Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
919,006 |
T→G |
C159G (TGC→GGC) |
h16_B0812 → |
hydrolase or acyltransferase (alpha/beta hydrolase superfamily) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 919,006 | 0 | T | G | 100.0%
| 81.7
/ NA
| 22 | C159G (TGC→GGC) | h16_B0812 | hydrolase or acyltransferase (alpha/beta hydrolase superfamily) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (10/12); total (10/12) |
CGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCG > AM260480/918897‑919133
|
cggcctggcCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGcgcc < 2:623537/134‑1 (MQ=255)
ccAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGc > 1:708108/1‑134 (MQ=255)
gTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACcg < 2:1129961/133‑1 (MQ=255)
aCCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGAc < 2:1028328/92‑1 (MQ=255)
aCCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGAc > 1:1028328/1‑92 (MQ=255)
aCCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt < 2:759269/112‑1 (MQ=255)
aCCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt > 1:759269/1‑112 (MQ=255)
ccgGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGct > 1:925063/1‑134 (MQ=255)
ccgGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGct > 2:377560/1‑134 (MQ=255)
gggCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGTCCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGa < 1:391004/134‑1 (MQ=255)
cgccgcGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATg > 1:607232/1‑134 (MQ=255)
gccgcGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATgg < 1:817894/134‑1 (MQ=255)
ccGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGAc > 1:586183/1‑45 (MQ=255)
ccGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGAc < 2:586183/45‑1 (MQ=255)
cGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt < 2:1117902/64‑1 (MQ=255)
cGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt > 1:1117902/1‑64 (MQ=255)
gTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCgtgg < 2:708108/134‑1 (MQ=255)
cGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAgg < 1:377560/133‑1 (MQ=255)
gCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACcg < 1:712620/60‑1 (MQ=255)
gCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACcg > 2:712620/1‑60 (MQ=255)
ccTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGAtt > 1:336415/1‑134 (MQ=255)
cgcCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATcg < 1:971622/134‑1 (MQ=255)
|
CGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCG > AM260480/918897‑919133
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACGACATCCATGC > AM260480/918873‑919148
|
GCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGG < GWNJ‑0478:712:GW2002102894th:2:2102:15144:21483/150‑1 (MQ=60)
GGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCC > GWNJ‑0478:712:GW2002102894th:2:2108:17640:64500/1‑150 (MQ=60)
GGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACtgtctcttatacacatctccgagcccacgagacactcg > GWNJ‑0478:712:GW2002102894th:2:1116:9675:38115/1‑112 (MQ=60)
GACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCtgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:2211:8000:34728/1‑123 (MQ=60)
GACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCtgtctct > GWNJ‑0478:712:GW2002102894th:2:2110:2472:41415/1‑143 (MQ=60)
GGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCtgtctcttatacacatctccgagcccacgagacactcg > GWNJ‑0478:712:GW2002102894th:2:1206:2356:91114/1‑112 (MQ=60)
GTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTG > GWNJ‑0478:712:GW2002102894th:2:2204:13076:45057/1‑150 (MQ=60)
gaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCC < GWNJ‑0478:712:GW2002102894th:2:2108:6937:89145/90‑1 (MQ=60)
GTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGG > GWNJ‑0478:712:GW2002102894th:2:2103:20746:54567/1‑150 (MQ=60)
CGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGTCCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTG < GWNJ‑0478:712:GW2002102894th:2:1206:7059:28985/150‑1 (MQ=60)
CGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAAC < GWNJ‑0478:712:GW2002102894th:2:2115:17137:43067/150‑1 (MQ=60)
TATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2101:10071:21504/1‑76 (MQ=60)
ATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgt > GWNJ‑0478:712:GW2002102894th:2:2214:9054:86766/1‑95 (MQ=60)
TGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTT > GWNJ‑0478:712:GW2002102894th:2:1205:19241:10355/1‑150 (MQ=60)
GTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCActgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaaacaaaaaaaaaaaaacgaaaata > GWNJ‑0478:712:GW2002102894th:2:2114:9193:96485/1‑46 (MQ=60)
CCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACG < GWNJ‑0478:712:GW2002102894th:2:1207:14944:52474/149‑1 (MQ=60)
GCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACGACATCCATGC < GWNJ‑0478:712:GW2002102894th:2:2206:2898:13503/150‑1 (MQ=60)
|
GCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACGACATCCATGC > AM260480/918873‑919148
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
