Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,515,428 |
+C |
intergenic (+367/‑456) |
tRNA‑Pro (CGG) → / → pilB |
tRNA‑Pro/Type IV pilus assembly protein PilB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,515,427 | 1 | . | C | 100.0%
| 57.0
/ NA
| 16 | intergenic (+366/‑457) | tRNA‑Pro (CGG)/pilB | tRNA‑Pro/Type IV pilus assembly protein PilB |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (7/9); total (7/9) |
CGCGGCGGTGAAATCTGCGTTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGG‑CTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCAT > AM260479/3515296‑3515549
|
cgcgGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGG‑cct > 2:682270/1‑132 (MQ=255)
tGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAgg < 1:682270/134‑1 (MQ=255)
gTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATgg > 2:567054/1‑134 (MQ=255)
gCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCa < 1:828521/134‑1 (MQ=255)
cGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCtt > 1:308315/1‑134 (MQ=255)
gaAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCatgat > 1:537315/1‑108 (MQ=255)
gaAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCatgat < 2:537315/108‑1 (MQ=255)
tCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAgg > 1:3317/1‑134 (MQ=255)
cTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTtct < 1:488416/98‑1 (MQ=255)
cTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTtct > 2:488416/1‑98 (MQ=255)
gCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTgc < 1:567054/132‑1 (MQ=255)
cGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCcttctt < 1:402963/134‑1 (MQ=255)
cGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTtgat < 1:295162/134‑1 (MQ=255)
cTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCcttctt > 1:888404/1‑98 (MQ=255)
cTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCcttctt < 2:888404/98‑1 (MQ=255)
cTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCAt > 1:624584/1‑134 (MQ=255)
gCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCa < 2:624584/130‑1 (MQ=255)
|
CGCGGCGGTGAAATCTGCGTTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGG‑CTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCAT > AM260479/3515296‑3515549
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTATTCGGCCGCCTCCGCGGCGGTGAAATCTGCGTTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGG‑CTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCATCACGCAGTGTT > AM260479/3515281‑3515560
|
GTATTCGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGG‑Cctt > GWNJ‑0478:712:GW2002102894th:2:2110:14026:93945/1‑147 (MQ=60)
gagacaGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATC < GWNJ‑0478:712:GW2002102894th:2:2115:7455:91994/144‑1 (MQ=60)
CGGCCGCCTCCGCGGCGGTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATC < GWNJ‑0478:712:GW2002102894th:2:2104:18279:62924/149‑1 (MQ=60)
GTGAAATCTGC‑TTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATG < GWNJ‑0478:712:GW2002102894th:2:2106:12691:56634/150‑1 (MQ=60)
GGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTC > GWNJ‑0478:712:GW2002102894th:2:1203:15317:55158/1‑150 (MQ=60)
GGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTC < GWNJ‑0478:712:GW2002102894th:2:2114:6174:7991/150‑1 (MQ=60)
GACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCctgtctcttat > GWNJ‑0478:712:GW2002102894th:2:1214:2804:24083/1‑138 (MQ=60)
cggagatgtgtataagagacagGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGAT < GWNJ‑0478:712:GW2002102894th:2:1213:2563:89606/128‑1 (MQ=60)
TGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGT > GWNJ‑0478:712:GW2002102894th:2:1101:19543:23393/1‑150 (MQ=60)
CGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAAC < GWNJ‑0478:712:GW2002102894th:2:1216:5576:63494/148‑1 (MQ=60)
CCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaaaaaagtcgg > GWNJ‑0478:712:GW2002102894th:2:1109:10078:20703/1‑59 (MQ=60)
CCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAA < GWNJ‑0478:712:GW2002102894th:2:1208:19296:34291/150‑1 (MQ=60)
GCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGG < GWNJ‑0478:712:GW2002102894th:2:1201:12168:70076/150‑1 (MQ=60)
GCGCTCCTGCGCGGGCATCTTGCTGACGG‑Ccttcatcctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaacataagaaaaacatatcctgcatagaatcgct > GWNJ‑0478:712:GW2002102894th:2:2115:18196:68834/1‑30 (MQ=60)
CTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAActgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:2203:18092:21390/1‑128 (MQ=60)
CTCCTGCGCGGGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCATC > GWNJ‑0478:712:GW2002102894th:2:2105:17290:18345/1‑150 (MQ=60)
GGCATCTTGCTGACGGCCTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCATCACGCAGTGTT > GWNJ‑0478:712:GW2002102894th:2:2110:19387:40742/1‑150 (MQ=60)
|
GTATTCGGCCGCCTCCGCGGCGGTGAAATCTGCGTTCCGCGAGGTGGCCAAAGACTTCCTCGTGCGAGAAGTCCTCGTCCTTGAAAGCCTGCTCGCCCACCGGCGTGAAAAACTTGATGCGCTCCTGCGCGGGCATCTTGCTGACGG‑CTTCATCAGGTCTTCTGCGGTCATGATGGTCATCCCTTTCTCTCTTCATCCTCGATCGCCAGGTGCAGCGGCTTGTGCGCCTTCTTGATGAACTGCATGAAGGGCTTCCTGCAAATTGGCATCACGCAGTGTT > AM260479/3515281‑3515560
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
