Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
963,917 |
Δ1 bp |
intergenic (+84/‑3) |
h16_B0849 → / → h16_B0850 |
Acyl‑CoA dehydrogenase/Acyl‑CoA dehydrogenase, short‑chain specific |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 963,914 | 0 | A | . | 100.0%
| 100.1
/ NA
| 23 | intergenic (+81/‑6) | h16_B0849/h16_B0850 | Acyl‑CoA dehydrogenase/Acyl‑CoA dehydrogenase, short‑chain specific |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (12/11); total (12/11) |
AGCACGAACTGCTGGCCAAGCAGGTGCTGGGCCTGTAGTGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGGAAAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGGGCTGGCTGGCCGTG > AM260480/963796‑964039
|
aGCACGAACTGCTGGCCAAGCAGGTGCTGGGCCTGTAGTGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTa > 1:463386/1‑134 (MQ=255)
ctgGCCAAGCAGGTGCTGGGCCTGTAGTGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGTTGCAGG‑AAAGCGTGCGGCGCTATGTCGAAcgcg < 2:391596/134‑1 (MQ=255)
tGTAGTGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGcgccg > 1:297461/1‑134 (MQ=255)
tGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTAc > 2:468567/1‑112 (MQ=255)
tGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTAc < 1:468567/112‑1 (MQ=255)
tGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCgcgg > 2:603046/1‑134 (MQ=255)
cAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTgg > 2:1141564/1‑134 (MQ=255)
cAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTgc < 1:560218/134‑2 (MQ=255)
cGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGa > 2:843718/1‑134 (MQ=255)
aGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTtctc > 1:1039505/1‑129 (MQ=255)
aGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTtctc < 2:1039505/129‑1 (MQ=255)
cTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTgc < 1:87557/105‑2 (MQ=255)
cTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTgc > 2:87557/1‑104 (MQ=255)
cTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCAc > 2:214239/1‑134 (MQ=255)
cacaTGAGCGATCCAACGGACGATCGCCGCATGCTGCAGGaaag > 1:503173/1‑43 (MQ=255)
cacaTGAGCGATCCAACGGACGATCGCCGCATGCTGCAGGaaag < 2:503173/44‑2 (MQ=255)
cacaTGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAgcgc < 2:418741/87‑1 (MQ=255)
cacaTGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAgcgc > 1:418741/1‑87 (MQ=255)
cacaTGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTgg > 1:773950/1‑132 (MQ=255)
cGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGggct < 1:1141564/134‑1 (MQ=255)
gccgcATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAgcgc < 1:926868/62‑1 (MQ=255)
gccgcATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAgcgc > 2:926868/1‑62 (MQ=255)
tgctgcAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGGGCTGGCTGGCCGTg < 2:297461/134‑1 (MQ=255)
tgctgcAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGGGCTGGCTGGCCGTg < 2:773950/134‑1 (MQ=255)
tgctgcAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGGGCTGGCTGGCCGTg < 1:843718/134‑1 (MQ=255)
|
AGCACGAACTGCTGGCCAAGCAGGTGCTGGGCCTGTAGTGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGGAAAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGGGCTGGCTGGCCGTG > AM260480/963796‑964039
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCTCCAGCGAGATCCAGCACGAACTGCTGGCCAAGCAGGTGCTGGGCCTGTAGTGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGGAAAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGGGCTGGCTGGCCGTGGGGCTGCCCGAAGCCTGC > AM260480/963781‑964057
|
GCTCCAGCGAGATCCAGCACGAACTGCTGGCCAAGCAGGTGCTGGGCCTGTAGTGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTAT > GWNJ‑0478:712:GW2002102894th:2:1210:15573:18866/1‑150 (MQ=60)
agagaCAGGTGCTGGGCCTGTAGTGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGC < GWNJ‑0478:712:GW2002102894th:2:1210:11381:46000/145‑1 (MQ=60)
AGCAGGTGCTGGGCCTGTAGTGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGC > GWNJ‑0478:712:GW2002102894th:2:1201:12750:68725/1‑150 (MQ=60)
agacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCC < GWNJ‑0478:712:GW2002102894th:2:2204:2000:56445/93‑1 (MQ=60)
gtgtataagagacagACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGctcgaagccgtagccg < GWNJ‑0478:712:GW2002102894th:2:1107:13003:39920/135‑17 (MQ=60)
CCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGctcgaagccgtagccg < GWNJ‑0478:712:GW2002102894th:2:1214:5741:88661/150‑17 (MQ=60)
CCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCA > GWNJ‑0478:712:GW2002102894th:2:2211:12538:90600/1‑149 (MQ=60)
ACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGctcgaagccgtagccgcgttcgacatagcgc < GWNJ‑0478:712:GW2002102894th:2:2103:12194:28579/150‑32 (MQ=60)
GGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1212:15103:16996/1‑75 (MQ=60)
GGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCctgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:1208:14133:83923/1‑117 (MQ=60)
GGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGC > GWNJ‑0478:712:GW2002102894th:2:2113:19491:19093/1‑148 (MQ=60)
ACGATCGCCGCATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGGGCTGGCTGGCCGTGGGGC < GWNJ‑0478:712:GW2002102894th:2:2215:4205:97581/150‑1 (MQ=60)
ATGCTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGGGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGGGCTGGCTGGCCGTGGGGCTGCCCGAAGCC < GWNJ‑0478:712:GW2002102894th:2:2114:2631:69696/150‑1 (MQ=60)
CTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGGGCTGGCTGGCCGTGGGGCTGCCCGAAGCCctg > GWNJ‑0478:712:GW2002102894th:2:2103:16273:89598/1‑147 (MQ=60)
CTGCAGG‑AAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGGGCTGGCTGGCCGTGGGGCTGCCCGAAGCCTGC > GWNJ‑0478:712:GW2002102894th:2:2210:21217:56494/1‑150 (MQ=60)
|
GCTCCAGCGAGATCCAGCACGAACTGCTGGCCAAGCAGGTGCTGGGCCTGTAGTGGCTCCAATCGTGCCCGCAACCCAGGACAAGGAACTGAACACATGAGCGATCCAACGGACGATCGCCGCATGCTGCAGGAAAAGCGTGCGGCGCTATGTCGAACGCGGCTACGGCTTCGAGCAGCGCCGCGCGGCGCTGGCCGAGCCCGATGGCTTCTCGCGCAAGCACTGGCAGGCGTTTGCCGAAATGGGCTGGCTGGCCGTGGGGCTGCCCGAAGCCTGC > AM260480/963781‑964057
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
