Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,972,184 |
+C |
intergenic (‑136/‑395) |
h16_A3682 ← / → h16_A3683 |
putative methyltransferase/Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,972,182 | 1 | . | C | 97.6%
| 151.0
/ ‑3.2
| 41 | intergenic (‑134/‑397) | h16_A3682/h16_A3683 | putative methyltransferase/Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (19/21); total (19/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 |
TTCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑G‑CCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATT > AM260479/3972055‑3972295
|
ttCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑G‑ccctgg > 1:236651/1‑130 (MQ=255)
cGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTg < 2:236651/134‑1 (MQ=255)
cTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGAt < 1:827549/134‑1 (MQ=255)
gtcgCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTg < 1:396913/133‑1 (MQ=255)
tttGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGtat < 2:252880/133‑1 (MQ=255)
ccggccgTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAg > 2:766947/1‑134 (MQ=255)
gccgTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcg < 2:160234/134‑1 (MQ=255)
gcgcGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGAt < 2:508587/89‑1 (MQ=255)
gcgcGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGAt > 1:508587/1‑89 (MQ=255)
gcgcGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCg > 1:1135662/1‑134 (MQ=255)
ggATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑G‑ccctg < 2:426544/71‑4 (MQ=255)
ggATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑G‑ccctg > 1:426544/1‑68 (MQ=255)
gTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACTC‑CCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGAt < 2:71198/134‑1 (MQ=255)
gTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGAt < 2:85986/134‑1 (MQ=255)
cacGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCg < 2:759092/134‑1 (MQ=255)
cGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACtgtg > 1:256678/1‑133 (MQ=255)
gTAACACCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGa > 1:664927/1‑134 (MQ=255)
cacCCCTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAg < 1:766947/134‑1 (MQ=255)
ccccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGAt < 2:190532/41‑1 (MQ=255)
ccccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGAt > 1:190532/1‑41 (MQ=255)
ccccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg < 1:268907/79‑1 (MQ=255)
ccccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg > 2:268907/1‑79 (MQ=255)
ccccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGcc < 2:64771/84‑1 (MQ=255)
ccccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGcc > 1:64771/1‑84 (MQ=255)
ccccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCa > 1:288217/1‑134 (MQ=255)
ccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGAt < 2:558687/39‑1 (MQ=255)
ccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGAt > 1:558687/1‑39 (MQ=255)
ccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACg > 2:1116049/1‑90 (MQ=255)
ccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACg < 1:1116049/90‑1 (MQ=255)
ccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTcccc > 2:185602/1‑95 (MQ=255)
ccTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTcccc < 1:185602/95‑1 (MQ=255)
cTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg > 2:1102067/1‑76 (MQ=255)
cTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg < 1:1102067/76‑1 (MQ=255)
cTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGc < 2:123088/112‑1 (MQ=255)
cTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGc > 1:123088/1‑112 (MQ=255)
cTTTCCGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCAcgc > 1:463887/1‑134 (MQ=255)
cGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCAccc > 2:146602/1‑52 (MQ=255)
cGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCAccc < 1:146602/52‑1 (MQ=255)
cGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTTGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGAtt > 1:282273/1‑134 (MQ=255)
cGGCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGAt < 2:463887/133‑1 (MQ=255)
ggCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACg > 1:424402/1‑45 (MQ=255)
ggCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACg < 2:424402/45‑1 (MQ=255)
ggCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCAcgc < 2:1003549/128‑1 (MQ=255)
ggCGACCTCACCGTATAC‑GCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCAcgc > 1:1003549/1‑128 (MQ=255)
|
TTCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATAC‑G‑CCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATT > AM260479/3972055‑3972295
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCCTTGTCCGCCATTTGCCTTTCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATGTGGGCTGCCTGGGGCTGGCATGGGGATGCCT > AM260479/3972035‑3972331
|
caagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CC < GWNJ‑0478:712:GW2002102894th:2:2102:13578:3212/84‑1 (MQ=60)
agtgtctcgtgggctcggagatgtgtataagagacagGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CC < GWNJ‑0478:712:GW2002102894th:2:2103:7626:45348/113‑1 (MQ=60)
GCCTTGTCCGCCATTTGCCTTTCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CC < GWNJ‑0478:712:GW2002102894th:2:1104:18356:75640/150‑1 (MQ=60)
atggtaccttgtagtgcttttccttactatttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CC < GWNJ‑0478:712:GW2002102894th:2:1207:4215:4807/43‑1 (MQ=60)
CCTTGTCCGCCATTTGCCTTTCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCC < GWNJ‑0478:712:GW2002102894th:2:1102:8886:41071/150‑1 (MQ=60)
CCTTGTCCGCCATTTGCCTTTCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCC < GWNJ‑0478:712:GW2002102894th:2:2109:1982:92461/150‑1 (MQ=60)
tttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCC < GWNJ‑0478:712:GW2002102894th:2:1107:14859:83182/69‑1 (MQ=60)
gaagtgtttttttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCC < GWNJ‑0478:712:GW2002102894th:2:1214:20216:23693/59‑1 (MQ=60)
GTCCGCCATTTGCCTTTCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGT > GWNJ‑0478:712:GW2002102894th:2:1115:17263:58527/1‑150 (MQ=60)
GCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGC < GWNJ‑0478:712:GW2002102894th:2:2114:18301:57815/150‑1 (MQ=60)
CGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCAC < GWNJ‑0478:712:GW2002102894th:2:1206:2182:96566/149‑1 (MQ=60)
tcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGC < GWNJ‑0478:712:GW2002102894th:2:1109:3951:87006/82‑1 (MQ=60)
tatatttttcttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaggtatacgcCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGA < GWNJ‑0478:712:GW2002102894th:2:1109:6623:16979/51‑1 (MQ=60)
GTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCctgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:1212:12940:77945/1‑119 (MQ=60)
GTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGT > GWNJ‑0478:712:GW2002102894th:2:2216:4935:65444/1‑150 (MQ=60)
attagcgagtgtctcgtgggctcggagatgtgtataagagacagGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTC < GWNJ‑0478:712:GW2002102894th:2:2215:2338:6050/106‑1 (MQ=60)
agcgagtgtctcgtgggctcggagatgtgtataagagacagCACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTC < GWNJ‑0478:712:GW2002102894th:2:1116:7696:21501/109‑1 (MQ=60)
ttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaggtatacgcCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTC < GWNJ‑0478:712:GW2002102894th:2:1206:18117:6556/65‑1 (MQ=60)
TTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgt > GWNJ‑0478:712:GW2002102894th:2:1209:20284:58061/1‑101 (MQ=60)
cgttggctcggagatgtgtataagagacagCGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGAT < GWNJ‑0478:712:GW2002102894th:2:2215:21201:72059/120‑1 (MQ=60)
agcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaggtatacgcCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGAT < GWNJ‑0478:712:GW2002102894th:2:2113:12180:84754/78‑1 (MQ=60)
gctcggagatgtgtataagagacagCGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCC < GWNJ‑0478:712:GW2002102894th:2:1110:13631:96931/125‑1 (MQ=60)
ggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaggtatacgcCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCT < GWNJ‑0478:712:GW2002102894th:2:2116:14187:62141/88‑1 (MQ=60)
attagcgagtgtctcgtgggctcggagatgtgtataagagacaggtatacgcCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGG < GWNJ‑0478:712:GW2002102894th:2:1215:15765:76416/98‑1 (MQ=60)
ACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGG > GWNJ‑0478:712:GW2002102894th:2:1114:18512:59729/1‑149 (MQ=60)
CCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGAC > GWNJ‑0478:712:GW2002102894th:2:2107:7422:76545/1‑150 (MQ=60)
CGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaataagctggatccgtcagccccc > GWNJ‑0478:712:GW2002102894th:2:2106:9597:60275/1‑50 (MQ=60)
cgagtgtctcgtgggctcggagatgtgtataagagacaggtatacgcCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAG < GWNJ‑0478:712:GW2002102894th:2:2204:18305:6479/103‑1 (MQ=60)
agtgtctcgtgggctcggagatgtgtataagagacaggtatacgcCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCA < GWNJ‑0478:712:GW2002102894th:2:1211:16613:70346/105‑1 (MQ=60)
CACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1113:2490:23201/1‑71 (MQ=60)
CACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCctgtctcttatacacatctccgagcccacgagacac > GWNJ‑0478:712:GW2002102894th:2:1104:12549:11699/1‑114 (MQ=60)
CACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCAC > GWNJ‑0478:712:GW2002102894th:2:1201:17435:37639/1‑150 (MQ=60)
CACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCcctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaaaaaaaacaacagagaaagtcg > GWNJ‑0478:712:GW2002102894th:2:1211:9633:27944/1‑45 (MQ=60)
CGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaac > GWNJ‑0478:712:GW2002102894th:2:1216:7669:18305/1‑69 (MQ=60)
GTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACActgtctct > GWNJ‑0478:712:GW2002102894th:2:1108:7635:55153/1‑142 (MQ=60)
GTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCG > GWNJ‑0478:712:GW2002102894th:2:1210:5864:54112/1‑150 (MQ=60)
TATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCcctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaatgagccaggcgcaagcgaaatagac > GWNJ‑0478:712:GW2002102894th:2:1110:8041:53729/1‑41 (MQ=60)
ACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaataacaaataaaatgaaaacaacaccgaccttgcaa > GWNJ‑0478:712:GW2002102894th:2:2206:17478:78478/1‑38 (MQ=60)
CGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTTGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTT > GWNJ‑0478:712:GW2002102894th:2:1116:3258:99088/1‑150 (MQ=60)
GTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1209:5114:46633/1‑77 (MQ=60)
GTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGG > GWNJ‑0478:712:GW2002102894th:2:2208:3210:95411/1‑150 (MQ=60)
ACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATG < GWNJ‑0478:712:GW2002102894th:2:2113:20863:49078/150‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacagcCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGA < GWNJ‑0478:712:GW2002102894th:2:1112:13494:36123/116‑1 (MQ=60)
cggagatgtgtataagagacaggtatacgcCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATG < GWNJ‑0478:712:GW2002102894th:2:2102:8349:75792/118‑1 (MQ=60)
gtgtataagagacagCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATGTGGGC < GWNJ‑0478:712:GW2002102894th:2:2111:13395:57896/135‑1 (MQ=60)
aagagacaggtatacgcCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATGTGGGCTGCCTGGGGC < GWNJ‑0478:712:GW2002102894th:2:2205:1594:4239/133‑1 (MQ=60)
CACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1211:17694:26070/1‑78 (MQ=60)
CACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttc > GWNJ‑0478:712:GW2002102894th:2:1115:5083:78946/1‑90 (MQ=60)
GTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2203:7583:63302/1‑74 (MQ=60)
GTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2104:16248:77129/1‑74 (MQ=60)
GTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCtgtctcttatacacatctccgagcccacgagacactcg > GWNJ‑0478:712:GW2002102894th:2:2105:17081:41325/1‑112 (MQ=60)
GTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATGTGGGCTGCCTGGGGCTGGCATGGG > GWNJ‑0478:712:GW2002102894th:2:1208:10865:32134/1‑150 (MQ=60)
GTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATGTGGGCTGCCTGGGGCTGGCATGGG > GWNJ‑0478:712:GW2002102894th:2:2206:17498:29263/1‑150 (MQ=60)
TATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATGTGGGCTGCCTGGGGCTGGCATGGGG > GWNJ‑0478:712:GW2002102894th:2:1209:10079:39095/1‑150 (MQ=60)
C‑CCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATGTGGGCTGCCTGGGGCTGGCATGGGGCTGTCT > GWNJ‑0478:712:GW2002102894th:2:2212:12687:72832/1‑150 (MQ=60)
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GCCTTGTCCGCCATTTGCCTTTCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATGTGGGCTGCCTGGGGCTGGCATGGGGATGCCT > AM260479/3972035‑3972331
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| Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
