Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
582,589 |
T→G |
H295Q (CAT→CAG) |
h16_A0555 → |
zinc metalloprotease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 582,589 | 0 | T | G | 100.0%
| 103.5
/ NA
| 27 | H295Q (CAT→CAG) | h16_A0555 | zinc metalloprotease |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (13/14); total (13/14) |
CGGCGCTGGCCATGGGCGGGCAGGGCGCGGCCATCGCCAACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCATGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTC > AM260479/582465‑582712
|
cggcgCTGGCCATGGGCGGGCAGGGCGCGGCCATCGCCAACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGccc > 1:661347/1‑134 (MQ=255)
gggCGCGGCCATCGCCAACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAgc > 1:968194/1‑134 (MQ=255)
tCGCCAACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCggg > 1:568412/1‑133 (MQ=255)
cAACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACcg < 2:726871/134‑1 (MQ=255)
cTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGaaaa < 2:240194/134‑1 (MQ=255)
tCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAAtt < 2:568412/133‑1 (MQ=255)
cgcgcgGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGaaaa < 2:619685/124‑1 (MQ=255)
cgcgcgGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGaaaa > 1:619685/1‑124 (MQ=255)
gcgcgGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCg < 2:968194/133‑1 (MQ=255)
cGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCa < 1:850076/90‑1 (MQ=255)
cGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCa > 2:850076/1‑90 (MQ=255)
ccGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATttctt < 1:297452/67‑1 (MQ=255)
ccGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATttctt > 2:297452/1‑67 (MQ=255)
cAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATttctt < 1:1137677/50‑1 (MQ=255)
cAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATttctt > 2:1137677/1‑50 (MQ=255)
aGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGAtt < 1:29266/134‑1 (MQ=255)
aGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGAtt < 2:887735/134‑1 (MQ=255)
aCCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGaaaa > 1:354804/1‑77 (MQ=255)
aCCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGaaaa < 2:354804/77‑1 (MQ=255)
ccGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATg > 1:453360/1‑134 (MQ=255)
cgcgGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGc < 1:729168/134‑1 (MQ=255)
cggcggGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAAtt < 2:864816/75‑1 (MQ=255)
cggcggGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAAtt > 1:864816/1‑75 (MQ=255)
cggcggGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAg > 2:454682/1‑134 (MQ=255)
gggTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACcg > 1:116627/1‑134 (MQ=255)
ggTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACcgc > 1:185439/1‑134 (MQ=255)
tGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTc < 1:454682/133‑1 (MQ=255)
|
CGGCGCTGGCCATGGGCGGGCAGGGCGCGGCCATCGCCAACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCATGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTC > AM260479/582465‑582712
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCCCGGACGCCGCGGCGGCGCTGGCCATGGGCGGGCAGGGCGCGGCCATCGCCAACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCATGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAG > AM260479/582450‑582727
|
GCCCGGACGCCGCGGCGGCGCTGGCCATGGGCGGGCAGGGCGCGGCCATCGCCAACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCG > GWNJ‑0478:712:GW2002102894th:2:2107:13267:34255/1‑150 (MQ=60)
gcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCG < GWNJ‑0478:712:GW2002102894th:2:1201:8003:84930/98‑1 (MQ=60)
GGCCATGGGCGGGCAGGGCGCGGCCATCGCCAACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCG > GWNJ‑0478:712:GW2002102894th:2:2209:12882:94504/1‑150 (MQ=60)
tttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCG < GWNJ‑0478:712:GW2002102894th:2:2215:19133:65782/80‑1 (MQ=60)
GGCTGGGCGCGGCCATCGCCAACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGG > GWNJ‑0478:712:GW2002102894th:2:1216:11767:69543/1‑149 (MQ=60)
cgtgggctcggagatgtgtataagagacagGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGAC < GWNJ‑0478:712:GW2002102894th:2:2116:2989:10504/120‑1 (MQ=60)
ACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGT > GWNJ‑0478:712:GW2002102894th:2:2102:17410:87782/1‑150 (MQ=60)
GGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTActgtctcttatacacatctccgagcccacgagacactcgcta > GWNJ‑0478:712:GW2002102894th:2:1204:6039:49265/1‑107 (MQ=60)
GCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGC > GWNJ‑0478:712:GW2002102894th:2:1210:14810:11200/1‑150 (MQ=60)
cggagatgtgtataagagacaGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGC < GWNJ‑0478:712:GW2002102894th:2:2204:17663:15184/127‑1 (MQ=60)
TCCAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGctgtctcttatacacatctccgagcccacgagacactcgctaatc > GWNJ‑0478:712:GW2002102894th:2:2116:8941:77203/1‑105 (MQ=60)
CAGATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGAC < GWNJ‑0478:712:GW2002102894th:2:1103:18387:88127/150‑1 (MQ=60)
ATCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGC > GWNJ‑0478:712:GW2002102894th:2:1108:10745:24486/1‑150 (MQ=60)
TCCTGACCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCG > GWNJ‑0478:712:GW2002102894th:2:1113:10073:3880/1‑150 (MQ=60)
CCGCGGCGGGGTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCC < GWNJ‑0478:712:GW2002102894th:2:2111:18696:15567/150‑1 (MQ=60)
GTTTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCtgtctcttatacacatctc > GWNJ‑0478:712:GW2002102894th:2:1112:18270:4676/1‑131 (MQ=60)
TTGACCCGCAGGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAG < GWNJ‑0478:712:GW2002102894th:2:1210:19966:18131/149‑1 (MQ=60)
|
GCCCGGACGCCGCGGCGGCGCTGGCCATGGGCGGGCAGGGCGCGGCCATCGCCAACCAGCTGTCGTTCTCGCGCGGGGCCGAGCGCGAGGCCGACCGGGTCGGCTTCCAGATCCTGACCGCGGCGGGGTTTGACCCGCATGGCATGCCCGATTTCTTCATGCGGCTGCAGCGCGCATCGGGGACCGCGGAAAATTCGGTCCCGTCTTATGTGCGGACTCACCCGCTGACCTCCGAGCGGATTGCCGACATGCAGGACCGCGTCAGCCATGCCAGCAAG > AM260479/582450‑582727
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
