breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 30 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG45.1% 399.0 / 81.1 273intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC38.3% 343.3 / 45.6 188G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,527,3680AC32.0% 588.5 / 73.7 275L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306644,4170GA31.6% 853.5 / inf 593intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP012306341,3770TG31.1% 203.9 / 24.3 124G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123062,758,9090TG31.1% 539.4 / 33.5 235G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP012306800,6750AC30.3% 565.3 / 44.6 254T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123063,590,3670AC28.2% 663.8 / 40.4 267T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP0123062,714,5550TG27.2% 656.1 / 24.4 285T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP012306257,1370TG26.3% 379.4 / 10.1 179L496V (TTG→GTG) betTcholine transporter of high affinity
*AP0123063,903,7910AG26.0% 616.9 / 72.5 266E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123061,066,3230AC25.8% 715.6 / 23.2 271intergenic (‑92/+33)ychM/prsApredicted transporter/phosphoribosylpyrophosphate synthase
*AP0123061,854,8510AC25.5% 765.3 / 59.5 291T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123063,938,8870CT25.1% 580.9 / 138.2 267S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123063,639,0400TG24.7% 166.4 / 15.4 77noncoding (54/76 nt)gltVtRNA‑Glu
*AP0123062,212,7530AC24.1% 343.2 / 41.8 133Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123063,282,0760TG23.8% 564.3 / 15.2 244intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide
*AP012306202,4170TG23.5% 676.4 / 27.7 278V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123061,074,6190TC23.2% 364.2 / 99.3 194S29G (AGC→GGC) ldrAtoxic polypeptide, small
*AP0123063,645,6420AC23.0% 832.0 / 30.0 313T318P (ACC→CCC) aceBmalate synthase A

Marginal new junction evidence (lowest skew 10 of 143 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 1092512382 (1.190)25 (0.090) 15/258 9.4 7.4% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 291 (1.050)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 997497288 (0.900)12 (0.040) 12/268 10.3 4.3% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 275 (0.950)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 1092334324 (1.010)12 (0.040) 11/258 10.4 4.0% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 291 (1.050)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 215364NA (NA)9 (0.030) 9/288 11.5 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 1616258343 (1.070)7 (0.020) 7/274 12.0 2.2% coding (203/1476 nt) zwf glucose‑6‑phosphate dehydrogenase
?AP012306 = 1616246 310 (1.050)coding (215/1476 nt) zwf glucose‑6‑phosphate dehydrogenase
* ? AP012306 = 5455298 (0.930)8 (0.030) 7/282 12.1 2.9% coding (222/297 nt) yaaX predicted protein
?AP012306 = 5496 256 (0.840)coding (263/297 nt) yaaX predicted protein
* ? AP012306 2424031 =311 (0.970)6 (0.020) 6/270 12.3 2.1% coding (156/2235 nt) relA (p)ppGpp synthetase I/GTP pyrophosphokinase
?AP012306 2424045 = 287 (0.990)coding (142/2235 nt) relA (p)ppGpp synthetase I/GTP pyrophosphokinase
* ? AP012306 1824798 =281 (0.870)6 (0.020) 6/274 12.4 2.3% coding (935/1050 nt) yeiH conserved inner membrane protein
?AP012306 1824810 = 261 (0.880)coding (947/1050 nt) yeiH conserved inner membrane protein
* ? AP012306 = 2245969466 (1.450)6 (0.020) 6/288 12.6 1.3% intergenic (+419/+276) yfhL/acpS predicted 4Fe‑4S cluster‑containing protein/holo‑[acyl‑carrier‑protein] synthase 1
?AP012306 = 2245982 444 (1.430)intergenic (+432/+263) yfhL/acpS predicted 4Fe‑4S cluster‑containing protein/holo‑[acyl‑carrier‑protein] synthase 1
* ? AP012306 = 1458396378 (1.180)6 (0.020) 6/294 12.7 1.6% coding (1227/1596 nt) ydiF fused predicted acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
?AP012306 = 1458413 360 (1.140)coding (1244/1596 nt) ydiF fused predicted acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit