Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
208,953 |
A→C |
V315V (GTT→GTG) |
h16_A0195 ← |
Putative GTPase (G3E family) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 208,953 | 0 | A | C | 100.0%
| 98.1
/ NA
| 26 | V315V (GTT→GTG) | h16_A0195 | Putative GTPase (G3E family) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (14/12); total (14/12) |
AGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGA > AM260479/208825‑209078
|
aGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc < 1:98518/134‑1 (MQ=255)
gcgGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCacac > 2:200820/1‑122 (MQ=255)
gcgGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCacac < 1:200820/122‑1 (MQ=255)
tCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCtt < 1:1061184/134‑1 (MQ=255)
gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc > 1:1000975/1‑105 (MQ=255)
gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc < 2:1000975/105‑1 (MQ=255)
ctctTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGa > 2:82709/1‑134 (MQ=255)
ctccCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGcagcag > 2:819824/1‑105 (MQ=255)
ctccCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGcagcag < 1:819824/105‑1 (MQ=255)
aCTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc < 1:63407/67‑1 (MQ=255)
aCTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc > 2:63407/1‑67 (MQ=255)
aCTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatatat < 1:248116/77‑1 (MQ=255)
aCTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatatat > 2:248116/1‑77 (MQ=255)
aCTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt < 1:1016062/103‑1 (MQ=255)
aCTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt > 2:1016062/1‑103 (MQ=255)
ccGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCac < 1:385744/56‑1 (MQ=255)
ccGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCac > 2:385744/1‑56 (MQ=255)
cTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTa < 1:1034512/126‑1 (MQ=255)
cTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTa > 2:1034512/1‑126 (MQ=255)
gACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatat < 2:671221/41‑1 (MQ=255)
gACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatat > 1:671221/1‑41 (MQ=255)
ggAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCg > 1:445616/1‑133 (MQ=255)
ggAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCg > 2:470543/1‑133 (MQ=255)
ttGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt > 2:62041/1‑50 (MQ=255)
ttGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt < 1:62041/50‑1 (MQ=255)
ttGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGacgaacga > 2:268292/1‑134 (MQ=255)
|
AGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGA > AM260479/208825‑209078
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AGGCAGTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGACGCAATGCGGTCGGTATGGTGG > AM260479/208804‑209100
|
gattagcgagtgtctcgtgggctcggagatgtgtataagagacagACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCC < GWNJ‑0478:712:GW2002102894th:2:1101:16657:10713/105‑1 (MQ=60)
GGCAGTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCC > GWNJ‑0478:712:GW2002102894th:2:1108:14599:51685/1‑149 (MQ=60)
GCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATA < GWNJ‑0478:712:GW2002102894th:2:2104:19018:61689/150‑1 (MQ=60)
CCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATAT < GWNJ‑0478:712:GW2002102894th:2:1106:6239:74014/150‑1 (MQ=60)
gagagcaggtgctatctgggtgattttggttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagcccTGGAACACCACTTTGCGGTCCACACCGTCCATATATAG < GWNJ‑0478:712:GW2002102894th:2:1207:18133:28906/38‑1 (MQ=60)
agcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAG < GWNJ‑0478:712:GW2002102894th:2:1206:7835:33969/86‑1 (MQ=60)
ggcatacgagattagcgagtgtctcgtgggctcgggatgtgtataagagacaGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTAC < GWNJ‑0478:712:GW2002102894th:2:1104:5837:6678/98‑1 (MQ=60)
GAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATctgtc > GWNJ‑0478:712:GW2002102894th:2:1213:5588:66224/1‑145 (MQ=60)
GAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTAC < GWNJ‑0478:712:GW2002102894th:2:1106:5761:16018/150‑1 (MQ=60)
GAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTA < GWNJ‑0478:712:GW2002102894th:2:1113:1779:81421/149‑1 (MQ=60)
tagcgagtgtctcgtgggctcggagatgtgtataagagacaGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAG < GWNJ‑0478:712:GW2002102894th:2:1114:16914:21426/108‑1 (MQ=60)
GGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACctgtctcttatacac > GWNJ‑0478:712:GW2002102894th:2:2207:17793:83188/1‑135 (MQ=60)
GTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTT < GWNJ‑0478:712:GW2002102894th:2:2212:11227:5309/150‑1 (MQ=60)
GATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATctgtctcttatacacatctccgagcccacgagac > GWNJ‑0478:712:GW2002102894th:2:1210:7356:56119/1‑115 (MQ=60)
gtgtataagagacagGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATAC < GWNJ‑0478:712:GW2002102894th:2:2115:2441:68155/135‑1 (MQ=60)
atgtgtataagagacaGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGC < GWNJ‑0478:712:GW2002102894th:2:2208:8141:67037/134‑1 (MQ=60)
tttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagcccTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGC < GWNJ‑0478:712:GW2002102894th:2:1102:2724:97318/77‑1 (MQ=60)
CCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGctgtctcttatacacatctccgagcccacgagacactcgct > GWNJ‑0478:712:GW2002102894th:2:1203:10979:66979/1‑109 (MQ=60)
GGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttga > GWNJ‑0478:712:GW2002102894th:2:2103:3265:18857/1‑82 (MQ=60)
GCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2107:13819:89289/1‑71 (MQ=60)
GCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTC < GWNJ‑0478:712:GW2002102894th:2:2211:9682:66525/150‑1 (MQ=60)
GCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGG > GWNJ‑0478:712:GW2002102894th:2:1211:8085:70634/1‑149 (MQ=60)
CCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaacaagagcacggagaaaaaccgcacaggcaccgcg > GWNJ‑0478:712:GW2002102894th:2:1105:1618:95155/1‑39 (MQ=60)
CCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaggaaagatagaaacagcggtgatagagtagc > GWNJ‑0478:712:GW2002102894th:2:1210:7203:17688/1‑39 (MQ=60)
CCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaaaaagaaagaagagcacgcagaggc > GWNJ‑0478:712:GW2002102894th:2:2109:3858:14259/1‑44 (MQ=60)
CCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaaaaaaaaaaatatcgaatcaaa > GWNJ‑0478:712:GW2002102894th:2:2203:10250:17615/1‑45 (MQ=60)
GGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGACGCAATGCGGTCGGTATGGT < GWNJ‑0478:712:GW2002102894th:2:1116:5254:21050/150‑1 (MQ=60)
GTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGACGCAATGCGGTCGGTATGGTC > GWNJ‑0478:712:GW2002102894th:2:1110:14813:62111/1‑150 (MQ=60)
CCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGACGCAATGCGGTCGGTATGGTGG < GWNJ‑0478:712:GW2002102894th:2:1213:12896:2126/149‑1 (MQ=60)
|
AGGCAGTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGACGCAATGCGGTCGGTATGGTGG > AM260479/208804‑209100
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
