Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
2,308,233 |
(C)5→4 |
intergenic (+176/‑161) |
h16_B2050 → / → h16_B2051 |
response regulator containing a GGDEF effector domain/conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 2,308,229 | 0 | C | . | 100.0%
| 139.8
/ NA
| 31 | intergenic (+172/‑165) | h16_B2050/h16_B2051 | response regulator containing a GGDEF effector domain/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (11/20); total (11/20) |
GGCCACCGGCCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAGCCCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAACTGGCCACGCGACCGGCGCGCCCC > AM260480/2308101‑2308339
|
ggcCACCGGCCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAGccccgc > 1:570002/1‑132 (MQ=255)
ccACCGGCCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCTCCGCTTGTCGGAATTGTCCGACATGCGATTCGGAGccccgcgc > 1:897808/1‑130 (MQ=255)
ccACCGGCCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAGccccgcgc > 2:15656/1‑130 (MQ=255)
ccACCGGCCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAGccccgcgc > 1:947691/1‑130 (MQ=255)
ccAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGtctc < 2:372625/134‑1 (MQ=255)
cAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCg < 2:496127/134‑1 (MQ=255)
cAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCg < 1:509258/134‑1 (MQ=255)
cAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCg < 1:772277/134‑1 (MQ=255)
cGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGcccc < 2:570002/134‑1 (MQ=255)
caGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGa < 1:480988/134‑1 (MQ=255)
gCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCAc < 2:947691/134‑1 (MQ=255)
cGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGc < 2:753062/132‑1 (MQ=255)
cGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTg < 2:441551/134‑1 (MQ=255)
ggCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCg < 2:421294/85‑1 (MQ=255)
ggCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCg > 1:421294/1‑85 (MQ=255)
ccggccCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTa < 1:312845/96‑1 (MQ=255)
ccggccCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTa > 2:312845/1‑96 (MQ=255)
gcacgcacgcGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTa > 1:535783/1‑83 (MQ=255)
gcacgcacgcGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTa < 2:535783/83‑1 (MQ=255)
gTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTa > 2:888277/1‑73 (MQ=255)
gTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTa < 1:888277/73‑1 (MQ=255)
ccGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTa < 1:37051/69‑1 (MQ=255)
ccGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTa > 2:37051/1‑69 (MQ=255)
gTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAACTGGCCACGCGACCGgcg < 1:15656/133‑1 (MQ=255)
cGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTa > 1:486199/1‑61 (MQ=255)
cGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTa < 2:486199/61‑1 (MQ=255)
cGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAACTGGCCACGCGACCGgcgcgc > 2:38437/1‑134 (MQ=255)
gAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAg > 1:1003201/1‑76 (MQ=255)
gAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAg < 2:1003201/76‑1 (MQ=255)
gAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAACTGGCCACGCGACCGGCGCGccc > 2:729988/1‑134 (MQ=255)
aaTTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAACTGGCCACGCGACCGGCGCGcccc < 2:222992/134‑1 (MQ=255)
aTTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAAc < 2:848200/110‑1 (MQ=255)
aTTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAAc > 1:848200/1‑110 (MQ=255)
tCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTg < 1:225516/88‑1 (MQ=255)
tCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTg > 2:225516/1‑88 (MQ=255)
|
GGCCACCGGCCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAGCCCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAACTGGCCACGCGACCGGCGCGCCCC > AM260480/2308101‑2308339
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CAGTTACACACTGGCGGCCACCGGCCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAGCCCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAACTGGCCACGCGACCGGCGCGCCCCCGCGCGGGCATGGGCCAGATCGTCTCTGACTCCTCCAAC > AM260480/2308086‑2308378
|
CAGTTACACACTGGCGGCCACCGGCCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCG > GWNJ‑0478:712:GW2002102894th:2:2105:7014:66120/1‑150 (MQ=60)
GTTACACACTGGCGGCCACCGGCCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCTCCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCC > GWNJ‑0478:712:GW2002102894th:2:2208:19246:19968/1‑150 (MQ=60)
GTTACACACTGGCGGCCACCGGCCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCC > GWNJ‑0478:712:GW2002102894th:2:2212:14262:19426/1‑150 (MQ=60)
CCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATAC < GWNJ‑0478:712:GW2002102894th:2:1216:20948:43888/150‑1 (MQ=60)
CCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATAC < GWNJ‑0478:712:GW2002102894th:2:2116:18899:95404/150‑1 (MQ=60)
CCAGCCCGGCCAACGCCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCAT < GWNJ‑0478:712:GW2002102894th:2:1215:18697:90911/150‑1 (MQ=60)
gcacagccgcaacaaccagttacacactggcggccaccggccaacgtCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCAC > GWNJ‑0478:712:GW2002102894th:2:2106:18230:94582/48‑150 (MQ=60)
gagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGAC < GWNJ‑0478:712:GW2002102894th:2:2207:18302:60417/103‑1 (MQ=60)
atacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGAC < GWNJ‑0478:712:GW2002102894th:2:1105:6372:57644/99‑1 (MQ=60)
cggagatgtgtataagagacaGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGAC < GWNJ‑0478:712:GW2002102894th:2:1205:16393:97035/127‑1 (MQ=60)
GGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTgtctcttatacacatctccgagcccacgagacg > GWNJ‑0478:712:GW2002102894th:2:1210:10007:63381/1‑117 (MQ=60)
cgtgggctcggagatgtgtataagagacagCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCC < GWNJ‑0478:712:GW2002102894th:2:1114:13415:23684/118‑1 (MQ=60)
GGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACctgtctcttatacacatctccgagcccacgagacgg > GWNJ‑0478:712:GW2002102894th:2:2103:9221:22221/1‑113 (MQ=60)
CGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtctt > GWNJ‑0478:712:GW2002102894th:2:1214:9204:18853/1‑91 (MQ=60)
CGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGtctcttatacacatctccgagcccacgagacggagctacat > GWNJ‑0478:712:GW2002102894th:2:2215:16112:27742/1‑109 (MQ=60)
TCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAACTGGCCACGCGACCGGCtgtctctt > GWNJ‑0478:712:GW2002102894th:2:2209:20908:9360/1‑141 (MQ=60)
TGTCGGAATTGTCCGACATGCGATTCGGAG‑CCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAACTGGCCACGCGACCGGCGCGCCCCCGCGCGGGC < GWNJ‑0478:712:GW2002102894th:2:1103:7243:14482/149‑1 (MQ=60)
GCCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAACTGGCCACGCGACCGGCGCGCCCCCGCGCGGGCATGGGCCAGATCGTCTCTGACTCCTCCAAC > GWNJ‑0478:712:GW2002102894th:2:2105:13243:99472/1‑150 (MQ=60)
|
CAGTTACACACTGGCGGCCACCGGCCAACCGGGCCACCAGCCCGGCCAACGTCCCGCCAGGCCCCGCACCTGCGGGCCTGGCCGGCCCCGCCACGCACGCACGCGTCACCGCTTGTCGGAATTGTCCGACATGCGATTCGGAGCCCCCGCGCCTGTCTCGCCCCTGGGTTGATACCTATCGTTGCATCACGACAGGTGCAATCGCTGCGCCTGCAACAACAGACAGCCAACTGGCCACGCGACCGGCGCGCCCCCGCGCGGGCATGGGCCAGATCGTCTCTGACTCCTCCAAC > AM260480/2308086‑2308378
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
