breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG42.9% 439.3 / 85.8 282intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP012306341,3770TG33.6% 222.5 / 20.3 129G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP012306644,4170GA31.6% 754.5 / inf 507intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,527,3680AC30.5% 517.0 / 40.7 226L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306935,0540GT30.4% 217.8 / 52.0 92intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123061,944,2880AC30.0% 471.3 / 45.5 221G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,938,8870CT28.3% 421.8 / 121.0 212S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123063,182,8610AC27.4% 300.8 / 14.9 124L198F (TTA→TTCyiaNpredicted transporter
*AP0123061,854,8510AC27.4% 602.2 / 67.7 259T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123061,226,5570AG27.3% 414.0 / 82.6 195G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP012306800,6750AC26.9% 532.5 / 31.7 239T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123062,212,7530AC26.3% 376.0 / 35.4 152Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123063,639,0400TG26.3% 158.6 / 17.5 76noncoding (54/76 nt)gltVtRNA‑Glu
*AP0123061,309,9140AC25.0% 298.4 / 12.0 145L23F (TTA→TTClsrCAI2 transporter
*AP0123063,590,3610GA23.1% 644.5 / 108.5 260E28K (GAA→AAA) fabRDNA‑binding transcriptional repressor
*AP0123063,324,4330TA22.7% 635.4 / 90.5 251intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
*AP0123063,590,3670AC22.0% 714.5 / 25.8 260T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP012306202,4170TG22.0% 711.5 / 16.1 266V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123061,128,4120TG21.7% 669.9 / 31.6 254V29G (GTG→GGG) yciOconserved protein
*AP0123063,903,3710AC21.5% 778.2 / 34.0 307N49T (AAC→ACC) yjgJpredicted transcriptional regulator

Marginal new junction evidence (lowest skew 10 of 65 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 997497299 (1.010)13 (0.050) 13/268 18.3 5.0% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 228 (0.860)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 1092512372 (1.260)11 (0.040) 7/258 21.0 3.7% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 258 (1.010)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1210600292 (0.990)8 (0.030) 7/284 21.9 2.7% coding (1153/1626 nt) mdoD glucan biosynthesis protein, periplasmic
?AP012306 = 1210601 292 (1.040)coding (1154/1626 nt) mdoD glucan biosynthesis protein, periplasmic
* ? AP012306 = 3503491311 (1.050)7 (0.020) 7/298 22.3 2.2% coding (540/897 nt) yihV predicted sugar kinase
?AP012306 = 3503507 326 (1.100)coding (556/897 nt) yihV predicted sugar kinase
* ? AP012306 = 3868258311 (1.050)6 (0.020) 6/288 22.6 1.9% coding (107/555 nt) ytfJ predicted transcriptional regulator
?AP012306 = 3868269 314 (1.100)coding (96/555 nt) ytfJ predicted transcriptional regulator
* ? AP012306 1856463 =213 (0.720)5 (0.020) 5/274 22.8 2.5% coding (1685/1761 nt) yejH predicted ATP‑dependet helicase
?AP012306 1856475 = 200 (0.740)coding (1697/1761 nt) yejH predicted ATP‑dependet helicase
* ? AP012306 2898619 =319 (1.080)5 (0.020) 5/284 23.2 1.6% coding (72/819 nt) aroE dehydroshikimate reductase, NAD(P)‑binding
?AP012306 2898652 = 308 (1.090)coding (39/819 nt) aroE dehydroshikimate reductase, NAD(P)‑binding
* ? AP012306 = 3377548296 (1.000)5 (0.020) 5/288 23.3 1.8% coding (250/339 nt) yifE conserved protein
?AP012306 = 3377555 274 (0.960)coding (257/339 nt) yifE conserved protein
* ? AP012306 = 3143742208 (0.700)5 (0.020) 5/288 23.3 2.4% coding (956/1020 nt) dppB dipeptide transporter
?AP012306 = 3143751 206 (0.720)coding (947/1020 nt) dppB dipeptide transporter
* ? AP012306 785238 =312 (1.050)6 (0.020) 5/288 23.3 2.0% coding (86/225 nt) cspD cold shock protein homolog
?AP012306 785254 = 289 (1.010)coding (70/225 nt) cspD cold shock protein homolog