Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
564,366 |
C→G |
P73A (CCC→GCC) |
h16_A0537 → |
Uncharacterized protein conserved in bacteria |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 564,366 | 0 | C | G | 100.0%
| 37.1
/ NA
| 11 | P73A (CCC→GCC) | h16_A0537 | Uncharacterized protein conserved in bacteria |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (6/5); total (6/5) |
CCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGC > AM260479/564287‑564473
|
ccAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGGTGCCGACGCCTTGCGCCCGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCAcggg > 2:53829/1‑132 (MQ=255)
gccgcggccgcgGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAgc < 1:1165228/132‑1 (MQ=255)
aCTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCAcggc > 2:705540/1‑134 (MQ=255)
aCTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCAcg < 1:1018270/132‑1 (MQ=255)
aCTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCAcg > 2:1018270/1‑132 (MQ=255)
gACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTgccgc < 1:1162089/52‑1 (MQ=255)
gACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTgccgc > 2:1162089/1‑52 (MQ=255)
cGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCAc < 1:292554/65‑1 (MQ=255)
cGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCAc > 2:292554/1‑65 (MQ=255)
cAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTgccgc < 1:864108/40‑1 (MQ=255)
cAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTgccgc > 2:864108/1‑40 (MQ=255)
|
CCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGC > AM260479/564287‑564473
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CAGGCCACGCCTGCCTTCGCCAACGAACCGATGTGGTTTGCGCAGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACC > AM260479/564219‑564486
|
gcatacgagattagcgagtgtctcgtggggctcggagatgtgtataagagacaGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCC < GWNJ‑0478:712:GW2002102894th:2:1101:12444:79905/97‑1 (MQ=60)
tttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCAC < GWNJ‑0478:712:GW2002102894th:2:2116:20815:73546/70‑1 (MQ=60)
tcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCAC < GWNJ‑0478:712:GW2002102894th:2:2216:6133:71991/83‑1 (MQ=60)
cggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGC < GWNJ‑0478:712:GW2002102894th:2:1201:8805:54167/96‑1 (MQ=60)
GGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGG < GWNJ‑0478:712:GW2002102894th:2:2216:18844:85756/148‑1 (MQ=60)
tagcgagtgtctcgtgggctcggagatgtgtataagagacagGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGC < GWNJ‑0478:712:GW2002102894th:2:2212:21071:65954/107‑1 (MQ=60)
GCGACTACGATGCCGACGCCTTGCGCCAGGCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACC < GWNJ‑0478:712:GW2002102894th:2:2208:6746:79884/150‑1 (MQ=60)
|
CAGGCCACGCCTGCCTTCGCCAACGAACCGATGTGGTTTGCGCAGGGCCGACCGGTGCCGGCGGCCCGCCAGGCCGTCGATGCGCTGGCCGCGGCCGCGGCCGACGGCCTCGACCCGCGCGACTACGATGCCGACGCCTTGCGCCAGCCCGTGGCCCAGGCCGCCAGCGGGCCAGCATTGCCGCCGGACGCTGCCGCACGCGTGGATGCGGCGCTGAGCGCCGCGATGGAGCGGATGCTGGCAGACCTGCACGGCGGGCGCGTCAACC > AM260479/564219‑564486
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
