Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
148 |
28.0 |
2229078 |
88.9% |
1981650 |
107.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,905,524 |
G→C |
A1285G (GCC→GGC) |
putA ← |
bifunctional proline dehydrogenase and proline oxidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,905,524 | 0 | G | C | 100.0%
| 157.0
/ NA
| 41 | A1285G (GCC→GGC) | putA | bifunctional proline dehydrogenase and proline oxidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (18/23); total (18/23) |
ACGAGGTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGGCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTC > AM260479/3905393‑3905637
|
aCGAGGTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGccc < 2:1032496/134‑1 (MQ=255)
ggTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCa < 2:1040357/134‑1 (MQ=255)
gTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCAc > 1:196998/1‑134 (MQ=255)
aGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCga < 1:858895/134‑1 (MQ=255)
cAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGAcc > 1:496885/1‑134 (MQ=255)
aTCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGATCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGgcg > 2:697557/1‑134 (MQ=255)
tcatcaAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCAcc > 2:829844/1‑134 (MQ=255)
catcaAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCg > 2:784190/1‑134 (MQ=255)
catcaAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCg > 1:918360/1‑134 (MQ=255)
atcaAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGc < 2:196998/134‑1 (MQ=255)
gCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTc < 2:496885/134‑1 (MQ=255)
gCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGATCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGc < 1:697557/132‑1 (MQ=255)
tGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGca > 2:365185/1‑133 (MQ=255)
tGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGca > 1:907070/1‑133 (MQ=255)
gccgccCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGc > 1:483077/1‑120 (MQ=255)
gccgccCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGc < 2:483077/120‑1 (MQ=255)
gcccgcGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCg < 2:446322/134‑1 (MQ=255)
ggTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGt < 1:829844/134‑1 (MQ=255)
aCCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAAtc < 1:838197/134‑1 (MQ=255)
aCCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAAtc < 1:749571/134‑1 (MQ=255)
cGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAAtctc < 1:971564/134‑1 (MQ=255)
gagcgCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCgtggtg > 1:807187/1‑134 (MQ=255)
gcTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTgcagc < 2:657891/134‑1 (MQ=255)
cTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTgcagca < 1:784190/134‑1 (MQ=255)
cTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTgcagca < 2:573091/134‑1 (MQ=255)
cGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCgtgg < 2:302775/125‑1 (MQ=255)
cGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCgtgg > 1:302775/1‑125 (MQ=255)
cgATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCg > 2:478632/1‑71 (MQ=255)
cgATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCg < 1:478632/71‑1 (MQ=255)
atatTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCcgccgc < 1:525472/134‑1 (MQ=255)
cGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCgt > 2:221126/1‑95 (MQ=255)
cGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCgt < 1:221126/95‑1 (MQ=255)
tGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGc > 1:701279/1‑44 (MQ=255)
tGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGc < 1:680227/44‑1 (MQ=255)
tGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGc > 2:680227/1‑44 (MQ=255)
tGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGc < 2:701279/44‑1 (MQ=255)
tGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGc > 2:273068/1‑51 (MQ=255)
tGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGc < 1:273068/51‑1 (MQ=255)
tGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCg > 2:356955/1‑54 (MQ=255)
tGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCg < 1:356955/54‑1 (MQ=255)
cAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTc > 1:150088/1‑118 (MQ=255)
cAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTc < 2:150088/118‑1 (MQ=255)
|
ACGAGGTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGGCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTC > AM260479/3905393‑3905637
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CTTCGACATGACGAGGTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGGCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTCCAGTCGGCCACCATGCGGA > AM260479/3905383‑3905656
|
CTTCGACATGACGAGGTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACG > GWNJ‑0478:712:GW2002102894th:2:1113:14787:89254/1‑150 (MQ=60)
GACATGACGAGGTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGA < GWNJ‑0478:712:GW2002102894th:2:1204:14697:74635/150‑1 (MQ=60)
GTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCG > GWNJ‑0478:712:GW2002102894th:2:1215:20282:11411/1‑150 (MQ=60)
GAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGC < GWNJ‑0478:712:GW2002102894th:2:2203:5929:86067/150‑1 (MQ=60)
GCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGC > GWNJ‑0478:712:GW2002102894th:2:2209:17062:50558/1‑150 (MQ=60)
TCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCAC > GWNJ‑0478:712:GW2002102894th:2:2205:10541:87213/1‑149 (MQ=60)
CATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACAC > GWNJ‑0478:712:GW2002102894th:2:1212:18958:39994/1‑150 (MQ=60)
tttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACAC < GWNJ‑0478:712:GW2002102894th:2:2108:17842:95935/75‑1 (MQ=60)
ttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGC < GWNJ‑0478:712:GW2002102894th:2:1201:7027:4012/82‑1 (MQ=60)
GGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGATCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGC < GWNJ‑0478:712:GW2002102894th:2:2109:14623:94298/148‑1 (MQ=60)
caagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGC < GWNJ‑0478:712:GW2002102894th:2:1207:14451:35929/85‑1 (MQ=60)
acgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGC < GWNJ‑0478:712:GW2002102894th:2:1212:13149:16342/101‑1 (MQ=60)
CGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGT < GWNJ‑0478:712:GW2002102894th:2:2116:18020:97437/150‑1 (MQ=60)
GTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGC > GWNJ‑0478:712:GW2002102894th:2:2114:13201:78719/1‑150 (MQ=60)
GACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCAC < GWNJ‑0478:712:GW2002102894th:2:2201:18332:52870/150‑1 (MQ=60)
GACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCAC < GWNJ‑0478:712:GW2002102894th:2:2113:7524:78170/150‑1 (MQ=60)
CCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCG > GWNJ‑0478:712:GW2002102894th:2:1203:5616:95316/1‑150 (MQ=60)
CCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCG < GWNJ‑0478:712:GW2002102894th:2:2208:9506:61205/150‑1 (MQ=60)
gctcggagatgtgtataagagacaGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCAT < GWNJ‑0478:712:GW2002102894th:2:1115:14569:20287/126‑1 (MQ=60)
GCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGA < GWNJ‑0478:712:GW2002102894th:2:2115:15536:60799/150‑1 (MQ=60)
GATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTCCAGTCGGCCAC < GWNJ‑0478:712:GW2002102894th:2:1214:10367:53530/150‑1 (MQ=60)
ATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2111:1391:15862/1‑75 (MQ=60)
CGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTCCAGTCGGCCACCATGCT > GWNJ‑0478:712:GW2002102894th:2:1111:19984:30830/1‑150 (MQ=60)
GTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCtgtctcttatacacatctccgagcccacgagacactcgctaatctc > GWNJ‑0478:712:GW2002102894th:2:1212:20105:91880/1‑104 (MQ=60)
GTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCtgtctcttatacacatctccgagcccacgagacactcgctaatctc > GWNJ‑0478:712:GW2002102894th:2:1212:15202:92877/1‑104 (MQ=60)
GTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCtgtctcttatacacatctccgagcccacgagacactcgctaatctc > GWNJ‑0478:712:GW2002102894th:2:1110:11607:34376/1‑104 (MQ=60)
GTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTCCAGTCGGCCACCATGCGG > GWNJ‑0478:712:GW2002102894th:2:1103:13734:97546/1‑149 (MQ=60)
|
CTTCGACATGACGAGGTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGGCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTCCAGTCGGCCACCATGCGGA > AM260479/3905383‑3905656
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
