Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
951,073 |
+G |
intergenic (‑29/+230) |
h16_B0838 ← / ← h16_B0839 |
conserved hypothetical protein/transcriptional regulator, CRP‑family |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 951,073 | 1 | . | G | 100.0%
| 74.3
/ NA
| 20 | intergenic (‑29/+230) | h16_B0838/h16_B0839 | conserved hypothetical protein/transcriptional regulator, CRP‑family |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (10/10); total (10/10) |
CGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCG > AM260480/950964‑951197
|
cGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGAcg > 2:383064/1‑134 (MQ=255)
gggCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCgccagcca > 2:913120/1‑134 (MQ=255)
ggCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGAc > 1:7640/1‑132 (MQ=255)
cACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGAttt < 1:978142/132‑1 (MQ=255)
cACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGAttt < 1:73905/132‑1 (MQ=255)
aCCGGCTGGCTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCgcca < 2:233264/114‑1 (MQ=255)
aCCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCgcca > 1:233264/1‑114 (MQ=255)
catcggctgcatcggctgCGTCATCGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTc < 2:7640/134‑1 (MQ=255)
tCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGcc > 1:461992/1‑133 (MQ=255)
tGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGAttt < 1:410280/90‑1 (MQ=255)
tGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGAttt > 2:410280/1‑90 (MQ=255)
gCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAgg < 1:383064/133‑1 (MQ=255)
gctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAgg < 2:361587/132‑1 (MQ=255)
gctggctCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAgg > 1:361587/1‑132 (MQ=255)
aGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAgc < 1:913120/134‑1 (MQ=255)
ggggCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTa > 1:204394/1‑99 (MQ=255)
ggggCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTa < 2:204394/99‑1 (MQ=255)
gCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCCCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGcgccg > 2:326048/1‑134 (MQ=255)
tGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCt < 1:830020/76‑1 (MQ=255)
tGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCt > 2:830020/1‑76 (MQ=255)
|
CGACGGCATGGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCG > AM260480/950964‑951197
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCGCAGCACCTGAC > AM260480/950973‑951208
|
GGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACC > GWNJ‑0478:712:GW2002102894th:2:1101:9441:57362/1‑148 (MQ=60)
GGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATctgtct > GWNJ‑0478:712:GW2002102894th:2:1114:12863:66960/1‑144 (MQ=60)
cgtgggctcggagatgtgtataagagacaGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTT < GWNJ‑0478:712:GW2002102894th:2:1211:17583:99635/121‑1 (MQ=60)
GCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTT < GWNJ‑0478:712:GW2002102894th:2:1107:4767:9959/148‑1 (MQ=60)
GCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTT < GWNJ‑0478:712:GW2002102894th:2:2213:13105:89846/148‑1 (MQ=60)
atgtagctccgtctcgtgggctcggagatgtgtataagagacaGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTC < GWNJ‑0478:712:GW2002102894th:2:2204:3115:90679/107‑1 (MQ=60)
GCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCT > GWNJ‑0478:712:GW2002102894th:2:1212:4513:92408/1‑149 (MQ=60)
GCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGA > GWNJ‑0478:712:GW2002102894th:2:1208:5877:11665/1‑149 (MQ=60)
GGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCAC < GWNJ‑0478:712:GW2002102894th:2:1209:11414:44231/149‑1 (MQ=60)
GCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCActgtctcttatacacatctcc > GWNJ‑0478:712:GW2002102894th:2:1115:3281:4253/1‑129 (MQ=60)
CAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCGCAGCACCTGAC < GWNJ‑0478:712:GW2002102894th:2:2211:5791:15531/150‑1 (MQ=60)
|
GGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCGCAGCACCTGAC > AM260480/950973‑951208
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
