Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
2,018,102 |
+C |
intergenic (+111/+98) |
h16_B1778 → / ← h16_B1779 |
putative peptidase/putative peptidase, S33 family |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 2,018,101 | 1 | . | C | 100.0%
| 79.1
/ NA
| 21 | intergenic (+110/+99) | h16_B1778/h16_B1779 | putative peptidase/putative peptidase, S33 family |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (10/11); total (10/11) |
CAGCGTTCAGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGA‑CATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCTACATCGAGCA > AM260480/2017969‑2018222
|
cAGCGTTCAGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGA‑c > 1:1050264/1‑134 (MQ=255)
cAGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATgcg > 1:248312/1‑133 (MQ=255)
aGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATgcgc < 1:282837/133‑1 (MQ=255)
aGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATgcgc < 2:248312/133‑1 (MQ=255)
cgcTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGcc < 1:187798/134‑1 (MQ=255)
cgcgGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGcgccg > 2:60490/1‑134 (MQ=255)
cgggcgCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAgcg < 1:60490/134‑1 (MQ=255)
gggcgCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAgcgc > 1:777568/1‑134 (MQ=255)
tGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAg > 1:1084111/1‑134 (MQ=255)
cGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAgcgc > 2:900138/1‑80 (MQ=255)
cGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAgcgc < 1:900138/80‑1 (MQ=255)
cGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCg > 1:735733/1‑134 (MQ=255)
cGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCg > 1:272487/1‑134 (MQ=255)
cTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTc < 2:1050264/134‑1 (MQ=255)
cTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTc < 2:735733/134‑1 (MQ=255)
cTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTc < 2:742088/134‑1 (MQ=255)
tCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTTCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAgcg > 2:813989/1‑134 (MQ=255)
gggCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTTCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCt < 1:813989/134‑1 (MQ=255)
gcATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCt < 2:272487/134‑1 (MQ=255)
cGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTc < 1:806045/115‑1 (MQ=255)
cGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTc > 2:806045/1‑115 (MQ=255)
gggCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCTACATCGAGCa > 2:321855/1‑134 (MQ=255)
|
CAGCGTTCAGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGA‑CATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCTACATCGAGCA > AM260480/2017969‑2018222
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCTCAAGAGTTGCGCGCAGCGTTCAGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGA‑CATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCTACATCGAGCACCGCCTGCGCA > AM260480/2017953‑2018233
|
GCTCAAGAGTTGCGCGCAGCGTTCAGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGA‑C < GWNJ‑0478:712:GW2002102894th:2:1214:5348:75366/150‑1 (MQ=60)
CTCAAGAGTTGCGCGCAGCGTTCAGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGA‑CC > GWNJ‑0478:712:GW2002102894th:2:2210:18967:47369/1‑150 (MQ=60)
CTCAAGAGTTGCGCGCAGCGTTCAGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGA‑CC > GWNJ‑0478:712:GW2002102894th:2:1201:13115:76930/1‑150 (MQ=60)
GTTGCGCGCAGCGTTCAGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGC > GWNJ‑0478:712:GW2002102894th:2:1114:17591:22364/1‑149 (MQ=60)
CAGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGC < GWNJ‑0478:712:GW2002102894th:2:1116:4034:93388/149‑1 (MQ=60)
GCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTC < GWNJ‑0478:712:GW2002102894th:2:1113:3996:15529/150‑1 (MQ=60)
aagcaagggcggctCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCT > GWNJ‑0478:712:GW2002102894th:2:2113:13919:62233/15‑150 (MQ=60)
GGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGC > GWNJ‑0478:712:GW2002102894th:2:2213:20710:19311/1‑150 (MQ=60)
GCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGG < GWNJ‑0478:712:GW2002102894th:2:1102:8744:87374/150‑1 (MQ=60)
gcgagtgtctcgtgggctcggagatgtgtataagagacaGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGC < GWNJ‑0478:712:GW2002102894th:2:2203:19110:92109/111‑1 (MQ=60)
caagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAG < GWNJ‑0478:712:GW2002102894th:2:2209:2948:83668/85‑1 (MQ=60)
gttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGC < GWNJ‑0478:712:GW2002102894th:2:1211:13584:35109/72‑1 (MQ=60)
CCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGT > GWNJ‑0478:712:GW2002102894th:2:1116:8990:41089/1‑150 (MQ=60)
CCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGT > GWNJ‑0478:712:GW2002102894th:2:2111:10691:49084/1‑150 (MQ=60)
gacagTCCGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCTACAT < GWNJ‑0478:712:GW2002102894th:2:2115:15204:5299/145‑1 (MQ=60)
atgtgtataagagacaGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCTACAT < GWNJ‑0478:712:GW2002102894th:2:1115:19145:50874/134‑1 (MQ=60)
CGGGCGCATGGTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTTCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCTACATCGAGCAC < GWNJ‑0478:712:GW2002102894th:2:2115:10749:41325/150‑1 (MQ=60)
GTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCTcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:1111:1954:95212/1‑129 (MQ=60)
GTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCTACATCtgtctcttatacacat > GWNJ‑0478:712:GW2002102894th:2:2201:9977:74520/1‑134 (MQ=60)
GTCCGGGGCATGCCCGACCATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCTACATCtgtctcttatacacat > GWNJ‑0478:712:GW2002102894th:2:1107:11496:82078/1‑134 (MQ=60)
|
GCTCAAGAGTTGCGCGCAGCGTTCAGGTCGTTGCGCTGAGCGCGGCAGGCGGGCGGCGGCGGGCGCTGAAGCATGGCAATGCCTTCAGCGCCCGAGCCGCCCTTGCTTCGTGCCCGCTTATCCGGGCGCATGGTCCGGGGCATGCCCGA‑CATGCGCACCGATGGCCAGCGCCGCCCAGCTCCGATGCCAGCGCTGACAGCGTCATGGCAGCCAGTATCAGCAATCTGCGCATTGCGCCCCTCATGCGTCAGCGCCTGTCTACATCGAGCACCGCCTGCGCA > AM260480/2017953‑2018233
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
