breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451699,7220AG61.8% 6.6 / 32.6 34intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*AE0154514,974,4390G.51.8% 41.4 / 65.6 27coding (1165/2043 nt)flgKFlagellar hook‑associated protein FlgK
*AE0154514,974,4380A.48.3% 54.1 / 54.3 29coding (1166/2043 nt)flgKFlagellar hook‑associated protein FlgK
*AE0154511,327,1210CT47.5% 32.6 / 90.6 61intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*AE0154514,348,7050AG46.9% 10.0 / 43.6 32intergenic (‑70/+404)PP_3820/galUGroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*AE0154514,964,4540CT44.0% 1.8 / 28.8 25D302N (GAC→AAC) fleQTranscriptional regulator FleQ
*AE0154511,843,9010CT36.0% 20.8 / 23.2 25R298H (CGC→CAC) gacSSensor protein GacS
*AE0154515,988,8090GA35.3% 9.5 / 13.5 17intergenic (+103/‑443)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*AE0154511,530,1730AC33.3% 20.1 / 15.7 18*399S (TAA→TCA) 
noncoding (62/244 nt)
ftsZ
PP_mr19
Cell division protein FtsZ
Xoo8 ncRNA
*AE0154514,583,5710G.30.8% 24.1 / 10.2 13intergenic (‑144/+27)treSB/glgEfused trehalose synthase B/maltokinase/Alpha‑1,4‑glucan:maltose‑1‑phosphate maltosyltransferase
*AE0154515,988,9110A.22.2% 48.1 / 10.2 18intergenic (+205/‑341)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*AE015451197,5720GA20.0% 58.9 / 11.2 30K1026K (AAG→AAAPP_0168putative surface adhesion protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451 = 247211518 (0.800)3 (0.140) 3/302 2.6 16.1% intergenic (+19/+130) PP_2166/PP_2167 Anti‑anti‑sigma factor/putative PhoD‑like phosphatase
?AE015451 2472313 = 14 (0.650)coding (1783/1851 nt) PP_2167 putative PhoD‑like phosphatase