Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,191,426 |
Δ1 bp |
coding (350/390 nt) |
h16_A1095 ← |
Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,191,424 | 0 | G | . | 100.0%
| 58.9
/ NA
| 14 | coding (352/390 nt) | h16_A1095 | Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (7/7); total (7/7) |
CAGCGGCCGGGGCGGACACCTGTTCAGGCGCCGACGCCGGCAGCAGTGCGCCATCATCGATCTGCCGCAGCACGATGCTGGTGGTCAGCCCGGGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGGCCCGTGGTGTTGCCGCCTCAGCTG > AM260479/1191333‑1191546
|
cAGCGGCCGGGGCGGACACCTGTTCAGGCGCCGACGCCGGCAGCAGTGCGCCATCATCGATCTGCCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGccac > 2:1077738/1‑134 (MQ=255)
ggggCGGACACCTGTTCAGGCGCCGACGCCGGCAGCAGTGCGCCATCATCGATCTGCCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCAccgccg < 2:524273/134‑1 (MQ=255)
ttCAGGCGCCGACGCCGGCAGCAGTGCGCCATCATCGATCTGCCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCgcgcgcggcg < 2:341171/133‑1 (MQ=255)
cagcagTGCGCCATCATCGATCTGCCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGAttgcg < 1:1158088/134‑1 (MQ=255)
tCTGCCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCcga < 1:110397/63‑1 (MQ=255)
tCTGCCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCcga > 2:110397/1‑63 (MQ=255)
cagcaCGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGGCCCGTGGTGTTg > 2:202111/1‑134 (MQ=255)
agatcGATGATGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGGCCCGTGGTGTTgc > 1:136459/5‑134 (MQ=255)
cGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGGCCCGTGGTGTTgccgcc < 1:1077738/134‑1 (MQ=255)
tggtggTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCgcgcgcggcg > 1:1136525/1‑77 (MQ=255)
tggtggTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCgcgcgcggcg < 2:1136525/77‑1 (MQ=255)
ggtggtCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGGCCCGTGGTGTTGCCGCCTCAGCtg > 1:353366/1‑134 (MQ=255)
tCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCcgcgcg > 1:942644/1‑62 (MQ=255)
tCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCcgcgcg < 2:942644/62‑1 (MQ=255)
|
CAGCGGCCGGGGCGGACACCTGTTCAGGCGCCGACGCCGGCAGCAGTGCGCCATCATCGATCTGCCGCAGCACGATGCTGGTGGTCAGCCCGGGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGGCCCGTGGTGTTGCCGCCTCAGCTG > AM260479/1191333‑1191546
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCGGGCGCTGCCGCCATCTCTGCGGCGGGCGCAGCGGCCGGGGCGGACACCTGTTCAGGCGCCGACGCCGGCAGCAGTGCGCCATCATCGATCTGCCGCAGCACGATGCTGGTGGTCAGCCCGGGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGGCCCGTGGTGTTGCCGCCTCAGCTGCCTGTGCCTGCAGC > AM260479/1191302‑1191560
|
tcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaggcggcagatcgatgatggcgcactgCTGCCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAG < GWNJ‑0478:712:GW2002102894th:2:2214:6205:65817/57‑1 (MQ=60)
gacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGTGCGCCATCATCGATCTGCCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACC < GWNJ‑0478:712:GW2002102894th:2:1106:17084:71831/93‑1 (MQ=60)
ttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaggcccGGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGG < GWNJ‑0478:712:GW2002102894th:2:1201:2882:81093/77‑1 (MQ=60)
ggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTG < GWNJ‑0478:712:GW2002102894th:2:1213:3337:29446/96‑1 (MQ=60)
gggcgcagcggccgggctgaccaccagcatcgtgctgcggcagatCGATGCGGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGCGCGAGCGCCAGCACCGCCGCGCGCGCCCGGCGCGCGCTGCCATGGATTGCGTTG > GWNJ‑0478:712:GW2002102894th:2:2215:12084:68952/46‑150 (MQ=60)
GCAGCAGTGCGCCATCATCGATCTGCCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGG < GWNJ‑0478:712:GW2002102894th:2:2216:12962:74792/150‑1 (MQ=60)
gcatcgtgctgcggcagatCGATGATGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGGCCCGTGGTGTTGCC > GWNJ‑0478:712:GW2002102894th:2:1109:13040:19956/20‑150 (MQ=60)
GCCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATctgtctcttatacacatctccgagcccacgagacactcgcta > GWNJ‑0478:712:GW2002102894th:2:2215:3639:75032/1‑107 (MQ=60)
CCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGccccggtcggtgccgctgccagcgcg > GWNJ‑0478:712:GW2002102894th:2:2212:7725:91736/1‑124 (MQ=60)
CCGCAGCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGGCCCGTGGTGTTGCCGCCTCAGCTGC > GWNJ‑0478:712:GW2002102894th:2:1204:13680:10553/1‑150 (MQ=60)
GCACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCtgccatggatctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtct > GWNJ‑0478:712:GW2002102894th:2:2205:6861:50546/1‑82 (MQ=60)
ACGATGCTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGGCCCGTGGTGTTGCCGCCTCAGCTGCCTGTGCC < GWNJ‑0478:712:GW2002102894th:2:2212:17840:90306/150‑1 (MQ=60)
CTGGTGGTCAGCCC‑GGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGctgtctcttatacacatctccgagcccacgagacactcgctaatctc > GWNJ‑0478:712:GW2002102894th:2:1207:11712:39920/1‑103 (MQ=60)
|
GCGGGCGCTGCCGCCATCTCTGCGGCGGGCGCAGCGGCCGGGGCGGACACCTGTTCAGGCGCCGACGCCGGCAGCAGTGCGCCATCATCGATCTGCCGCAGCACGATGCTGGTGGTCAGCCCGGGCCACTGCCGCGACGCTGGCCACTAGCCCGAGCGAGCGCCACCACCGCCGCGCGCGCGCGGCGCGCGCTGCCATGGATTGCGTTGCGGTCCCTGTGGCCCGTGGTGTTGCCGCCTCAGCTGCCTGTGCCTGCAGC > AM260479/1191302‑1191560
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
