Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
628,654 |
+C |
coding (744/1206 nt) |
h16_A0595 ← |
Uncharacterized Fe‑S protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 628,653 | 1 | . | C | 100.0%
| 61.2
/ NA
| 17 | T249A (ACG→GCG) | h16_A0595 | Uncharacterized Fe‑S protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (9/8); total (9/8) |
TGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGCAAGCGGCGCGCGT‑CAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGATCTCGCCCAG > AM260479/628527‑628776
|
tGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGT‑ccagcc < 1:996996/132‑6 (MQ=255)
tGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGT‑ccagcc > 2:996996/1‑127 (MQ=255)
cGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGgcg > 1:552589/1‑134 (MQ=255)
gCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTGGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGACCAGGCAGCGGTgg > 1:841261/1‑133 (MQ=255)
cGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGt > 1:238371/1‑134 (MQ=255)
ccTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGc > 1:207575/1‑105 (MQ=255)
ccTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGc < 2:207575/105‑1 (MQ=255)
ccTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTg > 1:527178/1‑112 (MQ=255)
ccTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTg < 2:527178/112‑1 (MQ=255)
cTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTcggccg < 2:841261/134‑1 (MQ=255)
gTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGt < 2:552589/133‑1 (MQ=255)
cAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGt > 1:670651/1‑95 (MQ=255)
cAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGt < 2:670651/95‑1 (MQ=255)
gagaTGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGAt > 1:231142/1‑134 (MQ=255)
atgcaGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGAtctc < 2:231142/130‑1 (MQ=255)
atgcaGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGAtctc < 2:238371/130‑1 (MQ=255)
ggcgCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGATCTCGCCCAg > 1:1156906/1‑133 (MQ=255)
gcgcgcGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGc > 1:783162/1‑71 (MQ=255)
gcgcgcGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGc < 2:783162/71‑1 (MQ=255)
|
TGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGCAAGCGGCGCGCGT‑CAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGATCTCGCCCAG > AM260479/628527‑628776
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACCATGGCCACATGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGCAAGCGGCGCGCGT‑CAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGATCTCGCCCAGGAA > AM260479/628505‑628779
|
ACCATGGCCACATGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTACCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGT‑CC > GWNJ‑0478:712:GW2002102894th:2:2201:4305:70092/1‑150 (MQ=60)
ATGGCCACATGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGC < GWNJ‑0478:712:GW2002102894th:2:1216:16474:81977/150‑1 (MQ=60)
CGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGC > GWNJ‑0478:712:GW2002102894th:2:1214:13656:96370/1‑150 (MQ=60)
ACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGA < GWNJ‑0478:712:GW2002102894th:2:2207:8862:60842/150‑1 (MQ=60)
GCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTGGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGACCAGGCAGCGGTGGC > GWNJ‑0478:712:GW2002102894th:2:2114:6163:67701/1‑149 (MQ=60)
GCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGActgtctcttatacacatctccgagcccacgagacact > GWNJ‑0478:712:GW2002102894th:2:1105:17672:12675/1‑113 (MQ=60)
GAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTC > GWNJ‑0478:712:GW2002102894th:2:1115:16967:72928/1‑150 (MQ=60)
CCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCtgtctcttatacac > GWNJ‑0478:712:GW2002102894th:2:1112:1900:17240/1‑136 (MQ=60)
CCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCctgtctct > GWNJ‑0478:712:GW2002102894th:2:1215:13393:76722/1‑142 (MQ=60)
CTTGTGCTCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCtgtctcttatacacatctccgagc > GWNJ‑0478:712:GW2002102894th:2:2107:6294:86211/1‑126 (MQ=60)
TCGATGGTCAGGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGATC > GWNJ‑0478:712:GW2002102894th:2:1115:11021:38220/1‑150 (MQ=60)
GGTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGATCTCGCCCAGG > GWNJ‑0478:712:GW2002102894th:2:2216:13872:49055/1‑149 (MQ=60)
GTAGGAGATGC‑AGCGGCGCGCGTCCAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgt > GWNJ‑0478:712:GW2002102894th:2:2113:19768:89740/1‑102 (MQ=60)
|
ACCATGGCCACATGCGCGAACTTGTTCCACGGGCAGGCCAGCTGGCAGTCGTCGCAGCCGTAGACGCGGTTGCCCATGGGCGCGCGGAACTCCACCGGAATCGGGCCCTTGTGCTCGATGGTCAGGTAGGAGATGCAAGCGGCGCGCGT‑CAGCCGGTACGGCGCCACGATGGCGCGCGTGGGGCAGATGTCCAGGCAGCGGTGGCAGTTGCCGCAGTGGCCGGACTCGGGGGCGTCGGCCGGCAGCGGGATGTCGACCAGGATCTCGCCCAGGAA > AM260479/628505‑628779
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
