Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,112,158 |
Δ1 bp |
coding (2295/2469 nt) |
xdhB1 ← |
xanthine dehydrogenase, subunit B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,112,156 | 0 | C | . | 100.0%
| 95.2
/ NA
| 22 | coding (2297/2469 nt) | xdhB1 | xanthine dehydrogenase, subunit B |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (9/13); total (9/13) |
TCAACGCGGCCAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCGCCCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAACACCGAGAACGGCAGCAGCAGCGGCGGCTCGCCCACGGC > AM260479/1112033‑1112277
|
tCAACGCGGCCAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCtggtg < 2:654222/134‑1 (MQ=255)
cgcgGCCAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCg > 1:461543/1‑134 (MQ=255)
ggCCAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCgggg < 1:809072/134‑1 (MQ=255)
cAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCt < 2:61273/134‑1 (MQ=255)
ggggggAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCtt > 1:166541/1‑132 (MQ=255)
gggggAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAg < 2:166541/134‑1 (MQ=255)
gAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAgcggc < 1:679505/134‑1 (MQ=255)
ggggCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATg > 2:11764/1‑134 (MQ=255)
gcgcCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGtt > 1:245997/1‑127 (MQ=255)
gcgcCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGtt < 2:245997/127‑1 (MQ=255)
cAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGc > 1:162247/1‑134 (MQ=255)
tCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTcg < 1:339510/134‑1 (MQ=255)
gTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTcgcg < 1:409290/134‑1 (MQ=255)
ccGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGa < 2:763431/133‑1 (MQ=255)
ccGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGAt > 1:763431/1‑134 (MQ=255)
ccAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGt < 2:1032569/124‑1 (MQ=255)
ccAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGt > 1:1032569/1‑124 (MQ=255)
aGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCga < 2:162247/134‑1 (MQ=255)
cGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAACACCGAGAACGGCAGCAGCAGCGGCGGCTCg < 1:11764/134‑1 (MQ=255)
tCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAACACCGAGAACGGCAGCAGCAGCGGCGGCTCGcc > 2:221679/1‑134 (MQ=255)
cGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAACACCGAGAACGGCAGCAGCAGCGGCGGCTCGcc > 2:498613/1‑133 (MQ=255)
gCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAACACCGAGAACGGCAGCAGCAGCGGCGGCTCGCCCACGGc < 2:461543/134‑1 (MQ=255)
|
TCAACGCGGCCAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCGCCCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAACACCGAGAACGGCAGCAGCAGCGGCGGCTCGCCCACGGC > AM260479/1112033‑1112277
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TCCACCGGCCCCATGCCCGTCAACGCGGCCAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCGCCCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAACACCGAGAACGGCAGCAGCAGCGGCGGCTCGCCCACGGCCTTGGAGCGGTGGATGCTGTCC > AM260479/1112014‑1112299
|
TCCACCGGCCCCATGCCCGTCAACGCGGCCAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGC < GWNJ‑0478:712:GW2002102894th:2:1203:10572:89396/148‑1 (MQ=60)
ggtctttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCG < GWNJ‑0478:712:GW2002102894th:2:1109:5183:83312/67‑1 (MQ=60)
CCCCATGCCCGTCAACGCGGCCAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGG > GWNJ‑0478:712:GW2002102894th:2:1212:2073:69709/1‑150 (MQ=60)
CGTCAACGCGGCCAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTC > GWNJ‑0478:712:GW2002102894th:2:1110:3434:82738/1‑148 (MQ=60)
CGGCCAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGT < GWNJ‑0478:712:GW2002102894th:2:2202:7848:17780/150‑1 (MQ=60)
GGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGT > GWNJ‑0478:712:GW2002102894th:2:1116:14993:25650/1‑150 (MQ=60)
GGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAG < GWNJ‑0478:712:GW2002102894th:2:2110:4116:38508/150‑1 (MQ=60)
GTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCC > GWNJ‑0478:712:GW2002102894th:2:1110:13979:56747/1‑150 (MQ=60)
CTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATC > GWNJ‑0478:712:GW2002102894th:2:2116:9556:14374/1‑150 (MQ=60)
CCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCG > GWNJ‑0478:712:GW2002102894th:2:2216:10729:52172/1‑150 (MQ=60)
ATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAAC < GWNJ‑0478:712:GW2002102894th:2:1207:20902:59249/150‑1 (MQ=60)
CGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAACAC < GWNJ‑0478:712:GW2002102894th:2:1211:11535:79313/150‑1 (MQ=60)
GCGTCGACGGCGTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAACACCGAGAACGGCAGCAGCAGCGGCGGCTCGCCCACGGCCTTGGAG < GWNJ‑0478:712:GW2002102894th:2:1101:9179:86630/150‑1 (MQ=60)
GTCCAGGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAACACCGAGAACGGCctgtctcttatacacatctccgagcccacgagacggagctacat > GWNJ‑0478:712:GW2002102894th:2:2205:17816:80913/1‑106 (MQ=60)
GGATCG‑CCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCtgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaa > GWNJ‑0478:712:GW2002102894th:2:1108:19240:35827/1‑80 (MQ=60)
|
TCCACCGGCCCCATGCCCGTCAACGCGGCCAAGGGGGGAAAGTCCGGGGGCGCCCGCAGGCGCGCGCTTCCTGCGATCGTTCCGCCAGCTCAGGCTGGCTGCGCCGCGGCTTCGCGCACGGCGTCGACGGCGTCCAGGATCGCCCTCGCTGGTGGCCGGGGCCTTCAGCGGCGGGTTGATGCGGTAGTCGCCAACGGCAGCCACGGCGTCGCGGATCGCGAAGAACACCGAGAACGGCAGCAGCAGCGGCGGCTCGCCCACGGCCTTGGAGCGGTGGATGCTGTCC > AM260479/1112014‑1112299
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
