Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
582,759 |
G→T |
C352F (TGT→TTT) |
h16_A0555 → |
zinc metalloprotease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 582,759 | 0 | G | T | 100.0%
| 98.6
/ NA
| 27 | C352F (TGT→TTT) | h16_A0555 | zinc metalloprotease |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (13/14); total (13/14) |
GACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTGTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCC > AM260479/582631‑582884
|
gACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTgcgc > 2:593433/1‑134 (MQ=255)
aaaaTTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCgg > 2:474911/1‑134 (MQ=255)
aTTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc < 1:237673/134‑1 (MQ=255)
aTTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc < 1:474911/134‑1 (MQ=255)
aTTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc < 1:482480/134‑1 (MQ=255)
tCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATc > 1:953835/1‑132 (MQ=255)
aTGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGaa > 2:744162/1‑134 (MQ=255)
aCTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGcc < 1:920131/134‑1 (MQ=255)
tCACCCGCTGACCTCCGGGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATg < 2:515135/103‑1 (MQ=255)
tCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATg > 1:515135/1‑103 (MQ=255)
aCCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCa < 1:587136/132‑1 (MQ=255)
gCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGAcc > 1:818592/1‑134 (MQ=255)
ccGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGcgcg < 2:75763/85‑1 (MQ=255)
ccGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGcgcg > 1:75763/1‑85 (MQ=255)
gCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTgc > 1:368555/1‑134 (MQ=255)
gCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCgcgg < 2:953835/133‑1 (MQ=255)
aCATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTg < 2:368555/134‑1 (MQ=255)
ggACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCCGCGCTGcgcacg > 1:836068/1‑133 (MQ=255)
aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCgg > 2:425675/1‑133 (MQ=255)
cAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCAc < 1:425675/134‑1 (MQ=255)
aGGTCGCCATCACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCt > 1:738078/1‑134 (MQ=255)
cacGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTgcgc < 2:229122/121‑1 (MQ=255)
cacGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTgcgc > 1:229122/1‑121 (MQ=255)
ccTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTa < 1:744162/134‑1 (MQ=255)
ccTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTa < 2:738078/134‑1 (MQ=255)
gAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACgg > 1:511237/1‑134 (MQ=255)
aTACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGgcc < 2:511237/134‑1 (MQ=255)
|
GACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTGTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCC > AM260479/582631‑582884
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTGTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCCTTGCCTTCAC > AM260479/582627‑582894
|
tccatccaagctcgttcctttttctgtgtttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCC < GWNJ‑0478:712:GW2002102894th:2:1103:12677:46728/44‑1 (MQ=60)
GAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCC > GWNJ‑0478:712:GW2002102894th:2:2212:7970:54639/1‑148 (MQ=60)
AATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAA < GWNJ‑0478:712:GW2002102894th:2:1114:13770:92386/150‑1 (MQ=60)
AATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAA < GWNJ‑0478:712:GW2002102894th:2:1215:18004:98485/150‑1 (MQ=60)
AATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAA < GWNJ‑0478:712:GW2002102894th:2:1215:12031:59383/150‑1 (MQ=60)
tcttatttctatcttttatttgtacttttttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAA < GWNJ‑0478:712:GW2002102894th:2:1208:7226:73054/42‑1 (MQ=60)
GTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCtgtctcttatacacat > GWNJ‑0478:712:GW2002102894th:2:1216:11051:77331/1‑134 (MQ=60)
TGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCT > GWNJ‑0478:712:GW2002102894th:2:2204:18080:16524/1‑150 (MQ=60)
GACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAA < GWNJ‑0478:712:GW2002102894th:2:2211:19291:57667/150‑1 (MQ=60)
CTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTctgtctcttatacacatctccgagcccacgagacg > GWNJ‑0478:712:GW2002102894th:2:1107:15071:21360/1‑115 (MQ=60)
CACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACC < GWNJ‑0478:712:GW2002102894th:2:2104:11298:83755/148‑1 (MQ=60)
CCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCG > GWNJ‑0478:712:GW2002102894th:2:1208:15584:54917/1‑150 (MQ=60)
agcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGC < GWNJ‑0478:712:GW2002102894th:2:2105:1914:38533/86‑1 (MQ=60)
GATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCCGCGCTGCGCACGC > GWNJ‑0478:712:GW2002102894th:2:2205:11782:30599/1‑149 (MQ=60)
ATGCCAGCAAGCGCAAGGTCGCCATCACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTC > GWNJ‑0478:712:GW2002102894th:2:2115:2921:98335/1‑150 (MQ=60)
GCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGC < GWNJ‑0478:712:GW2002102894th:2:1210:8648:88827/150‑1 (MQ=60)
GCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGC > GWNJ‑0478:712:GW2002102894th:2:1114:5239:43938/1‑149 (MQ=60)
GTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGC > GWNJ‑0478:712:GW2002102894th:2:1216:20579:54509/1‑150 (MQ=60)
acagGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCCTTGCCTTCAC < GWNJ‑0478:712:GW2002102894th:2:1212:17433:58172/146‑1 (MQ=60)
GCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCCTTGCCTTCAC < GWNJ‑0478:712:GW2002102894th:2:2114:17562:32708/150‑1 (MQ=60)
CCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCCTctgtctctt > GWNJ‑0478:712:GW2002102894th:2:2106:11605:101231/1‑140 (MQ=60)
|
CGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTGTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCCTTGCCTTCAC > AM260479/582627‑582894
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
