Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,302,920 |
+C |
coding (1199/1251 nt) |
dfp → |
phosphopantothenoylcysteine synthetase/decarboxylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,302,918 | 1 | . | C | 100.0%
| 54.2
/ NA
| 15 | R399R (CGG→CGC) | dfp | phosphopantothenoylcysteine synthetase/decarboxylase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (6/9); total (6/9) |
TTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGG‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTC > AM260479/3302817‑3303036
|
ttCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATg < 2:796365/133‑1 (MQ=255)
tCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATgcg > 2:891169/1‑134 (MQ=255)
gACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATgcgc < 1:902109/133‑1 (MQ=255)
aCGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCgg > 1:1049859/1‑134 (MQ=255)
cATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTa > 2:272576/1‑134 (MQ=255)
gcgGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGt < 2:210863/134‑1 (MQ=255)
ctggcgcgcCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAAcc < 1:495763/131‑1 (MQ=255)
tGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGa > 2:420781/1‑134 (MQ=255)
gcgcgcCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGa < 1:891169/132‑1 (MQ=255)
gtggCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAAc < 1:281736/134‑1 (MQ=255)
cccGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTc < 1:420781/134‑1 (MQ=255)
gggCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGt > 1:217785/1‑64 (MQ=255)
gggCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGt < 2:217785/64‑1 (MQ=255)
ggCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCTAGGATTCCCATGAc < 1:473317/93‑1 (MQ=255)
ggCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCTAGGATTCCCATGAc > 2:473317/1‑93 (MQ=255)
|
TTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGG‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTC > AM260479/3302817‑3303036
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CACCATACCTTCGGCCTGGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GG‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCCTGAATGAATGGGGCCTGCCCG > AM260479/3302778‑3303067
|
cggagatgtgtataagagacaGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGG < GWNJ‑0478:712:GW2002102894th:2:1106:19925:94588/129‑1 (MQ=60)
CACCATACCTTCGGCCTGGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGG < GWNJ‑0478:712:GW2002102894th:2:2114:14445:16029/150‑1 (MQ=60)
CACCATACCTTCGGCCTGGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GG‑CCctgatgc > GWNJ‑0478:712:GW2002102894th:2:2215:4384:69521/1‑143 (MQ=60)
cggagatgtgtataagagacagCAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGTGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGG < GWNJ‑0478:712:GW2002102894th:2:2111:2946:27150/127‑1 (MQ=60)
ATACCTTCGGCCTGGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACAGC‑CCTGATGGCGGG < GWNJ‑0478:712:GW2002102894th:2:2113:18597:25515/150‑1 (MQ=60)
ATACCTTCGGCCTGGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGG < GWNJ‑0478:712:GW2002102894th:2:2114:9314:90254/150‑1 (MQ=60)
AAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGC > GWNJ‑0478:712:GW2002102894th:2:2210:12247:45883/1‑150 (MQ=60)
CGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTC < GWNJ‑0478:712:GW2002102894th:2:2202:9665:4043/149‑1 (MQ=60)
CCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GG‑CCcctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatg > GWNJ‑0478:712:GW2002102894th:2:1108:13421:49691/1‑97 (MQ=60)
GCTG‑‑GCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGAT < GWNJ‑0478:712:GW2002102894th:2:1213:20976:80597/150‑1 (MQ=60)
gggctcggagatgtgtataagagacaGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCTAGGATTCCCATGACCCAGCCACTGAACCC < GWNJ‑0478:712:GW2002102894th:2:1210:14773:70460/124‑1 (MQ=60)
GGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACC < GWNJ‑0478:712:GW2002102894th:2:2203:14532:36330/148‑1 (MQ=60)
GGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATC < GWNJ‑0478:712:GW2002102894th:2:1116:5696:70389/150‑1 (MQ=60)
GGCCAGCGCCTGCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgt > GWNJ‑0478:712:GW2002102894th:2:1112:8398:49708/1‑95 (MQ=60)
GCCCGGGCGCACAC‑GGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCCTGAAT < GWNJ‑0478:712:GW2002102894th:2:1208:10605:95723/150‑1 (MQ=60)
GC‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCCTGAATGAATGGGGCCTGCCC > GWNJ‑0478:712:GW2002102894th:2:1207:3162:7460/1‑150 (MQ=60)
C‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccg > GWNJ‑0478:712:GW2002102894th:2:2109:20960:53101/1‑96 (MQ=60)
|
CACCATACCTTCGGCCTGGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACAC‑GG‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCCTGAATGAATGGGGCCTGCCCG > AM260479/3302778‑3303067
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
