Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,274,430 |
C→G |
G565G (GGG→GGC) |
h16_A2092 ← |
Lysophospholipase, contains weak SAM‑dependent methyltransferase domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,274,430 | 0 | C | G | 96.2%
| 89.9
/ ‑3.6
| 26 | G565G (GGG→GGC) | h16_A2092 | Lysophospholipase, contains weak SAM‑dependent methyltransferase domain |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (16/9); minor base A (1/0); total (17/9) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 |
GGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGT > AM260479/2274329‑2274558
|
ggCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGc > 1:157444/1‑134 (MQ=255)
cTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAgcg > 2:111030/1‑134 (MQ=255)
ggCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCT‑GGTCACCTTGTCAAAACCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCa > 1:272298/1‑134 (MQ=255)
tCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAgg > 1:743289/1‑134 (MQ=255)
cATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGc < 2:499275/107‑1 (MQ=255)
cATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGc > 1:499275/1‑107 (MQ=255)
cATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAggc > 1:641394/1‑134 (MQ=255)
gccgcgccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAg > 2:943761/1‑74 (MQ=255)
gccgcgccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAg < 1:943761/74‑1 (MQ=255)
cgcgccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgccg < 2:157444/134‑1 (MQ=255)
gccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgccggcg > 1:70340/1‑134 (MQ=255)
gccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgc > 1:178444/1‑129 (MQ=255)
gccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgc < 2:178444/129‑1 (MQ=255)
tGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCgg > 1:110603/1‑134 (MQ=255)
cgcgcCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTg > 1:153788/1‑134 (MQ=255)
tGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAgcgcgcgc < 1:111030/134‑1 (MQ=255)
cACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTc > 2:390913/1‑134 (MQ=255)
cACGGGTCGATACGATGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTc > 1:1152927/1‑134 (MQ=255)
gggTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAg > 1:639852/1‑134 (MQ=255)
ggTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGc < 1:390913/134‑1 (MQ=255)
ggTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGc < 2:110603/134‑1 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGc > 1:176840/1‑134 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGc > 2:1124602/1‑134 (MQ=255)
cTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGc < 2:153788/134‑1 (MQ=255)
gTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGcc < 2:743289/132‑1 (MQ=255)
tCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGgt > 1:187005/1‑134 (MQ=255)
|
GGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGT > AM260479/2274329‑2274558
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TTGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACCAGATAGC > AM260479/2274300‑2274572
|
agattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTT < GWNJ‑0478:712:GW2002102894th:2:2205:20054:56908/105‑1 (MQ=60)
CTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCC > GWNJ‑0478:712:GW2002102894th:2:1111:5565:33186/1‑150 (MQ=60)
GCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCT‑GGTCACCTTGTCAAAACCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCAT > GWNJ‑0478:712:GW2002102894th:2:1116:10443:21061/1‑150 (MQ=60)
CCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGC > GWNJ‑0478:712:GW2002102894th:2:2111:15133:56484/1‑150 (MQ=60)
CGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCtgtctcttatac > GWNJ‑0478:712:GW2002102894th:2:1213:15410:97079/1‑138 (MQ=60)
CGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCG > GWNJ‑0478:712:GW2002102894th:2:2105:2265:69537/1‑150 (MQ=60)
ttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGG < GWNJ‑0478:712:GW2002102894th:2:1104:13768:82400/75‑1 (MQ=60)
GTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGC > GWNJ‑0478:712:GW2002102894th:2:1104:5014:38385/1‑150 (MQ=60)
GTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGC > GWNJ‑0478:712:GW2002102894th:2:1110:5814:45128/1‑150 (MQ=60)
CTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGT > GWNJ‑0478:712:GW2002102894th:2:1106:7074:73098/1‑150 (MQ=60)
CATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGG > GWNJ‑0478:712:GW2002102894th:2:1111:14076:14061/1‑150 (MQ=60)
ACGGTGAAGATGCCCCACGGGTCGATACGATGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCC > GWNJ‑0478:712:GW2002102894th:2:2216:2053:50451/1‑150 (MQ=60)
GTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGC > GWNJ‑0478:712:GW2002102894th:2:2105:5606:60694/1‑150 (MQ=60)
GTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGC < GWNJ‑0478:712:GW2002102894th:2:1106:2816:75665/150‑1 (MQ=60)
GGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGC < GWNJ‑0478:712:GW2002102894th:2:1206:6398:28321/150‑1 (MQ=60)
GGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGgtgccgcccggcggctatctggtctac > GWNJ‑0478:712:GW2002102894th:2:2215:18866:93409/1‑122 (MQ=60)
GGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCT > GWNJ‑0478:712:GW2002102894th:2:1110:5188:36950/1‑150 (MQ=60)
CGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTG > GWNJ‑0478:712:GW2002102894th:2:1110:1809:90940/1‑150 (MQ=60)
GGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGctgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:2210:8261:58745/1‑127 (MQ=60)
TGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACCAGATAGC > GWNJ‑0478:712:GW2002102894th:2:1208:3318:82011/1‑150 (MQ=60)
|
TTGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACCAGATAGC > AM260479/2274300‑2274572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
