Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
951,073 |
+G |
intergenic (‑29/+230) |
h16_B0838 ← / ← h16_B0839 |
conserved hypothetical protein/transcriptional regulator, CRP‑family |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 951,073 | 1 | . | G | 100.0%
| 48.4
/ NA
| 14 | intergenic (‑29/+230) | h16_B0838/h16_B0839 | conserved hypothetical protein/transcriptional regulator, CRP‑family |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (8/6); total (8/6) |
GGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCG > AM260480/950982‑951197
|
gggCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCgccagcca > 2:372342/1‑134 (MQ=255)
ggCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCctgctg < 2:480915/90‑5 (MQ=255)
ggCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCctgctg > 1:480915/1‑86 (MQ=255)
ggCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCctgc > 1:170345/1‑86 (MQ=255)
ggCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCctgc < 2:170345/88‑3 (MQ=255)
aCCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCgg > 1:867995/1‑92 (MQ=255)
aCCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCgg < 2:867995/92‑1 (MQ=255)
gCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGc > 1:44016/1‑130 (MQ=255)
aCTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGAttt < 1:789429/123‑1 (MQ=255)
aCTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGAttt > 2:789429/1‑123 (MQ=255)
catcggctgcatcggctgCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCAtt < 2:44016/133‑1 (MQ=255)
gctgcatcggctgcGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTa > 1:487162/1‑134 (MQ=255)
atcggctgcGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAcctcc < 2:487162/134‑1 (MQ=255)
tCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGcc > 2:619642/1‑133 (MQ=255)
tCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAg > 1:1098768/1‑134 (MQ=255)
gCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACa > 1:995462/1‑116 (MQ=255)
gCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACa < 2:995462/116‑1 (MQ=255)
gCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGcgccg < 1:372342/134‑1 (MQ=255)
|
GGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCG > AM260480/950982‑951197
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCGCAGCACCTGACATTC > AM260480/950973‑951212
|
GGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGctgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:1110:13350:19297/1‑118 (MQ=60)
GGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTActgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:1213:4075:53272/1‑120 (MQ=60)
GGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCtgtctcttatacacatctccgagcccacgagacactcgctaatc > GWNJ‑0478:712:GW2002102894th:2:2212:20242:10307/1‑106 (MQ=60)
GGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCtgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:2116:16837:92002/1‑123 (MQ=60)
GGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCC > GWNJ‑0478:712:GW2002102894th:2:1105:9016:79420/1‑146 (MQ=60)
GGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgcc > GWNJ‑0478:712:GW2002102894th:2:2201:9797:24297/1‑97 (MQ=60)
GCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTT < GWNJ‑0478:712:GW2002102894th:2:2112:13337:22663/148‑1 (MQ=60)
GACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTAC > GWNJ‑0478:712:GW2002102894th:2:1213:12542:83947/1‑150 (MQ=60)
GCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAActgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2209:17649:97491/1‑69 (MQ=60)
GCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGG > GWNJ‑0478:712:GW2002102894th:2:2213:19200:88977/1‑150 (MQ=60)
cggagatgtgtataagagacagGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAAC < GWNJ‑0478:712:GW2002102894th:2:1113:7051:100785/128‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacaGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGA < GWNJ‑0478:712:GW2002102894th:2:2113:14760:7331/118‑1 (MQ=60)
GCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTC‑CTGT > GWNJ‑0478:712:GW2002102894th:2:2207:7756:51813/1‑150 (MQ=60)
GGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGActgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:1108:18614:45946/1‑117 (MQ=60)
GGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCctgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:2208:16869:2659/1‑123 (MQ=60)
GGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCGCAGCACCTGACATTC > GWNJ‑0478:712:GW2002102894th:2:2209:10131:40164/1‑150 (MQ=60)
GGCTGAGCCTGCTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCGCAGCACCTGACATTC < GWNJ‑0478:712:GW2002102894th:2:1207:3724:54681/150‑1 (MQ=60)
|
GGCTTGCACGGGCTGGGCGGACTGCACCGGCTGACTGGATTGCATCGGCTGCATCGGCTGCGTCATTGGCATCGGCTGGCTCGGCTGCAGGGGCTGAGCCT‑CTGCGCAAACAGCGGGTAGGACGCGATCATCGCGCCAGCCACGACCAGGGATTTCGCCAGATGTTGCTTCATTCGTGTACCTCCTAGAACGCCTACAGGCAAGGGAACAGTCACTGTAGCGCCGCAGCACCTGACATTC > AM260480/950973‑951212
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
