breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG41.1% 411.6 / 79.9 255intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC36.4% 387.2 / 64.9 215G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306341,3770TG33.1% 216.3 / 25.9 127G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP012306644,4170GA31.7% 738.7 / inf 511intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,527,3680AC30.5% 538.3 / 55.3 239L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123063,938,8870CT30.0% 377.1 / 138.8 213S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123061,421,1470TG29.1% 453.6 / 15.0 235T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
*AP012306800,6750AC28.8% 540.0 / 35.6 243T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123062,714,5550TG27.9% 610.9 / 13.6 271T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP0123062,758,9090TG27.5% 580.7 / 17.6 244G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123061,226,5570AG24.5% 462.7 / 87.2 204G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123062,212,7530AC23.9% 428.1 / 63.7 176Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP012306406,6770AG23.8% 602.3 / 24.4 239K294E (AAA→GAA) hemHferrochelatase
*AP0123061,854,8510AC23.8% 717.7 / 64.0 286T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123063,182,8610AC23.8% 307.7 / 14.6 122L198F (TTA→TTCyiaNpredicted transporter
*AP0123063,020,8840TG23.4% 635.1 / 11.7 244N124T (AAC→ACC) glgCglucose‑1‑phosphate adenylyltransferase
*AP0123063,324,4330TA23.2% 632.8 / 103.0 259intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
*AP0123061,128,4120TG22.7% 670.0 / 35.9 264V29G (GTG→GGG) yciOconserved protein
*AP0123063,412,0490TG22.4% 831.0 / 38.4 335intergenic (+25/‑122)proM/aslBtRNA‑Pro/predicted regulator of arylsulfatase activity
*AP0123062,680,3450AC22.1% 467.8 / 22.0 190T184P (ACC→CCC) ttdAL‑tartrate dehydratase, alpha subunit

Marginal new junction evidence (lowest skew 10 of 69 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 644310 =347 (1.090)44 (0.140)
+GG
21/294 12.5 11.5% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073420 340 (1.070)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 644310 =347 (1.090)41 (0.130)
+G
17/296 13.6 10.9% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073541 329 (1.040)intergenic (+1/‑46) valX/valY tRNA‑Val/tRNA‑Val
* ? AP012306 = 997497307 (0.970)14 (0.050) 13/268 14.2 4.6% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 303 (1.060)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 1092512376 (1.190)18 (0.070) 10/258 15.0 5.6% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 282 (1.030)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1092334331 (1.040)10 (0.040) 9/258 15.4 3.4% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 282 (1.030)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 644310 =347 (1.090)25 (0.080)
+G
9/296 16.4 6.9% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073542 331 (1.040)intergenic (+2/‑45) valX/valY tRNA‑Val/tRNA‑Val
* ? AP012306 1054958 =255 (0.800)6 (0.020) 6/284 17.5 2.3% coding (228/633 nt) dhaL dihydroxyacetone kinase, C‑terminal domain
?AP012306 1054977 = 257 (0.850)coding (209/633 nt) dhaL dihydroxyacetone kinase, C‑terminal domain
* ? AP012306 2184861 =332 (1.050)6 (0.020) 6/288 17.6 1.8% coding (50/621 nt) yfgM conserved protein
?AP012306 2184877 = 322 (1.050)coding (34/621 nt) yfgM conserved protein
* ? AP012306 759114 =266 (0.840)6 (0.020) 6/288 17.6 2.3% coding (248/954 nt) potH putrescine transporter subunit
?AP012306 759139 = 257 (0.840)coding (273/954 nt) potH putrescine transporter subunit
* ? AP012306 = 213957NA (NA)6 (0.020) 6/288 17.6 NA noncoding (1326/1542 nt) rrsH 16S ribosomal RNA (rrsH)
?AP012306 = 213963 NA (NA)noncoding (1332/1542 nt) rrsH 16S ribosomal RNA (rrsH)