Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
208,953 |
A→C |
V315V (GTT→GTG) |
h16_A0195 ← |
Putative GTPase (G3E family) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 208,953 | 0 | A | C | 100.0%
| 77.1
/ NA
| 21 | V315V (GTT→GTG) | h16_A0195 | Putative GTPase (G3E family) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (11/10); total (11/10) |
GGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGA > AM260479/208826‑209078
|
ggATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCg > 1:887257/1‑134 (MQ=255)
gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc < 1:749090/132‑1 (MQ=255)
gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc > 2:749090/1‑132 (MQ=255)
gcgGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatatat < 1:523914/133‑1 (MQ=255)
gcgGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatatat < 2:245750/133‑1 (MQ=255)
ggTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGccc > 1:819251/1‑134 (MQ=255)
acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGt > 2:915049/1‑134 (MQ=255)
cgcgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc < 1:476007/92‑1 (MQ=255)
cgcgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc > 2:476007/1‑92 (MQ=255)
cgcgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTccc < 1:915049/133‑1 (MQ=255)
cccGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGtata < 1:467968/133‑1 (MQ=255)
gATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc < 1:215041/60‑1 (MQ=255)
gATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc > 2:215041/1‑60 (MQ=255)
aGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGtata < 1:678404/88‑1 (MQ=255)
aGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGtata > 2:678404/1‑88 (MQ=255)
ggAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCg > 1:368017/1‑133 (MQ=255)
caccacTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGc < 2:695235/80‑1 (MQ=255)
caccacTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGc > 1:695235/1‑80 (MQ=255)
ttGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAg < 1:935898/68‑1 (MQ=255)
ttGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAg > 2:935898/1‑68 (MQ=255)
ttGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGacgaacga > 2:354040/1‑134 (MQ=255)
|
GGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGA > AM260479/208826‑209078
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGACGCAATGCGGTCGGTATG > AM260479/208809‑209096
|
ttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAC < GWNJ‑0478:712:GW2002102894th:2:2207:17308:56681/75‑1 (MQ=60)
TCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGT > GWNJ‑0478:712:GW2002102894th:2:2207:2795:54079/1‑150 (MQ=60)
CCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATAT < GWNJ‑0478:712:GW2002102894th:2:1105:19537:13269/150‑1 (MQ=60)
CCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATAT < GWNJ‑0478:712:GW2002102894th:2:1208:11530:24668/150‑1 (MQ=60)
ttctttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATAT < GWNJ‑0478:712:GW2002102894th:2:2213:12284:61068/70‑1 (MQ=60)
gtagctccgtctcgtgggctcggagatgtgtataagagacagCCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATAT < GWNJ‑0478:712:GW2002102894th:2:2209:3660:39633/107‑1 (MQ=60)
GAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATctgtc > GWNJ‑0478:712:GW2002102894th:2:1109:10196:44478/1‑145 (MQ=60)
GAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTAC < GWNJ‑0478:712:GW2002102894th:2:2114:4977:60465/150‑1 (MQ=60)
gggctcggagatgtgtataagagacaGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTAC < GWNJ‑0478:712:GW2002102894th:2:1215:9380:65027/123‑1 (MQ=60)
gaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCC < GWNJ‑0478:712:GW2002102894th:2:1115:17809:63225/90‑1 (MQ=60)
TGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCT > GWNJ‑0478:712:GW2002102894th:2:2204:13846:21186/1‑150 (MQ=60)
CGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAG < GWNJ‑0478:712:GW2002102894th:2:2101:12242:38774/149‑1 (MQ=60)
CCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATActgtctcttatacacatctccgagcccacgagac > GWNJ‑0478:712:GW2002102894th:2:2111:18326:65747/1‑116 (MQ=60)
GCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGA < GWNJ‑0478:712:GW2002102894th:2:2211:5842:27371/149‑1 (MQ=60)
GCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGG < GWNJ‑0478:712:GW2002102894th:2:1215:4368:23974/149‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacaGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGG < GWNJ‑0478:712:GW2002102894th:2:2110:18934:55177/119‑1 (MQ=60)
catacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagcccTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAA < GWNJ‑0478:712:GW2002102894th:2:2210:1625:49403/95‑1 (MQ=60)
TCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttg > GWNJ‑0478:712:GW2002102894th:2:1104:18550:65073/1‑84 (MQ=60)
GCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaccaaagagacgat > GWNJ‑0478:712:GW2002102894th:2:2115:14586:83241/1‑61 (MQ=60)
GCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGG > GWNJ‑0478:712:GW2002102894th:2:1208:16880:51596/1‑149 (MQ=60)
GTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCtgtctcttatacacatctccgagcccacgagacggagct > GWNJ‑0478:712:GW2002102894th:2:2113:9849:53177/1‑111 (MQ=60)
GCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGACGCAATGCGGTCGGTATG > GWNJ‑0478:712:GW2002102894th:2:1107:11531:98771/1‑150 (MQ=60)
|
GTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGGAAGACGAACGACGCAATGCGGTCGGTATG > AM260479/208809‑209096
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
