Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
581,821 |
+C |
coding (117/1734 nt) |
h16_A0555 → |
zinc metalloprotease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 581,820 | 1 | . | C | 100.0%
| 74.0
/ NA
| 20 | S39T (AGC→ACC) | h16_A0555 | zinc metalloprotease |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (11/9); total (11/9) |
GGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAG‑CACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGC > AM260479/581734‑581932
|
ggcggcggcCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTg > 1:207570/1‑134 (MQ=255)
cGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCcg < 2:690517/102‑1 (MQ=255)
cGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCcg > 1:690517/1‑102 (MQ=255)
cGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCAtc > 1:98428/1‑134 (MQ=255)
cTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCAtctc > 2:420892/1‑134 (MQ=255)
gcgcTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGc < 1:420892/132‑1 (MQ=255)
gcgcTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGc < 2:98428/132‑1 (MQ=255)
ggCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCg > 1:764820/1‑99 (MQ=255)
ggCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCg < 2:764820/99‑1 (MQ=255)
cAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCAtctc > 1:784632/1‑89 (MQ=255)
cAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCAtctc < 2:784632/89‑1 (MQ=255)
tACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTg < 1:723812/130‑1 (MQ=255)
tACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTg > 2:723812/1‑130 (MQ=255)
gcgcACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGc > 1:764784/1‑133 (MQ=255)
ggCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGc > 1:1036162/1‑62 (MQ=255)
ggCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGc < 2:1036162/62‑1 (MQ=255)
ggCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAg > 1:500142/1‑84 (MQ=255)
ggCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAg < 2:500142/84‑1 (MQ=255)
ggCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGa < 2:750073/99‑1 (MQ=255)
ggCCCCGGCGCCAGCCACGCCGGTGGCGGCTACGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGa > 1:750073/1‑99 (MQ=255)
|
GGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAG‑CACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGC > AM260479/581734‑581932
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGCGTCACATCGCGCAGGCGCCGGCCATTGCCGCGCTGGCGGCGCATGGACGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAG‑CACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGCCAGAAGTC > AM260479/581678‑581940
|
catacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCC < GWNJ‑0478:712:GW2002102894th:2:2202:15904:68195/99‑1 (MQ=60)
GCGCATGGACGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGC > GWNJ‑0478:712:GW2002102894th:2:1112:17887:16925/1‑150 (MQ=60)
CGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCctgtctcttatacacatc > GWNJ‑0478:712:GW2002102894th:2:2108:8012:5249/1‑132 (MQ=60)
CGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCT > GWNJ‑0478:712:GW2002102894th:2:1106:4046:15559/1‑150 (MQ=60)
GGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGAC < GWNJ‑0478:712:GW2002102894th:2:1209:16530:35938/148‑1 (MQ=60)
TCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGctgtctcttatacacatctcc > GWNJ‑0478:712:GW2002102894th:2:2110:7576:99036/1‑129 (MQ=60)
CGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGcgatccacatcgactggtcctgcgagg < GWNJ‑0478:712:GW2002102894th:2:1111:4918:31054/149‑28 (MQ=60)
GCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACtgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:2113:1389:96895/1‑120 (MQ=60)
gtgggctcggagatgtgtataagagacaGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGG < GWNJ‑0478:712:GW2002102894th:2:1112:18871:65000/122‑1 (MQ=60)
GGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAG‑C < GWNJ‑0478:712:GW2002102894th:2:1115:2738:72717/42‑1 (MQ=60)
GCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGCC > GWNJ‑0478:712:GW2002102894th:2:2110:13402:98881/1‑149 (MQ=60)
CAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGCCAGAAG < GWNJ‑0478:712:GW2002102894th:2:2109:11664:87594/150‑1 (MQ=60)
GGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtct > GWNJ‑0478:712:GW2002102894th:2:2211:15569:74622/1‑92 (MQ=60)
GGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACtgtctcttatacacatctccgagcccacgagacac > GWNJ‑0478:712:GW2002102894th:2:1212:13875:49572/1‑115 (MQ=60)
GGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTACGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCtgtctcttatacacatctcc > GWNJ‑0478:712:GW2002102894th:2:2110:2343:23075/1‑130 (MQ=60)
|
CGCGTCACATCGCGCAGGCGCCGGCCATTGCCGCGCTGGCGGCGCATGGACGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAG‑CACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGCCAGAAGTC > AM260479/581678‑581940
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
