breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG44.2% 373.4 / 76.1 252intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123063,938,8870CT37.4% 277.0 / 169.5 206S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123061,944,2880AC33.5% 405.9 / 53.3 210G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,527,3680AC33.0% 529.7 / 79.5 265L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306341,3770TG31.3% 188.4 / 25.8 115G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP012306935,0540GT30.3% 195.0 / 36.4 76intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP012306644,4170GA28.7% 927.5 / inf 537intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP012306406,6770AG28.4% 471.8 / 30.8 205K294E (AAA→GAA) hemHferrochelatase
*AP0123061,226,5570AG27.8% 371.7 / 82.8 180G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123061,854,8510AC26.9% 679.8 / 78.6 294T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP012306800,6750AC25.9% 609.8 / 23.8 255T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123062,558,0710AC25.1% 585.1 / 36.6 243V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP0123061,393,3790AC23.7% 752.9 / 40.4 281T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase
*AP0123063,182,8610AC23.5% 302.6 / 15.9 119L198F (TTA→TTCyiaNpredicted transporter
*AP0123062,758,9090TG23.3% 618.3 / 13.5 232G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123063,903,7910AG23.0% 599.9 / 60.4 248E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123061,128,4120TG22.7% 705.7 / 27.5 274V29G (GTG→GGG) yciOconserved protein
*AP0123062,857,9110AC22.2% 523.8 / 12.5 189K180N (AAA→AACaaeRpredicted DNA‑binding transcriptional regulator, efflux system
*AP012306202,4170TG21.9% 698.6 / 19.0 282V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123063,412,0490TG21.9% 822.4 / 36.4 335intergenic (+25/‑122)proM/aslBtRNA‑Pro/predicted regulator of arylsulfatase activity

Marginal new junction evidence (lowest skew 10 of 233 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 2275012246 (0.810)11 (0.040) 9/274 15.4 4.6% noncoding (60/76 nt) gltW tRNA‑Glu
?AP012306 3372719 = NA (NA)noncoding (72/76 nt) gltU tRNA‑Glu
* ? AP012306 = 3014868342 (1.120)8 (0.030) 8/274 15.8 2.5% coding (930/1506 nt) glpD sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
?AP012306 = 3014856 317 (1.130)coding (918/1506 nt) glpD sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
* ? AP012306 215967 =NA (NA)9 (0.030) 9/294 15.8 NA noncoding (1348/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 215993 = NA (NA)noncoding (1374/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 236403316 (1.040)10 (0.040) 7/258 15.9 3.8% intergenic (+80/‑96) yafL/yafM predicted lipoprotein and C40 family peptidase/conserved protein
?AP012306 237045 = 234 (0.890)intergenic (+49/‑2715) yafM/dinB conserved protein/DNA polymerase IV
* ? AP012306 3597644 =233 (0.770)8 (0.030) 7/274 16.3 3.6% noncoding (60/76 nt) gltT tRNA‑Glu
?AP012306 3639058 = NA (NA)noncoding (72/76 nt) gltV tRNA‑Glu
* ? AP012306 2817012 =329 (1.080)7 (0.020) 7/286 16.5 2.1% coding (300/558 nt) yhbN predicted transporter subunit
?AP012306 2817029 = 329 (1.130)coding (317/558 nt) yhbN predicted transporter subunit
* ? AP012306 1439147 =280 (0.920)7 (0.020) 7/284 16.5 2.5% coding (144/237 nt) lpp murein lipoprotein
?AP012306 1439162 = 276 (0.950)coding (159/237 nt) lpp murein lipoprotein
* ? AP012306 3772678 =383 (1.260)7 (0.020) 7/288 16.6 1.9% coding (196/1410 nt) melB melibiose:sodium symporter
?AP012306 3772718 = 370 (1.260)coding (236/1410 nt) melB melibiose:sodium symporter
* ? AP012306 = 3765699284 (0.930)8 (0.030) 7/290 16.6 2.8% coding (435/1338 nt) adiC arginine:agmatin
?AP012306 = 3765710 286 (0.970)coding (424/1338 nt) adiC arginine:agmatin
* ? AP012306 2943459 =356 (1.170)7 (0.020) 7/290 16.6 2.0% coding (88/168 nt) yhfG predicted protein
?AP012306 2943475 = 348 (1.170)coding (72/168 nt) yhfG predicted protein