breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 41.1% | 411.6 / 79.9 | 255 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 1,944,288 | 0 | A | C | 36.4% | 387.2 / 64.9 | 215 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 341,377 | 0 | T | G | 33.1% | 216.3 / 25.9 | 127 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 644,417 | 0 | G | A | 31.7% | 738.7 / inf | 511 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 3,527,368 | 0 | A | C | 30.5% | 538.3 / 55.3 | 239 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 3,938,887 | 0 | C | T | 30.0% | 377.1 / 138.8 | 213 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 1,421,147 | 0 | T | G | 29.1% | 453.6 / 15.0 | 235 | T79P (ACC→CCC) | ydhB | predicted DNA‑binding transcriptional regulator |
* | AP012306 | 800,675 | 0 | A | C | 28.8% | 540.0 / 35.6 | 243 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 2,714,555 | 0 | T | G | 27.9% | 610.9 / 13.6 | 271 | T175P (ACC→CCC) | ygjV | conserved inner membrane protein |
* | AP012306 | 2,758,909 | 0 | T | G | 27.5% | 580.7 / 17.6 | 244 | G33G (GGT→GGG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
* | AP012306 | 1,226,557 | 0 | A | G | 24.5% | 462.7 / 87.2 | 204 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 2,212,753 | 0 | A | C | 23.9% | 428.1 / 63.7 | 176 | Y241D (TAT→GAT) | hcaT | predicted 3‑phenylpropionic transporter |
* | AP012306 | 406,677 | 0 | A | G | 23.8% | 602.3 / 24.4 | 239 | K294E (AAA→GAA) | hemH | ferrochelatase |
* | AP012306 | 1,854,851 | 0 | A | C | 23.8% | 717.7 / 64.0 | 286 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 3,182,861 | 0 | A | C | 23.8% | 307.7 / 14.6 | 122 | L198F (TTA→TTC) | yiaN | predicted transporter |
* | AP012306 | 3,020,884 | 0 | T | G | 23.4% | 635.1 / 11.7 | 244 | N124T (AAC→ACC) | glgC | glucose‑1‑phosphate adenylyltransferase |
* | AP012306 | 3,324,433 | 0 | T | A | 23.2% | 632.8 / 103.0 | 259 | intergenic (+2/+52) | yieF/yieG | chromate reductase, Class I, flavoprotein/predicted inner membrane protein |
* | AP012306 | 1,128,412 | 0 | T | G | 22.7% | 670.0 / 35.9 | 264 | V29G (GTG→GGG) | yciO | conserved protein |
* | AP012306 | 3,412,049 | 0 | T | G | 22.4% | 831.0 / 38.4 | 335 | intergenic (+25/‑122) | proM/aslB | tRNA‑Pro/predicted regulator of arylsulfatase activity |
* | AP012306 | 2,680,345 | 0 | A | C | 22.1% | 467.8 / 22.0 | 190 | T184P (ACC→CCC) | ttdA | L‑tartrate dehydratase, alpha subunit |
Marginal new junction evidence (lowest skew 10 of 69 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | 644310 = | 347 (1.090) | 44 (0.140) +GG |
21/294 | 12.5 | 11.5% | noncoding (1/76 nt) | lysQ | tRNA‑Lys |
? | AP012306 | = 2073420 | 340 (1.070) | noncoding (76/76 nt) | valU | tRNA‑Val | |||||
* | ? | AP012306 | 644310 = | 347 (1.090) | 41 (0.130) +G |
17/296 | 13.6 | 10.9% | noncoding (1/76 nt) | lysQ | tRNA‑Lys |
? | AP012306 | = 2073541 | 329 (1.040) | intergenic (+1/‑46) | valX/valY | tRNA‑Val/tRNA‑Val | |||||
* | ? | AP012306 | = 997497 | 307 (0.970) | 14 (0.050) | 13/268 | 14.2 | 4.6% | coding (41/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
? | AP012306 | 997993 = | 303 (1.060) | coding (537/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase | |||||
* | ? | AP012306 | = 1092512 | 376 (1.190) | 18 (0.070) | 10/258 | 15.0 | 5.6% | intergenic (+100/+3) | ychS/tpr | predicted protein/predicted protamine‑like protein |
? | AP012306 | 1092985 = | 282 (1.030) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 1092334 | 331 (1.040) | 10 (0.040) | 9/258 | 15.4 | 3.4% | coding (198/276 nt) | ychS | predicted protein |
? | AP012306 | 1092985 = | 282 (1.030) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | 644310 = | 347 (1.090) | 25 (0.080) +G |
9/296 | 16.4 | 6.9% | noncoding (1/76 nt) | lysQ | tRNA‑Lys |
? | AP012306 | = 2073542 | 331 (1.040) | intergenic (+2/‑45) | valX/valY | tRNA‑Val/tRNA‑Val | |||||
* | ? | AP012306 | 1054958 = | 255 (0.800) | 6 (0.020) | 6/284 | 17.5 | 2.3% | coding (228/633 nt) | dhaL | dihydroxyacetone kinase, C‑terminal domain |
? | AP012306 | 1054977 = | 257 (0.850) | coding (209/633 nt) | dhaL | dihydroxyacetone kinase, C‑terminal domain | |||||
* | ? | AP012306 | 2184861 = | 332 (1.050) | 6 (0.020) | 6/288 | 17.6 | 1.8% | coding (50/621 nt) | yfgM | conserved protein |
? | AP012306 | 2184877 = | 322 (1.050) | coding (34/621 nt) | yfgM | conserved protein | |||||
* | ? | AP012306 | 759114 = | 266 (0.840) | 6 (0.020) | 6/288 | 17.6 | 2.3% | coding (248/954 nt) | potH | putrescine transporter subunit |
? | AP012306 | 759139 = | 257 (0.840) | coding (273/954 nt) | potH | putrescine transporter subunit | |||||
* | ? | AP012306 | = 213957 | NA (NA) | 6 (0.020) | 6/288 | 17.6 | NA | noncoding (1326/1542 nt) | rrsH | 16S ribosomal RNA (rrsH) |
? | AP012306 | = 213963 | NA (NA) | noncoding (1332/1542 nt) | rrsH | 16S ribosomal RNA (rrsH) |