Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,285,635 |
G→C |
G181R (GGC→CGC) |
nadB → |
L‑Aspartate oxidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,285,635 | 0 | G | C | 100.0%
| 114.4
/ NA
| 31 | G181R (GGC→CGC) | nadB | L‑Aspartate oxidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (15/16); total (15/16) |
GCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCGGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATGG > AM260479/3285505‑3285749
|
gcagcaGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCcgcg > 1:259940/1‑134 (MQ=255)
aTGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCAc > 1:993494/1‑134 (MQ=255)
gcaCCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGt > 1:808963/1‑123 (MQ=255)
gcaCCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGt < 2:808963/123‑1 (MQ=255)
ccACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCgcgg > 1:1154205/1‑134 (MQ=255)
ttCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCt > 2:280728/1‑101 (MQ=255)
ttCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCt < 1:280728/101‑1 (MQ=255)
gCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTg < 1:1075687/133‑1 (MQ=255)
tCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGc < 1:353090/134‑1 (MQ=255)
ccTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAg > 2:811951/1‑133 (MQ=255)
ccTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAg < 1:811951/133‑1 (MQ=255)
cTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGt < 2:259940/134‑1 (MQ=255)
gATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCAcc > 1:142819/1‑85 (MQ=255)
gATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCAcc < 2:142819/85‑1 (MQ=255)
gATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTc > 1:15497/1‑133 (MQ=255)
aCCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCt < 1:734449/134‑1 (MQ=255)
cAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGc < 2:908451/111‑1 (MQ=255)
cAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGc > 1:908451/1‑111 (MQ=255)
ggCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGc < 1:658117/78‑1 (MQ=255)
ggCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGc > 2:658117/1‑78 (MQ=255)
ggCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCg > 1:1113132/1‑134 (MQ=255)
ggCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCg > 1:564449/1‑134 (MQ=255)
gCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCTGGCGGTGCCGGc < 2:632647/98‑1 (MQ=255)
gCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCTGGCGGTGCCGGc > 1:632647/1‑98 (MQ=255)
ctACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGAc < 2:993494/133‑1 (MQ=255)
ggCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCAt < 2:1113132/134‑1 (MQ=255)
ggCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCAt < 2:564449/134‑1 (MQ=255)
cgtacTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATgg > 2:783122/1‑134 (MQ=255)
cgtacTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATgg > 2:964712/1‑134 (MQ=255)
gacgacAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGc < 1:670615/65‑1 (MQ=255)
gacgacAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGc > 2:670615/1‑65 (MQ=255)
aCCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGc < 1:480806/60‑1 (MQ=255)
|
GCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCGGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATGG > AM260479/3285505‑3285749
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGTCAGCACGCTGCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCGGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATGGCCTGGCGCGCGGGCTGCCGGGTTTC > AM260479/3285490‑3285774
|
GGTCAGCACGCTGCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGC > GWNJ‑0478:712:GW2002102894th:2:1114:4391:59175/1‑150 (MQ=60)
gtctcgtgggctcggagatgtgtataagagacagCATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTG < GWNJ‑0478:712:GW2002102894th:2:2212:14434:19457/115‑1 (MQ=60)
GTGCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACC < GWNJ‑0478:712:GW2002102894th:2:2203:12008:36073/150‑1 (MQ=60)
CTGCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACC > GWNJ‑0478:712:GW2002102894th:2:2207:7581:41155/1‑150 (MQ=60)
GCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCC < GWNJ‑0478:712:GW2002102894th:2:2205:7300:20360/149‑1 (MQ=60)
GCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACC < GWNJ‑0478:712:GW2002102894th:2:2114:18416:67969/148‑1 (MQ=60)
GCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTgtc > GWNJ‑0478:712:GW2002102894th:2:1116:10992:23882/1‑147 (MQ=60)
GCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCAC > GWNJ‑0478:712:GW2002102894th:2:2112:12015:98805/1‑150 (MQ=60)
agatgtgtataagagacagATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACAC < GWNJ‑0478:712:GW2002102894th:2:1116:21239:64572/131‑1 (MQ=60)
gagttcttgctgtgactctttctcttttttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCAC < GWNJ‑0478:712:GW2002102894th:2:2114:16306:74536/39‑1 (MQ=60)
agcgagtgtctcgtgggctcggagatgtgtataagagacagCCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCG < GWNJ‑0478:712:GW2002102894th:2:2104:3065:72063/108‑1 (MQ=60)
CACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGG > GWNJ‑0478:712:GW2002102894th:2:2216:15849:56074/1‑150 (MQ=60)
CCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGcctgtctcttatacacatctccgagcccacgagacactcgctaatct > GWNJ‑0478:712:GW2002102894th:2:1215:15813:19642/1‑103 (MQ=60)
CTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACctgtctcttatacacatctccgagcccacgagac > GWNJ‑0478:712:GW2002102894th:2:1109:14659:55040/1‑115 (MQ=60)
CTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCT > GWNJ‑0478:712:GW2002102894th:2:1103:11591:2130/1‑149 (MQ=60)
cggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCT < GWNJ‑0478:712:GW2002102894th:2:2107:14065:45796/95‑1 (MQ=60)
CGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACC < GWNJ‑0478:712:GW2002102894th:2:2212:12094:80429/149‑1 (MQ=60)
gcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTG < GWNJ‑0478:712:GW2002102894th:2:1101:20704:85471/87‑1 (MQ=60)
gaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTAC < GWNJ‑0478:712:GW2002102894th:2:1213:12020:9328/90‑1 (MQ=60)
ATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTAC < GWNJ‑0478:712:GW2002102894th:2:1204:7199:8917/150‑1 (MQ=60)
GACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACC < GWNJ‑0478:712:GW2002102894th:2:2115:8040:14347/150‑1 (MQ=60)
CCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTctgtctctt > GWNJ‑0478:712:GW2002102894th:2:2202:12814:44385/1‑141 (MQ=60)
CACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCC < GWNJ‑0478:712:GW2002102894th:2:2111:9640:10196/150‑1 (MQ=60)
CCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGG > GWNJ‑0478:712:GW2002102894th:2:1216:7940:9039/1‑150 (MQ=60)
CCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGG > GWNJ‑0478:712:GW2002102894th:2:2214:18401:63344/1‑150 (MQ=60)
AGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCTGGCGGTGCCGGCAAGGTCTACCTGTACctgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:2105:19647:18094/1‑128 (MQ=60)
GACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCActgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaccac > GWNJ‑0478:712:GW2002102894th:2:1215:2094:88549/1‑67 (MQ=60)
|
GGTCAGCACGCTGCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCGGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATGGCCTGGCGCGCGGGCTGCCGGGTTTC > AM260479/3285490‑3285774
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
