Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
518,612 |
G→C |
intergenic (+17/‑48) |
h16_A0492 → / → pgam2 |
Phospholipid‑binding protein/phosphoglycerate mutase 2 protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 518,612 | 0 | G | C | 100.0%
| 117.7
/ NA
| 31 | intergenic (+17/‑48) | h16_A0492/pgam2 | Phospholipid‑binding protein/phosphoglycerate mutase 2 protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (15/16); total (15/16) |
ATGGCACCTTCACCGGCGCGCAGGTGCGCGAGGCGATCCAGGGCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGGGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGCGAAACGGCCTGGAACC > AM260479/518489‑518738
|
aTGGCACCTTCACCGGCGCGCAGGTGCGCGAGGCGATCCAGGGCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCcgcg > 1:288515/1‑134 (MQ=255)
gCACCTTCACCGGCGCGCAGGTGCGCGAGGCGATCCAGGGCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCgcgg < 1:569050/134‑1 (MQ=255)
cAGGTGCGCGAGGCGATCCAGGGCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGa > 2:321868/1‑133 (MQ=255)
ggTGCGCGAGGCGATCCAGGGCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAAt < 2:581264/134‑1 (MQ=255)
cTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGcc > 2:13814/1‑134 (MQ=255)
cTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGcc > 2:25745/1‑134 (MQ=255)
cTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGcc > 1:779477/1‑134 (MQ=255)
aGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGaaa < 2:1023865/98‑1 (MQ=255)
aGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGaaa > 1:1023865/1‑98 (MQ=255)
aGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGcc > 1:411904/1‑134 (MQ=255)
ccAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCa < 2:779477/134‑1 (MQ=255)
ccAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCa < 1:74036/134‑1 (MQ=255)
cAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCAt < 1:25745/134‑1 (MQ=255)
cAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCAt < 1:991464/134‑1 (MQ=255)
aGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCa < 2:73566/132‑1 (MQ=255)
aGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCa > 1:73566/1‑132 (MQ=255)
cTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGCAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGc < 1:28954/134‑1 (MQ=255)
cACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCAtt < 1:1139616/123‑1 (MQ=255)
cACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCAtt > 2:1139616/1‑123 (MQ=255)
tGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCaa > 2:880101/1‑65 (MQ=255)
tGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCaa < 1:880101/65‑1 (MQ=255)
ccccAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGt > 1:664043/1‑87 (MQ=255)
ccccAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGt < 2:664043/87‑1 (MQ=255)
ccAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGAt < 2:1089568/134‑1 (MQ=255)
gAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGc < 2:288515/134‑1 (MQ=255)
gAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGc < 1:542120/134‑1 (MQ=255)
ggCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGc < 2:63780/87‑1 (MQ=255)
ggCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGc > 1:63780/1‑87 (MQ=255)
ggCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGCGAAACGGCCTGGAAcc > 2:144870/1‑134 (MQ=255)
ggCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGCGAAACGGCCTGGAAcc > 2:443438/1‑134 (MQ=255)
ggCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCACCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGCGAAACGGCCTGGAAcc > 1:111369/1‑134 (MQ=255)
|
ATGGCACCTTCACCGGCGCGCAGGTGCGCGAGGCGATCCAGGGCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGGGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGCGAAACGGCCTGGAACC > AM260479/518489‑518738
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GACATCGACCGGCTGCCGCTCGATGGCACCTTCACCGGCGCGCAGGTGCGCGAGGCGATCCAGGGCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGGGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGCGAAACGGCCTGGAACCGGGAGCGCCGGCTGCAGGGC > AM260479/518467‑518758
|
tactatctgttgctttttttcttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCC < GWNJ‑0478:712:GW2002102894th:2:2114:6643:38471/52‑1 (MQ=60)
ACCGGCTGCCGCTCGATGGCACCTTCACCGGCGCGCAGGTGCGCGAGGCGATCCAGGGCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGC > GWNJ‑0478:712:GW2002102894th:2:1201:21019:11987/1‑150 (MQ=60)
GGCACCTTCACCGGCGCGCAGGTGCGCGAGGCGATCCAGGGCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGG < GWNJ‑0478:712:GW2002102894th:2:1216:9116:30871/150‑1 (MQ=60)
cggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGG < GWNJ‑0478:712:GW2002102894th:2:2201:14173:66357/95‑1 (MQ=60)
ATCCAGGGCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCC > GWNJ‑0478:712:GW2002102894th:2:2113:13816:46993/1‑150 (MQ=60)
GCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCtgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:2211:15584:9852/1‑129 (MQ=60)
GCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCG > GWNJ‑0478:712:GW2002102894th:2:1208:11660:45909/1‑150 (MQ=60)
GTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTC > GWNJ‑0478:712:GW2002102894th:2:1104:12445:57144/1‑150 (MQ=60)
GCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATAC < GWNJ‑0478:712:GW2002102894th:2:1104:20314:59938/150‑1 (MQ=60)
CCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACC < GWNJ‑0478:712:GW2002102894th:2:1103:4133:64676/150‑1 (MQ=60)
CCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACC < GWNJ‑0478:712:GW2002102894th:2:2207:9989:29780/150‑1 (MQ=60)
CAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCC < GWNJ‑0478:712:GW2002102894th:2:2215:18751:88997/150‑1 (MQ=60)
CCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGCAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACC < GWNJ‑0478:712:GW2002102894th:2:1103:17290:83995/150‑1 (MQ=60)
CACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCtgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:2107:1644:8370/1‑118 (MQ=60)
GGCGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCctgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:1102:19460:98186/1‑117 (MQ=60)
CGAAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGCGAAACGGCCTGGAAC < GWNJ‑0478:712:GW2002102894th:2:1214:4935:4563/150‑1 (MQ=60)
AAATAACGTCTGGCCGGCGCCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCACCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGCGAAACGGCCTGGAACCG > GWNJ‑0478:712:GW2002102894th:2:1106:2609:77510/1‑150 (MQ=60)
CCGCGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGCGAAACGGCCTGGAACCGGGAGCGCCGGCTGCAGGGC < GWNJ‑0478:712:GW2002102894th:2:2115:8005:9074/150‑1 (MQ=60)
|
GACATCGACCGGCTGCCGCTCGATGGCACCTTCACCGGCGCGCAGGTGCGCGAGGCGATCCAGGGCCATGTGCTGGCGCAGGCCAGCCTCACCGGCACTTATACGCTGAACCCCAATCTGGCGAAATAACGTCTGGCCGGCGCCGGGCCAGCCGCGCGGCGCCGGCCTCAAAGGAAATCCCGAAGGAAACGGCATGTCGCAAAGCCCCGGGCCGCATTCGCTGGCCTATACCCACCTGATCGTGATCCGCCACGGCGAAACGGCCTGGAACCGGGAGCGCCGGCTGCAGGGC > AM260479/518467‑518758
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
