Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,580,039 |
A→G |
R155R (CGT→CGC) |
h16_A2375 ← |
Protein‑L‑isoaspartate carboxylmethyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,580,039 | 0 | A | G | 100.0%
| 53.2
/ NA
| 15 | R155R (CGT→CGC) | h16_A2375 | Protein‑L‑isoaspartate carboxylmethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (8/7); total (8/7) |
TGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCACGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTGGCGC > AM260479/2579911‑2580162
|
tGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCgcag > 1:192409/1‑134 (MQ=255)
tGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCgcag > 1:286613/1‑134 (MQ=255)
cgcgcgACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGAt > 1:175659/1‑107 (MQ=255)
cgcgcgACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGAt < 2:175659/107‑1 (MQ=255)
ggCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATc > 1:732591/1‑133 (MQ=255)
ggCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATc < 2:732591/133‑1 (MQ=255)
cTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCgg > 1:223918/1‑132 (MQ=255)
gTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAgcg < 2:192409/134‑1 (MQ=255)
ggcgCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGcgcg > 1:1108040/1‑96 (MQ=255)
ggcgCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGcgcg < 2:1108040/96‑1 (MQ=255)
tCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCg > 1:818760/1‑49 (MQ=255)
tCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCg < 2:818760/49‑1 (MQ=255)
ggcggcCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGccacca < 1:816940/44‑1 (MQ=255)
ggcggcCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGccacca > 2:816940/1‑44 (MQ=255)
ccAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTGgcgc < 2:223918/134‑1 (MQ=255)
|
TGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCACGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTGGCGC > AM260479/2579911‑2580162
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCCTTGGCCTGCTCGTGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCACGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGT > AM260479/2579896‑2580157
|
GCCTTGGCCTGCTCGTGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGC > GWNJ‑0478:712:GW2002102894th:2:1113:2680:19893/1‑150 (MQ=60)
GCCTTGGCCTGCTCGTGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGC > GWNJ‑0478:712:GW2002102894th:2:1116:6509:99081/1‑150 (MQ=60)
GGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCActgtctcttat > GWNJ‑0478:712:GW2002102894th:2:2201:10741:73417/1‑139 (MQ=60)
ATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCtgtctcttatac > GWNJ‑0478:712:GW2002102894th:2:1110:8932:30346/1‑138 (MQ=60)
tttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAA < GWNJ‑0478:712:GW2002102894th:2:2115:11266:38601/75‑1 (MQ=60)
CTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGG > GWNJ‑0478:712:GW2002102894th:2:2109:4017:99786/1‑150 (MQ=60)
GCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGC > GWNJ‑0478:712:GW2002102894th:2:1112:15009:80210/1‑148 (MQ=60)
AGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCctgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:2214:11013:39082/1‑126 (MQ=60)
CGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaa > GWNJ‑0478:712:GW2002102894th:2:2115:3084:47310/1‑79 (MQ=60)
CTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCctgtctcttatacacatctacgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaagatacaaagaaaaagaaaccaccgcgtg > GWNJ‑0478:712:GW2002102894th:2:2206:8659:88101/1‑42 (MQ=60)
|
GCCTTGGCCTGCTCGTGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCACGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGT > AM260479/2579896‑2580157
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
