breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 30 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 45.1% | 399.0 / 81.1 | 273 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 1,944,288 | 0 | A | C | 38.3% | 343.3 / 45.6 | 188 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 3,527,368 | 0 | A | C | 32.0% | 588.5 / 73.7 | 275 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 644,417 | 0 | G | A | 31.6% | 853.5 / inf | 593 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 341,377 | 0 | T | G | 31.1% | 203.9 / 24.3 | 124 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 2,758,909 | 0 | T | G | 31.1% | 539.4 / 33.5 | 235 | G33G (GGT→GGG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
* | AP012306 | 800,675 | 0 | A | C | 30.3% | 565.3 / 44.6 | 254 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 3,590,367 | 0 | A | C | 28.2% | 663.8 / 40.4 | 267 | T30P (ACC→CCC) | fabR | DNA‑binding transcriptional repressor |
* | AP012306 | 2,714,555 | 0 | T | G | 27.2% | 656.1 / 24.4 | 285 | T175P (ACC→CCC) | ygjV | conserved inner membrane protein |
* | AP012306 | 257,137 | 0 | T | G | 26.3% | 379.4 / 10.1 | 179 | L496V (TTG→GTG) | betT | choline transporter of high affinity |
* | AP012306 | 3,903,791 | 0 | A | G | 26.0% | 616.9 / 72.5 | 266 | E189G (GAA→GGA) | yjgJ | predicted transcriptional regulator |
* | AP012306 | 1,066,323 | 0 | A | C | 25.8% | 715.6 / 23.2 | 271 | intergenic (‑92/+33) | ychM/prsA | predicted transporter/phosphoribosylpyrophosphate synthase |
* | AP012306 | 1,854,851 | 0 | A | C | 25.5% | 765.3 / 59.5 | 291 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 3,938,887 | 0 | C | T | 25.1% | 580.9 / 138.2 | 267 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 3,639,040 | 0 | T | G | 24.7% | 166.4 / 15.4 | 77 | noncoding (54/76 nt) | gltV | tRNA‑Glu |
* | AP012306 | 2,212,753 | 0 | A | C | 24.1% | 343.2 / 41.8 | 133 | Y241D (TAT→GAT) | hcaT | predicted 3‑phenylpropionic transporter |
* | AP012306 | 3,282,076 | 0 | T | G | 23.8% | 564.3 / 15.2 | 244 | intergenic (‑79/+27) | ilvB/ivbL | acetolactate synthase I, large subunit/ilvB operon leader peptide |
* | AP012306 | 202,417 | 0 | T | G | 23.5% | 676.4 / 27.7 | 278 | V409G (GTA→GGA) | tilS | tRNA(Ile)‑lysidine synthetase |
* | AP012306 | 1,074,619 | 0 | T | C | 23.2% | 364.2 / 99.3 | 194 | S29G (AGC→GGC) | ldrA | toxic polypeptide, small |
* | AP012306 | 3,645,642 | 0 | A | C | 23.0% | 832.0 / 30.0 | 313 | T318P (ACC→CCC) | aceB | malate synthase A |
Marginal new junction evidence (lowest skew 10 of 143 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | = 1092512 | 382 (1.190) | 25 (0.090) | 15/258 | 9.4 | 7.4% | intergenic (+100/+3) | ychS/tpr | predicted protein/predicted protamine‑like protein |
? | AP012306 | 1092985 = | 291 (1.050) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 997497 | 288 (0.900) | 12 (0.040) | 12/268 | 10.3 | 4.3% | coding (41/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
? | AP012306 | 997993 = | 275 (0.950) | coding (537/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase | |||||
* | ? | AP012306 | = 1092334 | 324 (1.010) | 12 (0.040) | 11/258 | 10.4 | 4.0% | coding (198/276 nt) | ychS | predicted protein |
? | AP012306 | 1092985 = | 291 (1.050) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 215364 | NA (NA) | 9 (0.030) | 9/288 | 11.5 | NA | noncoding (745/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) |
? | AP012306 | = 215372 | NA (NA) | noncoding (753/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) | |||||
* | ? | AP012306 | = 1616258 | 343 (1.070) | 7 (0.020) | 7/274 | 12.0 | 2.2% | coding (203/1476 nt) | zwf | glucose‑6‑phosphate dehydrogenase |
? | AP012306 | = 1616246 | 310 (1.050) | coding (215/1476 nt) | zwf | glucose‑6‑phosphate dehydrogenase | |||||
* | ? | AP012306 | = 5455 | 298 (0.930) | 8 (0.030) | 7/282 | 12.1 | 2.9% | coding (222/297 nt) | yaaX | predicted protein |
? | AP012306 | = 5496 | 256 (0.840) | coding (263/297 nt) | yaaX | predicted protein | |||||
* | ? | AP012306 | 2424031 = | 311 (0.970) | 6 (0.020) | 6/270 | 12.3 | 2.1% | coding (156/2235 nt) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
? | AP012306 | 2424045 = | 287 (0.990) | coding (142/2235 nt) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase | |||||
* | ? | AP012306 | 1824798 = | 281 (0.870) | 6 (0.020) | 6/274 | 12.4 | 2.3% | coding (935/1050 nt) | yeiH | conserved inner membrane protein |
? | AP012306 | 1824810 = | 261 (0.880) | coding (947/1050 nt) | yeiH | conserved inner membrane protein | |||||
* | ? | AP012306 | = 2245969 | 466 (1.450) | 6 (0.020) | 6/288 | 12.6 | 1.3% | intergenic (+419/+276) | yfhL/acpS | predicted 4Fe‑4S cluster‑containing protein/holo‑[acyl‑carrier‑protein] synthase 1 |
? | AP012306 | = 2245982 | 444 (1.430) | intergenic (+432/+263) | yfhL/acpS | predicted 4Fe‑4S cluster‑containing protein/holo‑[acyl‑carrier‑protein] synthase 1 | |||||
* | ? | AP012306 | = 1458396 | 378 (1.180) | 6 (0.020) | 6/294 | 12.7 | 1.6% | coding (1227/1596 nt) | ydiF | fused predicted acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit |
? | AP012306 | = 1458413 | 360 (1.140) | coding (1244/1596 nt) | ydiF | fused predicted acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit |