breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 42.9% | 439.3 / 85.8 | 282 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 341,377 | 0 | T | G | 33.6% | 222.5 / 20.3 | 129 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 644,417 | 0 | G | A | 31.6% | 754.5 / inf | 507 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 3,527,368 | 0 | A | C | 30.5% | 517.0 / 40.7 | 226 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 935,054 | 0 | G | T | 30.4% | 217.8 / 52.0 | 92 | intergenic (+24/‑1406) | putP/ycdO | proline:sodium symporter/conserved protein |
* | AP012306 | 1,944,288 | 0 | A | C | 30.0% | 471.3 / 45.5 | 221 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 3,938,887 | 0 | C | T | 28.3% | 421.8 / 121.0 | 212 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 3,182,861 | 0 | A | C | 27.4% | 300.8 / 14.9 | 124 | L198F (TTA→TTC) | yiaN | predicted transporter |
* | AP012306 | 1,854,851 | 0 | A | C | 27.4% | 602.2 / 67.7 | 259 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 1,226,557 | 0 | A | G | 27.3% | 414.0 / 82.6 | 195 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 800,675 | 0 | A | C | 26.9% | 532.5 / 31.7 | 239 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 2,212,753 | 0 | A | C | 26.3% | 376.0 / 35.4 | 152 | Y241D (TAT→GAT) | hcaT | predicted 3‑phenylpropionic transporter |
* | AP012306 | 3,639,040 | 0 | T | G | 26.3% | 158.6 / 17.5 | 76 | noncoding (54/76 nt) | gltV | tRNA‑Glu |
* | AP012306 | 1,309,914 | 0 | A | C | 25.0% | 298.4 / 12.0 | 145 | L23F (TTA→TTC) | lsrC | AI2 transporter |
* | AP012306 | 3,590,361 | 0 | G | A | 23.1% | 644.5 / 108.5 | 260 | E28K (GAA→AAA) | fabR | DNA‑binding transcriptional repressor |
* | AP012306 | 3,324,433 | 0 | T | A | 22.7% | 635.4 / 90.5 | 251 | intergenic (+2/+52) | yieF/yieG | chromate reductase, Class I, flavoprotein/predicted inner membrane protein |
* | AP012306 | 3,590,367 | 0 | A | C | 22.0% | 714.5 / 25.8 | 260 | T30P (ACC→CCC) | fabR | DNA‑binding transcriptional repressor |
* | AP012306 | 202,417 | 0 | T | G | 22.0% | 711.5 / 16.1 | 266 | V409G (GTA→GGA) | tilS | tRNA(Ile)‑lysidine synthetase |
* | AP012306 | 1,128,412 | 0 | T | G | 21.7% | 669.9 / 31.6 | 254 | V29G (GTG→GGG) | yciO | conserved protein |
* | AP012306 | 3,903,371 | 0 | A | C | 21.5% | 778.2 / 34.0 | 307 | N49T (AAC→ACC) | yjgJ | predicted transcriptional regulator |
Marginal new junction evidence (lowest skew 10 of 65 shown) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | = 997497 | 299 (1.010) | 13 (0.050) | 13/268 | 18.3 | 5.0% | coding (41/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
? | AP012306 | 997993 = | 228 (0.860) | coding (537/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase | |||||
* | ? | AP012306 | = 1092512 | 372 (1.260) | 11 (0.040) | 7/258 | 21.0 | 3.7% | intergenic (+100/+3) | ychS/tpr | predicted protein/predicted protamine‑like protein |
? | AP012306 | 1092985 = | 258 (1.010) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 1210600 | 292 (0.990) | 8 (0.030) | 7/284 | 21.9 | 2.7% | coding (1153/1626 nt) | mdoD | glucan biosynthesis protein, periplasmic |
? | AP012306 | = 1210601 | 292 (1.040) | coding (1154/1626 nt) | mdoD | glucan biosynthesis protein, periplasmic | |||||
* | ? | AP012306 | = 3503491 | 311 (1.050) | 7 (0.020) | 7/298 | 22.3 | 2.2% | coding (540/897 nt) | yihV | predicted sugar kinase |
? | AP012306 | = 3503507 | 326 (1.100) | coding (556/897 nt) | yihV | predicted sugar kinase | |||||
* | ? | AP012306 | = 3868258 | 311 (1.050) | 6 (0.020) | 6/288 | 22.6 | 1.9% | coding (107/555 nt) | ytfJ | predicted transcriptional regulator |
? | AP012306 | = 3868269 | 314 (1.100) | coding (96/555 nt) | ytfJ | predicted transcriptional regulator | |||||
* | ? | AP012306 | 1856463 = | 213 (0.720) | 5 (0.020) | 5/274 | 22.8 | 2.5% | coding (1685/1761 nt) | yejH | predicted ATP‑dependet helicase |
? | AP012306 | 1856475 = | 200 (0.740) | coding (1697/1761 nt) | yejH | predicted ATP‑dependet helicase | |||||
* | ? | AP012306 | 2898619 = | 319 (1.080) | 5 (0.020) | 5/284 | 23.2 | 1.6% | coding (72/819 nt) | aroE | dehydroshikimate reductase, NAD(P)‑binding |
? | AP012306 | 2898652 = | 308 (1.090) | coding (39/819 nt) | aroE | dehydroshikimate reductase, NAD(P)‑binding | |||||
* | ? | AP012306 | = 3377548 | 296 (1.000) | 5 (0.020) | 5/288 | 23.3 | 1.8% | coding (250/339 nt) | yifE | conserved protein |
? | AP012306 | = 3377555 | 274 (0.960) | coding (257/339 nt) | yifE | conserved protein | |||||
* | ? | AP012306 | = 3143742 | 208 (0.700) | 5 (0.020) | 5/288 | 23.3 | 2.4% | coding (956/1020 nt) | dppB | dipeptide transporter |
? | AP012306 | = 3143751 | 206 (0.720) | coding (947/1020 nt) | dppB | dipeptide transporter | |||||
* | ? | AP012306 | 785238 = | 312 (1.050) | 6 (0.020) | 5/288 | 23.3 | 2.0% | coding (86/225 nt) | cspD | cold shock protein homolog |
? | AP012306 | 785254 = | 289 (1.010) | coding (70/225 nt) | cspD | cold shock protein homolog |