Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,253,193 |
+C |
coding (243/258 nt) |
h16_A2071 → |
Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,253,191 | 1 | . | C | 100.0%
| 54.0
/ NA
| 15 | T81P (ACC→CCC) | h16_A2071 | Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (5/10); total (5/10) |
GCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCGTGACCCC > AM260479/2253061‑2253273
|
gCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑ccc < 2:994397/134‑2 (MQ=255)
gCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑ccc < 2:464972/134‑2 (MQ=255)
aCCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑ccc < 1:772544/118‑2 (MQ=255)
aCCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑ccc > 2:772544/1‑117 (MQ=255)
cTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGAt < 1:915772/134‑1 (MQ=255)
cACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATc < 1:62878/132‑1 (MQ=255)
cgccgGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTAcg > 2:550625/1‑134 (MQ=255)
gCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGcc < 1:843619/134‑1 (MQ=255)
ccACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATc < 1:777417/109‑1 (MQ=255)
ccACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATc > 2:777417/1‑109 (MQ=255)
tGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATc > 1:1028049/1‑95 (MQ=255)
tGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATc < 2:1028049/95‑1 (MQ=255)
ccTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGaa < 1:550625/134‑1 (MQ=255)
tgCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGcc < 2:460795/133‑1 (MQ=255)
ttCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCg > 1:152590/1‑73 (MQ=255)
ttCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCg < 2:152590/73‑1 (MQ=255)
tcctccGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTA‑‑GGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCGTGAcccc < 1:466871/133‑1 (MQ=255)
ttCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTa < 1:941387/65‑1 (MQ=255)
ttCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTa > 2:941387/1‑65 (MQ=255)
|
GCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCGTGACCCC > AM260479/2253061‑2253273
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCCATCACCTGGCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCGTGACCCCCAGCAACACCCGCCTCCCCGCATCGCCCGACGCCCTGCCCGCCCATGCCCCGGCAGCGC > AM260479/2253050‑2253332
|
CCCATCACCTGGCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CCctgtct > GWNJ‑0478:712:GW2002102894th:2:2102:11294:95923/1‑144 (MQ=60)
AGCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAG < GWNJ‑0478:712:GW2002102894th:2:2116:4184:66936/150‑1 (MQ=60)
CCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGA < GWNJ‑0478:712:GW2002102894th:2:2211:16809:6583/150‑1 (MQ=60)
ataagagacaGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGAC < GWNJ‑0478:712:GW2002102894th:2:2116:8271:96016/140‑1 (MQ=60)
GCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGAC < GWNJ‑0478:712:GW2002102894th:2:1104:9879:38218/148‑1 (MQ=60)
cggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGC < GWNJ‑0478:712:GW2002102894th:2:2210:3074:54199/95‑1 (MQ=60)
GGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGT < GWNJ‑0478:712:GW2002102894th:2:2205:3630:53776/150‑1 (MQ=60)
caagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGT < GWNJ‑0478:712:GW2002102894th:2:2101:19480:100519/83‑1 (MQ=60)
GCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACctgtctcttatacacatctccgagc > GWNJ‑0478:712:GW2002102894th:2:2216:14634:29277/1‑125 (MQ=60)
GGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGctgtctcttatacacatctccgagcccacgagacggagctacatctc > GWNJ‑0478:712:GW2002102894th:2:1111:6779:99389/1‑103 (MQ=60)
GCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCGTGACCCCCAGC < GWNJ‑0478:712:GW2002102894th:2:2103:16488:95241/150‑1 (MQ=60)
TTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAACCCTGCGCCCGCTGGTA‑‑GGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCGTGACCCCCAGCAACACCCGCCT < GWNJ‑0478:712:GW2002102894th:2:1212:7881:97601/149‑1 (MQ=60)
GTGGCAAACCCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaacattaaataatcgat > GWNJ‑0478:712:GW2002102894th:2:1115:17070:33570/1‑52 (MQ=60)
|
CCCATCACCTGGCAGCCACCGCGGTCGACCCGCATCCTGCACGTACGCACCGATGCCGCCGGCCACGTCGCGCAGGTGCGCCTGTGCATTTCCTCCGATAACAACAACAGCGTGTTCGCTCCGCAGCCGGTGCAGTGGCAAA‑CCTGCGCCCGCTGGTAGCGGCCGAGATCGCGCAGTACCGAGACTACGCGGCCCGAGCAGGCCGCTGTTGAAGCCGTGACCCCCAGCAACACCCGCCTCCCCGCATCGCCCGACGCCCTGCCCGCCCATGCCCCGGCAGCGC > AM260479/2253050‑2253332
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
