Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
582,759 |
G→T |
C352F (TGT→TTT) |
h16_A0555 → |
zinc metalloprotease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 582,759 | 0 | G | T | 100.0%
| 95.1
/ NA
| 26 | C352F (TGT→TTT) | h16_A0555 | zinc metalloprotease |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (11/15); total (11/15) |
ATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTGTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTA > AM260479/582642‑582879
|
aTTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc < 2:453360/134‑1 (MQ=255)
aTTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc < 2:116627/134‑1 (MQ=255)
aTGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGaa > 1:80040/1‑134 (MQ=255)
cGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGGGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAAtcgtc > 2:376685/1‑134 (MQ=255)
cTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGccc < 1:708508/134‑1 (MQ=255)
aCCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc < 1:337244/107‑1 (MQ=255)
aCCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGcc > 2:337244/1‑107 (MQ=255)
aCCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGc < 2:279587/134‑1 (MQ=255)
ccGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGccc < 1:249589/119‑1 (MQ=255)
ccGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGccc > 2:249589/1‑119 (MQ=255)
gATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTgcgcg > 1:957621/1‑134 (MQ=255)
ccGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAAc < 2:661347/134‑1 (MQ=255)
cgTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGc < 2:185439/134‑1 (MQ=255)
aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTgcg > 1:593491/1‑119 (MQ=255)
aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTgcg < 2:593491/119‑1 (MQ=255)
aGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCgg > 1:315869/1‑133 (MQ=255)
gCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGccc > 1:637313/1‑77 (MQ=255)
gCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGccc < 2:637313/77‑1 (MQ=255)
gTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTcc < 1:376685/134‑1 (MQ=255)
cacGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGc < 2:80040/134‑1 (MQ=255)
ccTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTa < 2:315869/134‑1 (MQ=255)
cTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTgcgcgc > 1:145404/1‑76 (MQ=255)
cTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTgcgcgc < 2:145404/76‑1 (MQ=255)
gAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTcc > 2:772843/1‑119 (MQ=255)
gAATACGAGTTTGCGCGGATGCGGGCCCGCGGGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTcc < 1:772843/119‑1 (MQ=255)
aGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAAc > 2:259039/1‑84 (MQ=255)
|
ATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTGTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTA > AM260479/582642‑582879
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTGTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCCTTGCCTTCACCGAGCA > AM260479/582625‑582900
|
gtgtcgtttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagccagGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACCCGCCTGAATACGAGTTTGCGCGGATGCGGGC < GWNJ‑0478:712:GW2002102894th:2:1109:2221:58668/67‑1 (MQ=60)
ATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGC < GWNJ‑0478:712:GW2002102894th:2:2106:11987:97636/150‑1 (MQ=60)
gtataagagacagCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAA < GWNJ‑0478:712:GW2002102894th:2:1205:3048:41169/137‑1 (MQ=60)
ATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAAT > GWNJ‑0478:712:GW2002102894th:2:1107:4368:7821/1‑150 (MQ=60)
GCTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAAC < GWNJ‑0478:712:GW2002102894th:2:1114:10005:29241/150‑1 (MQ=60)
ACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAAC < GWNJ‑0478:712:GW2002102894th:2:2109:10278:4575/150‑1 (MQ=60)
GCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGC > GWNJ‑0478:712:GW2002102894th:2:2209:16923:38238/1‑150 (MQ=60)
ATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGC > GWNJ‑0478:712:GW2002102894th:2:2103:19668:21502/1‑150 (MQ=60)
ATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGG > GWNJ‑0478:712:GW2002102894th:2:1202:16956:2523/1‑149 (MQ=60)
gtgtctcgtgggctcggagatgtgtataagagacagACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGG < GWNJ‑0478:712:GW2002102894th:2:1114:2102:76179/114‑1 (MQ=60)
GCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGtctcttatacacatctccgagcccacgagacactcgcta > GWNJ‑0478:712:GW2002102894th:2:2105:12140:91661/1‑110 (MQ=60)
AGGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCActgtctcttatacacatctccgagcccacgagacactcgctaat > GWNJ‑0478:712:GW2002102894th:2:1109:6504:87149/1‑106 (MQ=60)
GGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGG < GWNJ‑0478:712:GW2002102894th:2:1207:18317:79905/150‑1 (MQ=60)
GGTCGCCAACACGCCTGAATACGAGTTTGCGCGGATGCGGGCCCGCGGGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGG < GWNJ‑0478:712:GW2002102894th:2:2113:17049:39678/150‑1 (MQ=60)
GAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCCTTGCCTTCACCG > GWNJ‑0478:712:GW2002102894th:2:1205:8512:25333/1‑148 (MQ=60)
GAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCCTTGCCTTCACCG > GWNJ‑0478:712:GW2002102894th:2:1215:13564:37735/1‑148 (MQ=60)
GAATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCCTTGCCTTCACCG > GWNJ‑0478:712:GW2002102894th:2:2101:16688:67694/1‑148 (MQ=60)
ATACGAGTTTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCCTctgtctcttatacac > GWNJ‑0478:712:GW2002102894th:2:2201:6604:31666/1‑135 (MQ=60)
|
ATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAGCGCAAGGTCGCCAACACGCCTGAATACGAGTGTGCGCGGATGCGGGCCCGCGTGATCCAGGAATCGTCGCCCAGCGACCTGCAGAACCTGCGCGCGGCGCTGCGCACGCAACTGCCGGGAGCAACGGCACTGCGCGCTCCGGCGCTGCACTACGGCCTTGCCTTCACCGAGCA > AM260479/582625‑582900
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
