Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,579,243 |
A→G |
I111T (ATT→ACT) |
h16_A2374 ← |
Metallopeptidase, M23B subfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,579,243 | 0 | A | G | 100.0%
| 51.9
/ NA
| 15 | I111T (ATT→ACT) | h16_A2374 | Metallopeptidase, M23B subfamily |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (7/8); total (7/8) |
GGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATT > AM260479/2579113‑2579365
|
ggcgggcgTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAgtgt > 2:606863/1‑134 (MQ=255)
cggccgGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCgcag > 1:455223/1‑133 (MQ=255)
gcCTGGTCGATAGGGTGCGACTGCAACGTGCCCGGCGCCTCCGGCATGGGGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCAACCTCGATCTGGTTCACATTGGt < 2:515293/134‑1 (MQ=255)
aTCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCcggc > 1:334752/1‑78 (MQ=255)
aTCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCcggc < 2:334752/78‑1 (MQ=255)
cTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACAtt > 1:427343/1‑117 (MQ=255)
cTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACAtt < 2:427343/117‑1 (MQ=255)
ccTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGcc < 1:606863/134‑1 (MQ=255)
cACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACAt < 2:517920/134‑1 (MQ=255)
aCCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATc > 2:528026/1‑134 (MQ=255)
ccGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATAc > 2:505392/1‑134 (MQ=255)
cGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCg < 2:771394/79‑1 (MQ=255)
gCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCt < 1:925982/78‑1 (MQ=255)
gCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCt > 2:925982/1‑78 (MQ=255)
gCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAgcg < 1:505392/133‑1 (MQ=255)
cGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGAtt > 2:725441/1‑134 (MQ=255)
|
GGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATT > AM260479/2579113‑2579365
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGGT > AM260479/2579103‑2579376
|
tagcgagtgtctcgtgggctcggagatgtgtataagagacaGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACG < GWNJ‑0478:712:GW2002102894th:2:2106:20757:17188/108‑1 (MQ=60)
GCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGC > GWNJ‑0478:712:GW2002102894th:2:1211:9586:77420/1‑149 (MQ=60)
agcgagtgtctcgtgggctcggagatgtgtataagagacaggtgcaggcGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCA < GWNJ‑0478:712:GW2002102894th:2:2204:4111:50985/100‑1 (MQ=60)
GGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCctttctcttatacacatctccgagcccacgagacactcgct > GWNJ‑0478:712:GW2002102894th:2:1202:4631:99587/1‑108 (MQ=60)
GGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACtg > GWNJ‑0478:712:GW2002102894th:2:1209:17046:31063/1‑148 (MQ=60)
CGCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCctgtctcttatacacatctccgagcccacgagacactcgct > GWNJ‑0478:712:GW2002102894th:2:2113:8584:6121/1‑109 (MQ=60)
GCCTGCACCGTGCCCGGCGCCACCGGCATGGTGGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGAC < GWNJ‑0478:712:GW2002102894th:2:2103:9781:52413/150‑1 (MQ=60)
tcgtgcCGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGG > GWNJ‑0478:712:GW2002102894th:2:2104:19009:76635/7‑150 (MQ=60)
GGCCACGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGG < GWNJ‑0478:712:GW2002102894th:2:1212:6202:28938/149‑1 (MQ=60)
gtgcCGCCGGGCGCAGTGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGGT > GWNJ‑0478:712:GW2002102894th:2:2206:6139:60333/5‑149 (MQ=60)
|
CGGCGGGCGCGGCGGGCGTTGCCGCAGGCGCCGAAGCGGCCGCGGCCGGAGGCACCGGCGTGGCGGCCGGGCGGGCCTGGTCGATCGGCTGCGCCTGCACCGTGCCCGGGGCCACCGGCATGGTGGCCACGCCGGGCGCAATGTTGACGTCGGCACCCGGCGGCACGATGCGCAGCAGCTGGCCGACCTCGATCTGGTTCACATTGGTCAGGTTGTTCCACGTTGCCACATCGCGATACGACTGGCCGTTCTCCAGCGCGATTCGGTAAAGGGT > AM260479/2579103‑2579376
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
