Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,253,193 |
+C |
coding (243/258 nt) |
h16_A2071 → |
Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,253,191 | 1 | . | C | 100.0%
| 60.1
/ NA
| 17 | T81P (ACC→CCC) | h16_A2071 | Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (6/11); total (6/11) |
CGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGG > AM260479/2253075‑2253251
|
cGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTgg > 2:532921/1‑132 (MQ=255)
cTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGAt < 1:532921/134‑1 (MQ=255)
cACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATc < 1:992060/132‑1 (MQ=255)
gCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTAc < 1:66190/134‑1 (MQ=255)
cgccgGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGAt < 1:41396/114‑1 (MQ=255)
cgccgGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGAt > 2:41396/1‑114 (MQ=255)
tCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCa < 1:167185/134‑1 (MQ=255)
gcgcAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAgg < 1:749955/134‑1 (MQ=255)
gcgcAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAgg < 1:457124/134‑1 (MQ=255)
cGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCgaga < 1:464990/90‑1 (MQ=255)
cGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCgaga > 2:464990/1‑90 (MQ=255)
aacaGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCt > 1:1019426/1‑50 (MQ=255)
aacaGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCt < 2:1019426/50‑1 (MQ=255)
ttCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATc < 1:753968/57‑1 (MQ=255)
ttCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGAGCCCGCTGGTAGCGGCCGAGATc > 2:753968/1‑57 (MQ=255)
cGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGc < 2:335730/73‑1 (MQ=255)
cGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGc > 1:335730/1‑73 (MQ=255)
|
CGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGG > AM260479/2253075‑2253251
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCATCACCTGGCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCGTGACCCCCAGCAACACCCGCCTCCCCGCATCGCCCGACGCCCTGCCCGCCCATGCCCCGGCAGCGCACGCCGCG > AM260479/2253051‑2253340
|
gcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCC < GWNJ‑0478:712:GW2002102894th:2:2215:5949:76815/97‑1 (MQ=60)
agacagCGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGA < GWNJ‑0478:712:GW2002102894th:2:1105:16422:61219/144‑1 (MQ=60)
CCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGA < GWNJ‑0478:712:GW2002102894th:2:1215:17080:93109/150‑1 (MQ=60)
gcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGAC < GWNJ‑0478:712:GW2002102894th:2:2110:14814:80464/87‑1 (MQ=60)
GCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGAC < GWNJ‑0478:712:GW2002102894th:2:2208:10075:30476/148‑1 (MQ=60)
CGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCC < GWNJ‑0478:712:GW2002102894th:2:1104:7743:20622/150‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacaGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGC < GWNJ‑0478:712:GW2002102894th:2:1210:8038:59731/121‑1 (MQ=60)
GTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGC < GWNJ‑0478:712:GW2002102894th:2:1111:1671:96282/150‑1 (MQ=60)
CGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCG < GWNJ‑0478:712:GW2002102894th:2:1210:12302:18532/150‑1 (MQ=60)
CGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCG < GWNJ‑0478:712:GW2002102894th:2:2110:4490:58468/150‑1 (MQ=60)
GGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaac > GWNJ‑0478:712:GW2002102894th:2:2214:11683:94493/1‑70 (MQ=60)
TCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaa > GWNJ‑0478:712:GW2002102894th:2:2211:7867:81910/1‑81 (MQ=60)
TGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCtgtctcttatacacatctccgagcccacgagacactcgctaatctc > GWNJ‑0478:712:GW2002102894th:2:1205:18265:37569/1‑104 (MQ=60)
C‑CCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCGTGACCCCCAGCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgc > GWNJ‑0478:712:GW2002102894th:2:1205:19167:58408/1‑87 (MQ=60)
C‑CCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCGTGACCCCCAGCAACACCCGCCTCCCCGCATCGCCCGACGCCCTGCCCctgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:2107:13772:85615/1‑123 (MQ=60)
|
CCATCACCTGGCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCGTGACCCCCAGCAACACCCGCCTCCCCGCATCGCCCGACGCCCTGCCCGCCCATGCCCCGGCAGCGCACGCCGCG > AM260479/2253051‑2253340
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
