Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,285,635 |
G→C |
G181R (GGC→CGC) |
nadB → |
L‑Aspartate oxidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,285,635 | 0 | G | C | 100.0%
| 90.2
/ NA
| 24 | G181R (GGC→CGC) | nadB | L‑Aspartate oxidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (12/12); total (12/12) |
AGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCGGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACC > AM260479/3285510‑3285712
|
agGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTg > 2:574934/1‑134 (MQ=255)
aTGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCAc > 1:115278/1‑134 (MQ=255)
aTGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCAc > 1:258458/1‑134 (MQ=255)
aTGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCAc > 2:571632/1‑134 (MQ=255)
cagcaCCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCt < 2:791044/134‑1 (MQ=255)
aCATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACg < 1:574934/134‑1 (MQ=255)
aTCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCcgcgcg < 1:1011879/111‑1 (MQ=255)
aTCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCcgcgcg > 2:1011879/1‑111 (MQ=255)
ccTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGc > 2:68773/1‑134 (MQ=255)
ccTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGc > 1:678148/1‑134 (MQ=255)
ggAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACg > 1:288013/1‑121 (MQ=255)
ggAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACg < 2:288013/121‑1 (MQ=255)
tCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGc < 2:272598/134‑1 (MQ=255)
tCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGc < 1:68773/134‑1 (MQ=255)
cGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGc > 2:420393/1‑133 (MQ=255)
cTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGt < 1:571632/134‑1 (MQ=255)
gcgcAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTac < 2:258458/133‑1 (MQ=255)
aaGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTAcaccac < 2:115278/134‑1 (MQ=255)
ggggCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTAcc < 1:657537/119‑1 (MQ=255)
ggggCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTAcc > 2:657537/1‑119 (MQ=255)
ggCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACaccacc > 2:614362/1‑128 (MQ=255)
ggCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACaccacc < 1:614362/128‑1 (MQ=255)
gacAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCgcgggc < 1:428749/57‑1 (MQ=255)
gacAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCgcgggc > 2:428749/1‑57 (MQ=255)
cAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTg > 2:414925/1‑59 (MQ=255)
cAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTg < 1:414925/59‑1 (MQ=255)
|
AGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCGGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACC > AM260479/3285510‑3285712
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTGGTCAGCACGCTGCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCGGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATGGCCTGGCGCGCGGGCTGCCGGGTTTCGAACAT > AM260479/3285488‑3285780
|
GTGGTCAGCACGCTGCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGC > GWNJ‑0478:712:GW2002102894th:2:1203:14892:44938/1‑150 (MQ=60)
AGCACGCTGCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTG < GWNJ‑0478:712:GW2002102894th:2:2106:11544:79387/150‑1 (MQ=60)
agctccgtctcgtgggctcggagatgtgtatagacagTCCTGGAAGACCACTTCGCAATCGACCTTATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACC < GWNJ‑0478:712:GW2002102894th:2:2208:14588:53102/113‑1 (MQ=60)
CTGCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACC > GWNJ‑0478:712:GW2002102894th:2:1108:20892:66942/1‑150 (MQ=60)
CTGCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACC > GWNJ‑0478:712:GW2002102894th:2:1201:21202:42218/1‑150 (MQ=60)
GCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACC < GWNJ‑0478:712:GW2002102894th:2:2208:4857:50883/148‑1 (MQ=60)
aagagacagATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACC < GWNJ‑0478:712:GW2002102894th:2:2216:18519:15742/141‑1 (MQ=60)
CCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCT > GWNJ‑0478:712:GW2002102894th:2:2110:20625:74466/1‑150 (MQ=60)
GAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGC > GWNJ‑0478:712:GW2002102894th:2:1202:16781:48088/1‑150 (MQ=60)
AACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCC < GWNJ‑0478:712:GW2002102894th:2:2104:16293:26192/150‑1 (MQ=60)
gcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTC < GWNJ‑0478:712:GW2002102894th:2:1213:8290:18743/87‑1 (MQ=60)
CTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCtgtctcttatacacatctccgagcccacgagacggagctacatctcg > GWNJ‑0478:712:GW2002102894th:2:1213:16725:21676/1‑103 (MQ=60)
agaagacggcatacgatatgtagctccgtctcgtgggctcggagatgtgtataagagacagGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACC < GWNJ‑0478:712:GW2002102894th:2:1210:14883:38860/89‑1 (MQ=60)
ATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTAC < GWNJ‑0478:712:GW2002102894th:2:1104:8074:70494/150‑1 (MQ=60)
CCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCAC < GWNJ‑0478:712:GW2002102894th:2:2104:19540:4393/150‑1 (MQ=60)
GCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACC < GWNJ‑0478:712:GW2002102894th:2:2111:12698:51966/150‑1 (MQ=60)
AAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCC < GWNJ‑0478:712:GW2002102894th:2:2106:12610:83598/150‑1 (MQ=60)
ACAGCACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgc > GWNJ‑0478:712:GW2002102894th:2:1212:7115:8573/1‑97 (MQ=60)
CACCCGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATGGCCTGGCGCGCGGGCTGCCGGGTTTCGAACAT > GWNJ‑0478:712:GW2002102894th:2:1215:18400:23316/1‑150 (MQ=60)
|
GTGGTCAGCACGCTGCTGCAGCAGGCCATGCAGCACCCGAACATCACCATCCTGGAAGACCACTTCGCAATCGACCTGATCACCTCGCGCAAGCTGGGGCTGCCGGGCAACCGCTGCTACGGCCTGTACGTACTGGACGACAGCACCGGCGCGGTGCGCACCCTCACCGCCACGCACACCGTGCTGGCCGCGGGCGGTGCCGGCAAGGTCTACCTGTACACCACCAACCCGGATACGGCCACCGGCGACGGCATCGCCATGGCCTGGCGCGCGGGCTGCCGGGTTTCGAACAT > AM260479/3285488‑3285780
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
