Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,274,430 |
C→G |
G565G (GGG→GGC) |
h16_A2092 ← |
Lysophospholipase, contains weak SAM‑dependent methyltransferase domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,274,430 | 0 | C | G | 100.0%
| 88.9
/ NA
| 24 | G565G (GGG→GGC) | h16_A2092 | Lysophospholipase, contains weak SAM‑dependent methyltransferase domain |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (9/15); total (9/15) |
TGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGG > AM260479/2274301‑2274557
|
tGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAg > 2:30061/1‑134 (MQ=255)
gCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAg < 1:262344/133‑1 (MQ=255)
gCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAg < 2:817667/133‑1 (MQ=255)
gCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAg < 1:869322/133‑1 (MQ=255)
gCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAg < 2:583132/133‑1 (MQ=255)
gcagCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCa < 2:913905/134‑1 (MQ=255)
tGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTcc < 1:30061/134‑1 (MQ=255)
tGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTcc < 2:653525/134‑1 (MQ=255)
tCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAgg > 2:862871/1‑134 (MQ=255)
tCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAgg > 2:619759/1‑134 (MQ=255)
cATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCAt < 2:936821/125‑1 (MQ=255)
cATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCAt > 1:936821/1‑125 (MQ=255)
gccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgccgctc > 2:119096/1‑131 (MQ=255)
gccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgccg < 1:6920/131‑1 (MQ=255)
gccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgccg > 2:6920/1‑131 (MQ=255)
tGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCgg > 1:301218/1‑134 (MQ=255)
cgcCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGc < 1:133173/134‑1 (MQ=255)
tGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGcc > 1:109578/1‑47 (MQ=255)
tGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGcc < 2:109578/47‑1 (MQ=255)
aTACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCgg < 1:211306/134‑1 (MQ=255)
tACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAgcggcgg < 2:488554/68‑1 (MQ=255)
tACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAgcggcgg > 1:488554/1‑68 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGc < 2:301218/134‑1 (MQ=255)
tCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCgg < 1:499533/133‑1 (MQ=255)
|
TGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGG > AM260479/2274301‑2274557
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CAGGGCCGGTTGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACCAGATAGC > AM260479/2274291‑2274572
|
gctttatttgtttttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCC < GWNJ‑0478:712:GW2002102894th:2:2101:19798:87172/58‑1 (MQ=60)
TGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTT < GWNJ‑0478:712:GW2002102894th:2:1201:11582:68924/149‑1 (MQ=60)
TGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTT < GWNJ‑0478:712:GW2002102894th:2:2206:14492:76200/149‑1 (MQ=60)
ATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAG < GWNJ‑0478:712:GW2002102894th:2:1105:13186:8495/150‑1 (MQ=60)
CGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCG > GWNJ‑0478:712:GW2002102894th:2:2210:9247:86640/1‑150 (MQ=60)
CTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGT > GWNJ‑0478:712:GW2002102894th:2:1205:2300:35305/1‑150 (MQ=60)
CCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTG < GWNJ‑0478:712:GW2002102894th:2:1101:8496:51106/150‑1 (MQ=60)
CGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGctcgcaggccgagatggacgaact < GWNJ‑0478:712:GW2002102894th:2:1108:18208:91110/150‑25 (MQ=60)
GCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCG < GWNJ‑0478:712:GW2002102894th:2:1109:18917:79085/150‑1 (MQ=60)
GGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1108:8499:31910/1‑77 (MQ=60)
TGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatg > GWNJ‑0478:712:GW2002102894th:2:1214:4242:49770/1‑98 (MQ=60)
gtataagagacagGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGC < GWNJ‑0478:712:GW2002102894th:2:2110:21050:99262/137‑1 (MQ=60)
GATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCC < GWNJ‑0478:712:GW2002102894th:2:1115:4701:45563/150‑1 (MQ=60)
GGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGccgcctgggtctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2111:7937:96820/1‑61 (MQ=60)
GTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGctgtctcttatacacatc > GWNJ‑0478:712:GW2002102894th:2:2102:14531:40425/1‑132 (MQ=60)
GTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACCAGATAGC < GWNJ‑0478:712:GW2002102894th:2:1216:14145:12439/149‑1 (MQ=60)
|
CAGGGCCGGTTGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACCAGATAGC > AM260479/2274291‑2274572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
