Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,248,092 |
G→C |
intergenic (+43/‑175) |
h16_A3001 → / → hemK1 |
transcriptional regulator, LysR‑family/methylase of polypeptide chain release factors |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,248,092 | 0 | G | C | 100.0%
| 89.0
/ NA
| 24 | intergenic (+43/‑175) | h16_A3001/hemK1 | transcriptional regulator, LysR‑family/methylase of polypeptide chain release factors |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (11/13); total (11/13) |
CCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCC > AM260479/3247991‑3248203
|
gcgcgA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCa < 2:869438/133‑1 (MQ=255)
cgcgA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCaa > 2:939984/1‑134 (MQ=255)
cgaccGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAAcc < 1:633108/131‑1 (MQ=255)
gCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAg > 2:188538/1‑134 (MQ=255)
aCCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGaa > 2:309443/1‑134 (MQ=255)
ccTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAac < 1:309443/134‑1 (MQ=255)
gcagcaA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGcc < 1:939984/134‑1 (MQ=255)
gcagcaA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGcc < 2:867099/134‑1 (MQ=255)
cagcaA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCt < 1:936139/134‑1 (MQ=255)
aacGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGcc < 1:931033/127‑1 (MQ=255)
aacGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGcc > 2:931033/3‑129 (MQ=255)
cGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCa < 1:496021/83‑1 (MQ=255)
cGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCa > 2:496021/1‑83 (MQ=255)
gtcaagcagGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCccgc > 2:579075/5‑134 (MQ=255)
gtcaagcagGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCccgc > 2:397885/5‑134 (MQ=255)
cagGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCctc < 1:924503/134‑1 (MQ=255)
cagGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCctc < 1:397885/134‑1 (MQ=255)
gggTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCt > 2:431049/1‑57 (MQ=255)
gggTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCt < 1:431049/57‑1 (MQ=255)
gTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTccc > 1:172751/1‑134 (MQ=255)
gCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCccgc > 2:392306/1‑109 (MQ=255)
gCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCccgc < 1:392306/109‑1 (MQ=255)
gCTTTGTCTACACCTTCATCGAGCTGTTCTCACCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCctc > 1:974326/1‑115 (MQ=255)
gCTTTGTCTACACCTTCATCGAGCTGTTCTCACCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCctc < 2:974326/115‑1 (MQ=255)
|
CCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCC > AM260479/3247991‑3248203
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGGCTGGGCGTGGGATTCGTCGCCGGCGTCGCCTATGATGCCCGAGCCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACCCAACCATGCATCTGCTGCC > AM260479/3247945‑3248239
|
GGGCTGGGCGTGGGCATCGTCGCCGGCGTCGCCTATGATG‑CCGAG‑CGCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACC < GWNJ‑0478:712:GW2002102894th:2:2106:14785:11181/150‑1 (MQ=60)
gactctcttggtctctgctttttttcttcgtttttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGC < GWNJ‑0478:712:GW2002102894th:2:1213:5658:46503/40‑1 (MQ=60)
gcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGT < GWNJ‑0478:712:GW2002102894th:2:1213:16261:30998/87‑1 (MQ=60)
ccgtctcgtgggctcggagatgtgtataagagacagCCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTG < GWNJ‑0478:712:GW2002102894th:2:1214:19841:91754/113‑1 (MQ=60)
GCGA‑CCGCAACC‑TGCGCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGC < GWNJ‑0478:712:GW2002102894th:2:2109:1701:5628/150‑1 (MQ=60)
tttttttttttttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGC < GWNJ‑0478:712:GW2002102894th:2:2106:4875:28510/60‑1 (MQ=60)
ACCTGTTTGGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAAC < GWNJ‑0478:712:GW2002102894th:2:1205:20415:87371/150‑1 (MQ=60)
GGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGA < GWNJ‑0478:712:GW2002102894th:2:2212:5601:6254/150‑1 (MQ=60)
GGCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGA < GWNJ‑0478:712:GW2002102894th:2:2210:17137:52152/150‑1 (MQ=60)
GCAGCAA‑CGTGACGCACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGAC < GWNJ‑0478:712:GW2002102894th:2:2210:6162:82582/150‑1 (MQ=60)
CACCTGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCtgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaagagcagacaggtaatacac > GWNJ‑0478:712:GW2002102894th:2:2109:4893:14997/1‑54 (MQ=60)
TGGCCGTCAAGCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCT > GWNJ‑0478:712:GW2002102894th:2:1113:17352:18249/1‑150 (MQ=60)
ataagagacaGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATAT < GWNJ‑0478:712:GW2002102894th:2:1211:12647:9394/140‑1 (MQ=60)
GCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAG < GWNJ‑0478:712:GW2002102894th:2:1211:9211:41642/150‑1 (MQ=60)
GCAGGGTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAG < GWNJ‑0478:712:GW2002102894th:2:2210:9435:14066/150‑1 (MQ=60)
GTGCCTACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCACCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCtgtc > GWNJ‑0478:712:GW2002102894th:2:2214:16419:2770/1‑146 (MQ=60)
TACCTGCGCAGCTTTGTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACCCAAC > GWNJ‑0478:712:GW2002102894th:2:1113:8694:46814/1‑150 (MQ=60)
GTCTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACCCAACCATGCATCTGCTGCC < GWNJ‑0478:712:GW2002102894th:2:1110:14649:89087/150‑1 (MQ=60)
|
GGGCTGGGCGTGGGATTCGTCGCCGGCGTCGCCTATGATGCCCGAGCCGCGACCCGCAACCTTGCCCGGCATCTCGGCCGGCCACCTGTTTGGCAGCAACCGTGACGCACCTGGCCGTC‑AGCAGGGTGCCTACCTGCGCAGCTTTGTGTACACCTTCATCGAGCTGTTCTCGCCGACGCTCAACCGCAAGCTGGTTGAGCAGGCCATGTCCGGCGAACACGAAGCCTACGAACTCTGAGGACGGCCCGCCGCCTCTCCCTATATAAAAAGCAAACCCAACCATGCATCTGCTGCC > AM260479/3247945‑3248239
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
