breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 44.2% | 373.4 / 76.1 | 252 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 3,938,887 | 0 | C | T | 37.4% | 277.0 / 169.5 | 206 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 1,944,288 | 0 | A | C | 33.5% | 405.9 / 53.3 | 210 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 3,527,368 | 0 | A | C | 33.0% | 529.7 / 79.5 | 265 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 341,377 | 0 | T | G | 31.3% | 188.4 / 25.8 | 115 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 935,054 | 0 | G | T | 30.3% | 195.0 / 36.4 | 76 | intergenic (+24/‑1406) | putP/ycdO | proline:sodium symporter/conserved protein |
* | AP012306 | 644,417 | 0 | G | A | 28.7% | 927.5 / inf | 537 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 406,677 | 0 | A | G | 28.4% | 471.8 / 30.8 | 205 | K294E (AAA→GAA) | hemH | ferrochelatase |
* | AP012306 | 1,226,557 | 0 | A | G | 27.8% | 371.7 / 82.8 | 180 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 1,854,851 | 0 | A | C | 26.9% | 679.8 / 78.6 | 294 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 800,675 | 0 | A | C | 25.9% | 609.8 / 23.8 | 255 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 2,558,071 | 0 | A | C | 25.1% | 585.1 / 36.6 | 243 | V302G (GTA→GGA) | visC | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
* | AP012306 | 1,393,379 | 0 | A | C | 23.7% | 752.9 / 40.4 | 281 | T92P (ACC→CCC) | nth | DNA glycosylase and apyrimidinic (AP) lyase |
* | AP012306 | 3,182,861 | 0 | A | C | 23.5% | 302.6 / 15.9 | 119 | L198F (TTA→TTC) | yiaN | predicted transporter |
* | AP012306 | 2,758,909 | 0 | T | G | 23.3% | 618.3 / 13.5 | 232 | G33G (GGT→GGG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
* | AP012306 | 3,903,791 | 0 | A | G | 23.0% | 599.9 / 60.4 | 248 | E189G (GAA→GGA) | yjgJ | predicted transcriptional regulator |
* | AP012306 | 1,128,412 | 0 | T | G | 22.7% | 705.7 / 27.5 | 274 | V29G (GTG→GGG) | yciO | conserved protein |
* | AP012306 | 2,857,911 | 0 | A | C | 22.2% | 523.8 / 12.5 | 189 | K180N (AAA→AAC) | aaeR | predicted DNA‑binding transcriptional regulator, efflux system |
* | AP012306 | 202,417 | 0 | T | G | 21.9% | 698.6 / 19.0 | 282 | V409G (GTA→GGA) | tilS | tRNA(Ile)‑lysidine synthetase |
* | AP012306 | 3,412,049 | 0 | T | G | 21.9% | 822.4 / 36.4 | 335 | intergenic (+25/‑122) | proM/aslB | tRNA‑Pro/predicted regulator of arylsulfatase activity |
Marginal new junction evidence (lowest skew 10 of 233 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | = 2275012 | 246 (0.810) | 11 (0.040) | 9/274 | 15.4 | 4.6% | noncoding (60/76 nt) | gltW | tRNA‑Glu |
? | AP012306 | 3372719 = | NA (NA) | noncoding (72/76 nt) | gltU | tRNA‑Glu | |||||
* | ? | AP012306 | = 3014868 | 342 (1.120) | 8 (0.030) | 8/274 | 15.8 | 2.5% | coding (930/1506 nt) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
? | AP012306 | = 3014856 | 317 (1.130) | coding (918/1506 nt) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding | |||||
* | ? | AP012306 | 215967 = | NA (NA) | 9 (0.030) | 9/294 | 15.8 | NA | noncoding (1348/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) |
? | AP012306 | 215993 = | NA (NA) | noncoding (1374/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) | |||||
* | ? | AP012306 | = 236403 | 316 (1.040) | 10 (0.040) | 7/258 | 15.9 | 3.8% | intergenic (+80/‑96) | yafL/yafM | predicted lipoprotein and C40 family peptidase/conserved protein |
? | AP012306 | 237045 = | 234 (0.890) | intergenic (+49/‑2715) | yafM/dinB | conserved protein/DNA polymerase IV | |||||
* | ? | AP012306 | 3597644 = | 233 (0.770) | 8 (0.030) | 7/274 | 16.3 | 3.6% | noncoding (60/76 nt) | gltT | tRNA‑Glu |
? | AP012306 | 3639058 = | NA (NA) | noncoding (72/76 nt) | gltV | tRNA‑Glu | |||||
* | ? | AP012306 | 2817012 = | 329 (1.080) | 7 (0.020) | 7/286 | 16.5 | 2.1% | coding (300/558 nt) | yhbN | predicted transporter subunit |
? | AP012306 | 2817029 = | 329 (1.130) | coding (317/558 nt) | yhbN | predicted transporter subunit | |||||
* | ? | AP012306 | 1439147 = | 280 (0.920) | 7 (0.020) | 7/284 | 16.5 | 2.5% | coding (144/237 nt) | lpp | murein lipoprotein |
? | AP012306 | 1439162 = | 276 (0.950) | coding (159/237 nt) | lpp | murein lipoprotein | |||||
* | ? | AP012306 | 3772678 = | 383 (1.260) | 7 (0.020) | 7/288 | 16.6 | 1.9% | coding (196/1410 nt) | melB | melibiose:sodium symporter |
? | AP012306 | 3772718 = | 370 (1.260) | coding (236/1410 nt) | melB | melibiose:sodium symporter | |||||
* | ? | AP012306 | = 3765699 | 284 (0.930) | 8 (0.030) | 7/290 | 16.6 | 2.8% | coding (435/1338 nt) | adiC | arginine:agmatin |
? | AP012306 | = 3765710 | 286 (0.970) | coding (424/1338 nt) | adiC | arginine:agmatin | |||||
* | ? | AP012306 | 2943459 = | 356 (1.170) | 7 (0.020) | 7/290 | 16.6 | 2.0% | coding (88/168 nt) | yhfG | predicted protein |
? | AP012306 | 2943475 = | 348 (1.170) | coding (72/168 nt) | yhfG | predicted protein |