Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,016,501 |
T→C |
V678V (GTT→GTC) |
h16_A1857 → |
Uncharacterized protein involved in exopolysaccharide biosynthesis |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,016,501 | 0 | T | C | 100.0%
| 91.0
/ NA
| 24 | V678V (GTT→GTC) | h16_A1857 | Uncharacterized protein involved in exopolysaccharide biosynthesis |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/15); total (9/15) |
GAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGC > AM260479/2016385‑2016627
|
gAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcacggca < 1:861675/134‑1 (MQ=255)
aCCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg < 1:50839/133‑1 (MQ=255)
acacTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTcac < 2:840522/134‑1 (MQ=255)
cacTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTcac > 2:493081/1‑133 (MQ=255)
ttGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGaa > 2:257285/1‑128 (MQ=255)
gCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc < 2:892535/114‑1 (MQ=255)
gCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc > 1:892535/1‑114 (MQ=255)
ggCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATcgacg > 2:33442/1‑133 (MQ=255)
aCATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACgcgc > 1:801976/1‑134 (MQ=255)
gACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAgg < 1:368098/133‑1 (MQ=255)
gACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAgg < 1:257285/133‑1 (MQ=255)
aCGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACgcgc > 1:813352/1‑119 (MQ=255)
aCGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACgcgc < 2:813352/119‑1 (MQ=255)
aCGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGcc < 2:825995/134‑1 (MQ=255)
ccGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACgg < 1:636439/132‑1 (MQ=255)
gTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcacg < 1:243641/56‑1 (MQ=255)
gTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcacg > 2:243641/1‑56 (MQ=255)
gCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGAt < 1:1016039/134‑1 (MQ=255)
tgCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACtt > 1:625263/1‑133 (MQ=255)
tGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTc < 2:262133/131‑1 (MQ=255)
tGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTc > 1:262133/1‑131 (MQ=255)
cGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCa < 2:625263/134‑1 (MQ=255)
cATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCAcc < 1:493081/134‑1 (MQ=255)
gCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGc < 1:84289/134‑1 (MQ=255)
|
GAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGC > AM260479/2016385‑2016627
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGGGCCAAGCGAGCTGTTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTACAGCTATGCCTACGGTGCC > AM260479/2016357‑2016648
|
CGGGCCAAGCGAGCTGTTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCAT < GWNJ‑0478:712:GW2002102894th:2:2111:7851:86339/150‑1 (MQ=60)
GGGCCAAGCGAGCTGTTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATC < GWNJ‑0478:712:GW2002102894th:2:2102:5196:23279/150‑1 (MQ=60)
cggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCAC < GWNJ‑0478:712:GW2002102894th:2:1212:7934:16044/95‑1 (MQ=60)
GCGAGCTGTTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCAC < GWNJ‑0478:712:GW2002102894th:2:2210:11797:38994/150‑1 (MQ=60)
agcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCAC < GWNJ‑0478:712:GW2002102894th:2:1116:3191:26711/86‑1 (MQ=60)
AGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGT < GWNJ‑0478:712:GW2002102894th:2:2209:11284:91401/150‑1 (MQ=60)
GACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAAC < GWNJ‑0478:712:GW2002102894th:2:1102:18277:51810/149‑1 (MQ=60)
CTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGctgtct > GWNJ‑0478:712:GW2002102894th:2:2207:16287:86564/1‑144 (MQ=60)
TTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCC > GWNJ‑0478:712:GW2002102894th:2:2203:16771:99948/1‑150 (MQ=60)
CAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttc > GWNJ‑0478:712:GW2002102894th:2:1210:4448:70344/1‑90 (MQ=60)
CAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttc > GWNJ‑0478:712:GW2002102894th:2:2103:16892:68797/1‑90 (MQ=60)
ACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCC > GWNJ‑0478:712:GW2002102894th:2:2202:19439:79291/1‑150 (MQ=60)
TGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTAC < GWNJ‑0478:712:GW2002102894th:2:1208:1877:52348/149‑1 (MQ=60)
TGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTAC < GWNJ‑0478:712:GW2002102894th:2:1201:18697:14777/149‑1 (MQ=60)
GCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAAC < GWNJ‑0478:712:GW2002102894th:2:2109:12775:5045/148‑1 (MQ=60)
TGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTC > GWNJ‑0478:712:GW2002102894th:2:2108:4373:34119/1‑149 (MQ=60)
GGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCAC < GWNJ‑0478:712:GW2002102894th:2:2215:11394:99556/150‑1 (MQ=60)
CAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCG > GWNJ‑0478:712:GW2002102894th:2:1201:2288:48582/1‑150 (MQ=60)
GCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTACAGCT < GWNJ‑0478:712:GW2002102894th:2:1214:6072:55090/150‑1 (MQ=60)
CATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaacgcgcatcgccgat > GWNJ‑0478:712:GW2002102894th:2:1204:18766:78514/1‑55 (MQ=60)
GGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaacaaaggaaggtcagaaaacacatataaa > GWNJ‑0478:712:GW2002102894th:2:1214:1601:42974/1‑42 (MQ=60)
GGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTACAGCTATGCCTAC < GWNJ‑0478:712:GW2002102894th:2:1107:3835:76137/150‑1 (MQ=60)
GTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaaacaaaaagaagagattgagataaccac > GWNJ‑0478:712:GW2002102894th:2:2209:16985:68041/1‑40 (MQ=60)
GTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttc > GWNJ‑0478:712:GW2002102894th:2:2215:19831:42833/1‑90 (MQ=60)
|
CGGGCCAAGCGAGCTGTTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTACAGCTATGCCTACGGTGCC > AM260479/2016357‑2016648
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
