGTAACACGTTGCGGggttattg                                                >  SRR14920171.2673553/1‑143 (MQ=60)
                                                                                                GCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGTTTTCGGCCTGCATCTGACGCCCGGACTCACTACGCGCCCCGCCCCCAAGCAACGTAACACGTTGCGGTTTAAGACCGCA                                            >  SRR14920171.1471236/1‑151 (MQ=60)
                                                                                                         TTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGGACTCGTGCTGCTGGCCGCGGATCTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTGGCGGTTTAATACCGCAGGCATGCAC                                   >  SRR14920171.6273533/1‑151 (MQ=60)
                                                                                                           GCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGTGCTGCTGACCGCTGATATCCGCCAGCATATGACGCCAGTACTCACTCCGCGCCCCGCCCCCATTCAACGTAACACTTTGCGGGTTATTGCCGCAGGCATGCACGA                                 >  SRR14920171.1907805/1‑151 (MQ=60)
                                                                                                           GCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGGTATCCGCCAGCATCTGTCGCCAGTACTCACGACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGGTTGATACCGCAGGCATGCACGA                                 >  SRR14920171.2360291/1‑151 (MQ=60)
                                                                                                           GCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCGGATATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTGTAATACCGCAGGCATGCACGA                                 >  SRR14920171.2360144/1‑151 (MQ=60)
                                                                                                               CGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATC                             >  SRR14920171.3146823/1‑151 (MQ=60)
                                                                                                               CGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATC                             <  SRR14920171.4352941/151‑1 (MQ=60)
                                                                                                               CGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATC                             <  SRR14920171.597343/151‑1 (MQ=60)
                                                                                                                GCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC                            >  SRR14920171.451773/1‑151 (MQ=60)
                                                                                                                     GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920171.5379511/1‑151 (MQ=60)
                                                                                                                     GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTCACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920171.374258/1‑151 (MQ=60)
                                                                                                                     GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGCCATCCATGCC                       >  SRR14920171.2660674/1‑151 (MQ=60)
                                                                                                                     GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920171.2522773/1‑151 (MQ=60)
                                                                                                                     GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCCGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCC                       >  SRR14920171.1770103/1‑151 (MQ=60)
                                                                                                                            CCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCC                >  SRR14920171.1144088/1‑151 (MQ=60)
                                                                                                                             CCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCA               >  SRR14920171.73287/1‑151 (MQ=60)
                                                                                                                             CCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCA               >  SRR14920171.2817117/1‑151 (MQ=60)
                                                                                                                                  acatCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCG            >  SRR14920171.945280/5‑151 (MQ=60)
                                                                                                                                   catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920171.68728/4‑151 (MQ=60)
                                                                                                                                   catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920171.68427/4‑151 (MQ=60)
                                                                                                                                   catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920171.1718506/4‑151 (MQ=60)
                                                                                                                                   catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920171.5447200/4‑151 (MQ=60)
                                                                                                                                   catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC           >  SRR14920171.3505470/4‑151 (MQ=60)
                                                                                                                                    atCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCA          >  SRR14920171.394947/3‑151 (MQ=60)
                                                                                                                                     TCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACActgtctcttatacacatctccg         >  SRR14920171.2116097/1‑129 (MQ=60)
                                                                                                                                     TCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAT         <  SRR14920171.5016341/151‑1 (MQ=60)
                                                                                                                                       CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAA       >  SRR14920171.6384052/1‑151 (MQ=60)
                                                                                                                                         CACCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAACTCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACC     >  SRR14920171.3770704/1‑151 (MQ=60)
                                                                                                                                           CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA   >  SRR14920171.1131969/1‑151 (MQ=60)
                                                                                                                                           CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA   >  SRR14920171.4280550/1‑151 (MQ=60)
                                                                                                                                            CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  SRR14920171.5225677/1‑151 (MQ=60)
                                                                                                                                            CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  SRR14920171.3725217/1‑151 (MQ=60)
                                                                                                                                            CCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  SRR14920171.1561683/1‑151 (MQ=60)
                                                                                                                                            |                                                                                                                                                      
AAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  NZ_CP025268/3728668‑3728958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑