Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
2,431,093 |
Δ1 bp |
coding (2380/2469 nt) |
h16_B2148 → |
membrane carboxypeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 2,431,092 | 0 | T | . | 100.0%
| 38.8
/ NA
| 10 | coding (2379/2469 nt) | h16_B2148 | membrane carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (4/6); total (4/6) |
CCCGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGATTCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCC > AM260480/2430963‑2431168
|
cccGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGAtcggg < 2:1025819/134‑5 (MQ=255)
cccGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGAtcggg < 2:339024/134‑5 (MQ=255)
cGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCggggg > 1:598920/1‑134 (MQ=255)
ggCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCggggg > 2:622386/1‑133 (MQ=255)
ccgcgccCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGAc > 2:376519/1‑134 (MQ=255)
ccGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGAt < 1:376519/134‑1 (MQ=255)
gcgTCGGCCGGCGCTTCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGc < 2:987796/134‑1 (MQ=255)
gcgTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGc < 1:784039/134‑1 (MQ=255)
ggccggcGCTGCCCACGCCGGAGGCCGAGGTCCCGCCCCCGGCCGGCGCGGCATCGCCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCGGGCGCTGGA‑TCGGGGGGGGACGGTGATTGCGGGGCCGCAg > 1:987796/1‑134 (MQ=255)
ccgccgGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCAcc < 2:88127/134‑1 (MQ=255)
ccggcGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCATCAg < 1:622386/134‑1 (MQ=255)
gcgcGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGcc < 2:598920/134‑1 (MQ=255)
|
CCCGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGATTCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCC > AM260480/2430963‑2431168
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AGGCCAGCGAGACCCCGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGATTCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAACGCCGCCCCTGCCTCCCCGGCTTCCGCAAATCCGCCCGCACCGCCTGCGCCCC > AM260480/2430950‑2431235
|
AGGCCAGCGAGACCCCGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGG > GWNJ‑0478:712:GW2002102894th:2:2107:7367:61324/1‑150 (MQ=60)
GCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCCCGCCCCCGGCCGGCGCGGCATCGCCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCGGGCGCTGGA‑TCGGGGGGGGACGGTGATTGCGGGGCCGCAGG > GWNJ‑0478:712:GW2002102894th:2:2214:11859:43406/1‑150 (MQ=60)
CCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGA < GWNJ‑0478:712:GW2002102894th:2:1209:5347:3217/150‑1 (MQ=60)
tcaggctcagtatgtctagtttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACA < GWNJ‑0478:712:GW2002102894th:2:1210:9352:31702/53‑1 (MQ=60)
CGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCG < GWNJ‑0478:712:GW2002102894th:2:2201:6498:78277/150‑1 (MQ=60)
tttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGC < GWNJ‑0478:712:GW2002102894th:2:1215:7331:8951/80‑1 (MQ=60)
GCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCATCAGGCCGAACCAGCGCCT < GWNJ‑0478:712:GW2002102894th:2:2108:11139:15620/150‑1 (MQ=60)
gggctcggagatgtgtataagagacagGTCCAGCCTGGCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAACGCCGCCCCTGCCTC < GWNJ‑0478:712:GW2002102894th:2:2204:2023:83927/123‑1 (MQ=60)
AGTCCAGCCTGGCGCTGGA‑TCGGTGGGAGACGGTGATTGCGGTGCCGCAGGACActgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaaagtcggaacacaatcatt > GWNJ‑0478:712:GW2002102894th:2:2215:14692:28283/1‑54 (MQ=60)
GCGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACActgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaataaaaaaggacagaccagctccagaccat > GWNJ‑0478:712:GW2002102894th:2:1103:14762:28170/1‑43 (MQ=60)
CGCTGGA‑TCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtc > GWNJ‑0478:712:GW2002102894th:2:2210:5845:12998/1‑95 (MQ=60)
|
AGGCCAGCGAGACCCCGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCGGCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGATTCGGGGGGAGACGGTGATTGCGGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAACGCCGCCCCTGCCTCCCCGGCTTCCGCAAATCCGCCCGCACCGCCTGCGCCCC > AM260480/2430950‑2431235
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
