Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
919,006 |
T→G |
C159G (TGC→GGC) |
h16_B0812 → |
hydrolase or acyltransferase (alpha/beta hydrolase superfamily) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 919,006 | 0 | T | G | 100.0%
| 33.8
/ NA
| 10 | C159G (TGC→GGC) | h16_B0812 | hydrolase or acyltransferase (alpha/beta hydrolase superfamily) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (6/4); total (6/4) |
GGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAG > AM260480/918918‑919122
|
ggTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGAcc > 1:509633/1‑133 (MQ=255)
tCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGctgcgcc < 2:956025/133‑1 (MQ=255)
ggCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGctgcgcc < 1:123641/129‑1 (MQ=255)
ggCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGctgcgcc > 2:123641/1‑129 (MQ=255)
aCTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACg > 1:16149/1‑131 (MQ=255)
tCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACcg > 1:917035/1‑63 (MQ=255)
tCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACcg < 2:917035/63‑1 (MQ=255)
tCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAg > 1:725985/1‑134 (MQ=255)
ccTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGctgcgcctgcgc > 1:800432/1‑79 (MQ=255)
ccTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGctgcgcctgcgc < 2:800432/79‑1 (MQ=255)
|
GGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAG > AM260480/918918‑919122
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ATCGGCGTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACGACAT > AM260480/918865‑919142
|
ATCGGCGTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCGGGGCACCGAC < GWNJ‑0478:712:GW2002102894th:2:2105:15294:23769/150‑1 (MQ=60)
GGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCG > GWNJ‑0478:712:GW2002102894th:2:1216:6196:71912/1‑149 (MQ=60)
GTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGC < GWNJ‑0478:712:GW2002102894th:2:1109:20946:19992/149‑1 (MQ=60)
CGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGA > GWNJ‑0478:712:GW2002102894th:2:1103:16222:14659/1‑147 (MQ=60)
GGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtc > GWNJ‑0478:712:GW2002102894th:2:2211:2990:69743/1‑93 (MQ=60)
GGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGG > GWNJ‑0478:712:GW2002102894th:2:2115:5829:59226/1‑150 (MQ=60)
TGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATctgtctcttatacacatctccgagcccacgagacggagct > GWNJ‑0478:712:GW2002102894th:2:2202:18313:34053/1‑109 (MQ=60)
GTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaaaccaaa > GWNJ‑0478:712:GW2002102894th:2:2105:15216:74875/1‑65 (MQ=44)
|
ATCGGCGTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACGACAT > AM260480/918865‑919142
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
